Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury

Autores
Meffre, D.; Labombarda, Maria Florencia; Delespierre, B.; Chastre, A.; de Nicola, Alejandro Federico; Stein, D. G.; Schumacher, Michael; Guennoun, Rachida
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Progesterone has been shown to exert pleiotropic actions in the brain of both male and females. In particular, after traumatic brain injury (TBI), progesterone has important neuroprotective effects. In addition to intracellular progesterone receptors, membrane receptors of the hormone such as membrane progesterone receptor (mPR) may also be involved in neuroprotection. Three mPR subtypes (mPRα, mPRβ, and mPRγ) have been described and mPRα is best characterized pharmacologically. In the present study we investigated the distribution, cellular localization and the regulation of mPRα in male mouse and rat brain. We showed by reverse transcription-PCR that mPRα is expressed at similar levels in the male and female mouse brain suggesting that its expression may not be influenced by steroid levels. Treatment of males by estradiol or progesterone did not modify the level of expression of mPRα as shown by Western blot analysis. In situhybridization and immunohistochemistry analysis showed a wide expression of mPRα in particular in the olfactory bulb, striatum, cortex, thalamus, hypothalamus, septum, hippocampus and cerebellum. Double immunofluorescence and confocal microscopy analysis showed that mPRα is expressed by neurons but not by oligodendrocytes and astrocytes. In the rat brain, the distribution of mPRα was similar to that observed in mouse brain; and after TBI, mPRα expression was induced in oligodendrocytes, astrocytes and reactive microglia. The wide neuroanatomical distribution of mPRα suggests that this receptor may play a role beyond neuroendocrine and reproductive functions. However, in the absence of injury its role might be restricted to neurons. The induction of mPRα after TBI in microglia, astrocytes and oligodendrocytes, points to a potential role in mediating the modulatory effects of progesterone in inflammation, ion and water homeostasis and myelin repair in the injured brain.
Fil: Meffre, D.. Inserm; Francia
Fil: Labombarda, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Delespierre, B.. Inserm; Francia
Fil: Chastre, A.. Inserm; Francia
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Stein, D. G.. University of Emory; Estados Unidos
Fil: Schumacher, Michael. Inserm; Francia
Fil: Guennoun, Rachida. Inserm; Francia
Materia
ASTROCYTES
MEMBRANE PROGESTERONE RECEPTOR (MPR)
MICROGLIA
NEURONS
OLIGODENDROCYTES
TRAUMATIC BRAIN INJURY (TBI)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2180
Acceder
id CONICETDig_238493b9fa9d6c322640b3a25e4bb8e6
oai_identifier_str oai:ri.conicet.gov.ar:11336/2180
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injuryMeffre, D.Labombarda, Maria FlorenciaDelespierre, B.Chastre, A.de Nicola, Alejandro FedericoStein, D. G.Schumacher, MichaelGuennoun, RachidaASTROCYTESMEMBRANE PROGESTERONE RECEPTOR (MPR)MICROGLIANEURONSOLIGODENDROCYTESTRAUMATIC BRAIN INJURY (TBI)https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Progesterone has been shown to exert pleiotropic actions in the brain of both male and females. In particular, after traumatic brain injury (TBI), progesterone has important neuroprotective effects. In addition to intracellular progesterone receptors, membrane receptors of the hormone such as membrane progesterone receptor (mPR) may also be involved in neuroprotection. Three mPR subtypes (mPRα, mPRβ, and mPRγ) have been described and mPRα is best characterized pharmacologically. In the present study we investigated the distribution, cellular localization and the regulation of mPRα in male mouse and rat brain. We showed by reverse transcription-PCR that mPRα is expressed at similar levels in the male and female mouse brain suggesting that its expression may not be influenced by steroid levels. Treatment of males by estradiol or progesterone did not modify the level of expression of mPRα as shown by Western blot analysis. In situhybridization and immunohistochemistry analysis showed a wide expression of mPRα in particular in the olfactory bulb, striatum, cortex, thalamus, hypothalamus, septum, hippocampus and cerebellum. Double immunofluorescence and confocal microscopy analysis showed that mPRα is expressed by neurons but not by oligodendrocytes and astrocytes. In the rat brain, the distribution of mPRα was similar to that observed in mouse brain; and after TBI, mPRα expression was induced in oligodendrocytes, astrocytes and reactive microglia. The wide neuroanatomical distribution of mPRα suggests that this receptor may play a role beyond neuroendocrine and reproductive functions. However, in the absence of injury its role might be restricted to neurons. The induction of mPRα after TBI in microglia, astrocytes and oligodendrocytes, points to a potential role in mediating the modulatory effects of progesterone in inflammation, ion and water homeostasis and myelin repair in the injured brain.Fil: Meffre, D.. Inserm; FranciaFil: Labombarda, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Delespierre, B.. Inserm; FranciaFil: Chastre, A.. Inserm; FranciaFil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Stein, D. G.. University of Emory; Estados UnidosFil: Schumacher, Michael. Inserm; FranciaFil: Guennoun, Rachida. Inserm; FranciaPergamon-Elsevier Science Ltd2013-02-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2180Meffre, D.; Labombarda, Maria Florencia; Delespierre, B.; Chastre, A.; de Nicola, Alejandro Federico; et al.; Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury; Pergamon-Elsevier Science Ltd; Neuroscience; 231; 12-2-2013; 111-1240306-45221873-7544enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0306452212011505info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2012.11.039info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:56Zoai:ri.conicet.gov.ar:11336/2180instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:57.003CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury
title Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury
spellingShingle Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury
Meffre, D.
ASTROCYTES
MEMBRANE PROGESTERONE RECEPTOR (MPR)
MICROGLIA
NEURONS
OLIGODENDROCYTES
TRAUMATIC BRAIN INJURY (TBI)
title_short Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury
title_full Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury
title_fullStr Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury
title_full_unstemmed Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury
title_sort Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury
dc.creator.none.fl_str_mv Meffre, D.
Labombarda, Maria Florencia
Delespierre, B.
Chastre, A.
de Nicola, Alejandro Federico
Stein, D. G.
Schumacher, Michael
Guennoun, Rachida
author Meffre, D.
author_facet Meffre, D.
Labombarda, Maria Florencia
Delespierre, B.
Chastre, A.
de Nicola, Alejandro Federico
Stein, D. G.
Schumacher, Michael
Guennoun, Rachida
author_role author
author2 Labombarda, Maria Florencia
Delespierre, B.
Chastre, A.
de Nicola, Alejandro Federico
Stein, D. G.
Schumacher, Michael
Guennoun, Rachida
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ASTROCYTES
MEMBRANE PROGESTERONE RECEPTOR (MPR)
MICROGLIA
NEURONS
OLIGODENDROCYTES
TRAUMATIC BRAIN INJURY (TBI)
topic ASTROCYTES
MEMBRANE PROGESTERONE RECEPTOR (MPR)
MICROGLIA
NEURONS
OLIGODENDROCYTES
TRAUMATIC BRAIN INJURY (TBI)
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Progesterone has been shown to exert pleiotropic actions in the brain of both male and females. In particular, after traumatic brain injury (TBI), progesterone has important neuroprotective effects. In addition to intracellular progesterone receptors, membrane receptors of the hormone such as membrane progesterone receptor (mPR) may also be involved in neuroprotection. Three mPR subtypes (mPRα, mPRβ, and mPRγ) have been described and mPRα is best characterized pharmacologically. In the present study we investigated the distribution, cellular localization and the regulation of mPRα in male mouse and rat brain. We showed by reverse transcription-PCR that mPRα is expressed at similar levels in the male and female mouse brain suggesting that its expression may not be influenced by steroid levels. Treatment of males by estradiol or progesterone did not modify the level of expression of mPRα as shown by Western blot analysis. In situhybridization and immunohistochemistry analysis showed a wide expression of mPRα in particular in the olfactory bulb, striatum, cortex, thalamus, hypothalamus, septum, hippocampus and cerebellum. Double immunofluorescence and confocal microscopy analysis showed that mPRα is expressed by neurons but not by oligodendrocytes and astrocytes. In the rat brain, the distribution of mPRα was similar to that observed in mouse brain; and after TBI, mPRα expression was induced in oligodendrocytes, astrocytes and reactive microglia. The wide neuroanatomical distribution of mPRα suggests that this receptor may play a role beyond neuroendocrine and reproductive functions. However, in the absence of injury its role might be restricted to neurons. The induction of mPRα after TBI in microglia, astrocytes and oligodendrocytes, points to a potential role in mediating the modulatory effects of progesterone in inflammation, ion and water homeostasis and myelin repair in the injured brain.
Fil: Meffre, D.. Inserm; Francia
Fil: Labombarda, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Delespierre, B.. Inserm; Francia
Fil: Chastre, A.. Inserm; Francia
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Stein, D. G.. University of Emory; Estados Unidos
Fil: Schumacher, Michael. Inserm; Francia
Fil: Guennoun, Rachida. Inserm; Francia
description Progesterone has been shown to exert pleiotropic actions in the brain of both male and females. In particular, after traumatic brain injury (TBI), progesterone has important neuroprotective effects. In addition to intracellular progesterone receptors, membrane receptors of the hormone such as membrane progesterone receptor (mPR) may also be involved in neuroprotection. Three mPR subtypes (mPRα, mPRβ, and mPRγ) have been described and mPRα is best characterized pharmacologically. In the present study we investigated the distribution, cellular localization and the regulation of mPRα in male mouse and rat brain. We showed by reverse transcription-PCR that mPRα is expressed at similar levels in the male and female mouse brain suggesting that its expression may not be influenced by steroid levels. Treatment of males by estradiol or progesterone did not modify the level of expression of mPRα as shown by Western blot analysis. In situhybridization and immunohistochemistry analysis showed a wide expression of mPRα in particular in the olfactory bulb, striatum, cortex, thalamus, hypothalamus, septum, hippocampus and cerebellum. Double immunofluorescence and confocal microscopy analysis showed that mPRα is expressed by neurons but not by oligodendrocytes and astrocytes. In the rat brain, the distribution of mPRα was similar to that observed in mouse brain; and after TBI, mPRα expression was induced in oligodendrocytes, astrocytes and reactive microglia. The wide neuroanatomical distribution of mPRα suggests that this receptor may play a role beyond neuroendocrine and reproductive functions. However, in the absence of injury its role might be restricted to neurons. The induction of mPRα after TBI in microglia, astrocytes and oligodendrocytes, points to a potential role in mediating the modulatory effects of progesterone in inflammation, ion and water homeostasis and myelin repair in the injured brain.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2180
Meffre, D.; Labombarda, Maria Florencia; Delespierre, B.; Chastre, A.; de Nicola, Alejandro Federico; et al.; Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury; Pergamon-Elsevier Science Ltd; Neuroscience; 231; 12-2-2013; 111-124
0306-4522
1873-7544
url http://hdl.handle.net/11336/2180
identifier_str_mv Meffre, D.; Labombarda, Maria Florencia; Delespierre, B.; Chastre, A.; de Nicola, Alejandro Federico; et al.; Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury; Pergamon-Elsevier Science Ltd; Neuroscience; 231; 12-2-2013; 111-124
0306-4522
1873-7544
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0306452212011505
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2012.11.039
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268634718666752
score 13.13397

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