Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei

Autores
Mandal, Pinaki; Pujol, Carlos Alberto; Carlucci, Maria Josefina; Chattopadhyay, Kausik; Damonte, Elsa Beatriz; Ray, Bimalendu
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study we have analyzed polysaccharide fractions isolated from Scinaia hatei. The crude water extract (ShWE) as well as one fraction (F1) obtained by size exclusion chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values ranging from 0.5 to 4.6 μg/ml were much lower than the cytotoxic concentration 50% (CC50) values (≥1000 μg/ml). These fractions had very low anticoagulant activity. Furthermore, they had a weak inactivating effect on virions in a virucidal assay at concentrations in the range of 60-100 μg/ml. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide, which had 0.4 sulfate group per monomer unit and an apparent molecular mass of 160 kDa, contained a backbone of α-(1 → 3)-linked d-mannopyranosyl residues substituted at C-6, C-4 and C-2 with single stub of β-d-xylopyranosyl residues. Sulfate groups, when present, are located at C-4 of α-(1 → 3)-linked d-mannopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer.
Fil: Mandal, Pinaki. University of Burdwan; India
Fil: Pujol, Carlos Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carlucci, Maria Josefina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chattopadhyay, Kausik. University of Burdwan; India
Fil: Damonte, Elsa Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ray, Bimalendu. University of Burdwan; India
Materia
Antiviral Activity
Herpes Simplex Virus
Liagoraceae
Scinaia Hatei
Xylomannan Sulfate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/74923
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anti-herpetic activity of a sulfated xylomannan from Scinaia hateiMandal, PinakiPujol, Carlos AlbertoCarlucci, Maria JosefinaChattopadhyay, KausikDamonte, Elsa BeatrizRay, BimalenduAntiviral ActivityHerpes Simplex VirusLiagoraceaeScinaia HateiXylomannan Sulfatehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study we have analyzed polysaccharide fractions isolated from Scinaia hatei. The crude water extract (ShWE) as well as one fraction (F1) obtained by size exclusion chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values ranging from 0.5 to 4.6 μg/ml were much lower than the cytotoxic concentration 50% (CC50) values (≥1000 μg/ml). These fractions had very low anticoagulant activity. Furthermore, they had a weak inactivating effect on virions in a virucidal assay at concentrations in the range of 60-100 μg/ml. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide, which had 0.4 sulfate group per monomer unit and an apparent molecular mass of 160 kDa, contained a backbone of α-(1 → 3)-linked d-mannopyranosyl residues substituted at C-6, C-4 and C-2 with single stub of β-d-xylopyranosyl residues. Sulfate groups, when present, are located at C-4 of α-(1 → 3)-linked d-mannopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer.Fil: Mandal, Pinaki. University of Burdwan; IndiaFil: Pujol, Carlos Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carlucci, Maria Josefina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chattopadhyay, Kausik. University of Burdwan; IndiaFil: Damonte, Elsa Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ray, Bimalendu. University of Burdwan; IndiaPergamon-Elsevier Science Ltd2008-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/74923Mandal, Pinaki; Pujol, Carlos Alberto; Carlucci, Maria Josefina; Chattopadhyay, Kausik; Damonte, Elsa Beatriz; et al.; Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei; Pergamon-Elsevier Science Ltd; Phytochemistry; 69; 11; 8-2008; 2193-21990031-9422CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.phytochem.2008.05.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0031942208002276info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:24Zoai:ri.conicet.gov.ar:11336/74923instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:24.709CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei
title Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei
spellingShingle Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei
Mandal, Pinaki
Antiviral Activity
Herpes Simplex Virus
Liagoraceae
Scinaia Hatei
Xylomannan Sulfate
title_short Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei
title_full Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei
title_fullStr Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei
title_full_unstemmed Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei
title_sort Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei
dc.creator.none.fl_str_mv Mandal, Pinaki
Pujol, Carlos Alberto
Carlucci, Maria Josefina
Chattopadhyay, Kausik
Damonte, Elsa Beatriz
Ray, Bimalendu
author Mandal, Pinaki
author_facet Mandal, Pinaki
Pujol, Carlos Alberto
Carlucci, Maria Josefina
Chattopadhyay, Kausik
Damonte, Elsa Beatriz
Ray, Bimalendu
author_role author
author2 Pujol, Carlos Alberto
Carlucci, Maria Josefina
Chattopadhyay, Kausik
Damonte, Elsa Beatriz
Ray, Bimalendu
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Antiviral Activity
Herpes Simplex Virus
Liagoraceae
Scinaia Hatei
Xylomannan Sulfate
topic Antiviral Activity
Herpes Simplex Virus
Liagoraceae
Scinaia Hatei
Xylomannan Sulfate
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study we have analyzed polysaccharide fractions isolated from Scinaia hatei. The crude water extract (ShWE) as well as one fraction (F1) obtained by size exclusion chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values ranging from 0.5 to 4.6 μg/ml were much lower than the cytotoxic concentration 50% (CC50) values (≥1000 μg/ml). These fractions had very low anticoagulant activity. Furthermore, they had a weak inactivating effect on virions in a virucidal assay at concentrations in the range of 60-100 μg/ml. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide, which had 0.4 sulfate group per monomer unit and an apparent molecular mass of 160 kDa, contained a backbone of α-(1 → 3)-linked d-mannopyranosyl residues substituted at C-6, C-4 and C-2 with single stub of β-d-xylopyranosyl residues. Sulfate groups, when present, are located at C-4 of α-(1 → 3)-linked d-mannopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer.
Fil: Mandal, Pinaki. University of Burdwan; India
Fil: Pujol, Carlos Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carlucci, Maria Josefina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chattopadhyay, Kausik. University of Burdwan; India
Fil: Damonte, Elsa Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ray, Bimalendu. University of Burdwan; India
description Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study we have analyzed polysaccharide fractions isolated from Scinaia hatei. The crude water extract (ShWE) as well as one fraction (F1) obtained by size exclusion chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values ranging from 0.5 to 4.6 μg/ml were much lower than the cytotoxic concentration 50% (CC50) values (≥1000 μg/ml). These fractions had very low anticoagulant activity. Furthermore, they had a weak inactivating effect on virions in a virucidal assay at concentrations in the range of 60-100 μg/ml. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide, which had 0.4 sulfate group per monomer unit and an apparent molecular mass of 160 kDa, contained a backbone of α-(1 → 3)-linked d-mannopyranosyl residues substituted at C-6, C-4 and C-2 with single stub of β-d-xylopyranosyl residues. Sulfate groups, when present, are located at C-4 of α-(1 → 3)-linked d-mannopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer.
publishDate 2008
dc.date.none.fl_str_mv 2008-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/74923
Mandal, Pinaki; Pujol, Carlos Alberto; Carlucci, Maria Josefina; Chattopadhyay, Kausik; Damonte, Elsa Beatriz; et al.; Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei; Pergamon-Elsevier Science Ltd; Phytochemistry; 69; 11; 8-2008; 2193-2199
0031-9422
CONICET Digital
CONICET
url http://hdl.handle.net/11336/74923
identifier_str_mv Mandal, Pinaki; Pujol, Carlos Alberto; Carlucci, Maria Josefina; Chattopadhyay, Kausik; Damonte, Elsa Beatriz; et al.; Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei; Pergamon-Elsevier Science Ltd; Phytochemistry; 69; 11; 8-2008; 2193-2199
0031-9422
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phytochem.2008.05.004
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0031942208002276
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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