Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica

Autores
Mandal, Pinaki; Mateu, Cecilia Gabriela; Chattopadhyay, Kausik; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; Ray, Bimalendu
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Natural compounds offer interesting pharmacological perspectives for antiviral drug development. In this study, we have analysed sulphated-fucan-containing fractions isolated from the brown seaweed Cystoseira indica. The crude water extract (CiWE) and the main fraction (CiF3) obtained by anion exchange chromatography had potent antiviral activity against herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) without cytotoxicity for Vero cell cultures. Furthermore, they had no direct inactivating effect on virions in a virucidal assay, and lacked anticoagulant activity. The mode of action of these compounds could be mainly ascribed to an inhibitory effect on virus adsorption. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide had an apparent molecular mass of 35 kDa and contained a backbone of α-(1→3)-linked fucopyranosyl residues substituted at C-2 with fucopyranosyl and xylopyranosyl residues. This sulphated fucan, considered the active principle of the C. indica water extract, also contained variously linked xylose and galactose units and glucuronic acid residues. Sulphate groups, if present, are located mostly at C-4 of (1→3)-linked fucopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer.
Fil: Mandal, Pinaki. The University Of Burdwan; India
Fil: Mateu, Cecilia Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chattopadhyay, Kausik. The University Of Burdwan; India
Fil: Pujol, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Damonte, Elsa Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ray, Bimalendu. The University Of Burdwan; India
Materia
ANTIVIRAL
BROWN SEAWEED
CYSTOSEIRA INDICA
HERPES SIMPLEX VIRUS
SULPHATED FUCAN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96084
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96084
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indicaMandal, PinakiMateu, Cecilia GabrielaChattopadhyay, KausikPujol, Carlos AlbertoDamonte, Elsa BeatrizRay, BimalenduANTIVIRALBROWN SEAWEEDCYSTOSEIRA INDICAHERPES SIMPLEX VIRUSSULPHATED FUCANhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Natural compounds offer interesting pharmacological perspectives for antiviral drug development. In this study, we have analysed sulphated-fucan-containing fractions isolated from the brown seaweed Cystoseira indica. The crude water extract (CiWE) and the main fraction (CiF3) obtained by anion exchange chromatography had potent antiviral activity against herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) without cytotoxicity for Vero cell cultures. Furthermore, they had no direct inactivating effect on virions in a virucidal assay, and lacked anticoagulant activity. The mode of action of these compounds could be mainly ascribed to an inhibitory effect on virus adsorption. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide had an apparent molecular mass of 35 kDa and contained a backbone of α-(1→3)-linked fucopyranosyl residues substituted at C-2 with fucopyranosyl and xylopyranosyl residues. This sulphated fucan, considered the active principle of the C. indica water extract, also contained variously linked xylose and galactose units and glucuronic acid residues. Sulphate groups, if present, are located mostly at C-4 of (1→3)-linked fucopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer.Fil: Mandal, Pinaki. The University Of Burdwan; IndiaFil: Mateu, Cecilia Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chattopadhyay, Kausik. The University Of Burdwan; IndiaFil: Pujol, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Damonte, Elsa Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ray, Bimalendu. The University Of Burdwan; IndiaInternational Medical Press2007-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96084Mandal, Pinaki; Mateu, Cecilia Gabriela; Chattopadhyay, Kausik; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; et al.; Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica; International Medical Press; Antiviral Chemistry & Chemotherapy; 18; 3; 6-2007; 153-1620956-3202CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/095632020701800305info:eu-repo/semantics/altIdentifier/doi/10.1177/095632020701800305info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:21Zoai:ri.conicet.gov.ar:11336/96084instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:22.133CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica
title Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica
spellingShingle Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica
Mandal, Pinaki
ANTIVIRAL
BROWN SEAWEED
CYSTOSEIRA INDICA
HERPES SIMPLEX VIRUS
SULPHATED FUCAN
title_short Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica
title_full Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica
title_fullStr Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica
title_full_unstemmed Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica
title_sort Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica
dc.creator.none.fl_str_mv Mandal, Pinaki
Mateu, Cecilia Gabriela
Chattopadhyay, Kausik
Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Ray, Bimalendu
author Mandal, Pinaki
author_facet Mandal, Pinaki
Mateu, Cecilia Gabriela
Chattopadhyay, Kausik
Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Ray, Bimalendu
author_role author
author2 Mateu, Cecilia Gabriela
Chattopadhyay, Kausik
Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Ray, Bimalendu
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANTIVIRAL
BROWN SEAWEED
CYSTOSEIRA INDICA
HERPES SIMPLEX VIRUS
SULPHATED FUCAN
topic ANTIVIRAL
BROWN SEAWEED
CYSTOSEIRA INDICA
HERPES SIMPLEX VIRUS
SULPHATED FUCAN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Natural compounds offer interesting pharmacological perspectives for antiviral drug development. In this study, we have analysed sulphated-fucan-containing fractions isolated from the brown seaweed Cystoseira indica. The crude water extract (CiWE) and the main fraction (CiF3) obtained by anion exchange chromatography had potent antiviral activity against herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) without cytotoxicity for Vero cell cultures. Furthermore, they had no direct inactivating effect on virions in a virucidal assay, and lacked anticoagulant activity. The mode of action of these compounds could be mainly ascribed to an inhibitory effect on virus adsorption. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide had an apparent molecular mass of 35 kDa and contained a backbone of α-(1→3)-linked fucopyranosyl residues substituted at C-2 with fucopyranosyl and xylopyranosyl residues. This sulphated fucan, considered the active principle of the C. indica water extract, also contained variously linked xylose and galactose units and glucuronic acid residues. Sulphate groups, if present, are located mostly at C-4 of (1→3)-linked fucopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer.
Fil: Mandal, Pinaki. The University Of Burdwan; India
Fil: Mateu, Cecilia Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chattopadhyay, Kausik. The University Of Burdwan; India
Fil: Pujol, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Damonte, Elsa Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ray, Bimalendu. The University Of Burdwan; India
description Natural compounds offer interesting pharmacological perspectives for antiviral drug development. In this study, we have analysed sulphated-fucan-containing fractions isolated from the brown seaweed Cystoseira indica. The crude water extract (CiWE) and the main fraction (CiF3) obtained by anion exchange chromatography had potent antiviral activity against herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) without cytotoxicity for Vero cell cultures. Furthermore, they had no direct inactivating effect on virions in a virucidal assay, and lacked anticoagulant activity. The mode of action of these compounds could be mainly ascribed to an inhibitory effect on virus adsorption. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide had an apparent molecular mass of 35 kDa and contained a backbone of α-(1→3)-linked fucopyranosyl residues substituted at C-2 with fucopyranosyl and xylopyranosyl residues. This sulphated fucan, considered the active principle of the C. indica water extract, also contained variously linked xylose and galactose units and glucuronic acid residues. Sulphate groups, if present, are located mostly at C-4 of (1→3)-linked fucopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer.
publishDate 2007
dc.date.none.fl_str_mv 2007-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96084
Mandal, Pinaki; Mateu, Cecilia Gabriela; Chattopadhyay, Kausik; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; et al.; Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica; International Medical Press; Antiviral Chemistry & Chemotherapy; 18; 3; 6-2007; 153-162
0956-3202
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96084
identifier_str_mv Mandal, Pinaki; Mateu, Cecilia Gabriela; Chattopadhyay, Kausik; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; et al.; Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica; International Medical Press; Antiviral Chemistry & Chemotherapy; 18; 3; 6-2007; 153-162
0956-3202
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/095632020701800305
info:eu-repo/semantics/altIdentifier/doi/10.1177/095632020701800305
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Medical Press
publisher.none.fl_str_mv International Medical Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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