Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification i...

Autores
Fernandez Alfaya, Jose Elias; López Márquez, Violeta; García Jimenez, Ricardo; Fernández Álvarez, Fernando Ángel; Bigatti, Gregorio; Machordom Barbe, Annie
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microsatellite loci (26 in total) were isolated for the first time for the entocommensal nemertean Malacobdella arrokeana, using 454 GS-FLX Titanium pyrosequencing. We developed conditions for amplifying these markers in 8 multiplex and 5 individual reactions. One to 14 alleles were detected per locus across 25 samples analyzed from San Matías Gulf, Patagonia (Argentina). For the 25 polymorphic loci, observed and expected heterozygosities ranged from 0.125 to 0.920 and 0.119 to 0.890, respectively; five loci deviated from Hardy–Weinberg equilibrium. Limited distribution and host specificity of M.arrokeana, which only inhabits Panopea abbreviata, have endangered this nemertean given the unregulated commercial exploitation of its host. These useful markers provide data for future conservation strategies. Cross-amplifications were also tested in 34 nemerteans species, representing the major lineages in this phylum. In total, 18 of the 26 loci (from one to 11 per species) gave clear allelic profiles.
Fil: Fernandez Alfaya, Jose Elias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: López Márquez, Violeta. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
Fil: García Jimenez, Ricardo. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
Fil: Fernández Álvarez, Fernando Ángel. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
Fil: Bigatti, Gregorio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Machordom Barbe, Annie. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
Materia
454 Pyrosequencing
Nemertean
Patagonia
Population Structure
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36681
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean speciesFernandez Alfaya, Jose EliasLópez Márquez, VioletaGarcía Jimenez, RicardoFernández Álvarez, Fernando ÁngelBigatti, GregorioMachordom Barbe, Annie454 PyrosequencingNemerteanPatagoniaPopulation Structurehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Microsatellite loci (26 in total) were isolated for the first time for the entocommensal nemertean Malacobdella arrokeana, using 454 GS-FLX Titanium pyrosequencing. We developed conditions for amplifying these markers in 8 multiplex and 5 individual reactions. One to 14 alleles were detected per locus across 25 samples analyzed from San Matías Gulf, Patagonia (Argentina). For the 25 polymorphic loci, observed and expected heterozygosities ranged from 0.125 to 0.920 and 0.119 to 0.890, respectively; five loci deviated from Hardy–Weinberg equilibrium. Limited distribution and host specificity of M.arrokeana, which only inhabits Panopea abbreviata, have endangered this nemertean given the unregulated commercial exploitation of its host. These useful markers provide data for future conservation strategies. Cross-amplifications were also tested in 34 nemerteans species, representing the major lineages in this phylum. In total, 18 of the 26 loci (from one to 11 per species) gave clear allelic profiles.Fil: Fernandez Alfaya, Jose Elias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: López Márquez, Violeta. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; EspañaFil: García Jimenez, Ricardo. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; EspañaFil: Fernández Álvarez, Fernando Ángel. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; EspañaFil: Bigatti, Gregorio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Machordom Barbe, Annie. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; EspañaSpringer2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36681Fernandez Alfaya, Jose Elias; López Márquez, Violeta; García Jimenez, Ricardo; Fernández Álvarez, Fernando Ángel; Bigatti, Gregorio; et al.; Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species; Springer; Conservation Genetics Resources; 6; 1; 3-2014; 147-1501877-72521877-7260CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12686-013-0029-zinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12686-013-0029-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:29Zoai:ri.conicet.gov.ar:11336/36681instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:30.197CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species
title Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species
spellingShingle Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species
Fernandez Alfaya, Jose Elias
454 Pyrosequencing
Nemertean
Patagonia
Population Structure
title_short Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species
title_full Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species
title_fullStr Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species
title_full_unstemmed Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species
title_sort Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species
dc.creator.none.fl_str_mv Fernandez Alfaya, Jose Elias
López Márquez, Violeta
García Jimenez, Ricardo
Fernández Álvarez, Fernando Ángel
Bigatti, Gregorio
Machordom Barbe, Annie
author Fernandez Alfaya, Jose Elias
author_facet Fernandez Alfaya, Jose Elias
López Márquez, Violeta
García Jimenez, Ricardo
Fernández Álvarez, Fernando Ángel
Bigatti, Gregorio
Machordom Barbe, Annie
author_role author
author2 López Márquez, Violeta
García Jimenez, Ricardo
Fernández Álvarez, Fernando Ángel
Bigatti, Gregorio
Machordom Barbe, Annie
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv 454 Pyrosequencing
Nemertean
Patagonia
Population Structure
topic 454 Pyrosequencing
Nemertean
Patagonia
Population Structure
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microsatellite loci (26 in total) were isolated for the first time for the entocommensal nemertean Malacobdella arrokeana, using 454 GS-FLX Titanium pyrosequencing. We developed conditions for amplifying these markers in 8 multiplex and 5 individual reactions. One to 14 alleles were detected per locus across 25 samples analyzed from San Matías Gulf, Patagonia (Argentina). For the 25 polymorphic loci, observed and expected heterozygosities ranged from 0.125 to 0.920 and 0.119 to 0.890, respectively; five loci deviated from Hardy–Weinberg equilibrium. Limited distribution and host specificity of M.arrokeana, which only inhabits Panopea abbreviata, have endangered this nemertean given the unregulated commercial exploitation of its host. These useful markers provide data for future conservation strategies. Cross-amplifications were also tested in 34 nemerteans species, representing the major lineages in this phylum. In total, 18 of the 26 loci (from one to 11 per species) gave clear allelic profiles.
Fil: Fernandez Alfaya, Jose Elias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: López Márquez, Violeta. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
Fil: García Jimenez, Ricardo. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
Fil: Fernández Álvarez, Fernando Ángel. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
Fil: Bigatti, Gregorio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Machordom Barbe, Annie. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
description Microsatellite loci (26 in total) were isolated for the first time for the entocommensal nemertean Malacobdella arrokeana, using 454 GS-FLX Titanium pyrosequencing. We developed conditions for amplifying these markers in 8 multiplex and 5 individual reactions. One to 14 alleles were detected per locus across 25 samples analyzed from San Matías Gulf, Patagonia (Argentina). For the 25 polymorphic loci, observed and expected heterozygosities ranged from 0.125 to 0.920 and 0.119 to 0.890, respectively; five loci deviated from Hardy–Weinberg equilibrium. Limited distribution and host specificity of M.arrokeana, which only inhabits Panopea abbreviata, have endangered this nemertean given the unregulated commercial exploitation of its host. These useful markers provide data for future conservation strategies. Cross-amplifications were also tested in 34 nemerteans species, representing the major lineages in this phylum. In total, 18 of the 26 loci (from one to 11 per species) gave clear allelic profiles.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36681
Fernandez Alfaya, Jose Elias; López Márquez, Violeta; García Jimenez, Ricardo; Fernández Álvarez, Fernando Ángel; Bigatti, Gregorio; et al.; Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species; Springer; Conservation Genetics Resources; 6; 1; 3-2014; 147-150
1877-7252
1877-7260
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36681
identifier_str_mv Fernandez Alfaya, Jose Elias; López Márquez, Violeta; García Jimenez, Ricardo; Fernández Álvarez, Fernando Ángel; Bigatti, Gregorio; et al.; Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species; Springer; Conservation Genetics Resources; 6; 1; 3-2014; 147-150
1877-7252
1877-7260
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s12686-013-0029-z
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12686-013-0029-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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