Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial su...

Autores: Indo, Yuhki; Kitahara, Shugo; Tomokiyo, Mikado; Araki, Shota; Islam, Md Aminul; Zhou, Binghui; Albarracín, Leonardo Miguel; Miyazaki, Ayako; Ikeda Ohtsubo, Wakako; Nochi, Tomonori; Takenouchi, Takato; Uenishi, Hirohide; Aso, Hisashi; Takahashi, Hideki; Kurata, Shoichiro; Villena, Julio Cesar; Kitazawa, Haruki
Año de publicación: 2021
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Previously, we constructed a library of Ligilactobacillus salivarius strains from the intestine of wakame-fed pigs and reported a strain-dependent capacity to modulate IFN-β expression in porcine intestinal epithelial (PIE) cells. In this work, we further characterized the immunomodulatory activities of L. salivarius strains from wakame-fed pigs by evaluating their ability to modulate TLR3- and TLR4-mediated innate immune responses in PIE cells. Two strains with a remarkable immunomodulatory potential were selected: L. salivarius FFIG35 and FFIG58. Both strains improved IFN-β, IFN-λ and antiviral factors expression in PIE cells after TLR3 activation, which correlated with an enhanced resistance to rotavirus infection. Moreover, a model of enterotoxigenic E. coli (ETEC)/rotavirus superinfection in PIE cells was developed. Cells were more susceptible to rotavirus infection when the challenge occurred in conjunction with ETEC compared to the virus alone. However, L. salivarius FFIG35 and FFIG58 maintained their ability to enhance IFN-β, IFN-λ and antiviral factors expression in PIE cells, and to reduce rotavirus replication in the context of superinfection. We also demonstrated that FFIG35 and FFIG58 strains regulated the immune response of PIE cells to rotavirus challenge or ETEC/rotavirus superinfection through the modulation of negative regulators of the TLR signaling pathway. In vivo studies performed in mice models confirmed the ability of L. salivarius FFIG58 to beneficially modulate the innate immune response and protect against ETEC infection. The results of this work contribute to the understanding of beneficial lactobacilli interactions with epithelial cells and allow us to hypothesize that the FFIG35 or FFIG58 strains could be used for the development of highly efficient functional feed to improve immune health status and reduce the severity of intestinal infections and superinfections in weaned piglets.
Fil: Indo, Yuhki. Tohoku University; Japón
Fil: Kitahara, Shugo. Tohoku University; Japón
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Araki, Shota. Tohoku University; Japón
Fil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina
Fil: Miyazaki, Ayako. National Institute of Animal Health; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Nochi, Tomonori. Tohoku University; Japón
Fil: Takenouchi, Takato. National Agriculture And Food Research Organization; Japón
Fil: Uenishi, Hirohide. National Agriculture and Food Research Organization; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Takahashi, Hideki. Tohoku University; Japón
Fil: Kurata, Shoichiro. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia: INNATE IMMUNITY
INTESTINAL SUPERINFECTION
LACTOBACILLI
PORCINE INTESTINAL EPITHELIAL CELLS
ROTAVIRUS INFECTION
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/153877

Acceder

id	CONICETDig_23142e11f9c815e7a8e462bf5c14fc2b
oai_identifier_str	oai:ri.conicet.gov.ar:11336/153877
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfectionIndo, YuhkiKitahara, ShugoTomokiyo, MikadoAraki, ShotaIslam, Md AminulZhou, BinghuiAlbarracín, Leonardo MiguelMiyazaki, AyakoIkeda Ohtsubo, WakakoNochi, TomonoriTakenouchi, TakatoUenishi, HirohideAso, HisashiTakahashi, HidekiKurata, ShoichiroVillena, Julio CesarKitazawa, HarukiINNATE IMMUNITYINTESTINAL SUPERINFECTIONLACTOBACILLIPORCINE INTESTINAL EPITHELIAL CELLSROTAVIRUS INFECTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Previously, we constructed a library of Ligilactobacillus salivarius strains from the intestine of wakame-fed pigs and reported a strain-dependent capacity to modulate IFN-β expression in porcine intestinal epithelial (PIE) cells. In this work, we further characterized the immunomodulatory activities of L. salivarius strains from wakame-fed pigs by evaluating their ability to modulate TLR3- and TLR4-mediated innate immune responses in PIE cells. Two strains with a remarkable immunomodulatory potential were selected: L. salivarius FFIG35 and FFIG58. Both strains improved IFN-β, IFN-λ and antiviral factors expression in PIE cells after TLR3 activation, which correlated with an enhanced resistance to rotavirus infection. Moreover, a model of enterotoxigenic E. coli (ETEC)/rotavirus superinfection in PIE cells was developed. Cells were more susceptible to rotavirus infection when the challenge occurred in conjunction with ETEC compared to the virus alone. However, L. salivarius FFIG35 and FFIG58 maintained their ability to enhance IFN-β, IFN-λ and antiviral factors expression in PIE cells, and to reduce rotavirus replication in the context of superinfection. We also demonstrated that FFIG35 and FFIG58 strains regulated the immune response of PIE cells to rotavirus challenge or ETEC/rotavirus superinfection through the modulation of negative regulators of the TLR signaling pathway. In vivo studies performed in mice models confirmed the ability of L. salivarius FFIG58 to beneficially modulate the innate immune response and protect against ETEC infection. The results of this work contribute to the understanding of beneficial lactobacilli interactions with epithelial cells and allow us to hypothesize that the FFIG35 or FFIG58 strains could be used for the development of highly efficient functional feed to improve immune health status and reduce the severity of intestinal infections and superinfections in weaned piglets.Fil: Indo, Yuhki. Tohoku University; JapónFil: Kitahara, Shugo. Tohoku University; JapónFil: Tomokiyo, Mikado. Tohoku University; JapónFil: Araki, Shota. Tohoku University; JapónFil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; BangladeshFil: Zhou, Binghui. Tohoku University; JapónFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; ArgentinaFil: Miyazaki, Ayako. National Institute of Animal Health; JapónFil: Ikeda Ohtsubo, Wakako. Tohoku University; JapónFil: Nochi, Tomonori. Tohoku University; JapónFil: Takenouchi, Takato. National Agriculture And Food Research Organization; JapónFil: Uenishi, Hirohide. National Agriculture and Food Research Organization; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Takahashi, Hideki. Tohoku University; JapónFil: Kurata, Shoichiro. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Kitazawa, Haruki. Tohoku University; JapónFrontiers Media2021-06-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153877Indo, Yuhki; Kitahara, Shugo; Tomokiyo, Mikado; Araki, Shota; Islam, Md Aminul; et al.; Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfection; Frontiers Media; Frontiers in Immunology; 12; 7-6-2021; 1-221664-32241664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2021.652923info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2021.652923/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:21Zoai:ri.conicet.gov.ar:11336/153877instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:21.389CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfection
title	Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfection
spellingShingle	Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfection Indo, Yuhki INNATE IMMUNITY INTESTINAL SUPERINFECTION LACTOBACILLI PORCINE INTESTINAL EPITHELIAL CELLS ROTAVIRUS INFECTION
title_short	Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfection
title_full	Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfection
title_fullStr	Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfection
title_full_unstemmed	Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfection
title_sort	Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfection
dc.creator.none.fl_str_mv	Indo, Yuhki Kitahara, Shugo Tomokiyo, Mikado Araki, Shota Islam, Md Aminul Zhou, Binghui Albarracín, Leonardo Miguel Miyazaki, Ayako Ikeda Ohtsubo, Wakako Nochi, Tomonori Takenouchi, Takato Uenishi, Hirohide Aso, Hisashi Takahashi, Hideki Kurata, Shoichiro Villena, Julio Cesar Kitazawa, Haruki
author	Indo, Yuhki
author_facet	Indo, Yuhki Kitahara, Shugo Tomokiyo, Mikado Araki, Shota Islam, Md Aminul Zhou, Binghui Albarracín, Leonardo Miguel Miyazaki, Ayako Ikeda Ohtsubo, Wakako Nochi, Tomonori Takenouchi, Takato Uenishi, Hirohide Aso, Hisashi Takahashi, Hideki Kurata, Shoichiro Villena, Julio Cesar Kitazawa, Haruki
author_role	author
author2	Kitahara, Shugo Tomokiyo, Mikado Araki, Shota Islam, Md Aminul Zhou, Binghui Albarracín, Leonardo Miguel Miyazaki, Ayako Ikeda Ohtsubo, Wakako Nochi, Tomonori Takenouchi, Takato Uenishi, Hirohide Aso, Hisashi Takahashi, Hideki Kurata, Shoichiro Villena, Julio Cesar Kitazawa, Haruki
author2_role	author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	INNATE IMMUNITY INTESTINAL SUPERINFECTION LACTOBACILLI PORCINE INTESTINAL EPITHELIAL CELLS ROTAVIRUS INFECTION
topic	INNATE IMMUNITY INTESTINAL SUPERINFECTION LACTOBACILLI PORCINE INTESTINAL EPITHELIAL CELLS ROTAVIRUS INFECTION
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv	Previously, we constructed a library of Ligilactobacillus salivarius strains from the intestine of wakame-fed pigs and reported a strain-dependent capacity to modulate IFN-β expression in porcine intestinal epithelial (PIE) cells. In this work, we further characterized the immunomodulatory activities of L. salivarius strains from wakame-fed pigs by evaluating their ability to modulate TLR3- and TLR4-mediated innate immune responses in PIE cells. Two strains with a remarkable immunomodulatory potential were selected: L. salivarius FFIG35 and FFIG58. Both strains improved IFN-β, IFN-λ and antiviral factors expression in PIE cells after TLR3 activation, which correlated with an enhanced resistance to rotavirus infection. Moreover, a model of enterotoxigenic E. coli (ETEC)/rotavirus superinfection in PIE cells was developed. Cells were more susceptible to rotavirus infection when the challenge occurred in conjunction with ETEC compared to the virus alone. However, L. salivarius FFIG35 and FFIG58 maintained their ability to enhance IFN-β, IFN-λ and antiviral factors expression in PIE cells, and to reduce rotavirus replication in the context of superinfection. We also demonstrated that FFIG35 and FFIG58 strains regulated the immune response of PIE cells to rotavirus challenge or ETEC/rotavirus superinfection through the modulation of negative regulators of the TLR signaling pathway. In vivo studies performed in mice models confirmed the ability of L. salivarius FFIG58 to beneficially modulate the innate immune response and protect against ETEC infection. The results of this work contribute to the understanding of beneficial lactobacilli interactions with epithelial cells and allow us to hypothesize that the FFIG35 or FFIG58 strains could be used for the development of highly efficient functional feed to improve immune health status and reduce the severity of intestinal infections and superinfections in weaned piglets. Fil: Indo, Yuhki. Tohoku University; Japón Fil: Kitahara, Shugo. Tohoku University; Japón Fil: Tomokiyo, Mikado. Tohoku University; Japón Fil: Araki, Shota. Tohoku University; Japón Fil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh Fil: Zhou, Binghui. Tohoku University; Japón Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina Fil: Miyazaki, Ayako. National Institute of Animal Health; Japón Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón Fil: Nochi, Tomonori. Tohoku University; Japón Fil: Takenouchi, Takato. National Agriculture And Food Research Organization; Japón Fil: Uenishi, Hirohide. National Agriculture and Food Research Organization; Japón Fil: Aso, Hisashi. Tohoku University; Japón Fil: Takahashi, Hideki. Tohoku University; Japón Fil: Kurata, Shoichiro. Tohoku University; Japón Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Kitazawa, Haruki. Tohoku University; Japón
description	Previously, we constructed a library of Ligilactobacillus salivarius strains from the intestine of wakame-fed pigs and reported a strain-dependent capacity to modulate IFN-β expression in porcine intestinal epithelial (PIE) cells. In this work, we further characterized the immunomodulatory activities of L. salivarius strains from wakame-fed pigs by evaluating their ability to modulate TLR3- and TLR4-mediated innate immune responses in PIE cells. Two strains with a remarkable immunomodulatory potential were selected: L. salivarius FFIG35 and FFIG58. Both strains improved IFN-β, IFN-λ and antiviral factors expression in PIE cells after TLR3 activation, which correlated with an enhanced resistance to rotavirus infection. Moreover, a model of enterotoxigenic E. coli (ETEC)/rotavirus superinfection in PIE cells was developed. Cells were more susceptible to rotavirus infection when the challenge occurred in conjunction with ETEC compared to the virus alone. However, L. salivarius FFIG35 and FFIG58 maintained their ability to enhance IFN-β, IFN-λ and antiviral factors expression in PIE cells, and to reduce rotavirus replication in the context of superinfection. We also demonstrated that FFIG35 and FFIG58 strains regulated the immune response of PIE cells to rotavirus challenge or ETEC/rotavirus superinfection through the modulation of negative regulators of the TLR signaling pathway. In vivo studies performed in mice models confirmed the ability of L. salivarius FFIG58 to beneficially modulate the innate immune response and protect against ETEC infection. The results of this work contribute to the understanding of beneficial lactobacilli interactions with epithelial cells and allow us to hypothesize that the FFIG35 or FFIG58 strains could be used for the development of highly efficient functional feed to improve immune health status and reduce the severity of intestinal infections and superinfections in weaned piglets.
publishDate	2021
dc.date.none.fl_str_mv	2021-06-07
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/153877 Indo, Yuhki; Kitahara, Shugo; Tomokiyo, Mikado; Araki, Shota; Islam, Md Aminul; et al.; Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfection; Frontiers Media; Frontiers in Immunology; 12; 7-6-2021; 1-22 1664-3224 1664-3224 CONICET Digital CONICET
url	http://hdl.handle.net/11336/153877
identifier_str_mv	Indo, Yuhki; Kitahara, Shugo; Tomokiyo, Mikado; Araki, Shota; Islam, Md Aminul; et al.; Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial superinfection; Frontiers Media; Frontiers in Immunology; 12; 7-6-2021; 1-22 1664-3224 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2021.652923 info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2021.652923/
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Frontiers Media
publisher.none.fl_str_mv	Frontiers Media
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1844613211860500480
score	13.070432

Ligilactobacillus salivarius strains isolated rrom the porcine gut modulate innate immune responses in epithelial cells and improve protection against intestinal viral-bacterial su...

Publicaciones similares