Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.

Autores
Castillo, Natalia Alejandra; Maldonado Galdeano, María Carolina; de Moreno, Maria Alejandra; Perdigon, Gabriela del Valle
Año de publicación
2011
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Lactobacillus casei CRL431 (Lc) protected against Salmonella enterica serovar Typhimurium (ST) infection modulating the immune cells in a mouse model. Intestinal epithelial cells (IECs) participate in the early response against ST. Our aim was to evaluate IEC response in mice received Lc and infected with ST, and using vitro assays. Lc7d-group received Lc during 7days (d), was challenged with ST and continued Lc administration 10d post-infection (PI). IECs were isolated from these mice and from untreated (UC) and infected (IC) controls. IECs from UC were also challenged with ST or Lc for in vitro tests. IL-6 and MCP-1 increased significantly in the IEC culture supernatants from Lc7d-group compared to UC (before infection) and to IC (24h PI). IC increased the release of IL-6 from IECs, 7 and 10d PI. In vitro, IECs challenged with Lc released higher levels of IL-6 than IECs challenged with ST. These results showed another font of IL-6, necessary for B-cell clonal expansion and specific anti-ST IgA-s production, both increased in mice from Lc7d-group. The participation of cells from innate response, such as macrophages, in Lc stimulation agrees with the results obtained for MCP-1. Instead, ST infection increased neutrophils influx and mieloperoxidase activity in the intestine, which decreased in Lc7d-group. Lc administration induced an earlier response against ST, increased IL-6 release and MCP-1 secretion for macrophage recruitment and limited the influx of neutrophils. Lc stimulates not only the intestinal immune cells, but also IECs, first step of this infection.
Fil: Castillo, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología. Cátedra de Inmunología; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
15th International Congress of Mucosal Immunology
Paris
Francia
Universidad de París Descartes
Materia
PROBIOTIC
INTESTINAL INFECTION
SALMONELLA
INTESTINAL EPITHELIAL CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194941
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/194941
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.Castillo, Natalia AlejandraMaldonado Galdeano, María Carolinade Moreno, Maria AlejandraPerdigon, Gabriela del VallePROBIOTICINTESTINAL INFECTIONSALMONELLAINTESTINAL EPITHELIAL CELLShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Lactobacillus casei CRL431 (Lc) protected against Salmonella enterica serovar Typhimurium (ST) infection modulating the immune cells in a mouse model. Intestinal epithelial cells (IECs) participate in the early response against ST. Our aim was to evaluate IEC response in mice received Lc and infected with ST, and using vitro assays. Lc7d-group received Lc during 7days (d), was challenged with ST and continued Lc administration 10d post-infection (PI). IECs were isolated from these mice and from untreated (UC) and infected (IC) controls. IECs from UC were also challenged with ST or Lc for in vitro tests. IL-6 and MCP-1 increased significantly in the IEC culture supernatants from Lc7d-group compared to UC (before infection) and to IC (24h PI). IC increased the release of IL-6 from IECs, 7 and 10d PI. In vitro, IECs challenged with Lc released higher levels of IL-6 than IECs challenged with ST. These results showed another font of IL-6, necessary for B-cell clonal expansion and specific anti-ST IgA-s production, both increased in mice from Lc7d-group. The participation of cells from innate response, such as macrophages, in Lc stimulation agrees with the results obtained for MCP-1. Instead, ST infection increased neutrophils influx and mieloperoxidase activity in the intestine, which decreased in Lc7d-group. Lc administration induced an earlier response against ST, increased IL-6 release and MCP-1 secretion for macrophage recruitment and limited the influx of neutrophils. Lc stimulates not only the intestinal immune cells, but also IECs, first step of this infection.Fil: Castillo, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología. Cátedra de Inmunología; ArgentinaFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina15th International Congress of Mucosal ImmunologyParisFranciaUniversidad de París DescartesUniversidad de París Descartes2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194941Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.; 15th International Congress of Mucosal Immunology; Paris; Francia; 2011; 149-149CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.socmucimm.org/media/i0wjjgsp/abstract-supplement-chronological-final-6-29-11.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:00Zoai:ri.conicet.gov.ar:11336/194941instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:01.138CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.
title Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.
spellingShingle Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.
Castillo, Natalia Alejandra
PROBIOTIC
INTESTINAL INFECTION
SALMONELLA
INTESTINAL EPITHELIAL CELLS
title_short Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.
title_full Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.
title_fullStr Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.
title_full_unstemmed Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.
title_sort Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.
dc.creator.none.fl_str_mv Castillo, Natalia Alejandra
Maldonado Galdeano, María Carolina
de Moreno, Maria Alejandra
Perdigon, Gabriela del Valle
author Castillo, Natalia Alejandra
author_facet Castillo, Natalia Alejandra
Maldonado Galdeano, María Carolina
de Moreno, Maria Alejandra
Perdigon, Gabriela del Valle
author_role author
author2 Maldonado Galdeano, María Carolina
de Moreno, Maria Alejandra
Perdigon, Gabriela del Valle
author2_role author
author
author
dc.subject.none.fl_str_mv PROBIOTIC
INTESTINAL INFECTION
SALMONELLA
INTESTINAL EPITHELIAL CELLS
topic PROBIOTIC
INTESTINAL INFECTION
SALMONELLA
INTESTINAL EPITHELIAL CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Lactobacillus casei CRL431 (Lc) protected against Salmonella enterica serovar Typhimurium (ST) infection modulating the immune cells in a mouse model. Intestinal epithelial cells (IECs) participate in the early response against ST. Our aim was to evaluate IEC response in mice received Lc and infected with ST, and using vitro assays. Lc7d-group received Lc during 7days (d), was challenged with ST and continued Lc administration 10d post-infection (PI). IECs were isolated from these mice and from untreated (UC) and infected (IC) controls. IECs from UC were also challenged with ST or Lc for in vitro tests. IL-6 and MCP-1 increased significantly in the IEC culture supernatants from Lc7d-group compared to UC (before infection) and to IC (24h PI). IC increased the release of IL-6 from IECs, 7 and 10d PI. In vitro, IECs challenged with Lc released higher levels of IL-6 than IECs challenged with ST. These results showed another font of IL-6, necessary for B-cell clonal expansion and specific anti-ST IgA-s production, both increased in mice from Lc7d-group. The participation of cells from innate response, such as macrophages, in Lc stimulation agrees with the results obtained for MCP-1. Instead, ST infection increased neutrophils influx and mieloperoxidase activity in the intestine, which decreased in Lc7d-group. Lc administration induced an earlier response against ST, increased IL-6 release and MCP-1 secretion for macrophage recruitment and limited the influx of neutrophils. Lc stimulates not only the intestinal immune cells, but also IECs, first step of this infection.
Fil: Castillo, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología. Cátedra de Inmunología; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
15th International Congress of Mucosal Immunology
Paris
Francia
Universidad de París Descartes
description Lactobacillus casei CRL431 (Lc) protected against Salmonella enterica serovar Typhimurium (ST) infection modulating the immune cells in a mouse model. Intestinal epithelial cells (IECs) participate in the early response against ST. Our aim was to evaluate IEC response in mice received Lc and infected with ST, and using vitro assays. Lc7d-group received Lc during 7days (d), was challenged with ST and continued Lc administration 10d post-infection (PI). IECs were isolated from these mice and from untreated (UC) and infected (IC) controls. IECs from UC were also challenged with ST or Lc for in vitro tests. IL-6 and MCP-1 increased significantly in the IEC culture supernatants from Lc7d-group compared to UC (before infection) and to IC (24h PI). IC increased the release of IL-6 from IECs, 7 and 10d PI. In vitro, IECs challenged with Lc released higher levels of IL-6 than IECs challenged with ST. These results showed another font of IL-6, necessary for B-cell clonal expansion and specific anti-ST IgA-s production, both increased in mice from Lc7d-group. The participation of cells from innate response, such as macrophages, in Lc stimulation agrees with the results obtained for MCP-1. Instead, ST infection increased neutrophils influx and mieloperoxidase activity in the intestine, which decreased in Lc7d-group. Lc administration induced an earlier response against ST, increased IL-6 release and MCP-1 secretion for macrophage recruitment and limited the influx of neutrophils. Lc stimulates not only the intestinal immune cells, but also IECs, first step of this infection.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194941
Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.; 15th International Congress of Mucosal Immunology; Paris; Francia; 2011; 149-149
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194941
identifier_str_mv Improved response of intestinal epithelial cells against Salmonella enterica serovar Typhimurium infection in mice fed with Lactobacillus casei CRL 431.; 15th International Congress of Mucosal Immunology; Paris; Francia; 2011; 149-149
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.socmucimm.org/media/i0wjjgsp/abstract-supplement-chronological-final-6-29-11.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad de París Descartes
publisher.none.fl_str_mv Universidad de París Descartes
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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