Ecotoxicological assessment of soil microbial community tolerance to glyphosate

Autores
Allegrini, Marco; Zabaloy, Maria Celina; Gomez, Elena del V.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glyphosate is the most used herbicide worldwide. While contrasting results have been observed related with its impact on soil microbial communities, more studies are necessary to elucidate the potential effects of the herbicide. Differences in tolerance detected by Pollution Induced Community Tolerance (PICT) approach could reflect these effects. The objective of the present study was to assess the tolerance to glyphosate (the active ingredient and a commercial formulation) of contrasting soils with (H) and without (NH) history of exposure. The hypothesis of a higher tolerance in H soils due to a sustained selection pressure on community structure was tested through the PICT approach. Results indicated that tolerance to glyphosate is not consistent with previous history of exposure to the herbicide. Studies of community structure based on Denaturant Gradient Gel Electrophoresis (DGGE) and Quantitative Real Time PCR (Q-PCR) showed that composition and abundance of bacteria, respectively, did not differ significantly between H and NH soils, in accordance with PICT results. Consequently, the lack of significant differences in tolerance in most cases could be partially explained by a similar community structure. This is, to our knowledge, the first report of PICT approach with glyphosate examining tolerance at soil microbial community level. Future culture-independent analysis focused on specific groups of microorganisms instead of whole microbial communities could be important to provide stronger evidences supporting our results and conclusions.
Fil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Sistema de Producción Vegetal. Laboratorio Biodiversidad Vegetal y Microbiana Fitopatologia; Argentina
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina
Fil: Gomez, Elena del V.. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Sistema de Producción Vegetal. Laboratorio Biodiversidad Vegetal y Microbiana Fitopatologia; Argentina
Materia
Glyphosate
Soil Microbial Community
Pict Assay
Dgge Profiles
Q-Pcr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11585
Acceder
id CONICETDig_4c1346014e1c7330a8922828fed8fead
oai_identifier_str oai:ri.conicet.gov.ar:11336/11585
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ecotoxicological assessment of soil microbial community tolerance to glyphosateAllegrini, MarcoZabaloy, Maria CelinaGomez, Elena del V.GlyphosateSoil Microbial CommunityPict AssayDgge ProfilesQ-Pcrhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Glyphosate is the most used herbicide worldwide. While contrasting results have been observed related with its impact on soil microbial communities, more studies are necessary to elucidate the potential effects of the herbicide. Differences in tolerance detected by Pollution Induced Community Tolerance (PICT) approach could reflect these effects. The objective of the present study was to assess the tolerance to glyphosate (the active ingredient and a commercial formulation) of contrasting soils with (H) and without (NH) history of exposure. The hypothesis of a higher tolerance in H soils due to a sustained selection pressure on community structure was tested through the PICT approach. Results indicated that tolerance to glyphosate is not consistent with previous history of exposure to the herbicide. Studies of community structure based on Denaturant Gradient Gel Electrophoresis (DGGE) and Quantitative Real Time PCR (Q-PCR) showed that composition and abundance of bacteria, respectively, did not differ significantly between H and NH soils, in accordance with PICT results. Consequently, the lack of significant differences in tolerance in most cases could be partially explained by a similar community structure. This is, to our knowledge, the first report of PICT approach with glyphosate examining tolerance at soil microbial community level. Future culture-independent analysis focused on specific groups of microorganisms instead of whole microbial communities could be important to provide stronger evidences supporting our results and conclusions.Fil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Sistema de Producción Vegetal. Laboratorio Biodiversidad Vegetal y Microbiana Fitopatologia; ArgentinaFil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Gomez, Elena del V.. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Sistema de Producción Vegetal. Laboratorio Biodiversidad Vegetal y Microbiana Fitopatologia; ArgentinaElsevier Science2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11585Allegrini, Marco; Zabaloy, Maria Celina; Gomez, Elena del V.; Ecotoxicological assessment of soil microbial community tolerance to glyphosate; Elsevier Science; Science of the Total Environment; 553; 11-2015; 60-680048-9697enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0048969715302898info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2015.06.096info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:45Zoai:ri.conicet.gov.ar:11336/11585instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:45.506CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ecotoxicological assessment of soil microbial community tolerance to glyphosate
title Ecotoxicological assessment of soil microbial community tolerance to glyphosate
spellingShingle Ecotoxicological assessment of soil microbial community tolerance to glyphosate
Allegrini, Marco
Glyphosate
Soil Microbial Community
Pict Assay
Dgge Profiles
Q-Pcr
title_short Ecotoxicological assessment of soil microbial community tolerance to glyphosate
title_full Ecotoxicological assessment of soil microbial community tolerance to glyphosate
title_fullStr Ecotoxicological assessment of soil microbial community tolerance to glyphosate
title_full_unstemmed Ecotoxicological assessment of soil microbial community tolerance to glyphosate
title_sort Ecotoxicological assessment of soil microbial community tolerance to glyphosate
dc.creator.none.fl_str_mv Allegrini, Marco
Zabaloy, Maria Celina
Gomez, Elena del V.
author Allegrini, Marco
author_facet Allegrini, Marco
Zabaloy, Maria Celina
Gomez, Elena del V.
author_role author
author2 Zabaloy, Maria Celina
Gomez, Elena del V.
author2_role author
author
dc.subject.none.fl_str_mv Glyphosate
Soil Microbial Community
Pict Assay
Dgge Profiles
Q-Pcr
topic Glyphosate
Soil Microbial Community
Pict Assay
Dgge Profiles
Q-Pcr
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Glyphosate is the most used herbicide worldwide. While contrasting results have been observed related with its impact on soil microbial communities, more studies are necessary to elucidate the potential effects of the herbicide. Differences in tolerance detected by Pollution Induced Community Tolerance (PICT) approach could reflect these effects. The objective of the present study was to assess the tolerance to glyphosate (the active ingredient and a commercial formulation) of contrasting soils with (H) and without (NH) history of exposure. The hypothesis of a higher tolerance in H soils due to a sustained selection pressure on community structure was tested through the PICT approach. Results indicated that tolerance to glyphosate is not consistent with previous history of exposure to the herbicide. Studies of community structure based on Denaturant Gradient Gel Electrophoresis (DGGE) and Quantitative Real Time PCR (Q-PCR) showed that composition and abundance of bacteria, respectively, did not differ significantly between H and NH soils, in accordance with PICT results. Consequently, the lack of significant differences in tolerance in most cases could be partially explained by a similar community structure. This is, to our knowledge, the first report of PICT approach with glyphosate examining tolerance at soil microbial community level. Future culture-independent analysis focused on specific groups of microorganisms instead of whole microbial communities could be important to provide stronger evidences supporting our results and conclusions.
Fil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Sistema de Producción Vegetal. Laboratorio Biodiversidad Vegetal y Microbiana Fitopatologia; Argentina
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina
Fil: Gomez, Elena del V.. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Sistema de Producción Vegetal. Laboratorio Biodiversidad Vegetal y Microbiana Fitopatologia; Argentina
description Glyphosate is the most used herbicide worldwide. While contrasting results have been observed related with its impact on soil microbial communities, more studies are necessary to elucidate the potential effects of the herbicide. Differences in tolerance detected by Pollution Induced Community Tolerance (PICT) approach could reflect these effects. The objective of the present study was to assess the tolerance to glyphosate (the active ingredient and a commercial formulation) of contrasting soils with (H) and without (NH) history of exposure. The hypothesis of a higher tolerance in H soils due to a sustained selection pressure on community structure was tested through the PICT approach. Results indicated that tolerance to glyphosate is not consistent with previous history of exposure to the herbicide. Studies of community structure based on Denaturant Gradient Gel Electrophoresis (DGGE) and Quantitative Real Time PCR (Q-PCR) showed that composition and abundance of bacteria, respectively, did not differ significantly between H and NH soils, in accordance with PICT results. Consequently, the lack of significant differences in tolerance in most cases could be partially explained by a similar community structure. This is, to our knowledge, the first report of PICT approach with glyphosate examining tolerance at soil microbial community level. Future culture-independent analysis focused on specific groups of microorganisms instead of whole microbial communities could be important to provide stronger evidences supporting our results and conclusions.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11585
Allegrini, Marco; Zabaloy, Maria Celina; Gomez, Elena del V.; Ecotoxicological assessment of soil microbial community tolerance to glyphosate; Elsevier Science; Science of the Total Environment; 553; 11-2015; 60-68
0048-9697
url http://hdl.handle.net/11336/11585
identifier_str_mv Allegrini, Marco; Zabaloy, Maria Celina; Gomez, Elena del V.; Ecotoxicological assessment of soil microbial community tolerance to glyphosate; Elsevier Science; Science of the Total Environment; 553; 11-2015; 60-68
0048-9697
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0048969715302898
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2015.06.096
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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