Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites

Autores
Richardson, Miles; Ren, Jihui; Rubinstein Guichon, Mara Roxana; Taylor, Jamila A.; Friedman, Richard A.; Shen, Bo; Han, Yiping W.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusobacterium nucleatum is a Gram-negative oral commensal anaerobe which has been increasingly implicated in various gastrointestinal (GI) disorders, including inflammatory bowel disease,appendicitis, GI cancers. The oral cavity harbors a diverse group of Fusobacterium, and it ispostulated that F. nucleatum in the GI tract originate from the mouth. It is not known, however, ifall oral Fusobacterium translocate to the GI sites with equal efficiencies. Therefore, we amplified 16SrRNA genes of F. nucleatum and F. periodonticum, two closely related oral species from matchedsaliva, gastric aspirates, and colon or ileal pouch aspirates of three patients with inflammatorybowel disease (IBD) and three healthy controls, and saliva alone from seven patients with eitheractive IBD or IBD in remission. The 16S rRNA gene amplicons were cloned, and the DNA sequencesdetermined by Sanger sequencing. The results demonstrate that fusobacterial community composition differs more significantly between the oral and GI sites than between different individuals.The oral communities demonstrate the highest level of variation and have the richest pool ofunique sequences, with certain nodes/strains enriched in the GI tract and others diminished duringtranslocation. The gastric and colon/pouch communities exhibit reduced diversity and are moreclosely related, possibly due to selective pressure in the GI tract. This study elucidates selectivetranslocation of oral fusobacteria to the GI tract. Identification of specific transmissible clones willfacilitate risk assessment for developing Fusobacterium-implicated GI disorders.
Fil: Richardson, Miles. Columbia University; Estados Unidos
Fil: Ren, Jihui. Columbia University; Estados Unidos
Fil: Rubinstein Guichon, Mara Roxana. Columbia University; Estados Unidos. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Taylor, Jamila A.. Columbia University; Estados Unidos
Fil: Friedman, Richard A.. Columbia University; Estados Unidos
Fil: Shen, Bo. No especifíca;
Fil: Han, Yiping W.. Columbia University; Estados Unidos
Materia
16S RRNA
COLON ASPIRATE
FUSOBACTERIUM
GASTRIC ASPIRATE
GI TRACT
ILEAL POUCH ASPIRATE
INFLAMMATORY BOWEL DISEASE
SALIVA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133907
Acceder
id CONICETDig_44ff5790c056602d15db34b41598c747
oai_identifier_str oai:ri.conicet.gov.ar:11336/133907
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sitesRichardson, MilesRen, JihuiRubinstein Guichon, Mara RoxanaTaylor, Jamila A.Friedman, Richard A.Shen, BoHan, Yiping W.16S RRNACOLON ASPIRATEFUSOBACTERIUMGASTRIC ASPIRATEGI TRACTILEAL POUCH ASPIRATEINFLAMMATORY BOWEL DISEASESALIVAhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Fusobacterium nucleatum is a Gram-negative oral commensal anaerobe which has been increasingly implicated in various gastrointestinal (GI) disorders, including inflammatory bowel disease,appendicitis, GI cancers. The oral cavity harbors a diverse group of Fusobacterium, and it ispostulated that F. nucleatum in the GI tract originate from the mouth. It is not known, however, ifall oral Fusobacterium translocate to the GI sites with equal efficiencies. Therefore, we amplified 16SrRNA genes of F. nucleatum and F. periodonticum, two closely related oral species from matchedsaliva, gastric aspirates, and colon or ileal pouch aspirates of three patients with inflammatorybowel disease (IBD) and three healthy controls, and saliva alone from seven patients with eitheractive IBD or IBD in remission. The 16S rRNA gene amplicons were cloned, and the DNA sequencesdetermined by Sanger sequencing. The results demonstrate that fusobacterial community composition differs more significantly between the oral and GI sites than between different individuals.The oral communities demonstrate the highest level of variation and have the richest pool ofunique sequences, with certain nodes/strains enriched in the GI tract and others diminished duringtranslocation. The gastric and colon/pouch communities exhibit reduced diversity and are moreclosely related, possibly due to selective pressure in the GI tract. This study elucidates selectivetranslocation of oral fusobacteria to the GI tract. Identification of specific transmissible clones willfacilitate risk assessment for developing Fusobacterium-implicated GI disorders.Fil: Richardson, Miles. Columbia University; Estados UnidosFil: Ren, Jihui. Columbia University; Estados UnidosFil: Rubinstein Guichon, Mara Roxana. Columbia University; Estados Unidos. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Taylor, Jamila A.. Columbia University; Estados UnidosFil: Friedman, Richard A.. Columbia University; Estados UnidosFil: Shen, Bo. No especifíca;Fil: Han, Yiping W.. Columbia University; Estados UnidosWiley2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133907Richardson, Miles; Ren, Jihui; Rubinstein Guichon, Mara Roxana; Taylor, Jamila A.; Friedman, Richard A.; et al.; Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites; Wiley; Gut Microbes; 12; 1; 11-2020; 1-131949-09841949-0984CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/19490976.2020.1814120info:eu-repo/semantics/altIdentifier/doi/10.1080/19490976.2020.1814120info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:26Zoai:ri.conicet.gov.ar:11336/133907instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:27.203CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites
title Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites
spellingShingle Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites
Richardson, Miles
16S RRNA
COLON ASPIRATE
FUSOBACTERIUM
GASTRIC ASPIRATE
GI TRACT
ILEAL POUCH ASPIRATE
INFLAMMATORY BOWEL DISEASE
SALIVA
title_short Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites
title_full Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites
title_fullStr Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites
title_full_unstemmed Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites
title_sort Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites
dc.creator.none.fl_str_mv Richardson, Miles
Ren, Jihui
Rubinstein Guichon, Mara Roxana
Taylor, Jamila A.
Friedman, Richard A.
Shen, Bo
Han, Yiping W.
author Richardson, Miles
author_facet Richardson, Miles
Ren, Jihui
Rubinstein Guichon, Mara Roxana
Taylor, Jamila A.
Friedman, Richard A.
Shen, Bo
Han, Yiping W.
author_role author
author2 Ren, Jihui
Rubinstein Guichon, Mara Roxana
Taylor, Jamila A.
Friedman, Richard A.
Shen, Bo
Han, Yiping W.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv 16S RRNA
COLON ASPIRATE
FUSOBACTERIUM
GASTRIC ASPIRATE
GI TRACT
ILEAL POUCH ASPIRATE
INFLAMMATORY BOWEL DISEASE
SALIVA
topic 16S RRNA
COLON ASPIRATE
FUSOBACTERIUM
GASTRIC ASPIRATE
GI TRACT
ILEAL POUCH ASPIRATE
INFLAMMATORY BOWEL DISEASE
SALIVA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Fusobacterium nucleatum is a Gram-negative oral commensal anaerobe which has been increasingly implicated in various gastrointestinal (GI) disorders, including inflammatory bowel disease,appendicitis, GI cancers. The oral cavity harbors a diverse group of Fusobacterium, and it ispostulated that F. nucleatum in the GI tract originate from the mouth. It is not known, however, ifall oral Fusobacterium translocate to the GI sites with equal efficiencies. Therefore, we amplified 16SrRNA genes of F. nucleatum and F. periodonticum, two closely related oral species from matchedsaliva, gastric aspirates, and colon or ileal pouch aspirates of three patients with inflammatorybowel disease (IBD) and three healthy controls, and saliva alone from seven patients with eitheractive IBD or IBD in remission. The 16S rRNA gene amplicons were cloned, and the DNA sequencesdetermined by Sanger sequencing. The results demonstrate that fusobacterial community composition differs more significantly between the oral and GI sites than between different individuals.The oral communities demonstrate the highest level of variation and have the richest pool ofunique sequences, with certain nodes/strains enriched in the GI tract and others diminished duringtranslocation. The gastric and colon/pouch communities exhibit reduced diversity and are moreclosely related, possibly due to selective pressure in the GI tract. This study elucidates selectivetranslocation of oral fusobacteria to the GI tract. Identification of specific transmissible clones willfacilitate risk assessment for developing Fusobacterium-implicated GI disorders.
Fil: Richardson, Miles. Columbia University; Estados Unidos
Fil: Ren, Jihui. Columbia University; Estados Unidos
Fil: Rubinstein Guichon, Mara Roxana. Columbia University; Estados Unidos. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Taylor, Jamila A.. Columbia University; Estados Unidos
Fil: Friedman, Richard A.. Columbia University; Estados Unidos
Fil: Shen, Bo. No especifíca;
Fil: Han, Yiping W.. Columbia University; Estados Unidos
description Fusobacterium nucleatum is a Gram-negative oral commensal anaerobe which has been increasingly implicated in various gastrointestinal (GI) disorders, including inflammatory bowel disease,appendicitis, GI cancers. The oral cavity harbors a diverse group of Fusobacterium, and it ispostulated that F. nucleatum in the GI tract originate from the mouth. It is not known, however, ifall oral Fusobacterium translocate to the GI sites with equal efficiencies. Therefore, we amplified 16SrRNA genes of F. nucleatum and F. periodonticum, two closely related oral species from matchedsaliva, gastric aspirates, and colon or ileal pouch aspirates of three patients with inflammatorybowel disease (IBD) and three healthy controls, and saliva alone from seven patients with eitheractive IBD or IBD in remission. The 16S rRNA gene amplicons were cloned, and the DNA sequencesdetermined by Sanger sequencing. The results demonstrate that fusobacterial community composition differs more significantly between the oral and GI sites than between different individuals.The oral communities demonstrate the highest level of variation and have the richest pool ofunique sequences, with certain nodes/strains enriched in the GI tract and others diminished duringtranslocation. The gastric and colon/pouch communities exhibit reduced diversity and are moreclosely related, possibly due to selective pressure in the GI tract. This study elucidates selectivetranslocation of oral fusobacteria to the GI tract. Identification of specific transmissible clones willfacilitate risk assessment for developing Fusobacterium-implicated GI disorders.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133907
Richardson, Miles; Ren, Jihui; Rubinstein Guichon, Mara Roxana; Taylor, Jamila A.; Friedman, Richard A.; et al.; Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites; Wiley; Gut Microbes; 12; 1; 11-2020; 1-13
1949-0984
1949-0984
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133907
identifier_str_mv Richardson, Miles; Ren, Jihui; Rubinstein Guichon, Mara Roxana; Taylor, Jamila A.; Friedman, Richard A.; et al.; Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites; Wiley; Gut Microbes; 12; 1; 11-2020; 1-13
1949-0984
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/19490976.2020.1814120
info:eu-repo/semantics/altIdentifier/doi/10.1080/19490976.2020.1814120
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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