Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential

Autores
Paez, Paulina Laura; Becerra, María Cecilia; Albesa, Ines
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study was undertaken to explore the interaction of ciprofloxacin and chloramphenicol with bacterial membranes in a sensitive and in a resistant strains of Staphylococcus aureus by using 1-anilino-8-naphthalene sulfonate (ANS). The binding of this probe to the cell membrane depends on the surface potential, which modulates the binding constant to the membrane. We observed that these antibiotics interacted with the bilayer, thus affecting the electrostatic surface potential. Alterations caused by antibiotics on the surface of the bacteria were accompanied by a reduction in the number of binding sites and an increase in the ANS dissociation constant in the sensitive strain, whereas in the ciprofloxacin-resistant strain no significant changes were detected. The changes seen in the electrostatic surface potential generated in the membrane of S. aureus by the antibiotics provide new aspects concerning their action on the bacterial cell.
Fil: Paez, Paulina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Albesa, Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Materia
Antibiotics
electrostatic surface potential
bacterial membrane
Staphylococcus aureus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9321
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/9321
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane PotentialPaez, Paulina LauraBecerra, María CeciliaAlbesa, InesAntibioticselectrostatic surface potentialbacterial membraneStaphylococcus aureushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The present study was undertaken to explore the interaction of ciprofloxacin and chloramphenicol with bacterial membranes in a sensitive and in a resistant strains of Staphylococcus aureus by using 1-anilino-8-naphthalene sulfonate (ANS). The binding of this probe to the cell membrane depends on the surface potential, which modulates the binding constant to the membrane. We observed that these antibiotics interacted with the bilayer, thus affecting the electrostatic surface potential. Alterations caused by antibiotics on the surface of the bacteria were accompanied by a reduction in the number of binding sites and an increase in the ANS dissociation constant in the sensitive strain, whereas in the ciprofloxacin-resistant strain no significant changes were detected. The changes seen in the electrostatic surface potential generated in the membrane of S. aureus by the antibiotics provide new aspects concerning their action on the bacterial cell.Fil: Paez, Paulina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Albesa, Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaHindawi Publishing Corporation2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9321Paez, Paulina Laura; Becerra, María Cecilia; Albesa, Ines; Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential; Hindawi Publishing Corporation; Biomed Research International; 2013; 5-2013; 1-52314-6141enginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2013/276524info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23762834/info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2013/276524/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:19Zoai:ri.conicet.gov.ar:11336/9321instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:19.364CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential
title Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential
spellingShingle Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential
Paez, Paulina Laura
Antibiotics
electrostatic surface potential
bacterial membrane
Staphylococcus aureus
title_short Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential
title_full Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential
title_fullStr Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential
title_full_unstemmed Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential
title_sort Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential
dc.creator.none.fl_str_mv Paez, Paulina Laura
Becerra, María Cecilia
Albesa, Ines
author Paez, Paulina Laura
author_facet Paez, Paulina Laura
Becerra, María Cecilia
Albesa, Ines
author_role author
author2 Becerra, María Cecilia
Albesa, Ines
author2_role author
author
dc.subject.none.fl_str_mv Antibiotics
electrostatic surface potential
bacterial membrane
Staphylococcus aureus
topic Antibiotics
electrostatic surface potential
bacterial membrane
Staphylococcus aureus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The present study was undertaken to explore the interaction of ciprofloxacin and chloramphenicol with bacterial membranes in a sensitive and in a resistant strains of Staphylococcus aureus by using 1-anilino-8-naphthalene sulfonate (ANS). The binding of this probe to the cell membrane depends on the surface potential, which modulates the binding constant to the membrane. We observed that these antibiotics interacted with the bilayer, thus affecting the electrostatic surface potential. Alterations caused by antibiotics on the surface of the bacteria were accompanied by a reduction in the number of binding sites and an increase in the ANS dissociation constant in the sensitive strain, whereas in the ciprofloxacin-resistant strain no significant changes were detected. The changes seen in the electrostatic surface potential generated in the membrane of S. aureus by the antibiotics provide new aspects concerning their action on the bacterial cell.
Fil: Paez, Paulina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Albesa, Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
description The present study was undertaken to explore the interaction of ciprofloxacin and chloramphenicol with bacterial membranes in a sensitive and in a resistant strains of Staphylococcus aureus by using 1-anilino-8-naphthalene sulfonate (ANS). The binding of this probe to the cell membrane depends on the surface potential, which modulates the binding constant to the membrane. We observed that these antibiotics interacted with the bilayer, thus affecting the electrostatic surface potential. Alterations caused by antibiotics on the surface of the bacteria were accompanied by a reduction in the number of binding sites and an increase in the ANS dissociation constant in the sensitive strain, whereas in the ciprofloxacin-resistant strain no significant changes were detected. The changes seen in the electrostatic surface potential generated in the membrane of S. aureus by the antibiotics provide new aspects concerning their action on the bacterial cell.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9321
Paez, Paulina Laura; Becerra, María Cecilia; Albesa, Ines; Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential; Hindawi Publishing Corporation; Biomed Research International; 2013; 5-2013; 1-5
2314-6141
url http://hdl.handle.net/11336/9321
identifier_str_mv Paez, Paulina Laura; Becerra, María Cecilia; Albesa, Ines; Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer of Staphylococcus aureus: Changes in Membrane Potential; Hindawi Publishing Corporation; Biomed Research International; 2013; 5-2013; 1-5
2314-6141
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/276524
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23762834/
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2013/276524/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269455781986304
score 12.885934

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