Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition

Autores
Ponzo, Osvaldo Juan; Schreier, Laura Ester; Resnik, R.; Negri, Gustavo Alberto; Scacchi, Pablo; Cresta, M. A.; Wikinski, R.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To study if the course of cerulein-induced pancreatitis in rats changes in a state of triglyceride-rich lipoprotein metabolism alteration. Methods: Two groups of rats received control diet during a 90-day period (A) and sucrose-rich diet to induce endogenous hypertriglyceridemia (B). Subgroups A2 and B2 received i.p. 45 μg cerulein/kg body weight (to induce acute pancreatitis). Histological examination of pancreas tissue, serum pancreatic lipase, lipoprotein profile and VLDL chemical composition were assessed. Then, pancreatic lipase hydrolytic activity on VLDL-triglycerides was evaluated in vitro. Results: Cellular vacuolization was observed in all of the cerulean-injected rats, but only in subgroup B2 fat necrosis was present. Serum triglycerides were higher in subgroup B1 than in subgroup A1 (mean ± SEM, mg/ dl 123,77 ± 25.7 vs. 65.8 ± 7, p < 0.01). Triglycerides from rats fed with sucrose-rich diet, decreased after cerulein-induced pancreatitis (80.38 ± 11.3 vs. 123,77 ± 25.7, p < 0.02). Moreover, the endogenous hypertriglyceridemic rats showed an increment of VLDL triglyceride content, which decreased when rats were injected with cerulein. A negative correlation was found between VLDL-triglyceride content and serum pancreatic lipase activity (r = 0.58, p < 0.02). The in vitro assay showed a decrease in VLDL-triglyceride content post incubation with pancreatic lipase enriched serum (mean ± SD: 59.2 ± 27.7%, p < 0.01). Conclusions: The endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis and it could be related to the decrease in VLDL-triglycerides as a consequence of pancreatic lipase hydrolytic activity.
Fil: Ponzo, Osvaldo Juan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Schreier, Laura Ester. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Resnik, R.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Negri, Gustavo Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Scacchi, Pablo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Cresta, M. A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Wikinski, R.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Materia
CERULEIN
HYPERTRIGLYCERIDEMIA
LIPID
PANCREATITIS
VERY-LOW-DENSITY LIPOPROTEIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/112839
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/112839
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL compositionPonzo, Osvaldo JuanSchreier, Laura EsterResnik, R.Negri, Gustavo AlbertoScacchi, PabloCresta, M. A.Wikinski, R.CERULEINHYPERTRIGLYCERIDEMIALIPIDPANCREATITISVERY-LOW-DENSITY LIPOPROTEINhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Aims: To study if the course of cerulein-induced pancreatitis in rats changes in a state of triglyceride-rich lipoprotein metabolism alteration. Methods: Two groups of rats received control diet during a 90-day period (A) and sucrose-rich diet to induce endogenous hypertriglyceridemia (B). Subgroups A2 and B2 received i.p. 45 μg cerulein/kg body weight (to induce acute pancreatitis). Histological examination of pancreas tissue, serum pancreatic lipase, lipoprotein profile and VLDL chemical composition were assessed. Then, pancreatic lipase hydrolytic activity on VLDL-triglycerides was evaluated in vitro. Results: Cellular vacuolization was observed in all of the cerulean-injected rats, but only in subgroup B2 fat necrosis was present. Serum triglycerides were higher in subgroup B1 than in subgroup A1 (mean ± SEM, mg/ dl 123,77 ± 25.7 vs. 65.8 ± 7, p < 0.01). Triglycerides from rats fed with sucrose-rich diet, decreased after cerulein-induced pancreatitis (80.38 ± 11.3 vs. 123,77 ± 25.7, p < 0.02). Moreover, the endogenous hypertriglyceridemic rats showed an increment of VLDL triglyceride content, which decreased when rats were injected with cerulein. A negative correlation was found between VLDL-triglyceride content and serum pancreatic lipase activity (r = 0.58, p < 0.02). The in vitro assay showed a decrease in VLDL-triglyceride content post incubation with pancreatic lipase enriched serum (mean ± SD: 59.2 ± 27.7%, p < 0.01). Conclusions: The endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis and it could be related to the decrease in VLDL-triglycerides as a consequence of pancreatic lipase hydrolytic activity.Fil: Ponzo, Osvaldo Juan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Schreier, Laura Ester. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Resnik, R.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Negri, Gustavo Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Scacchi, Pablo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Cresta, M. A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Wikinski, R.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaKarger2006-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112839Ponzo, Osvaldo Juan; Schreier, Laura Ester; Resnik, R.; Negri, Gustavo Alberto; Scacchi, Pablo; et al.; Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition; Karger; Annals Of Nutrition And Metabolism; 50; 1; 1-2006; 37-440250-6807CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000089563info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/89563info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:31Zoai:ri.conicet.gov.ar:11336/112839instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:32.135CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition
title Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition
spellingShingle Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition
Ponzo, Osvaldo Juan
CERULEIN
HYPERTRIGLYCERIDEMIA
LIPID
PANCREATITIS
VERY-LOW-DENSITY LIPOPROTEIN
title_short Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition
title_full Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition
title_fullStr Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition
title_full_unstemmed Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition
title_sort Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition
dc.creator.none.fl_str_mv Ponzo, Osvaldo Juan
Schreier, Laura Ester
Resnik, R.
Negri, Gustavo Alberto
Scacchi, Pablo
Cresta, M. A.
Wikinski, R.
author Ponzo, Osvaldo Juan
author_facet Ponzo, Osvaldo Juan
Schreier, Laura Ester
Resnik, R.
Negri, Gustavo Alberto
Scacchi, Pablo
Cresta, M. A.
Wikinski, R.
author_role author
author2 Schreier, Laura Ester
Resnik, R.
Negri, Gustavo Alberto
Scacchi, Pablo
Cresta, M. A.
Wikinski, R.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CERULEIN
HYPERTRIGLYCERIDEMIA
LIPID
PANCREATITIS
VERY-LOW-DENSITY LIPOPROTEIN
topic CERULEIN
HYPERTRIGLYCERIDEMIA
LIPID
PANCREATITIS
VERY-LOW-DENSITY LIPOPROTEIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aims: To study if the course of cerulein-induced pancreatitis in rats changes in a state of triglyceride-rich lipoprotein metabolism alteration. Methods: Two groups of rats received control diet during a 90-day period (A) and sucrose-rich diet to induce endogenous hypertriglyceridemia (B). Subgroups A2 and B2 received i.p. 45 μg cerulein/kg body weight (to induce acute pancreatitis). Histological examination of pancreas tissue, serum pancreatic lipase, lipoprotein profile and VLDL chemical composition were assessed. Then, pancreatic lipase hydrolytic activity on VLDL-triglycerides was evaluated in vitro. Results: Cellular vacuolization was observed in all of the cerulean-injected rats, but only in subgroup B2 fat necrosis was present. Serum triglycerides were higher in subgroup B1 than in subgroup A1 (mean ± SEM, mg/ dl 123,77 ± 25.7 vs. 65.8 ± 7, p < 0.01). Triglycerides from rats fed with sucrose-rich diet, decreased after cerulein-induced pancreatitis (80.38 ± 11.3 vs. 123,77 ± 25.7, p < 0.02). Moreover, the endogenous hypertriglyceridemic rats showed an increment of VLDL triglyceride content, which decreased when rats were injected with cerulein. A negative correlation was found between VLDL-triglyceride content and serum pancreatic lipase activity (r = 0.58, p < 0.02). The in vitro assay showed a decrease in VLDL-triglyceride content post incubation with pancreatic lipase enriched serum (mean ± SD: 59.2 ± 27.7%, p < 0.01). Conclusions: The endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis and it could be related to the decrease in VLDL-triglycerides as a consequence of pancreatic lipase hydrolytic activity.
Fil: Ponzo, Osvaldo Juan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Schreier, Laura Ester. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Resnik, R.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Negri, Gustavo Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Scacchi, Pablo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Cresta, M. A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Wikinski, R.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
description Aims: To study if the course of cerulein-induced pancreatitis in rats changes in a state of triglyceride-rich lipoprotein metabolism alteration. Methods: Two groups of rats received control diet during a 90-day period (A) and sucrose-rich diet to induce endogenous hypertriglyceridemia (B). Subgroups A2 and B2 received i.p. 45 μg cerulein/kg body weight (to induce acute pancreatitis). Histological examination of pancreas tissue, serum pancreatic lipase, lipoprotein profile and VLDL chemical composition were assessed. Then, pancreatic lipase hydrolytic activity on VLDL-triglycerides was evaluated in vitro. Results: Cellular vacuolization was observed in all of the cerulean-injected rats, but only in subgroup B2 fat necrosis was present. Serum triglycerides were higher in subgroup B1 than in subgroup A1 (mean ± SEM, mg/ dl 123,77 ± 25.7 vs. 65.8 ± 7, p < 0.01). Triglycerides from rats fed with sucrose-rich diet, decreased after cerulein-induced pancreatitis (80.38 ± 11.3 vs. 123,77 ± 25.7, p < 0.02). Moreover, the endogenous hypertriglyceridemic rats showed an increment of VLDL triglyceride content, which decreased when rats were injected with cerulein. A negative correlation was found between VLDL-triglyceride content and serum pancreatic lipase activity (r = 0.58, p < 0.02). The in vitro assay showed a decrease in VLDL-triglyceride content post incubation with pancreatic lipase enriched serum (mean ± SD: 59.2 ± 27.7%, p < 0.01). Conclusions: The endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis and it could be related to the decrease in VLDL-triglycerides as a consequence of pancreatic lipase hydrolytic activity.
publishDate 2006
dc.date.none.fl_str_mv 2006-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112839
Ponzo, Osvaldo Juan; Schreier, Laura Ester; Resnik, R.; Negri, Gustavo Alberto; Scacchi, Pablo; et al.; Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition; Karger; Annals Of Nutrition And Metabolism; 50; 1; 1-2006; 37-44
0250-6807
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112839
identifier_str_mv Ponzo, Osvaldo Juan; Schreier, Laura Ester; Resnik, R.; Negri, Gustavo Alberto; Scacchi, Pablo; et al.; Endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis in rat: Relation with changes in the VLDL composition; Karger; Annals Of Nutrition And Metabolism; 50; 1; 1-2006; 37-44
0250-6807
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000089563
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/89563
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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