Paroxymal nocturnal haemoglobinuria: experience over 10 years period

Autores
Canalejo, K.; Riera Cervantes, Norma Edith; Felippo, Marta Elena; Sarandría, C.; Aixalá, M.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Paroxysmal nocturnal hemoglobinuria (PNH) is a hemolytic, clonal and acquired disorder of the hematopoietic stem cell with a deficiency of all glycophosphatidyl-inositol (GPI) linked proteins. The aim of this retrospective study was to analyse haematological and biochemical data from 152 patients referred to our laboratory for diagnosis of PNH by flow cytometry (FC). Methods: Patients and healthy donor (152 and 99 respectively) were studied. Ham, sucrose, lactate dehydrogenase (LDH), Iron, haptoglobin (Hp), blood cell morphology and Kaplow cytochemical stain for leukocyte alkaline phosphatase (LAP) were carried out. GPI-proteins anti-CD55 and CD59 in erythrocytes and the former, plus anti CD16b and CD66b on neutrophils were evaluated by FC. Results: Anemia and/or leukopenia and/or thrombocytopenia, increased reticulocyte count and LDH were observed in patients with PNH clone. Some of them had dacriocytes, schistocytes. LAP was low. On average, we detected 50% CD59 (−) erythrocytes and 29, 83, 78% CD55/59 (−), CD16b (−), CD66b (−) neutrophils, respectively. Conclusion: Paroxysmal nocturnal hemoglobinuria clone was detected in 20/152 patients. Negative population's percentages were high in patients with classic PNH, Hematimetry, LAP and adequate use of CF contribute to PNH clone detection in the laboratory.
Fil: Canalejo, K.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Riera Cervantes, Norma Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Felippo, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Sarandría, C.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Aixalá, M.. Academia Nacional de Medicina de Buenos Aires; Argentina
Materia
Neutropenia
Anti-Pmn Antibodies
Flow Cytometry
Gift- Cd16blow
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20803
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/20803
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Paroxymal nocturnal haemoglobinuria: experience over 10 years periodCanalejo, K.Riera Cervantes, Norma EdithFelippo, Marta ElenaSarandría, C.Aixalá, M.NeutropeniaAnti-Pmn AntibodiesFlow CytometryGift- Cd16blowhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Introduction: Paroxysmal nocturnal hemoglobinuria (PNH) is a hemolytic, clonal and acquired disorder of the hematopoietic stem cell with a deficiency of all glycophosphatidyl-inositol (GPI) linked proteins. The aim of this retrospective study was to analyse haematological and biochemical data from 152 patients referred to our laboratory for diagnosis of PNH by flow cytometry (FC). Methods: Patients and healthy donor (152 and 99 respectively) were studied. Ham, sucrose, lactate dehydrogenase (LDH), Iron, haptoglobin (Hp), blood cell morphology and Kaplow cytochemical stain for leukocyte alkaline phosphatase (LAP) were carried out. GPI-proteins anti-CD55 and CD59 in erythrocytes and the former, plus anti CD16b and CD66b on neutrophils were evaluated by FC. Results: Anemia and/or leukopenia and/or thrombocytopenia, increased reticulocyte count and LDH were observed in patients with PNH clone. Some of them had dacriocytes, schistocytes. LAP was low. On average, we detected 50% CD59 (−) erythrocytes and 29, 83, 78% CD55/59 (−), CD16b (−), CD66b (−) neutrophils, respectively. Conclusion: Paroxysmal nocturnal hemoglobinuria clone was detected in 20/152 patients. Negative population's percentages were high in patients with classic PNH, Hematimetry, LAP and adequate use of CF contribute to PNH clone detection in the laboratory.Fil: Canalejo, K.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Riera Cervantes, Norma Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Felippo, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Sarandría, C.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Aixalá, M.. Academia Nacional de Medicina de Buenos Aires; ArgentinaWiley2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20803Canalejo, K.; Riera Cervantes, Norma Edith; Felippo, Marta Elena; Sarandría, C.; Aixalá, M.; Paroxymal nocturnal haemoglobinuria: experience over 10 years period; Wiley; International Journal of Laboratory Hematology; 36; 2; 4-2014; 213-2211751-55211751-553XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/ijlh.12156info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ijlh.12156/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:33Zoai:ri.conicet.gov.ar:11336/20803instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:33.889CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Paroxymal nocturnal haemoglobinuria: experience over 10 years period
title Paroxymal nocturnal haemoglobinuria: experience over 10 years period
spellingShingle Paroxymal nocturnal haemoglobinuria: experience over 10 years period
Canalejo, K.
Neutropenia
Anti-Pmn Antibodies
Flow Cytometry
Gift- Cd16blow
title_short Paroxymal nocturnal haemoglobinuria: experience over 10 years period
title_full Paroxymal nocturnal haemoglobinuria: experience over 10 years period
title_fullStr Paroxymal nocturnal haemoglobinuria: experience over 10 years period
title_full_unstemmed Paroxymal nocturnal haemoglobinuria: experience over 10 years period
title_sort Paroxymal nocturnal haemoglobinuria: experience over 10 years period
dc.creator.none.fl_str_mv Canalejo, K.
Riera Cervantes, Norma Edith
Felippo, Marta Elena
Sarandría, C.
Aixalá, M.
author Canalejo, K.
author_facet Canalejo, K.
Riera Cervantes, Norma Edith
Felippo, Marta Elena
Sarandría, C.
Aixalá, M.
author_role author
author2 Riera Cervantes, Norma Edith
Felippo, Marta Elena
Sarandría, C.
Aixalá, M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Neutropenia
Anti-Pmn Antibodies
Flow Cytometry
Gift- Cd16blow
topic Neutropenia
Anti-Pmn Antibodies
Flow Cytometry
Gift- Cd16blow
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction: Paroxysmal nocturnal hemoglobinuria (PNH) is a hemolytic, clonal and acquired disorder of the hematopoietic stem cell with a deficiency of all glycophosphatidyl-inositol (GPI) linked proteins. The aim of this retrospective study was to analyse haematological and biochemical data from 152 patients referred to our laboratory for diagnosis of PNH by flow cytometry (FC). Methods: Patients and healthy donor (152 and 99 respectively) were studied. Ham, sucrose, lactate dehydrogenase (LDH), Iron, haptoglobin (Hp), blood cell morphology and Kaplow cytochemical stain for leukocyte alkaline phosphatase (LAP) were carried out. GPI-proteins anti-CD55 and CD59 in erythrocytes and the former, plus anti CD16b and CD66b on neutrophils were evaluated by FC. Results: Anemia and/or leukopenia and/or thrombocytopenia, increased reticulocyte count and LDH were observed in patients with PNH clone. Some of them had dacriocytes, schistocytes. LAP was low. On average, we detected 50% CD59 (−) erythrocytes and 29, 83, 78% CD55/59 (−), CD16b (−), CD66b (−) neutrophils, respectively. Conclusion: Paroxysmal nocturnal hemoglobinuria clone was detected in 20/152 patients. Negative population's percentages were high in patients with classic PNH, Hematimetry, LAP and adequate use of CF contribute to PNH clone detection in the laboratory.
Fil: Canalejo, K.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Riera Cervantes, Norma Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Felippo, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Sarandría, C.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Aixalá, M.. Academia Nacional de Medicina de Buenos Aires; Argentina
description Introduction: Paroxysmal nocturnal hemoglobinuria (PNH) is a hemolytic, clonal and acquired disorder of the hematopoietic stem cell with a deficiency of all glycophosphatidyl-inositol (GPI) linked proteins. The aim of this retrospective study was to analyse haematological and biochemical data from 152 patients referred to our laboratory for diagnosis of PNH by flow cytometry (FC). Methods: Patients and healthy donor (152 and 99 respectively) were studied. Ham, sucrose, lactate dehydrogenase (LDH), Iron, haptoglobin (Hp), blood cell morphology and Kaplow cytochemical stain for leukocyte alkaline phosphatase (LAP) were carried out. GPI-proteins anti-CD55 and CD59 in erythrocytes and the former, plus anti CD16b and CD66b on neutrophils were evaluated by FC. Results: Anemia and/or leukopenia and/or thrombocytopenia, increased reticulocyte count and LDH were observed in patients with PNH clone. Some of them had dacriocytes, schistocytes. LAP was low. On average, we detected 50% CD59 (−) erythrocytes and 29, 83, 78% CD55/59 (−), CD16b (−), CD66b (−) neutrophils, respectively. Conclusion: Paroxysmal nocturnal hemoglobinuria clone was detected in 20/152 patients. Negative population's percentages were high in patients with classic PNH, Hematimetry, LAP and adequate use of CF contribute to PNH clone detection in the laboratory.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20803
Canalejo, K.; Riera Cervantes, Norma Edith; Felippo, Marta Elena; Sarandría, C.; Aixalá, M.; Paroxymal nocturnal haemoglobinuria: experience over 10 years period; Wiley; International Journal of Laboratory Hematology; 36; 2; 4-2014; 213-221
1751-5521
1751-553X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20803
identifier_str_mv Canalejo, K.; Riera Cervantes, Norma Edith; Felippo, Marta Elena; Sarandría, C.; Aixalá, M.; Paroxymal nocturnal haemoglobinuria: experience over 10 years period; Wiley; International Journal of Laboratory Hematology; 36; 2; 4-2014; 213-221
1751-5521
1751-553X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/ijlh.12156
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ijlh.12156/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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