Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia

Autores
Riera Cervantes, Norma Edith; Rosso Saltó, M.; Galán, V.; Canalejo, K.; Khoury, M.; Aixalá, M.; Kantor, G. L.; Vermeulen, Elba Monica; Bengló, R.; de Elizalde, Maria Marta
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Immune humoral neutropenia (Np) could be the consequence of anti-polymorphonuclear neutrophil (PMN) antibodies, circulating immune complexes (CIC) and/or antibodies against myeloid precursors. Granulocyte immunofluorescence test (GIFT) and a leukoagglutination technique (LAGT) assays are recommended for its diagnosis. Methods: Fifty adult patients with secondary Np were screened for anti-PMN. GIFT by flow cytometry from viable PMN and LAGT were employed. In addition, CIC levels, low expression of CD16b (CD16 blow), PMN phenotype and sera tumor necrosis factor- alpha (TNF-α) were also evaluated. Results: Direct IgG-PMN binding (dir-GIFT) was positive in 16% of the patients. Antibodies against autologous PMN were detected in 32% of the samples by indirect (ind)-GIFT and demonstrated in 70% of the sera by both ind-GIFT and/ or LAGT. Predominance of human neutrophil alloantigen (HNA)-1b and HNA-2 expression was confirmed. CD16b low was detected in 16% of the patient's PMN and TNF-α in 68% of sera patients. Conclusion:Our results suggest that diagnosis of immune Np in the laboratory may be improved by focusing on patient's PMN together with the assessment of cellular markers. © 2009 Blackwell Publishing Ltd.
Fil: Riera Cervantes, Norma Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Rosso Saltó, M.. Asociación Española Primera de Socorros Mutuos Montevideo; Uruguay
Fil: Galán, V.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Canalejo, K.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Khoury, M.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Aixalá, M.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Kantor, G. L.. Hospital Durand; Argentina
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Bengló, R.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: de Elizalde, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Materia
Anti-Pmn Antibodies
Cd16low
Flow Cytometry
Gift
Neutropenia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53662
Acceder
id CONICETDig_e4f3ddea9e635d557ce08f9ec3e63038
oai_identifier_str oai:ri.conicet.gov.ar:11336/53662
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropeniaRiera Cervantes, Norma EdithRosso Saltó, M.Galán, V.Canalejo, K.Khoury, M.Aixalá, M.Kantor, G. L.Vermeulen, Elba MonicaBengló, R.de Elizalde, Maria MartaAnti-Pmn AntibodiesCd16lowFlow CytometryGiftNeutropeniahttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Introduction: Immune humoral neutropenia (Np) could be the consequence of anti-polymorphonuclear neutrophil (PMN) antibodies, circulating immune complexes (CIC) and/or antibodies against myeloid precursors. Granulocyte immunofluorescence test (GIFT) and a leukoagglutination technique (LAGT) assays are recommended for its diagnosis. Methods: Fifty adult patients with secondary Np were screened for anti-PMN. GIFT by flow cytometry from viable PMN and LAGT were employed. In addition, CIC levels, low expression of CD16b (CD16 blow), PMN phenotype and sera tumor necrosis factor- alpha (TNF-α) were also evaluated. Results: Direct IgG-PMN binding (dir-GIFT) was positive in 16% of the patients. Antibodies against autologous PMN were detected in 32% of the samples by indirect (ind)-GIFT and demonstrated in 70% of the sera by both ind-GIFT and/ or LAGT. Predominance of human neutrophil alloantigen (HNA)-1b and HNA-2 expression was confirmed. CD16b low was detected in 16% of the patient's PMN and TNF-α in 68% of sera patients. Conclusion:Our results suggest that diagnosis of immune Np in the laboratory may be improved by focusing on patient's PMN together with the assessment of cellular markers. © 2009 Blackwell Publishing Ltd.Fil: Riera Cervantes, Norma Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Rosso Saltó, M.. Asociación Española Primera de Socorros Mutuos Montevideo; UruguayFil: Galán, V.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Canalejo, K.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Khoury, M.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Aixalá, M.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Kantor, G. L.. Hospital Durand; ArgentinaFil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Bengló, R.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: de Elizalde, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaWiley Blackwell Publishing, Inc2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53662Riera Cervantes, Norma Edith; Rosso Saltó, M.; Galán, V.; Canalejo, K.; Khoury, M.; et al.; Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia; Wiley Blackwell Publishing, Inc; International Journal of Laboratory Hematology; 32; 1; 2-2010; 96-1051751-5521CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-553X.2009.01173.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1751-553X.2009.01173.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:06Zoai:ri.conicet.gov.ar:11336/53662instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:06.268CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia
title Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia
spellingShingle Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia
Riera Cervantes, Norma Edith
Anti-Pmn Antibodies
Cd16low
Flow Cytometry
Gift
Neutropenia
title_short Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia
title_full Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia
title_fullStr Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia
title_full_unstemmed Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia
title_sort Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia
dc.creator.none.fl_str_mv Riera Cervantes, Norma Edith
Rosso Saltó, M.
Galán, V.
Canalejo, K.
Khoury, M.
Aixalá, M.
Kantor, G. L.
Vermeulen, Elba Monica
Bengló, R.
de Elizalde, Maria Marta
author Riera Cervantes, Norma Edith
author_facet Riera Cervantes, Norma Edith
Rosso Saltó, M.
Galán, V.
Canalejo, K.
Khoury, M.
Aixalá, M.
Kantor, G. L.
Vermeulen, Elba Monica
Bengló, R.
de Elizalde, Maria Marta
author_role author
author2 Rosso Saltó, M.
Galán, V.
Canalejo, K.
Khoury, M.
Aixalá, M.
Kantor, G. L.
Vermeulen, Elba Monica
Bengló, R.
de Elizalde, Maria Marta
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anti-Pmn Antibodies
Cd16low
Flow Cytometry
Gift
Neutropenia
topic Anti-Pmn Antibodies
Cd16low
Flow Cytometry
Gift
Neutropenia
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction: Immune humoral neutropenia (Np) could be the consequence of anti-polymorphonuclear neutrophil (PMN) antibodies, circulating immune complexes (CIC) and/or antibodies against myeloid precursors. Granulocyte immunofluorescence test (GIFT) and a leukoagglutination technique (LAGT) assays are recommended for its diagnosis. Methods: Fifty adult patients with secondary Np were screened for anti-PMN. GIFT by flow cytometry from viable PMN and LAGT were employed. In addition, CIC levels, low expression of CD16b (CD16 blow), PMN phenotype and sera tumor necrosis factor- alpha (TNF-α) were also evaluated. Results: Direct IgG-PMN binding (dir-GIFT) was positive in 16% of the patients. Antibodies against autologous PMN were detected in 32% of the samples by indirect (ind)-GIFT and demonstrated in 70% of the sera by both ind-GIFT and/ or LAGT. Predominance of human neutrophil alloantigen (HNA)-1b and HNA-2 expression was confirmed. CD16b low was detected in 16% of the patient's PMN and TNF-α in 68% of sera patients. Conclusion:Our results suggest that diagnosis of immune Np in the laboratory may be improved by focusing on patient's PMN together with the assessment of cellular markers. © 2009 Blackwell Publishing Ltd.
Fil: Riera Cervantes, Norma Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Rosso Saltó, M.. Asociación Española Primera de Socorros Mutuos Montevideo; Uruguay
Fil: Galán, V.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Canalejo, K.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Khoury, M.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Aixalá, M.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Kantor, G. L.. Hospital Durand; Argentina
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Bengló, R.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: de Elizalde, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
description Introduction: Immune humoral neutropenia (Np) could be the consequence of anti-polymorphonuclear neutrophil (PMN) antibodies, circulating immune complexes (CIC) and/or antibodies against myeloid precursors. Granulocyte immunofluorescence test (GIFT) and a leukoagglutination technique (LAGT) assays are recommended for its diagnosis. Methods: Fifty adult patients with secondary Np were screened for anti-PMN. GIFT by flow cytometry from viable PMN and LAGT were employed. In addition, CIC levels, low expression of CD16b (CD16 blow), PMN phenotype and sera tumor necrosis factor- alpha (TNF-α) were also evaluated. Results: Direct IgG-PMN binding (dir-GIFT) was positive in 16% of the patients. Antibodies against autologous PMN were detected in 32% of the samples by indirect (ind)-GIFT and demonstrated in 70% of the sera by both ind-GIFT and/ or LAGT. Predominance of human neutrophil alloantigen (HNA)-1b and HNA-2 expression was confirmed. CD16b low was detected in 16% of the patient's PMN and TNF-α in 68% of sera patients. Conclusion:Our results suggest that diagnosis of immune Np in the laboratory may be improved by focusing on patient's PMN together with the assessment of cellular markers. © 2009 Blackwell Publishing Ltd.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53662
Riera Cervantes, Norma Edith; Rosso Saltó, M.; Galán, V.; Canalejo, K.; Khoury, M.; et al.; Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia; Wiley Blackwell Publishing, Inc; International Journal of Laboratory Hematology; 32; 1; 2-2010; 96-105
1751-5521
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53662
identifier_str_mv Riera Cervantes, Norma Edith; Rosso Saltó, M.; Galán, V.; Canalejo, K.; Khoury, M.; et al.; Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia; Wiley Blackwell Publishing, Inc; International Journal of Laboratory Hematology; 32; 1; 2-2010; 96-105
1751-5521
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-553X.2009.01173.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1751-553X.2009.01173.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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