Tissue factor as a novel marker for detection of circulating cancer cells
- Autores
- Otero, L. L.; Alonso, Daniel Fernando; Castro, M.; Cinat, G.; Gabri, M. R.; Gomez, Daniel Eduardo
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Tissue factor (TF) is a molecular marker that is up-regulated in cancer cells and aids tumoral dissemination. Our purpose was to develop a nested RT-PCR strategy against TF for detecting blood-borne tumour cells. Our method detected TF expression in a minimum of 1.5 pg total RNA from MCF7 cells. A preliminary study in blood samples from 16 advanced breast carcinoma patients showed that 80% of patients with high TF load progressed and died, while only 18% with low TF load showed the same behaviour. Kaplan-Meier analysis confirmed worse overall survival in patients with high TF load.
Fil: Otero, L. L.. Universidad Nacional de Quilmes; Argentina
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castro, M.. Universidad de Buenos Aires; Argentina
Fil: Cinat, G.. Universidad de Buenos Aires; Argentina
Fil: Gabri, M. R.. Universidad Nacional de Quilmes; Argentina
Fil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
BIOMARKER
BREAST CANCER
NESTED PCR
RT-PCR
TISSUE FACTOR - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/191950
Ver los metadatos del registro completo
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Tissue factor as a novel marker for detection of circulating cancer cellsOtero, L. L.Alonso, Daniel FernandoCastro, M.Cinat, G.Gabri, M. R.Gomez, Daniel EduardoBIOMARKERBREAST CANCERNESTED PCRRT-PCRTISSUE FACTORhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Tissue factor (TF) is a molecular marker that is up-regulated in cancer cells and aids tumoral dissemination. Our purpose was to develop a nested RT-PCR strategy against TF for detecting blood-borne tumour cells. Our method detected TF expression in a minimum of 1.5 pg total RNA from MCF7 cells. A preliminary study in blood samples from 16 advanced breast carcinoma patients showed that 80% of patients with high TF load progressed and died, while only 18% with low TF load showed the same behaviour. Kaplan-Meier analysis confirmed worse overall survival in patients with high TF load.Fil: Otero, L. L.. Universidad Nacional de Quilmes; ArgentinaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Castro, M.. Universidad de Buenos Aires; ArgentinaFil: Cinat, G.. Universidad de Buenos Aires; ArgentinaFil: Gabri, M. R.. Universidad Nacional de Quilmes; ArgentinaFil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTaylor & Francis Ltd2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/191950Otero, L. L.; Alonso, Daniel Fernando; Castro, M.; Cinat, G.; Gabri, M. R.; et al.; Tissue factor as a novel marker for detection of circulating cancer cells; Taylor & Francis Ltd; Biomarkers; 16; 1; 2-2011; 58-641354-750XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.3109/1354750X.2010.533282?journalCode=ibmk20info:eu-repo/semantics/altIdentifier/doi/10.3109/1354750X.2010.533282info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:51Zoai:ri.conicet.gov.ar:11336/191950instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:51.936CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Tissue factor as a novel marker for detection of circulating cancer cells |
title |
Tissue factor as a novel marker for detection of circulating cancer cells |
spellingShingle |
Tissue factor as a novel marker for detection of circulating cancer cells Otero, L. L. BIOMARKER BREAST CANCER NESTED PCR RT-PCR TISSUE FACTOR |
title_short |
Tissue factor as a novel marker for detection of circulating cancer cells |
title_full |
Tissue factor as a novel marker for detection of circulating cancer cells |
title_fullStr |
Tissue factor as a novel marker for detection of circulating cancer cells |
title_full_unstemmed |
Tissue factor as a novel marker for detection of circulating cancer cells |
title_sort |
Tissue factor as a novel marker for detection of circulating cancer cells |
dc.creator.none.fl_str_mv |
Otero, L. L. Alonso, Daniel Fernando Castro, M. Cinat, G. Gabri, M. R. Gomez, Daniel Eduardo |
author |
Otero, L. L. |
author_facet |
Otero, L. L. Alonso, Daniel Fernando Castro, M. Cinat, G. Gabri, M. R. Gomez, Daniel Eduardo |
author_role |
author |
author2 |
Alonso, Daniel Fernando Castro, M. Cinat, G. Gabri, M. R. Gomez, Daniel Eduardo |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
BIOMARKER BREAST CANCER NESTED PCR RT-PCR TISSUE FACTOR |
topic |
BIOMARKER BREAST CANCER NESTED PCR RT-PCR TISSUE FACTOR |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Tissue factor (TF) is a molecular marker that is up-regulated in cancer cells and aids tumoral dissemination. Our purpose was to develop a nested RT-PCR strategy against TF for detecting blood-borne tumour cells. Our method detected TF expression in a minimum of 1.5 pg total RNA from MCF7 cells. A preliminary study in blood samples from 16 advanced breast carcinoma patients showed that 80% of patients with high TF load progressed and died, while only 18% with low TF load showed the same behaviour. Kaplan-Meier analysis confirmed worse overall survival in patients with high TF load. Fil: Otero, L. L.. Universidad Nacional de Quilmes; Argentina Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Castro, M.. Universidad de Buenos Aires; Argentina Fil: Cinat, G.. Universidad de Buenos Aires; Argentina Fil: Gabri, M. R.. Universidad Nacional de Quilmes; Argentina Fil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Tissue factor (TF) is a molecular marker that is up-regulated in cancer cells and aids tumoral dissemination. Our purpose was to develop a nested RT-PCR strategy against TF for detecting blood-borne tumour cells. Our method detected TF expression in a minimum of 1.5 pg total RNA from MCF7 cells. A preliminary study in blood samples from 16 advanced breast carcinoma patients showed that 80% of patients with high TF load progressed and died, while only 18% with low TF load showed the same behaviour. Kaplan-Meier analysis confirmed worse overall survival in patients with high TF load. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/191950 Otero, L. L.; Alonso, Daniel Fernando; Castro, M.; Cinat, G.; Gabri, M. R.; et al.; Tissue factor as a novel marker for detection of circulating cancer cells; Taylor & Francis Ltd; Biomarkers; 16; 1; 2-2011; 58-64 1354-750X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/191950 |
identifier_str_mv |
Otero, L. L.; Alonso, Daniel Fernando; Castro, M.; Cinat, G.; Gabri, M. R.; et al.; Tissue factor as a novel marker for detection of circulating cancer cells; Taylor & Francis Ltd; Biomarkers; 16; 1; 2-2011; 58-64 1354-750X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.3109/1354750X.2010.533282?journalCode=ibmk20 info:eu-repo/semantics/altIdentifier/doi/10.3109/1354750X.2010.533282 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis Ltd |
publisher.none.fl_str_mv |
Taylor & Francis Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613260856262656 |
score |
13.070432 |