Tissue factor as a novel marker for detection of circulating cancer cells

Autores
Otero, L. L.; Alonso, Daniel Fernando; Castro, M.; Cinat, G.; Gabri, M. R.; Gomez, Daniel Eduardo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tissue factor (TF) is a molecular marker that is up-regulated in cancer cells and aids tumoral dissemination. Our purpose was to develop a nested RT-PCR strategy against TF for detecting blood-borne tumour cells. Our method detected TF expression in a minimum of 1.5 pg total RNA from MCF7 cells. A preliminary study in blood samples from 16 advanced breast carcinoma patients showed that 80% of patients with high TF load progressed and died, while only 18% with low TF load showed the same behaviour. Kaplan-Meier analysis confirmed worse overall survival in patients with high TF load.
Fil: Otero, L. L.. Universidad Nacional de Quilmes; Argentina
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castro, M.. Universidad de Buenos Aires; Argentina
Fil: Cinat, G.. Universidad de Buenos Aires; Argentina
Fil: Gabri, M. R.. Universidad Nacional de Quilmes; Argentina
Fil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
BIOMARKER
BREAST CANCER
NESTED PCR
RT-PCR
TISSUE FACTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/191950
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Tissue factor as a novel marker for detection of circulating cancer cellsOtero, L. L.Alonso, Daniel FernandoCastro, M.Cinat, G.Gabri, M. R.Gomez, Daniel EduardoBIOMARKERBREAST CANCERNESTED PCRRT-PCRTISSUE FACTORhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Tissue factor (TF) is a molecular marker that is up-regulated in cancer cells and aids tumoral dissemination. Our purpose was to develop a nested RT-PCR strategy against TF for detecting blood-borne tumour cells. Our method detected TF expression in a minimum of 1.5 pg total RNA from MCF7 cells. A preliminary study in blood samples from 16 advanced breast carcinoma patients showed that 80% of patients with high TF load progressed and died, while only 18% with low TF load showed the same behaviour. Kaplan-Meier analysis confirmed worse overall survival in patients with high TF load.Fil: Otero, L. L.. Universidad Nacional de Quilmes; ArgentinaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Castro, M.. Universidad de Buenos Aires; ArgentinaFil: Cinat, G.. Universidad de Buenos Aires; ArgentinaFil: Gabri, M. R.. Universidad Nacional de Quilmes; ArgentinaFil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTaylor & Francis Ltd2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/191950Otero, L. L.; Alonso, Daniel Fernando; Castro, M.; Cinat, G.; Gabri, M. R.; et al.; Tissue factor as a novel marker for detection of circulating cancer cells; Taylor & Francis Ltd; Biomarkers; 16; 1; 2-2011; 58-641354-750XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.3109/1354750X.2010.533282?journalCode=ibmk20info:eu-repo/semantics/altIdentifier/doi/10.3109/1354750X.2010.533282info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:29:22Zoai:ri.conicet.gov.ar:11336/191950instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:29:22.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tissue factor as a novel marker for detection of circulating cancer cells
title Tissue factor as a novel marker for detection of circulating cancer cells
spellingShingle Tissue factor as a novel marker for detection of circulating cancer cells
Otero, L. L.
BIOMARKER
BREAST CANCER
NESTED PCR
RT-PCR
TISSUE FACTOR
title_short Tissue factor as a novel marker for detection of circulating cancer cells
title_full Tissue factor as a novel marker for detection of circulating cancer cells
title_fullStr Tissue factor as a novel marker for detection of circulating cancer cells
title_full_unstemmed Tissue factor as a novel marker for detection of circulating cancer cells
title_sort Tissue factor as a novel marker for detection of circulating cancer cells
dc.creator.none.fl_str_mv Otero, L. L.
Alonso, Daniel Fernando
Castro, M.
Cinat, G.
Gabri, M. R.
Gomez, Daniel Eduardo
author Otero, L. L.
author_facet Otero, L. L.
Alonso, Daniel Fernando
Castro, M.
Cinat, G.
Gabri, M. R.
Gomez, Daniel Eduardo
author_role author
author2 Alonso, Daniel Fernando
Castro, M.
Cinat, G.
Gabri, M. R.
Gomez, Daniel Eduardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOMARKER
BREAST CANCER
NESTED PCR
RT-PCR
TISSUE FACTOR
topic BIOMARKER
BREAST CANCER
NESTED PCR
RT-PCR
TISSUE FACTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Tissue factor (TF) is a molecular marker that is up-regulated in cancer cells and aids tumoral dissemination. Our purpose was to develop a nested RT-PCR strategy against TF for detecting blood-borne tumour cells. Our method detected TF expression in a minimum of 1.5 pg total RNA from MCF7 cells. A preliminary study in blood samples from 16 advanced breast carcinoma patients showed that 80% of patients with high TF load progressed and died, while only 18% with low TF load showed the same behaviour. Kaplan-Meier analysis confirmed worse overall survival in patients with high TF load.
Fil: Otero, L. L.. Universidad Nacional de Quilmes; Argentina
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castro, M.. Universidad de Buenos Aires; Argentina
Fil: Cinat, G.. Universidad de Buenos Aires; Argentina
Fil: Gabri, M. R.. Universidad Nacional de Quilmes; Argentina
Fil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Tissue factor (TF) is a molecular marker that is up-regulated in cancer cells and aids tumoral dissemination. Our purpose was to develop a nested RT-PCR strategy against TF for detecting blood-borne tumour cells. Our method detected TF expression in a minimum of 1.5 pg total RNA from MCF7 cells. A preliminary study in blood samples from 16 advanced breast carcinoma patients showed that 80% of patients with high TF load progressed and died, while only 18% with low TF load showed the same behaviour. Kaplan-Meier analysis confirmed worse overall survival in patients with high TF load.
publishDate 2011
dc.date.none.fl_str_mv 2011-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/191950
Otero, L. L.; Alonso, Daniel Fernando; Castro, M.; Cinat, G.; Gabri, M. R.; et al.; Tissue factor as a novel marker for detection of circulating cancer cells; Taylor & Francis Ltd; Biomarkers; 16; 1; 2-2011; 58-64
1354-750X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/191950
identifier_str_mv Otero, L. L.; Alonso, Daniel Fernando; Castro, M.; Cinat, G.; Gabri, M. R.; et al.; Tissue factor as a novel marker for detection of circulating cancer cells; Taylor & Francis Ltd; Biomarkers; 16; 1; 2-2011; 58-64
1354-750X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.3109/1354750X.2010.533282?journalCode=ibmk20
info:eu-repo/semantics/altIdentifier/doi/10.3109/1354750X.2010.533282
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082763803852800
score 13.22299

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