Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR

Autores
Pisano, María Belén; Sánchez Seco, María Paz; Ré, Viviana Elizabeth; Farías, Adrián A.; Contigiani, Marta Silvia; Tenorio, Antonio
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance.
Fil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sánchez Seco, María Paz. Instituto de Salud Carlos III; España
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Farías, Adrián A.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Contigiani, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Tenorio, Antonio. Instituto de Salud Carlos III; España
Materia
ALPHAVIRUS
ARBOVIRUS
DIAGNOSTIC
RT-NESTED PCR
SURVEILLANCE
VEEV
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199136
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/199136
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCRPisano, María BelénSánchez Seco, María PazRé, Viviana ElizabethFarías, Adrián A.Contigiani, Marta SilviaTenorio, AntonioALPHAVIRUSARBOVIRUSDIAGNOSTICRT-NESTED PCRSURVEILLANCEVEEVhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance.Fil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Sánchez Seco, María Paz. Instituto de Salud Carlos III; EspañaFil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Farías, Adrián A.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Contigiani, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Tenorio, Antonio. Instituto de Salud Carlos III; EspañaElsevier Science2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199136Pisano, María Belén; Sánchez Seco, María Paz; Ré, Viviana Elizabeth; Farías, Adrián A.; Contigiani, Marta Silvia; et al.; Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR; Elsevier Science; Journal of Virological Methods; 186; 1-2; 5-2012; 203-2060166-0934CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093412001656info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2012.05.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:31:51Zoai:ri.conicet.gov.ar:11336/199136instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:31:51.62CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR
title Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR
spellingShingle Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR
Pisano, María Belén
ALPHAVIRUS
ARBOVIRUS
DIAGNOSTIC
RT-NESTED PCR
SURVEILLANCE
VEEV
title_short Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR
title_full Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR
title_fullStr Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR
title_full_unstemmed Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR
title_sort Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR
dc.creator.none.fl_str_mv Pisano, María Belén
Sánchez Seco, María Paz
Ré, Viviana Elizabeth
Farías, Adrián A.
Contigiani, Marta Silvia
Tenorio, Antonio
author Pisano, María Belén
author_facet Pisano, María Belén
Sánchez Seco, María Paz
Ré, Viviana Elizabeth
Farías, Adrián A.
Contigiani, Marta Silvia
Tenorio, Antonio
author_role author
author2 Sánchez Seco, María Paz
Ré, Viviana Elizabeth
Farías, Adrián A.
Contigiani, Marta Silvia
Tenorio, Antonio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ALPHAVIRUS
ARBOVIRUS
DIAGNOSTIC
RT-NESTED PCR
SURVEILLANCE
VEEV
topic ALPHAVIRUS
ARBOVIRUS
DIAGNOSTIC
RT-NESTED PCR
SURVEILLANCE
VEEV
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance.
Fil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sánchez Seco, María Paz. Instituto de Salud Carlos III; España
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Farías, Adrián A.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Contigiani, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Tenorio, Antonio. Instituto de Salud Carlos III; España
description Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199136
Pisano, María Belén; Sánchez Seco, María Paz; Ré, Viviana Elizabeth; Farías, Adrián A.; Contigiani, Marta Silvia; et al.; Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR; Elsevier Science; Journal of Virological Methods; 186; 1-2; 5-2012; 203-206
0166-0934
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199136
identifier_str_mv Pisano, María Belén; Sánchez Seco, María Paz; Ré, Viviana Elizabeth; Farías, Adrián A.; Contigiani, Marta Silvia; et al.; Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR; Elsevier Science; Journal of Virological Methods; 186; 1-2; 5-2012; 203-206
0166-0934
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093412001656
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2012.05.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781926338199552
score 12.982451

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