Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR
- Autores
- Pisano, María Belén; Sánchez Seco, María Paz; Ré, Viviana Elizabeth; Farías, Adrián A.; Contigiani, Marta Silvia; Tenorio, Antonio
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance.
Fil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Sánchez Seco, María Paz. Instituto de Salud Carlos III; España
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Farías, Adrián A.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Contigiani, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Tenorio, Antonio. Instituto de Salud Carlos III; España - Materia
-
ALPHAVIRUS
ARBOVIRUS
DIAGNOSTIC
RT-NESTED PCR
SURVEILLANCE
VEEV - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/199136
Ver los metadatos del registro completo
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Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCRPisano, María BelénSánchez Seco, María PazRé, Viviana ElizabethFarías, Adrián A.Contigiani, Marta SilviaTenorio, AntonioALPHAVIRUSARBOVIRUSDIAGNOSTICRT-NESTED PCRSURVEILLANCEVEEVhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance.Fil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Sánchez Seco, María Paz. Instituto de Salud Carlos III; EspañaFil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Farías, Adrián A.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Contigiani, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Tenorio, Antonio. Instituto de Salud Carlos III; EspañaElsevier Science2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199136Pisano, María Belén; Sánchez Seco, María Paz; Ré, Viviana Elizabeth; Farías, Adrián A.; Contigiani, Marta Silvia; et al.; Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR; Elsevier Science; Journal of Virological Methods; 186; 1-2; 5-2012; 203-2060166-0934CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093412001656info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2012.05.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:23Zoai:ri.conicet.gov.ar:11336/199136instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:24.084CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR |
title |
Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR |
spellingShingle |
Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR Pisano, María Belén ALPHAVIRUS ARBOVIRUS DIAGNOSTIC RT-NESTED PCR SURVEILLANCE VEEV |
title_short |
Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR |
title_full |
Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR |
title_fullStr |
Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR |
title_full_unstemmed |
Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR |
title_sort |
Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR |
dc.creator.none.fl_str_mv |
Pisano, María Belén Sánchez Seco, María Paz Ré, Viviana Elizabeth Farías, Adrián A. Contigiani, Marta Silvia Tenorio, Antonio |
author |
Pisano, María Belén |
author_facet |
Pisano, María Belén Sánchez Seco, María Paz Ré, Viviana Elizabeth Farías, Adrián A. Contigiani, Marta Silvia Tenorio, Antonio |
author_role |
author |
author2 |
Sánchez Seco, María Paz Ré, Viviana Elizabeth Farías, Adrián A. Contigiani, Marta Silvia Tenorio, Antonio |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
ALPHAVIRUS ARBOVIRUS DIAGNOSTIC RT-NESTED PCR SURVEILLANCE VEEV |
topic |
ALPHAVIRUS ARBOVIRUS DIAGNOSTIC RT-NESTED PCR SURVEILLANCE VEEV |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance. Fil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Sánchez Seco, María Paz. Instituto de Salud Carlos III; España Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Farías, Adrián A.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina Fil: Contigiani, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina Fil: Tenorio, Antonio. Instituto de Salud Carlos III; España |
description |
Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/199136 Pisano, María Belén; Sánchez Seco, María Paz; Ré, Viviana Elizabeth; Farías, Adrián A.; Contigiani, Marta Silvia; et al.; Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR; Elsevier Science; Journal of Virological Methods; 186; 1-2; 5-2012; 203-206 0166-0934 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/199136 |
identifier_str_mv |
Pisano, María Belén; Sánchez Seco, María Paz; Ré, Viviana Elizabeth; Farías, Adrián A.; Contigiani, Marta Silvia; et al.; Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR; Elsevier Science; Journal of Virological Methods; 186; 1-2; 5-2012; 203-206 0166-0934 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093412001656 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2012.05.009 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613828034166784 |
score |
13.070432 |