Multimodal and non-linear optical microscopy applications in reproductive biology

Autores
Adur, Javier Fernando; Barbosa, G.O.; Pelegati, V. B.; Baratti, M. O.; Cesar, C. L.; Casco, Victor Hugo; Carvalho, H. F.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A plethora of optical techniques is currently available to obtain non-destructive, contactless, real time information with subcellular spatial resolution to observe cell processes. Each technique has its own unique features for imaging and for obtaining certain biological information. However none of the available techniques can be of universal use. For a comprehensive investigation of biological specimens and events, one needs to use a combination of bioimaging methods, often at the same time. Some modern confocal/multiphoton microscopes provide simultaneous fluorescence, fluorescence lifetime imaging, and four-dimensional imaging. Some of them can also easily be adapted for harmonic generation imaging, and to permit cell manipulation technique. In this work we present a multimodal optical workstation that extends a commercially available confocal microscope to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools. The nonlinear microscopy capabilities were added to the commercial confocal microscope by exploiting all the flexibility offered by the manufacturer. The various capabilities of this workstation as applied directly to reproductive biology are discussed.
Fil: Adur, Javier Fernando. Universidade Estadual de Campinas; Brasil. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina
Fil: Barbosa, G.O.. Universidade Estadual de Campinas; Brasil
Fil: Pelegati, V. B.. Universidade Estadual de Campinas; Brasil. Instituto Nacional de Ciencia y Tecnología sobre Fotónica Aplicada a la Biología Celular; Brasil
Fil: Baratti, M. O.. Universidade Estadual de Campinas; Brasil
Fil: Cesar, C. L.. Universidade Estadual de Campinas; Brasil. Universidade Estadual do Ceará; Brasil. Instituto Nacional de Ciencia y Tecnología sobre Fotónica Aplicada a la Biología Celular; Brasil
Fil: Casco, Victor Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Carvalho, H. F.. Instituto Nacional de Ciencia y Tecnología sobre Fotónica Aplicada a la Biología Celular; Brasil. Universidade Estadual de Campinas; Brasil
Materia
CONFOCAL PLATFORM
MULTIFOTON MICROSCOPY
NONLINEAR MICROSCOPY
OPTICAL WORKSTATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/106620
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multimodal and non-linear optical microscopy applications in reproductive biologyAdur, Javier FernandoBarbosa, G.O.Pelegati, V. B.Baratti, M. O.Cesar, C. L.Casco, Victor HugoCarvalho, H. F.CONFOCAL PLATFORMMULTIFOTON MICROSCOPYNONLINEAR MICROSCOPYOPTICAL WORKSTATIONhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1A plethora of optical techniques is currently available to obtain non-destructive, contactless, real time information with subcellular spatial resolution to observe cell processes. Each technique has its own unique features for imaging and for obtaining certain biological information. However none of the available techniques can be of universal use. For a comprehensive investigation of biological specimens and events, one needs to use a combination of bioimaging methods, often at the same time. Some modern confocal/multiphoton microscopes provide simultaneous fluorescence, fluorescence lifetime imaging, and four-dimensional imaging. Some of them can also easily be adapted for harmonic generation imaging, and to permit cell manipulation technique. In this work we present a multimodal optical workstation that extends a commercially available confocal microscope to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools. The nonlinear microscopy capabilities were added to the commercial confocal microscope by exploiting all the flexibility offered by the manufacturer. The various capabilities of this workstation as applied directly to reproductive biology are discussed.Fil: Adur, Javier Fernando. Universidade Estadual de Campinas; Brasil. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; ArgentinaFil: Barbosa, G.O.. Universidade Estadual de Campinas; BrasilFil: Pelegati, V. B.. Universidade Estadual de Campinas; Brasil. Instituto Nacional de Ciencia y Tecnología sobre Fotónica Aplicada a la Biología Celular; BrasilFil: Baratti, M. O.. Universidade Estadual de Campinas; BrasilFil: Cesar, C. L.. Universidade Estadual de Campinas; Brasil. Universidade Estadual do Ceará; Brasil. Instituto Nacional de Ciencia y Tecnología sobre Fotónica Aplicada a la Biología Celular; BrasilFil: Casco, Victor Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; ArgentinaFil: Carvalho, H. F.. Instituto Nacional de Ciencia y Tecnología sobre Fotónica Aplicada a la Biología Celular; Brasil. Universidade Estadual de Campinas; BrasilWiley2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/106620Adur, Javier Fernando; Barbosa, G.O.; Pelegati, V. B.; Baratti, M. O.; Cesar, C. L.; et al.; Multimodal and non-linear optical microscopy applications in reproductive biology; Wiley; Microscopy Research And Technique (online); 79; 7; 7-2016; 567-5821097-0029CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jemt.22684info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jemt.22684info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:42Zoai:ri.conicet.gov.ar:11336/106620instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:42.392CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multimodal and non-linear optical microscopy applications in reproductive biology
title Multimodal and non-linear optical microscopy applications in reproductive biology
spellingShingle Multimodal and non-linear optical microscopy applications in reproductive biology
Adur, Javier Fernando
CONFOCAL PLATFORM
MULTIFOTON MICROSCOPY
NONLINEAR MICROSCOPY
OPTICAL WORKSTATION
title_short Multimodal and non-linear optical microscopy applications in reproductive biology
title_full Multimodal and non-linear optical microscopy applications in reproductive biology
title_fullStr Multimodal and non-linear optical microscopy applications in reproductive biology
title_full_unstemmed Multimodal and non-linear optical microscopy applications in reproductive biology
title_sort Multimodal and non-linear optical microscopy applications in reproductive biology
dc.creator.none.fl_str_mv Adur, Javier Fernando
Barbosa, G.O.
Pelegati, V. B.
Baratti, M. O.
Cesar, C. L.
Casco, Victor Hugo
Carvalho, H. F.
author Adur, Javier Fernando
author_facet Adur, Javier Fernando
Barbosa, G.O.
Pelegati, V. B.
Baratti, M. O.
Cesar, C. L.
Casco, Victor Hugo
Carvalho, H. F.
author_role author
author2 Barbosa, G.O.
Pelegati, V. B.
Baratti, M. O.
Cesar, C. L.
Casco, Victor Hugo
Carvalho, H. F.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CONFOCAL PLATFORM
MULTIFOTON MICROSCOPY
NONLINEAR MICROSCOPY
OPTICAL WORKSTATION
topic CONFOCAL PLATFORM
MULTIFOTON MICROSCOPY
NONLINEAR MICROSCOPY
OPTICAL WORKSTATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A plethora of optical techniques is currently available to obtain non-destructive, contactless, real time information with subcellular spatial resolution to observe cell processes. Each technique has its own unique features for imaging and for obtaining certain biological information. However none of the available techniques can be of universal use. For a comprehensive investigation of biological specimens and events, one needs to use a combination of bioimaging methods, often at the same time. Some modern confocal/multiphoton microscopes provide simultaneous fluorescence, fluorescence lifetime imaging, and four-dimensional imaging. Some of them can also easily be adapted for harmonic generation imaging, and to permit cell manipulation technique. In this work we present a multimodal optical workstation that extends a commercially available confocal microscope to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools. The nonlinear microscopy capabilities were added to the commercial confocal microscope by exploiting all the flexibility offered by the manufacturer. The various capabilities of this workstation as applied directly to reproductive biology are discussed.
Fil: Adur, Javier Fernando. Universidade Estadual de Campinas; Brasil. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina
Fil: Barbosa, G.O.. Universidade Estadual de Campinas; Brasil
Fil: Pelegati, V. B.. Universidade Estadual de Campinas; Brasil. Instituto Nacional de Ciencia y Tecnología sobre Fotónica Aplicada a la Biología Celular; Brasil
Fil: Baratti, M. O.. Universidade Estadual de Campinas; Brasil
Fil: Cesar, C. L.. Universidade Estadual de Campinas; Brasil. Universidade Estadual do Ceará; Brasil. Instituto Nacional de Ciencia y Tecnología sobre Fotónica Aplicada a la Biología Celular; Brasil
Fil: Casco, Victor Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Carvalho, H. F.. Instituto Nacional de Ciencia y Tecnología sobre Fotónica Aplicada a la Biología Celular; Brasil. Universidade Estadual de Campinas; Brasil
description A plethora of optical techniques is currently available to obtain non-destructive, contactless, real time information with subcellular spatial resolution to observe cell processes. Each technique has its own unique features for imaging and for obtaining certain biological information. However none of the available techniques can be of universal use. For a comprehensive investigation of biological specimens and events, one needs to use a combination of bioimaging methods, often at the same time. Some modern confocal/multiphoton microscopes provide simultaneous fluorescence, fluorescence lifetime imaging, and four-dimensional imaging. Some of them can also easily be adapted for harmonic generation imaging, and to permit cell manipulation technique. In this work we present a multimodal optical workstation that extends a commercially available confocal microscope to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools. The nonlinear microscopy capabilities were added to the commercial confocal microscope by exploiting all the flexibility offered by the manufacturer. The various capabilities of this workstation as applied directly to reproductive biology are discussed.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/106620
Adur, Javier Fernando; Barbosa, G.O.; Pelegati, V. B.; Baratti, M. O.; Cesar, C. L.; et al.; Multimodal and non-linear optical microscopy applications in reproductive biology; Wiley; Microscopy Research And Technique (online); 79; 7; 7-2016; 567-582
1097-0029
CONICET Digital
CONICET
url http://hdl.handle.net/11336/106620
identifier_str_mv Adur, Javier Fernando; Barbosa, G.O.; Pelegati, V. B.; Baratti, M. O.; Cesar, C. L.; et al.; Multimodal and non-linear optical microscopy applications in reproductive biology; Wiley; Microscopy Research And Technique (online); 79; 7; 7-2016; 567-582
1097-0029
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jemt.22684
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jemt.22684
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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