Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy

Autores
Cattelan, Natalia; Yantorno, Osvaldo Miguel; Rajendar, Deora
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biofilms are sessile communities of microbial cells embedded in a self-produced or host-derived exopolymeric matrix. Biofilms can both be beneficial or detrimental depending on the surface. Compared to their planktonic counterparts, biofilm cells display enhanced resistance to killing by environmental threats, chemicals, antimicrobials and host immune defenses. When in biofilms, the microbial cells interact with each other and with the surface to develop architecturally complex multidimensional structures. Numerous imaging techniques and tools are currently available for architectural analyses of biofilm communities. This allows examination of biofilm development through acquisition of three-dimensional images that can render structural features of the sessile community. A frequently utilized tool is Confocal Laser Scanning Microscopy. We present a detailed protocol to grow, observe and analyze biofilms of the respiratory human pathogen, Bordetella pertussis in space and time.
Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Rajendar, Deora. Ohio State University; Estados Unidos
Materia
BIOFILM
CONFOCAL MICROSCOPY
BORDETELLA PERTUSSIS
WHOOPING COUGH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181317
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning MicroscopyCattelan, NataliaYantorno, Osvaldo MiguelRajendar, DeoraBIOFILMCONFOCAL MICROSCOPYBORDETELLA PERTUSSISWHOOPING COUGHhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Biofilms are sessile communities of microbial cells embedded in a self-produced or host-derived exopolymeric matrix. Biofilms can both be beneficial or detrimental depending on the surface. Compared to their planktonic counterparts, biofilm cells display enhanced resistance to killing by environmental threats, chemicals, antimicrobials and host immune defenses. When in biofilms, the microbial cells interact with each other and with the surface to develop architecturally complex multidimensional structures. Numerous imaging techniques and tools are currently available for architectural analyses of biofilm communities. This allows examination of biofilm development through acquisition of three-dimensional images that can render structural features of the sessile community. A frequently utilized tool is Confocal Laser Scanning Microscopy. We present a detailed protocol to grow, observe and analyze biofilms of the respiratory human pathogen, Bordetella pertussis in space and time.Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Rajendar, Deora. Ohio State University; Estados UnidosBio-Protocol Editors2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181317Cattelan, Natalia; Yantorno, Osvaldo Miguel; Rajendar, Deora; Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy; Bio-Protocol Editors; Bio-Protocol; 8; 15; 8-2018; 1-112331-8325CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bio-protocol.org/e2953info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.2953info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:05Zoai:ri.conicet.gov.ar:11336/181317instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:05.59CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy
title Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy
spellingShingle Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy
Cattelan, Natalia
BIOFILM
CONFOCAL MICROSCOPY
BORDETELLA PERTUSSIS
WHOOPING COUGH
title_short Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy
title_full Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy
title_fullStr Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy
title_full_unstemmed Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy
title_sort Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy
dc.creator.none.fl_str_mv Cattelan, Natalia
Yantorno, Osvaldo Miguel
Rajendar, Deora
author Cattelan, Natalia
author_facet Cattelan, Natalia
Yantorno, Osvaldo Miguel
Rajendar, Deora
author_role author
author2 Yantorno, Osvaldo Miguel
Rajendar, Deora
author2_role author
author
dc.subject.none.fl_str_mv BIOFILM
CONFOCAL MICROSCOPY
BORDETELLA PERTUSSIS
WHOOPING COUGH
topic BIOFILM
CONFOCAL MICROSCOPY
BORDETELLA PERTUSSIS
WHOOPING COUGH
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Biofilms are sessile communities of microbial cells embedded in a self-produced or host-derived exopolymeric matrix. Biofilms can both be beneficial or detrimental depending on the surface. Compared to their planktonic counterparts, biofilm cells display enhanced resistance to killing by environmental threats, chemicals, antimicrobials and host immune defenses. When in biofilms, the microbial cells interact with each other and with the surface to develop architecturally complex multidimensional structures. Numerous imaging techniques and tools are currently available for architectural analyses of biofilm communities. This allows examination of biofilm development through acquisition of three-dimensional images that can render structural features of the sessile community. A frequently utilized tool is Confocal Laser Scanning Microscopy. We present a detailed protocol to grow, observe and analyze biofilms of the respiratory human pathogen, Bordetella pertussis in space and time.
Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Rajendar, Deora. Ohio State University; Estados Unidos
description Biofilms are sessile communities of microbial cells embedded in a self-produced or host-derived exopolymeric matrix. Biofilms can both be beneficial or detrimental depending on the surface. Compared to their planktonic counterparts, biofilm cells display enhanced resistance to killing by environmental threats, chemicals, antimicrobials and host immune defenses. When in biofilms, the microbial cells interact with each other and with the surface to develop architecturally complex multidimensional structures. Numerous imaging techniques and tools are currently available for architectural analyses of biofilm communities. This allows examination of biofilm development through acquisition of three-dimensional images that can render structural features of the sessile community. A frequently utilized tool is Confocal Laser Scanning Microscopy. We present a detailed protocol to grow, observe and analyze biofilms of the respiratory human pathogen, Bordetella pertussis in space and time.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181317
Cattelan, Natalia; Yantorno, Osvaldo Miguel; Rajendar, Deora; Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy; Bio-Protocol Editors; Bio-Protocol; 8; 15; 8-2018; 1-11
2331-8325
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181317
identifier_str_mv Cattelan, Natalia; Yantorno, Osvaldo Miguel; Rajendar, Deora; Structural Analysis of Bordetella Pertussis Biofilms by Confocal Laser Scanning Microscopy; Bio-Protocol Editors; Bio-Protocol; 8; 15; 8-2018; 1-11
2331-8325
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bio-protocol.org/e2953
info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.2953
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bio-Protocol Editors
publisher.none.fl_str_mv Bio-Protocol Editors
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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