Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae)

Autores
Musin, Gabriela Eliana; Rossi, Andrea Silvana; Diawol, Valeria Paola; Collins, Pablo Agustin; Williner, Verónica
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The similarities or differences among species of the same family or among different families suggest a relationship with their feeding habits or a preference for the varios types of food. The aim of this study is to identify the presence of digestive enzymes lipases, total protease, amylases and cellulose, and the effects of sex and size in these activities in two decapod freshwater crustaceans. Specimens of different sizes and sexes of A. uruguayana and M. borellii were collected in the natural environment. In laboratory, enzymatic preparation consisted in homogenizing the digestive gland in a buffer Tris-HCl. The homogenates were centrifuged and the lipid layer was removed. Enzyme activities were determined by standard protocols. There were no statistically significant differences between sexes in any of the species under study. However, there were changes in the activity of amylases during the ontogeny of A. uruguayana, indicating that in juveniles the values of said activity are significantly higher than in the rest of the groups. M. borellii showed ontogenetic differences in total proteinase activity: organisms of range 2 (intermediate sizes) presented enzymatic activities much smaller than organisms of range 4 (larger sizes). The comparisons of enzymatic activities between both species yielded significant differences, being higher the values in the species A. uruguayana with respect to M. borellii. The results obtained in this work indicate that although both species are omnivorous and consume similar items, the activity of these digestive enzymes could be evidence of different strategies of utilization of the resource.
Fil: Musin, Gabriela Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Rossi, Andrea Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Diawol, Valeria Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Collins, Pablo Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Williner, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Materia
DISGESTION
HEPATOPANCREAS
JUVENILE
ADULTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88688
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/88688
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae)Musin, Gabriela ElianaRossi, Andrea SilvanaDiawol, Valeria PaolaCollins, Pablo AgustinWilliner, VerónicaDISGESTIONHEPATOPANCREASJUVENILEADULTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The similarities or differences among species of the same family or among different families suggest a relationship with their feeding habits or a preference for the varios types of food. The aim of this study is to identify the presence of digestive enzymes lipases, total protease, amylases and cellulose, and the effects of sex and size in these activities in two decapod freshwater crustaceans. Specimens of different sizes and sexes of A. uruguayana and M. borellii were collected in the natural environment. In laboratory, enzymatic preparation consisted in homogenizing the digestive gland in a buffer Tris-HCl. The homogenates were centrifuged and the lipid layer was removed. Enzyme activities were determined by standard protocols. There were no statistically significant differences between sexes in any of the species under study. However, there were changes in the activity of amylases during the ontogeny of A. uruguayana, indicating that in juveniles the values of said activity are significantly higher than in the rest of the groups. M. borellii showed ontogenetic differences in total proteinase activity: organisms of range 2 (intermediate sizes) presented enzymatic activities much smaller than organisms of range 4 (larger sizes). The comparisons of enzymatic activities between both species yielded significant differences, being higher the values in the species A. uruguayana with respect to M. borellii. The results obtained in this work indicate that although both species are omnivorous and consume similar items, the activity of these digestive enzymes could be evidence of different strategies of utilization of the resource.Fil: Musin, Gabriela Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Rossi, Andrea Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Diawol, Valeria Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Collins, Pablo Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Williner, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaWiley Blackwell Publishing, Inc2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88688Musin, Gabriela Eliana; Rossi, Andrea Silvana; Diawol, Valeria Paola; Collins, Pablo Agustin; Williner, Verónica; Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae); Wiley Blackwell Publishing, Inc; Aquaculture Research; 49; 12; 12-2018; 3889-38971355-557XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/are.13858info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:12Zoai:ri.conicet.gov.ar:11336/88688instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:13.139CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae)
title Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae)
spellingShingle Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae)
Musin, Gabriela Eliana
DISGESTION
HEPATOPANCREAS
JUVENILE
ADULTS
title_short Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae)
title_full Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae)
title_fullStr Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae)
title_full_unstemmed Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae)
title_sort Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae)
dc.creator.none.fl_str_mv Musin, Gabriela Eliana
Rossi, Andrea Silvana
Diawol, Valeria Paola
Collins, Pablo Agustin
Williner, Verónica
author Musin, Gabriela Eliana
author_facet Musin, Gabriela Eliana
Rossi, Andrea Silvana
Diawol, Valeria Paola
Collins, Pablo Agustin
Williner, Verónica
author_role author
author2 Rossi, Andrea Silvana
Diawol, Valeria Paola
Collins, Pablo Agustin
Williner, Verónica
author2_role author
author
author
author
dc.subject.none.fl_str_mv DISGESTION
HEPATOPANCREAS
JUVENILE
ADULTS
topic DISGESTION
HEPATOPANCREAS
JUVENILE
ADULTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The similarities or differences among species of the same family or among different families suggest a relationship with their feeding habits or a preference for the varios types of food. The aim of this study is to identify the presence of digestive enzymes lipases, total protease, amylases and cellulose, and the effects of sex and size in these activities in two decapod freshwater crustaceans. Specimens of different sizes and sexes of A. uruguayana and M. borellii were collected in the natural environment. In laboratory, enzymatic preparation consisted in homogenizing the digestive gland in a buffer Tris-HCl. The homogenates were centrifuged and the lipid layer was removed. Enzyme activities were determined by standard protocols. There were no statistically significant differences between sexes in any of the species under study. However, there were changes in the activity of amylases during the ontogeny of A. uruguayana, indicating that in juveniles the values of said activity are significantly higher than in the rest of the groups. M. borellii showed ontogenetic differences in total proteinase activity: organisms of range 2 (intermediate sizes) presented enzymatic activities much smaller than organisms of range 4 (larger sizes). The comparisons of enzymatic activities between both species yielded significant differences, being higher the values in the species A. uruguayana with respect to M. borellii. The results obtained in this work indicate that although both species are omnivorous and consume similar items, the activity of these digestive enzymes could be evidence of different strategies of utilization of the resource.
Fil: Musin, Gabriela Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Rossi, Andrea Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Diawol, Valeria Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Collins, Pablo Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Williner, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
description The similarities or differences among species of the same family or among different families suggest a relationship with their feeding habits or a preference for the varios types of food. The aim of this study is to identify the presence of digestive enzymes lipases, total protease, amylases and cellulose, and the effects of sex and size in these activities in two decapod freshwater crustaceans. Specimens of different sizes and sexes of A. uruguayana and M. borellii were collected in the natural environment. In laboratory, enzymatic preparation consisted in homogenizing the digestive gland in a buffer Tris-HCl. The homogenates were centrifuged and the lipid layer was removed. Enzyme activities were determined by standard protocols. There were no statistically significant differences between sexes in any of the species under study. However, there were changes in the activity of amylases during the ontogeny of A. uruguayana, indicating that in juveniles the values of said activity are significantly higher than in the rest of the groups. M. borellii showed ontogenetic differences in total proteinase activity: organisms of range 2 (intermediate sizes) presented enzymatic activities much smaller than organisms of range 4 (larger sizes). The comparisons of enzymatic activities between both species yielded significant differences, being higher the values in the species A. uruguayana with respect to M. borellii. The results obtained in this work indicate that although both species are omnivorous and consume similar items, the activity of these digestive enzymes could be evidence of different strategies of utilization of the resource.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88688
Musin, Gabriela Eliana; Rossi, Andrea Silvana; Diawol, Valeria Paola; Collins, Pablo Agustin; Williner, Verónica; Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae); Wiley Blackwell Publishing, Inc; Aquaculture Research; 49; 12; 12-2018; 3889-3897
1355-557X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88688
identifier_str_mv Musin, Gabriela Eliana; Rossi, Andrea Silvana; Diawol, Valeria Paola; Collins, Pablo Agustin; Williner, Verónica; Development of enzymes during ontogeny of two freshwater Decapoda: Aegla uruguayana (Aeglidae) and Macrobrachium borellii (Palaemonidae); Wiley Blackwell Publishing, Inc; Aquaculture Research; 49; 12; 12-2018; 3889-3897
1355-557X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/are.13858
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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