Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis

Autores
Soto Vargas, Verónica Carolina; Fernández, María de Los Ángeles; Galmarini, Claudio Romulo; Silva, María Fernanda
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L-1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production.
Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
Onion Nectar
Spe
Capillary Electrophoresis
Phenolic Compounds
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38965
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38965
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresisSoto Vargas, Verónica CarolinaFernández, María de Los ÁngelesGalmarini, Claudio RomuloSilva, María FernandaOnion NectarSpeCapillary ElectrophoresisPhenolic Compoundshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L-1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production.Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaFil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaRoyal Society of Chemistry2014-07-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38965Soto Vargas, Verónica Carolina; Fernández, María de Los Ángeles; Galmarini, Claudio Romulo; Silva, María Fernanda; Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis; Royal Society of Chemistry; Analytical Methods; 6; 13; 16-7-2014; 4878-48841759-9660CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/c4ay00240ginfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/C4AY00240G#!divAbstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:46Zoai:ri.conicet.gov.ar:11336/38965instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:46.317CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
title Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
spellingShingle Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
Soto Vargas, Verónica Carolina
Onion Nectar
Spe
Capillary Electrophoresis
Phenolic Compounds
title_short Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
title_full Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
title_fullStr Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
title_full_unstemmed Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
title_sort Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
dc.creator.none.fl_str_mv Soto Vargas, Verónica Carolina
Fernández, María de Los Ángeles
Galmarini, Claudio Romulo
Silva, María Fernanda
author Soto Vargas, Verónica Carolina
author_facet Soto Vargas, Verónica Carolina
Fernández, María de Los Ángeles
Galmarini, Claudio Romulo
Silva, María Fernanda
author_role author
author2 Fernández, María de Los Ángeles
Galmarini, Claudio Romulo
Silva, María Fernanda
author2_role author
author
author
dc.subject.none.fl_str_mv Onion Nectar
Spe
Capillary Electrophoresis
Phenolic Compounds
topic Onion Nectar
Spe
Capillary Electrophoresis
Phenolic Compounds
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L-1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production.
Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L-1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production.
publishDate 2014
dc.date.none.fl_str_mv 2014-07-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38965
Soto Vargas, Verónica Carolina; Fernández, María de Los Ángeles; Galmarini, Claudio Romulo; Silva, María Fernanda; Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis; Royal Society of Chemistry; Analytical Methods; 6; 13; 16-7-2014; 4878-4884
1759-9660
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38965
identifier_str_mv Soto Vargas, Verónica Carolina; Fernández, María de Los Ángeles; Galmarini, Claudio Romulo; Silva, María Fernanda; Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis; Royal Society of Chemistry; Analytical Methods; 6; 13; 16-7-2014; 4878-4884
1759-9660
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/c4ay00240g
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/C4AY00240G#!divAbstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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