Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
- Autores
- Soto Vargas, Verónica Carolina; Fernández, María de Los Ángeles; Galmarini, Claudio Romulo; Silva, María Fernanda
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L−1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production.
EEA La Consulta
Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina - Fuente
- Analytical methods 13 : 4878–4884. (2014)
- Materia
-
Compuestos Fenólicos
Néctar
Cebolla
Allium Cepa
Electroforesis
Phenolic Compounds
Onions
Electrophoresis
Capillary Electrophoresis
Solid Phase Extraction
SPE - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/3905
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Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresisSoto Vargas, Verónica CarolinaFernández, María de Los ÁngelesGalmarini, Claudio RomuloSilva, María FernandaCompuestos FenólicosNéctarCebollaAllium CepaElectroforesisPhenolic CompoundsOnionsElectrophoresisCapillary ElectrophoresisSolid Phase ExtractionSPEOnion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L−1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production.EEA La ConsultaFil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaFil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaRoyal Society of Chemistry2018-11-15T14:41:54Z2018-11-15T14:41:54Z2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3905https://pubs.rsc.org/en/content/articlelanding/2014/ay/c4ay00240g#!divAbstract1759-967910.1039/c4ay00240gAnalytical methods 13 : 4878–4884. (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:22Zoai:localhost:20.500.12123/3905instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:22.936INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
title |
Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
spellingShingle |
Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis Soto Vargas, Verónica Carolina Compuestos Fenólicos Néctar Cebolla Allium Cepa Electroforesis Phenolic Compounds Onions Electrophoresis Capillary Electrophoresis Solid Phase Extraction SPE |
title_short |
Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
title_full |
Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
title_fullStr |
Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
title_full_unstemmed |
Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
title_sort |
Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
dc.creator.none.fl_str_mv |
Soto Vargas, Verónica Carolina Fernández, María de Los Ángeles Galmarini, Claudio Romulo Silva, María Fernanda |
author |
Soto Vargas, Verónica Carolina |
author_facet |
Soto Vargas, Verónica Carolina Fernández, María de Los Ángeles Galmarini, Claudio Romulo Silva, María Fernanda |
author_role |
author |
author2 |
Fernández, María de Los Ángeles Galmarini, Claudio Romulo Silva, María Fernanda |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Compuestos Fenólicos Néctar Cebolla Allium Cepa Electroforesis Phenolic Compounds Onions Electrophoresis Capillary Electrophoresis Solid Phase Extraction SPE |
topic |
Compuestos Fenólicos Néctar Cebolla Allium Cepa Electroforesis Phenolic Compounds Onions Electrophoresis Capillary Electrophoresis Solid Phase Extraction SPE |
dc.description.none.fl_txt_mv |
Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L−1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production. EEA La Consulta Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina |
description |
Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L−1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2018-11-15T14:41:54Z 2018-11-15T14:41:54Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/3905 https://pubs.rsc.org/en/content/articlelanding/2014/ay/c4ay00240g#!divAbstract 1759-9679 10.1039/c4ay00240g |
url |
http://hdl.handle.net/20.500.12123/3905 https://pubs.rsc.org/en/content/articlelanding/2014/ay/c4ay00240g#!divAbstract |
identifier_str_mv |
1759-9679 10.1039/c4ay00240g |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
Analytical methods 13 : 4878–4884. (2014) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1846143508197408768 |
score |
12.712165 |