Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis

Autores
Soto Vargas, Verónica Carolina; Fernández, María de Los Ángeles; Galmarini, Claudio Romulo; Silva, María Fernanda
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L−1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production.
EEA La Consulta
Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fuente
Analytical methods 13 : 4878–4884. (2014)
Materia
Compuestos Fenólicos
Néctar
Cebolla
Allium Cepa
Electroforesis
Phenolic Compounds
Onions
Electrophoresis
Capillary Electrophoresis
Solid Phase Extraction
SPE
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3905

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oai_identifier_str oai:localhost:20.500.12123/3905
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network_name_str INTA Digital (INTA)
spelling Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresisSoto Vargas, Verónica CarolinaFernández, María de Los ÁngelesGalmarini, Claudio RomuloSilva, María FernandaCompuestos FenólicosNéctarCebollaAllium CepaElectroforesisPhenolic CompoundsOnionsElectrophoresisCapillary ElectrophoresisSolid Phase ExtractionSPEOnion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L−1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production.EEA La ConsultaFil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaFil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaRoyal Society of Chemistry2018-11-15T14:41:54Z2018-11-15T14:41:54Z2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3905https://pubs.rsc.org/en/content/articlelanding/2014/ay/c4ay00240g#!divAbstract1759-967910.1039/c4ay00240gAnalytical methods 13 : 4878–4884. (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:22Zoai:localhost:20.500.12123/3905instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:22.936INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
title Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
spellingShingle Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
Soto Vargas, Verónica Carolina
Compuestos Fenólicos
Néctar
Cebolla
Allium Cepa
Electroforesis
Phenolic Compounds
Onions
Electrophoresis
Capillary Electrophoresis
Solid Phase Extraction
SPE
title_short Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
title_full Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
title_fullStr Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
title_full_unstemmed Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
title_sort Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
dc.creator.none.fl_str_mv Soto Vargas, Verónica Carolina
Fernández, María de Los Ángeles
Galmarini, Claudio Romulo
Silva, María Fernanda
author Soto Vargas, Verónica Carolina
author_facet Soto Vargas, Verónica Carolina
Fernández, María de Los Ángeles
Galmarini, Claudio Romulo
Silva, María Fernanda
author_role author
author2 Fernández, María de Los Ángeles
Galmarini, Claudio Romulo
Silva, María Fernanda
author2_role author
author
author
dc.subject.none.fl_str_mv Compuestos Fenólicos
Néctar
Cebolla
Allium Cepa
Electroforesis
Phenolic Compounds
Onions
Electrophoresis
Capillary Electrophoresis
Solid Phase Extraction
SPE
topic Compuestos Fenólicos
Néctar
Cebolla
Allium Cepa
Electroforesis
Phenolic Compounds
Onions
Electrophoresis
Capillary Electrophoresis
Solid Phase Extraction
SPE
dc.description.none.fl_txt_mv Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L−1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production.
EEA La Consulta
Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L−1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production.
publishDate 2014
dc.date.none.fl_str_mv 2014
2018-11-15T14:41:54Z
2018-11-15T14:41:54Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3905
https://pubs.rsc.org/en/content/articlelanding/2014/ay/c4ay00240g#!divAbstract
1759-9679
10.1039/c4ay00240g
url http://hdl.handle.net/20.500.12123/3905
https://pubs.rsc.org/en/content/articlelanding/2014/ay/c4ay00240g#!divAbstract
identifier_str_mv 1759-9679
10.1039/c4ay00240g
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv Analytical methods 13 : 4878–4884. (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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