Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota

Autores
Fernandez Gnecco, Gabriela Amancay; Gégu, Louis; Covacevich, Fernanda; Consolo, Verónica Fabiana; Bouffaud, Marie Lara; Buscot, François; Smalla, Kornelia; Babin, Doreen
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
he objective of the present study wasto evaluate the effects of single or combined inoculation of Trichoderma harzianum,strain TGFG411, and a consortium of arbuscular mycorrhizal fungi (AMF) on plantgrowth, and native microbial communities (here bacteria/archaea, fungi and AMF) inroot‐associated soil (RAS) and rhizosphere (RH), that is, soil loosely or tightly attached tothe roots, respectively.Materials and Methods: A greenhouse experiment was carried out with non‐sterileagricultural soil and the model crop maize, which was single inoculated with eitherTGFG411 or AMF or received a combined inoculation of TGFG411 + AMF. Controlplants received only water. Seven weeks after the second AMF inoculation, the plantgrowth promotion capacity of the inoculants was measured based on shoot and rootparameters. Furthermore, RAS and RH microbiota (fungi including AMF, bacteria andarchaea) were assessed via a combination of different cultivation‐dependent,microscopic and DNA‐based methods.Results: After 7 weeks of maize growth, both single and combined inoculation of AMFand TGFG411 enhanced shoot dry weight and led to a significant reduction in rootbiomass. The TGFG411 strain successfully established in the soil. However, no definiteevidence for the establishment of the inoculated AMF was found. Single or combinedinoculation of TGFG411 and AMF modified the composition of total bacterial in the RH,whereas modulated total fungal communities in the RAS.Conclusion: The combined inoculation did not result in a significant improvement ofplant performance compared with single inoculation likely due to optimal nutrientsupply. However, samples receiving the combined inoculation exhibited a distinct modulation of the native RAS/RH microbiota, which may influence the inoculantefficacy under less favourable conditions.
Fil: Fernandez Gnecco, Gabriela Amancay. Helmholtz Centre for Environmental Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Gégu, Louis. Helmholtz Centre for Environmental Research; Alemania
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Consolo, Verónica Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Bouffaud, Marie Lara. Helmholtz Centre for Environmental Research; Alemania
Fil: Buscot, François. Helmholtz Centre for Environmental Research; Alemania
Fil: Smalla, Kornelia. Helmholtz Centre for Environmental Research; Alemania
Fil: Babin, Doreen. Helmholtz Centre for Environmental Research; Alemania
Materia
dual inoculation
plant growth promoting microorganism
zea mays
pot experiment
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281068
Acceder
id CONICETDig_1ea47507dc3d9fb3e7b72889a0bd67db
oai_identifier_str oai:ri.conicet.gov.ar:11336/281068
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiotaFernandez Gnecco, Gabriela AmancayGégu, LouisCovacevich, FernandaConsolo, Verónica FabianaBouffaud, Marie LaraBuscot, FrançoisSmalla, KorneliaBabin, Doreendual inoculationplant growth promoting microorganismzea mayspot experimenthttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4he objective of the present study wasto evaluate the effects of single or combined inoculation of Trichoderma harzianum,strain TGFG411, and a consortium of arbuscular mycorrhizal fungi (AMF) on plantgrowth, and native microbial communities (here bacteria/archaea, fungi and AMF) inroot‐associated soil (RAS) and rhizosphere (RH), that is, soil loosely or tightly attached tothe roots, respectively.Materials and Methods: A greenhouse experiment was carried out with non‐sterileagricultural soil and the model crop maize, which was single inoculated with eitherTGFG411 or AMF or received a combined inoculation of TGFG411 + AMF. Controlplants received only water. Seven weeks after the second AMF inoculation, the plantgrowth promotion capacity of the inoculants was measured based on shoot and rootparameters. Furthermore, RAS and RH microbiota (fungi including AMF, bacteria andarchaea) were assessed via a combination of different cultivation‐dependent,microscopic and DNA‐based methods.Results: After 7 weeks of maize growth, both single and combined inoculation of AMFand TGFG411 enhanced shoot dry weight and led to a significant reduction in rootbiomass. The TGFG411 strain successfully established in the soil. However, no definiteevidence for the establishment of the inoculated AMF was found. Single or combinedinoculation of TGFG411 and AMF modified the composition of total bacterial in the RH,whereas modulated total fungal communities in the RAS.Conclusion: The combined inoculation did not result in a significant improvement ofplant performance compared with single inoculation likely due to optimal nutrientsupply. However, samples receiving the combined inoculation exhibited a distinct modulation of the native RAS/RH microbiota, which may influence the inoculantefficacy under less favourable conditions.Fil: Fernandez Gnecco, Gabriela Amancay. Helmholtz Centre for Environmental Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Gégu, Louis. Helmholtz Centre for Environmental Research; AlemaniaFil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Consolo, Verónica Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Bouffaud, Marie Lara. Helmholtz Centre for Environmental Research; AlemaniaFil: Buscot, François. Helmholtz Centre for Environmental Research; AlemaniaFil: Smalla, Kornelia. Helmholtz Centre for Environmental Research; AlemaniaFil: Babin, Doreen. Helmholtz Centre for Environmental Research; AlemaniaWiley2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281068Fernandez Gnecco, Gabriela Amancay; Gégu, Louis; Covacevich, Fernanda; Consolo, Verónica Fabiana; Bouffaud, Marie Lara; et al.; Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota; Wiley; Journal of Sustainable Agriculture and Environment; 3; 1; 1-2024; 1-162767-035X2767-035XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/sae2.12091info:eu-repo/semantics/altIdentifier/doi/10.1002/sae2.12091info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:26:04Zoai:ri.conicet.gov.ar:11336/281068instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:26:04.474CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota
title Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota
spellingShingle Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota
Fernandez Gnecco, Gabriela Amancay
dual inoculation
plant growth promoting microorganism
zea mays
pot experiment
title_short Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota
title_full Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota
title_fullStr Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota
title_full_unstemmed Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota
title_sort Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota
dc.creator.none.fl_str_mv Fernandez Gnecco, Gabriela Amancay
Gégu, Louis
Covacevich, Fernanda
Consolo, Verónica Fabiana
Bouffaud, Marie Lara
Buscot, François
Smalla, Kornelia
Babin, Doreen
author Fernandez Gnecco, Gabriela Amancay
author_facet Fernandez Gnecco, Gabriela Amancay
Gégu, Louis
Covacevich, Fernanda
Consolo, Verónica Fabiana
Bouffaud, Marie Lara
Buscot, François
Smalla, Kornelia
Babin, Doreen
author_role author
author2 Gégu, Louis
Covacevich, Fernanda
Consolo, Verónica Fabiana
Bouffaud, Marie Lara
Buscot, François
Smalla, Kornelia
Babin, Doreen
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv dual inoculation
plant growth promoting microorganism
zea mays
pot experiment
topic dual inoculation
plant growth promoting microorganism
zea mays
pot experiment
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv he objective of the present study wasto evaluate the effects of single or combined inoculation of Trichoderma harzianum,strain TGFG411, and a consortium of arbuscular mycorrhizal fungi (AMF) on plantgrowth, and native microbial communities (here bacteria/archaea, fungi and AMF) inroot‐associated soil (RAS) and rhizosphere (RH), that is, soil loosely or tightly attached tothe roots, respectively.Materials and Methods: A greenhouse experiment was carried out with non‐sterileagricultural soil and the model crop maize, which was single inoculated with eitherTGFG411 or AMF or received a combined inoculation of TGFG411 + AMF. Controlplants received only water. Seven weeks after the second AMF inoculation, the plantgrowth promotion capacity of the inoculants was measured based on shoot and rootparameters. Furthermore, RAS and RH microbiota (fungi including AMF, bacteria andarchaea) were assessed via a combination of different cultivation‐dependent,microscopic and DNA‐based methods.Results: After 7 weeks of maize growth, both single and combined inoculation of AMFand TGFG411 enhanced shoot dry weight and led to a significant reduction in rootbiomass. The TGFG411 strain successfully established in the soil. However, no definiteevidence for the establishment of the inoculated AMF was found. Single or combinedinoculation of TGFG411 and AMF modified the composition of total bacterial in the RH,whereas modulated total fungal communities in the RAS.Conclusion: The combined inoculation did not result in a significant improvement ofplant performance compared with single inoculation likely due to optimal nutrientsupply. However, samples receiving the combined inoculation exhibited a distinct modulation of the native RAS/RH microbiota, which may influence the inoculantefficacy under less favourable conditions.
Fil: Fernandez Gnecco, Gabriela Amancay. Helmholtz Centre for Environmental Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Gégu, Louis. Helmholtz Centre for Environmental Research; Alemania
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Consolo, Verónica Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Bouffaud, Marie Lara. Helmholtz Centre for Environmental Research; Alemania
Fil: Buscot, François. Helmholtz Centre for Environmental Research; Alemania
Fil: Smalla, Kornelia. Helmholtz Centre for Environmental Research; Alemania
Fil: Babin, Doreen. Helmholtz Centre for Environmental Research; Alemania
description he objective of the present study wasto evaluate the effects of single or combined inoculation of Trichoderma harzianum,strain TGFG411, and a consortium of arbuscular mycorrhizal fungi (AMF) on plantgrowth, and native microbial communities (here bacteria/archaea, fungi and AMF) inroot‐associated soil (RAS) and rhizosphere (RH), that is, soil loosely or tightly attached tothe roots, respectively.Materials and Methods: A greenhouse experiment was carried out with non‐sterileagricultural soil and the model crop maize, which was single inoculated with eitherTGFG411 or AMF or received a combined inoculation of TGFG411 + AMF. Controlplants received only water. Seven weeks after the second AMF inoculation, the plantgrowth promotion capacity of the inoculants was measured based on shoot and rootparameters. Furthermore, RAS and RH microbiota (fungi including AMF, bacteria andarchaea) were assessed via a combination of different cultivation‐dependent,microscopic and DNA‐based methods.Results: After 7 weeks of maize growth, both single and combined inoculation of AMFand TGFG411 enhanced shoot dry weight and led to a significant reduction in rootbiomass. The TGFG411 strain successfully established in the soil. However, no definiteevidence for the establishment of the inoculated AMF was found. Single or combinedinoculation of TGFG411 and AMF modified the composition of total bacterial in the RH,whereas modulated total fungal communities in the RAS.Conclusion: The combined inoculation did not result in a significant improvement ofplant performance compared with single inoculation likely due to optimal nutrientsupply. However, samples receiving the combined inoculation exhibited a distinct modulation of the native RAS/RH microbiota, which may influence the inoculantefficacy under less favourable conditions.
publishDate 2024
dc.date.none.fl_str_mv 2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281068
Fernandez Gnecco, Gabriela Amancay; Gégu, Louis; Covacevich, Fernanda; Consolo, Verónica Fabiana; Bouffaud, Marie Lara; et al.; Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota; Wiley; Journal of Sustainable Agriculture and Environment; 3; 1; 1-2024; 1-16
2767-035X
2767-035X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281068
identifier_str_mv Fernandez Gnecco, Gabriela Amancay; Gégu, Louis; Covacevich, Fernanda; Consolo, Verónica Fabiana; Bouffaud, Marie Lara; et al.; Alone as effective as together: AMF and Trichoderma inoculation boost maize performance but differentially shape soil and rhizosphere microbiota; Wiley; Journal of Sustainable Agriculture and Environment; 3; 1; 1-2024; 1-16
2767-035X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/sae2.12091
info:eu-repo/semantics/altIdentifier/doi/10.1002/sae2.12091
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.665996

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