Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity

Autores
Covacevich, Fernanda; Fernandez Gnecco, Gabriela Amancay; Consolo, Verónica; Burges, Pablo; Calo, Gonzalo; Mondino, Eduardo Ariel
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Arbuscular mycorrhizal fungi (AMF) and Trichoderma spp. (T) are known as plant-beneficial fungi effective against root-knot nematodes, but their interactions in the rhizosphere are not well understood. This study examined how Meloidogyne javanica influences AMF colonization and community diversity at the root-soil interface of tomato plants. A 60-day growth chamber experiment was conducted with tomato plants grown in non-sterile agricultural soil, either infected or not with M. javanica, that received a single inoculation with AMF or Trichoderma (strains T363 or TJ15), combined AMF + T inoculations, or no inoculation (Control). Both single and combined inoculations significantly reduced root galls, eggs, and soil nematode larvae. An AMF community analysis via single-strand conformation polymorphism of the D1 region of 28S rDNA gene (Glomeraceae family) revealed that M. javanica decreased AMF diversity and altered community structure, in plants single-inoculated with AMF. However, a combined inoculation with Trichoderma appears to prevent this reduction and maintain AMF diversity. While M. javanica reduced root mycorrhizal colonization, it did not affect Trichoderma abundance. These results suggest that Trichoderma may be more resilient to nematode infection, helping stabilize AMF communities and enhance biocontrol. Thus, combining AMF and Trichoderma inoculations could better preserve root health and improve biological control effectiveness against M. javanica.
EEA Balcarce
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina
Fil: Fernandez Gnecco, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina
Fil: Consolo, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina
Fil: Burges, Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Calo, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina
Fil: Mondino, Eduardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fuente
Diversity 17 (5) : 334 (May 2025)
Materia
Biodiversidad
Suelo
Nematodos
Parásitos
Inoculación
Control Biológico
Biodiversity
Soil
Nematodes
Parasites
Mycorrhizae
Trichoderma
Inoculation
Biological Control
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22672
Acceder
id INTADig_9465945aa6b4f92563ba48aac79dbfec
oai_identifier_str oai:localhost:20.500.12123/22672
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal DiversityCovacevich, FernandaFernandez Gnecco, Gabriela AmancayConsolo, VerónicaBurges, PabloCalo, GonzaloMondino, Eduardo ArielBiodiversidadSueloNematodosParásitosInoculaciónControl BiológicoBiodiversitySoilNematodesParasitesMycorrhizaeTrichodermaInoculationBiological ControlArbuscular mycorrhizal fungi (AMF) and Trichoderma spp. (T) are known as plant-beneficial fungi effective against root-knot nematodes, but their interactions in the rhizosphere are not well understood. This study examined how Meloidogyne javanica influences AMF colonization and community diversity at the root-soil interface of tomato plants. A 60-day growth chamber experiment was conducted with tomato plants grown in non-sterile agricultural soil, either infected or not with M. javanica, that received a single inoculation with AMF or Trichoderma (strains T363 or TJ15), combined AMF + T inoculations, or no inoculation (Control). Both single and combined inoculations significantly reduced root galls, eggs, and soil nematode larvae. An AMF community analysis via single-strand conformation polymorphism of the D1 region of 28S rDNA gene (Glomeraceae family) revealed that M. javanica decreased AMF diversity and altered community structure, in plants single-inoculated with AMF. However, a combined inoculation with Trichoderma appears to prevent this reduction and maintain AMF diversity. While M. javanica reduced root mycorrhizal colonization, it did not affect Trichoderma abundance. These results suggest that Trichoderma may be more resilient to nematode infection, helping stabilize AMF communities and enhance biocontrol. Thus, combining AMF and Trichoderma inoculations could better preserve root health and improve biological control effectiveness against M. javanica.EEA BalcarceFil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; ArgentinaFil: Fernandez Gnecco, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; ArgentinaFil: Consolo, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; ArgentinaFil: Burges, Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Calo, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; ArgentinaFil: Mondino, Eduardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaMDPI2025-06-13T10:01:27Z2025-06-13T10:01:27Z2025-05-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22672https://www.mdpi.com/1424-2818/17/5/3341424-2818https://doi.org/10.3390/d17050334Diversity 17 (5) : 334 (May 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L03-I093, Fertilidad química, física y biológica del suelo: estrategias para mitigar y restaurar procesos de degradacióninfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:07Zoai:localhost:20.500.12123/22672instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:07.688INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity
title Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity
spellingShingle Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity
Covacevich, Fernanda
Biodiversidad
Suelo
Nematodos
Parásitos
Inoculación
Control Biológico
Biodiversity
Soil
Nematodes
Parasites
Mycorrhizae
Trichoderma
Inoculation
Biological Control
title_short Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity
title_full Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity
title_fullStr Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity
title_full_unstemmed Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity
title_sort Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity
dc.creator.none.fl_str_mv Covacevich, Fernanda
Fernandez Gnecco, Gabriela Amancay
Consolo, Verónica
Burges, Pablo
Calo, Gonzalo
Mondino, Eduardo Ariel
author Covacevich, Fernanda
author_facet Covacevich, Fernanda
Fernandez Gnecco, Gabriela Amancay
Consolo, Verónica
Burges, Pablo
Calo, Gonzalo
Mondino, Eduardo Ariel
author_role author
author2 Fernandez Gnecco, Gabriela Amancay
Consolo, Verónica
Burges, Pablo
Calo, Gonzalo
Mondino, Eduardo Ariel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biodiversidad
Suelo
Nematodos
Parásitos
Inoculación
Control Biológico
Biodiversity
Soil
Nematodes
Parasites
Mycorrhizae
Trichoderma
Inoculation
Biological Control
topic Biodiversidad
Suelo
Nematodos
Parásitos
Inoculación
Control Biológico
Biodiversity
Soil
Nematodes
Parasites
Mycorrhizae
Trichoderma
Inoculation
Biological Control
dc.description.none.fl_txt_mv Arbuscular mycorrhizal fungi (AMF) and Trichoderma spp. (T) are known as plant-beneficial fungi effective against root-knot nematodes, but their interactions in the rhizosphere are not well understood. This study examined how Meloidogyne javanica influences AMF colonization and community diversity at the root-soil interface of tomato plants. A 60-day growth chamber experiment was conducted with tomato plants grown in non-sterile agricultural soil, either infected or not with M. javanica, that received a single inoculation with AMF or Trichoderma (strains T363 or TJ15), combined AMF + T inoculations, or no inoculation (Control). Both single and combined inoculations significantly reduced root galls, eggs, and soil nematode larvae. An AMF community analysis via single-strand conformation polymorphism of the D1 region of 28S rDNA gene (Glomeraceae family) revealed that M. javanica decreased AMF diversity and altered community structure, in plants single-inoculated with AMF. However, a combined inoculation with Trichoderma appears to prevent this reduction and maintain AMF diversity. While M. javanica reduced root mycorrhizal colonization, it did not affect Trichoderma abundance. These results suggest that Trichoderma may be more resilient to nematode infection, helping stabilize AMF communities and enhance biocontrol. Thus, combining AMF and Trichoderma inoculations could better preserve root health and improve biological control effectiveness against M. javanica.
EEA Balcarce
Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina
Fil: Fernandez Gnecco, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina
Fil: Consolo, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina
Fil: Burges, Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Calo, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina
Fil: Mondino, Eduardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
description Arbuscular mycorrhizal fungi (AMF) and Trichoderma spp. (T) are known as plant-beneficial fungi effective against root-knot nematodes, but their interactions in the rhizosphere are not well understood. This study examined how Meloidogyne javanica influences AMF colonization and community diversity at the root-soil interface of tomato plants. A 60-day growth chamber experiment was conducted with tomato plants grown in non-sterile agricultural soil, either infected or not with M. javanica, that received a single inoculation with AMF or Trichoderma (strains T363 or TJ15), combined AMF + T inoculations, or no inoculation (Control). Both single and combined inoculations significantly reduced root galls, eggs, and soil nematode larvae. An AMF community analysis via single-strand conformation polymorphism of the D1 region of 28S rDNA gene (Glomeraceae family) revealed that M. javanica decreased AMF diversity and altered community structure, in plants single-inoculated with AMF. However, a combined inoculation with Trichoderma appears to prevent this reduction and maintain AMF diversity. While M. javanica reduced root mycorrhizal colonization, it did not affect Trichoderma abundance. These results suggest that Trichoderma may be more resilient to nematode infection, helping stabilize AMF communities and enhance biocontrol. Thus, combining AMF and Trichoderma inoculations could better preserve root health and improve biological control effectiveness against M. javanica.
publishDate 2025
dc.date.none.fl_str_mv 2025-06-13T10:01:27Z
2025-06-13T10:01:27Z
2025-05-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22672
https://www.mdpi.com/1424-2818/17/5/334
1424-2818
https://doi.org/10.3390/d17050334
url http://hdl.handle.net/20.500.12123/22672
https://www.mdpi.com/1424-2818/17/5/334
https://doi.org/10.3390/d17050334
identifier_str_mv 1424-2818
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L03-I093, Fertilidad química, física y biológica del suelo: estrategias para mitigar y restaurar procesos de degradación
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Diversity 17 (5) : 334 (May 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341441499561984
score 12.623145

Publicaciones similares