Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland

Autores
Moré, Gastón Andrés; Carlotta, Filippini; Oehm, Andreas W.; Maja, Ruetten; Andrew, Hemphill; Frey, Caroline F.; Basso, Walter Ubaldo
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sarcocystis spp. and Toxoplasma gondii are heteroxenous protozoan parasites, which form cysts in muscle tissue of intermediate hosts (IH) and oocysts in the intestinal mucosa of definitive hosts (DH). Wild boars (Sus scrofa) are IH for Sarcocystis miescheriana (with wild and domestic canids as DH), S. suihominis (with humans and nonhuman primates as DH) and T. gondii (with felids as DH). The aim of this study was to identify and characterize Sarcocystis spp. and T. gondii in the muscle tissue of wild boars hunted and/or consumed in Switzerland. DNA was extracted from muscle samples of 286 wild boars and tested by PCRs targeting the 18S rRNA gene of Sarcocystis spp., the mitochondrial cytochrome c oxidase (cox1) gene of S. suihominis and by a qPCR targeting a 529 bp genomic repeat of T. gondii. Besides, 225 samples were processed by homogenization and direct microscopic examination and 42 samples by histopathology. Sarcocysts were detected microscopically in 89.3 % (201/225) and 35.7 % (15/42) of the samples, respectively. The 18S rRNA PCR detected parasite DNA in 91.3 % (261/286) of the samples, and all 62 sequences obtained were 100 % identical, and also with GenBank sequences reported as S. miescheriana, suggesting a high environmental contamination with canid faeces. The S. suihominiscox1 PCR was positive in 1.75 % of the samples (5/286), and the six cox1 sequences (772 bp primers trimmed) obtained were 97.3–99.6 % similar among them and 97.7–100 % identical with a sequence reported as S. suihominis. Despite the identity differences, all these sequences were grouped together in a phylogenetic tree, in a sister clade of S. miescheriana sequences. All samples tested negative for T. gondii by qPCR. This study revealed for the first time the presence of S. suihominis in wild boars hunted in Switzerland.
Fil: Moré, Gastón Andrés. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Carlotta, Filippini. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza
Fil: Oehm, Andreas W.. Universitat Zurich; Suiza
Fil: Maja, Ruetten. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza
Fil: Andrew, Hemphill. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza
Fil: Frey, Caroline F.. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza
Fil: Basso, Walter Ubaldo. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; Argentina
Materia
SARCOCYSTIS SUIHOMINIS
PCR
SEQUENCING
PHYLOGENY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
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oai:ri.conicet.gov.ar:11336/285248
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in SwitzerlandMoré, Gastón AndrésCarlotta, FilippiniOehm, Andreas W.Maja, RuettenAndrew, HemphillFrey, Caroline F.Basso, Walter UbaldoSARCOCYSTIS SUIHOMINISPCRSEQUENCINGPHYLOGENYhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Sarcocystis spp. and Toxoplasma gondii are heteroxenous protozoan parasites, which form cysts in muscle tissue of intermediate hosts (IH) and oocysts in the intestinal mucosa of definitive hosts (DH). Wild boars (Sus scrofa) are IH for Sarcocystis miescheriana (with wild and domestic canids as DH), S. suihominis (with humans and nonhuman primates as DH) and T. gondii (with felids as DH). The aim of this study was to identify and characterize Sarcocystis spp. and T. gondii in the muscle tissue of wild boars hunted and/or consumed in Switzerland. DNA was extracted from muscle samples of 286 wild boars and tested by PCRs targeting the 18S rRNA gene of Sarcocystis spp., the mitochondrial cytochrome c oxidase (cox1) gene of S. suihominis and by a qPCR targeting a 529 bp genomic repeat of T. gondii. Besides, 225 samples were processed by homogenization and direct microscopic examination and 42 samples by histopathology. Sarcocysts were detected microscopically in 89.3 % (201/225) and 35.7 % (15/42) of the samples, respectively. The 18S rRNA PCR detected parasite DNA in 91.3 % (261/286) of the samples, and all 62 sequences obtained were 100 % identical, and also with GenBank sequences reported as S. miescheriana, suggesting a high environmental contamination with canid faeces. The S. suihominiscox1 PCR was positive in 1.75 % of the samples (5/286), and the six cox1 sequences (772 bp primers trimmed) obtained were 97.3–99.6 % similar among them and 97.7–100 % identical with a sequence reported as S. suihominis. Despite the identity differences, all these sequences were grouped together in a phylogenetic tree, in a sister clade of S. miescheriana sequences. All samples tested negative for T. gondii by qPCR. This study revealed for the first time the presence of S. suihominis in wild boars hunted in Switzerland.Fil: Moré, Gastón Andrés. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Carlotta, Filippini. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; SuizaFil: Oehm, Andreas W.. Universitat Zurich; SuizaFil: Maja, Ruetten. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; SuizaFil: Andrew, Hemphill. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; SuizaFil: Frey, Caroline F.. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; SuizaFil: Basso, Walter Ubaldo. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaElsevier2025-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/285248Moré, Gastón Andrés; Carlotta, Filippini; Oehm, Andreas W.; Maja, Ruetten; Andrew, Hemphill; et al.; Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland; Elsevier; International Journal for Parasitology: Parasites and Wildlife; 27; 8-2025; 1-72213-2244CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2213224425000793info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijppaw.2025.101114info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-17T09:39:13Zoai:ri.conicet.gov.ar:11336/285248instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-17 09:39:13.483CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland
title Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland
spellingShingle Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland
Moré, Gastón Andrés
SARCOCYSTIS SUIHOMINIS
PCR
SEQUENCING
PHYLOGENY
title_short Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland
title_full Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland
title_fullStr Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland
title_full_unstemmed Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland
title_sort Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland
dc.creator.none.fl_str_mv Moré, Gastón Andrés
Carlotta, Filippini
Oehm, Andreas W.
Maja, Ruetten
Andrew, Hemphill
Frey, Caroline F.
Basso, Walter Ubaldo
author Moré, Gastón Andrés
author_facet Moré, Gastón Andrés
Carlotta, Filippini
Oehm, Andreas W.
Maja, Ruetten
Andrew, Hemphill
Frey, Caroline F.
Basso, Walter Ubaldo
author_role author
author2 Carlotta, Filippini
Oehm, Andreas W.
Maja, Ruetten
Andrew, Hemphill
Frey, Caroline F.
Basso, Walter Ubaldo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv SARCOCYSTIS SUIHOMINIS
PCR
SEQUENCING
PHYLOGENY
topic SARCOCYSTIS SUIHOMINIS
PCR
SEQUENCING
PHYLOGENY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Sarcocystis spp. and Toxoplasma gondii are heteroxenous protozoan parasites, which form cysts in muscle tissue of intermediate hosts (IH) and oocysts in the intestinal mucosa of definitive hosts (DH). Wild boars (Sus scrofa) are IH for Sarcocystis miescheriana (with wild and domestic canids as DH), S. suihominis (with humans and nonhuman primates as DH) and T. gondii (with felids as DH). The aim of this study was to identify and characterize Sarcocystis spp. and T. gondii in the muscle tissue of wild boars hunted and/or consumed in Switzerland. DNA was extracted from muscle samples of 286 wild boars and tested by PCRs targeting the 18S rRNA gene of Sarcocystis spp., the mitochondrial cytochrome c oxidase (cox1) gene of S. suihominis and by a qPCR targeting a 529 bp genomic repeat of T. gondii. Besides, 225 samples were processed by homogenization and direct microscopic examination and 42 samples by histopathology. Sarcocysts were detected microscopically in 89.3 % (201/225) and 35.7 % (15/42) of the samples, respectively. The 18S rRNA PCR detected parasite DNA in 91.3 % (261/286) of the samples, and all 62 sequences obtained were 100 % identical, and also with GenBank sequences reported as S. miescheriana, suggesting a high environmental contamination with canid faeces. The S. suihominiscox1 PCR was positive in 1.75 % of the samples (5/286), and the six cox1 sequences (772 bp primers trimmed) obtained were 97.3–99.6 % similar among them and 97.7–100 % identical with a sequence reported as S. suihominis. Despite the identity differences, all these sequences were grouped together in a phylogenetic tree, in a sister clade of S. miescheriana sequences. All samples tested negative for T. gondii by qPCR. This study revealed for the first time the presence of S. suihominis in wild boars hunted in Switzerland.
Fil: Moré, Gastón Andrés. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Carlotta, Filippini. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza
Fil: Oehm, Andreas W.. Universitat Zurich; Suiza
Fil: Maja, Ruetten. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza
Fil: Andrew, Hemphill. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza
Fil: Frey, Caroline F.. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza
Fil: Basso, Walter Ubaldo. University Of Berne. Faculties Of Veterinary And Medicine. Institute Of Parasitology; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; Argentina
description Sarcocystis spp. and Toxoplasma gondii are heteroxenous protozoan parasites, which form cysts in muscle tissue of intermediate hosts (IH) and oocysts in the intestinal mucosa of definitive hosts (DH). Wild boars (Sus scrofa) are IH for Sarcocystis miescheriana (with wild and domestic canids as DH), S. suihominis (with humans and nonhuman primates as DH) and T. gondii (with felids as DH). The aim of this study was to identify and characterize Sarcocystis spp. and T. gondii in the muscle tissue of wild boars hunted and/or consumed in Switzerland. DNA was extracted from muscle samples of 286 wild boars and tested by PCRs targeting the 18S rRNA gene of Sarcocystis spp., the mitochondrial cytochrome c oxidase (cox1) gene of S. suihominis and by a qPCR targeting a 529 bp genomic repeat of T. gondii. Besides, 225 samples were processed by homogenization and direct microscopic examination and 42 samples by histopathology. Sarcocysts were detected microscopically in 89.3 % (201/225) and 35.7 % (15/42) of the samples, respectively. The 18S rRNA PCR detected parasite DNA in 91.3 % (261/286) of the samples, and all 62 sequences obtained were 100 % identical, and also with GenBank sequences reported as S. miescheriana, suggesting a high environmental contamination with canid faeces. The S. suihominiscox1 PCR was positive in 1.75 % of the samples (5/286), and the six cox1 sequences (772 bp primers trimmed) obtained were 97.3–99.6 % similar among them and 97.7–100 % identical with a sequence reported as S. suihominis. Despite the identity differences, all these sequences were grouped together in a phylogenetic tree, in a sister clade of S. miescheriana sequences. All samples tested negative for T. gondii by qPCR. This study revealed for the first time the presence of S. suihominis in wild boars hunted in Switzerland.
publishDate 2025
dc.date.none.fl_str_mv 2025-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/285248
Moré, Gastón Andrés; Carlotta, Filippini; Oehm, Andreas W.; Maja, Ruetten; Andrew, Hemphill; et al.; Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland; Elsevier; International Journal for Parasitology: Parasites and Wildlife; 27; 8-2025; 1-7
2213-2244
CONICET Digital
CONICET
url http://hdl.handle.net/11336/285248
identifier_str_mv Moré, Gastón Andrés; Carlotta, Filippini; Oehm, Andreas W.; Maja, Ruetten; Andrew, Hemphill; et al.; Sarcocystis spp. and Toxoplasma gondii in muscles from wild boars (Sus scrofa) consumed in Switzerland; Elsevier; International Journal for Parasitology: Parasites and Wildlife; 27; 8-2025; 1-7
2213-2244
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2213224425000793
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijppaw.2025.101114
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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