Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina

Autores
Helman, Elisa; Dellarupe, Andrea; Cifuentes, Sabrina; Chang Reissig, Elizabeth; Moré, Gastón
Año de publicación
2022
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sarcocystis spp. are intracellular protozoan parasites with an obligatory heteroxenous life cycle. The objective of this study is to identify Sarcocystis spp. in wild boar muscles from Argentina by light and transmission electron microscopy and molecular characterization. Muscle samples from diaphragm, tongue, masseter, intercostals, heart, and forelimbs of 240 wild boars were analyzed. Of the animals, 48.3% (116/240) were positive for sarcocysts by light microscopy, whereas 45.8% (110/240) were positive for Sarcocystis spp. by PCR targeting 18S rRNA fragment. These samples were subjected to a specific PCR for S. suihominis coxI gene, 3.6% (4/110) of which were weak positives. Unfortunately, sequence analysis was inconclusive. This could be related to a potentially low S. suihominis cyst load in the samples, or to an incomplete primer matching with the South American S. suihominis sequences. Seventeen individual sarcocysts were positive by PCR for the 18S rRNA fragment, whose sequences showed 99.75–100% identity with each other and with previously reported S.miescheriana sequences. A total of 21 cysts collected from 11 muscle samples and analyzed by TEM presented a cyst wall type compatible with S. miescheriana, and one cyst presented an ultrastructure compatible with S. suihominis. The latter came from a sample that also contained S. miescheriana cysts, indicating that the animal was co-infected. This is the first study that provides infection rates and describes and identifies morphological and molecular features of Sarcocystis spp. cysts in wild boars from South America.
Estación Experimental Agropecuaria Bariloche
Fil: Helman, Elisa. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina
Fil: Helman, Elisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Dellarupe, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina
Fil: Dellarupe, Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Cifuentes, Sabrina. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Chang Reissig, Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Chang Reissig, Elizabeth. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina
Fil: Moré, Gastón. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fuente
Parasitology Research (Published: 06 December 2022)
Materia
Sarcocystis
Sarcocystis miescheriana
Parásitos
Enfermedades Parasitarias
Parasites
Parasitoses
Wild Boar
Sus scrofa
Jabalí
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/13787
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network_name_str INTA Digital (INTA)
spelling Identification of Sarcocystis spp. in wild boars (Sus scrofa) from ArgentinaHelman, ElisaDellarupe, AndreaCifuentes, SabrinaChang Reissig, ElizabethMoré, GastónSarcocystisSarcocystis miescherianaParásitosEnfermedades ParasitariasParasitesParasitosesWild BoarSus scrofaJabalíArgentinaSarcocystis spp. are intracellular protozoan parasites with an obligatory heteroxenous life cycle. The objective of this study is to identify Sarcocystis spp. in wild boar muscles from Argentina by light and transmission electron microscopy and molecular characterization. Muscle samples from diaphragm, tongue, masseter, intercostals, heart, and forelimbs of 240 wild boars were analyzed. Of the animals, 48.3% (116/240) were positive for sarcocysts by light microscopy, whereas 45.8% (110/240) were positive for Sarcocystis spp. by PCR targeting 18S rRNA fragment. These samples were subjected to a specific PCR for S. suihominis coxI gene, 3.6% (4/110) of which were weak positives. Unfortunately, sequence analysis was inconclusive. This could be related to a potentially low S. suihominis cyst load in the samples, or to an incomplete primer matching with the South American S. suihominis sequences. Seventeen individual sarcocysts were positive by PCR for the 18S rRNA fragment, whose sequences showed 99.75–100% identity with each other and with previously reported S.miescheriana sequences. A total of 21 cysts collected from 11 muscle samples and analyzed by TEM presented a cyst wall type compatible with S. miescheriana, and one cyst presented an ultrastructure compatible with S. suihominis. The latter came from a sample that also contained S. miescheriana cysts, indicating that the animal was co-infected. This is the first study that provides infection rates and describes and identifies morphological and molecular features of Sarcocystis spp. cysts in wild boars from South America.Estación Experimental Agropecuaria BarilocheFil: Helman, Elisa. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; ArgentinaFil: Helman, Elisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas; ArgentinaFil: Dellarupe, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; ArgentinaFil: Dellarupe, Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas; ArgentinaFil: Cifuentes, Sabrina. Consejo Nacional de Investigaciones Cientificas y Tecnicas; ArgentinaFil: Chang Reissig, Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: Chang Reissig, Elizabeth. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; ArgentinaFil: Moré, Gastón. Consejo Nacional de Investigaciones Cientificas y Tecnicas; ArgentinaSpringer2023-01-03T09:54:24Z2023-01-03T09:54:24Z2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13787https://link.springer.com/article/10.1007/s00436-022-07743-w0932-01131432-1955https://doi.org/10.1007/s00436-022-07743-wParasitology Research (Published: 06 December 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaspainfo:eu-repo/semantics/restrictedAccess2025-09-18T10:08:52Zoai:localhost:20.500.12123/13787instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:08:52.443INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina
title Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina
spellingShingle Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina
Helman, Elisa
Sarcocystis
Sarcocystis miescheriana
Parásitos
Enfermedades Parasitarias
Parasites
Parasitoses
Wild Boar
Sus scrofa
Jabalí
Argentina
title_short Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina
title_full Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina
title_fullStr Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina
title_full_unstemmed Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina
title_sort Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina
dc.creator.none.fl_str_mv Helman, Elisa
Dellarupe, Andrea
Cifuentes, Sabrina
Chang Reissig, Elizabeth
Moré, Gastón
author Helman, Elisa
author_facet Helman, Elisa
Dellarupe, Andrea
Cifuentes, Sabrina
Chang Reissig, Elizabeth
Moré, Gastón
author_role author
author2 Dellarupe, Andrea
Cifuentes, Sabrina
Chang Reissig, Elizabeth
Moré, Gastón
author2_role author
author
author
author
dc.subject.none.fl_str_mv Sarcocystis
Sarcocystis miescheriana
Parásitos
Enfermedades Parasitarias
Parasites
Parasitoses
Wild Boar
Sus scrofa
Jabalí
Argentina
topic Sarcocystis
Sarcocystis miescheriana
Parásitos
Enfermedades Parasitarias
Parasites
Parasitoses
Wild Boar
Sus scrofa
Jabalí
Argentina
dc.description.none.fl_txt_mv Sarcocystis spp. are intracellular protozoan parasites with an obligatory heteroxenous life cycle. The objective of this study is to identify Sarcocystis spp. in wild boar muscles from Argentina by light and transmission electron microscopy and molecular characterization. Muscle samples from diaphragm, tongue, masseter, intercostals, heart, and forelimbs of 240 wild boars were analyzed. Of the animals, 48.3% (116/240) were positive for sarcocysts by light microscopy, whereas 45.8% (110/240) were positive for Sarcocystis spp. by PCR targeting 18S rRNA fragment. These samples were subjected to a specific PCR for S. suihominis coxI gene, 3.6% (4/110) of which were weak positives. Unfortunately, sequence analysis was inconclusive. This could be related to a potentially low S. suihominis cyst load in the samples, or to an incomplete primer matching with the South American S. suihominis sequences. Seventeen individual sarcocysts were positive by PCR for the 18S rRNA fragment, whose sequences showed 99.75–100% identity with each other and with previously reported S.miescheriana sequences. A total of 21 cysts collected from 11 muscle samples and analyzed by TEM presented a cyst wall type compatible with S. miescheriana, and one cyst presented an ultrastructure compatible with S. suihominis. The latter came from a sample that also contained S. miescheriana cysts, indicating that the animal was co-infected. This is the first study that provides infection rates and describes and identifies morphological and molecular features of Sarcocystis spp. cysts in wild boars from South America.
Estación Experimental Agropecuaria Bariloche
Fil: Helman, Elisa. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina
Fil: Helman, Elisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Dellarupe, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina
Fil: Dellarupe, Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Cifuentes, Sabrina. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Chang Reissig, Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Chang Reissig, Elizabeth. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina
Fil: Moré, Gastón. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
description Sarcocystis spp. are intracellular protozoan parasites with an obligatory heteroxenous life cycle. The objective of this study is to identify Sarcocystis spp. in wild boar muscles from Argentina by light and transmission electron microscopy and molecular characterization. Muscle samples from diaphragm, tongue, masseter, intercostals, heart, and forelimbs of 240 wild boars were analyzed. Of the animals, 48.3% (116/240) were positive for sarcocysts by light microscopy, whereas 45.8% (110/240) were positive for Sarcocystis spp. by PCR targeting 18S rRNA fragment. These samples were subjected to a specific PCR for S. suihominis coxI gene, 3.6% (4/110) of which were weak positives. Unfortunately, sequence analysis was inconclusive. This could be related to a potentially low S. suihominis cyst load in the samples, or to an incomplete primer matching with the South American S. suihominis sequences. Seventeen individual sarcocysts were positive by PCR for the 18S rRNA fragment, whose sequences showed 99.75–100% identity with each other and with previously reported S.miescheriana sequences. A total of 21 cysts collected from 11 muscle samples and analyzed by TEM presented a cyst wall type compatible with S. miescheriana, and one cyst presented an ultrastructure compatible with S. suihominis. The latter came from a sample that also contained S. miescheriana cysts, indicating that the animal was co-infected. This is the first study that provides infection rates and describes and identifies morphological and molecular features of Sarcocystis spp. cysts in wild boars from South America.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
2023-01-03T09:54:24Z
2023-01-03T09:54:24Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13787
https://link.springer.com/article/10.1007/s00436-022-07743-w
0932-0113
1432-1955
https://doi.org/10.1007/s00436-022-07743-w
url http://hdl.handle.net/20.500.12123/13787
https://link.springer.com/article/10.1007/s00436-022-07743-w
https://doi.org/10.1007/s00436-022-07743-w
identifier_str_mv 0932-0113
1432-1955
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Parasitology Research (Published: 06 December 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.001348

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