Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany

Autores
Moré, Gastón Andrés; Maksimov, A.; Conraths, F. J.; Schares, G.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
More than 200 Sarcocystis spp. have been named and most of them appear to be involved in a particular predator-prey cycle. Among canids, the European fox (Vulpes vulpes) and the raccoon dog (Nyctereutes procyonoides) are widely distributed in Europe and probably play an important role as definitive hosts in the epidemiology of Sarcocystis spp. infections. A total of 50 small intestines from foxes and 38 from raccoon dogs were sampled in the Federal State of Brandenburg, Germany. Mucosal scrapings were collected and analyzed by sugar flotation and when oocysts or sporocysts were detected, an overnight sedimentation was performed and DNA extracted with a commercial kit. A PCR was conducted using primers targeting a fragment of the 18S rRNA gene (with a size of approximately 850 bp) and the amplicons were purified and sequenced. Samples with an inconclusive sequencing were cloned into plasmids and ≥ 3 plasmids from each amplicon were sequenced. Sarcocystis spp. oocysts/sporocysts were detected in 38% (19/50) of fox and 52.6% (20/38) of raccoon dog samples. Sequencing analysis of amplicons from oocyst DNA revealed mixed infections in 9 fox and 5 raccoon dog samples. In the fox samples, the most often identified Sarcocystis spp. were S. tenella or S. capracanis (10.0%); S. miescheriana (8.0%) and S. gracilis (8.0%) followed by Sarcocystis spp., which use birds as intermediate hosts (6.0%), and S. capreolicanis (4.0%). In the raccoon dog samples, sequences with a ≥99% identity with the following species were detected: S. miescheriana (18.4%), S. gracilis (13.1%), Sarcocystis spp. using birds as IH (10.5%), S. tenella or S.capracanis (2.6%) and S. capreolicanis (2.6%). The estimated prevalence of Sarcocystis spp. infections determined using mucosal scrapings was higher than in related studies performed by analyzing faecal samples. The methodology of 18S rRNA gene amplification, cloning and sequencing is suitable to identify mixed infections with Sarcocystis spp. and to gather information on potential definitive hosts of these parasite species.
Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania
Fil: Maksimov, A.. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania
Fil: Conraths, F. J.. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania
Fil: Schares, G.. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania
Materia
Definitive Host
Mucosal Scraping
Pcr
Sarcocystis Spp.
Sequencing
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54072

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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from GermanyMoré, Gastón AndrésMaksimov, A.Conraths, F. J.Schares, G.Definitive HostMucosal ScrapingPcrSarcocystis Spp.Sequencinghttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4More than 200 Sarcocystis spp. have been named and most of them appear to be involved in a particular predator-prey cycle. Among canids, the European fox (Vulpes vulpes) and the raccoon dog (Nyctereutes procyonoides) are widely distributed in Europe and probably play an important role as definitive hosts in the epidemiology of Sarcocystis spp. infections. A total of 50 small intestines from foxes and 38 from raccoon dogs were sampled in the Federal State of Brandenburg, Germany. Mucosal scrapings were collected and analyzed by sugar flotation and when oocysts or sporocysts were detected, an overnight sedimentation was performed and DNA extracted with a commercial kit. A PCR was conducted using primers targeting a fragment of the 18S rRNA gene (with a size of approximately 850 bp) and the amplicons were purified and sequenced. Samples with an inconclusive sequencing were cloned into plasmids and ≥ 3 plasmids from each amplicon were sequenced. Sarcocystis spp. oocysts/sporocysts were detected in 38% (19/50) of fox and 52.6% (20/38) of raccoon dog samples. Sequencing analysis of amplicons from oocyst DNA revealed mixed infections in 9 fox and 5 raccoon dog samples. In the fox samples, the most often identified Sarcocystis spp. were S. tenella or S. capracanis (10.0%); S. miescheriana (8.0%) and S. gracilis (8.0%) followed by Sarcocystis spp., which use birds as intermediate hosts (6.0%), and S. capreolicanis (4.0%). In the raccoon dog samples, sequences with a ≥99% identity with the following species were detected: S. miescheriana (18.4%), S. gracilis (13.1%), Sarcocystis spp. using birds as IH (10.5%), S. tenella or S.capracanis (2.6%) and S. capreolicanis (2.6%). The estimated prevalence of Sarcocystis spp. infections determined using mucosal scrapings was higher than in related studies performed by analyzing faecal samples. The methodology of 18S rRNA gene amplification, cloning and sequencing is suitable to identify mixed infections with Sarcocystis spp. and to gather information on potential definitive hosts of these parasite species.Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; AlemaniaFil: Maksimov, A.. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; AlemaniaFil: Conraths, F. J.. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; AlemaniaFil: Schares, G.. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; AlemaniaElsevier Science2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54072Moré, Gastón Andrés; Maksimov, A.; Conraths, F. J.; Schares, G.; Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany; Elsevier Science; Veterinary Parasitology; 220; 4-2016; 9-140304-4017CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304401716300292info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2016.02.011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:49Zoai:ri.conicet.gov.ar:11336/54072instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:49.713CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany
title Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany
spellingShingle Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany
Moré, Gastón Andrés
Definitive Host
Mucosal Scraping
Pcr
Sarcocystis Spp.
Sequencing
title_short Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany
title_full Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany
title_fullStr Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany
title_full_unstemmed Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany
title_sort Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany
dc.creator.none.fl_str_mv Moré, Gastón Andrés
Maksimov, A.
Conraths, F. J.
Schares, G.
author Moré, Gastón Andrés
author_facet Moré, Gastón Andrés
Maksimov, A.
Conraths, F. J.
Schares, G.
author_role author
author2 Maksimov, A.
Conraths, F. J.
Schares, G.
author2_role author
author
author
dc.subject.none.fl_str_mv Definitive Host
Mucosal Scraping
Pcr
Sarcocystis Spp.
Sequencing
topic Definitive Host
Mucosal Scraping
Pcr
Sarcocystis Spp.
Sequencing
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv More than 200 Sarcocystis spp. have been named and most of them appear to be involved in a particular predator-prey cycle. Among canids, the European fox (Vulpes vulpes) and the raccoon dog (Nyctereutes procyonoides) are widely distributed in Europe and probably play an important role as definitive hosts in the epidemiology of Sarcocystis spp. infections. A total of 50 small intestines from foxes and 38 from raccoon dogs were sampled in the Federal State of Brandenburg, Germany. Mucosal scrapings were collected and analyzed by sugar flotation and when oocysts or sporocysts were detected, an overnight sedimentation was performed and DNA extracted with a commercial kit. A PCR was conducted using primers targeting a fragment of the 18S rRNA gene (with a size of approximately 850 bp) and the amplicons were purified and sequenced. Samples with an inconclusive sequencing were cloned into plasmids and ≥ 3 plasmids from each amplicon were sequenced. Sarcocystis spp. oocysts/sporocysts were detected in 38% (19/50) of fox and 52.6% (20/38) of raccoon dog samples. Sequencing analysis of amplicons from oocyst DNA revealed mixed infections in 9 fox and 5 raccoon dog samples. In the fox samples, the most often identified Sarcocystis spp. were S. tenella or S. capracanis (10.0%); S. miescheriana (8.0%) and S. gracilis (8.0%) followed by Sarcocystis spp., which use birds as intermediate hosts (6.0%), and S. capreolicanis (4.0%). In the raccoon dog samples, sequences with a ≥99% identity with the following species were detected: S. miescheriana (18.4%), S. gracilis (13.1%), Sarcocystis spp. using birds as IH (10.5%), S. tenella or S.capracanis (2.6%) and S. capreolicanis (2.6%). The estimated prevalence of Sarcocystis spp. infections determined using mucosal scrapings was higher than in related studies performed by analyzing faecal samples. The methodology of 18S rRNA gene amplification, cloning and sequencing is suitable to identify mixed infections with Sarcocystis spp. and to gather information on potential definitive hosts of these parasite species.
Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania
Fil: Maksimov, A.. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania
Fil: Conraths, F. J.. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania
Fil: Schares, G.. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania
description More than 200 Sarcocystis spp. have been named and most of them appear to be involved in a particular predator-prey cycle. Among canids, the European fox (Vulpes vulpes) and the raccoon dog (Nyctereutes procyonoides) are widely distributed in Europe and probably play an important role as definitive hosts in the epidemiology of Sarcocystis spp. infections. A total of 50 small intestines from foxes and 38 from raccoon dogs were sampled in the Federal State of Brandenburg, Germany. Mucosal scrapings were collected and analyzed by sugar flotation and when oocysts or sporocysts were detected, an overnight sedimentation was performed and DNA extracted with a commercial kit. A PCR was conducted using primers targeting a fragment of the 18S rRNA gene (with a size of approximately 850 bp) and the amplicons were purified and sequenced. Samples with an inconclusive sequencing were cloned into plasmids and ≥ 3 plasmids from each amplicon were sequenced. Sarcocystis spp. oocysts/sporocysts were detected in 38% (19/50) of fox and 52.6% (20/38) of raccoon dog samples. Sequencing analysis of amplicons from oocyst DNA revealed mixed infections in 9 fox and 5 raccoon dog samples. In the fox samples, the most often identified Sarcocystis spp. were S. tenella or S. capracanis (10.0%); S. miescheriana (8.0%) and S. gracilis (8.0%) followed by Sarcocystis spp., which use birds as intermediate hosts (6.0%), and S. capreolicanis (4.0%). In the raccoon dog samples, sequences with a ≥99% identity with the following species were detected: S. miescheriana (18.4%), S. gracilis (13.1%), Sarcocystis spp. using birds as IH (10.5%), S. tenella or S.capracanis (2.6%) and S. capreolicanis (2.6%). The estimated prevalence of Sarcocystis spp. infections determined using mucosal scrapings was higher than in related studies performed by analyzing faecal samples. The methodology of 18S rRNA gene amplification, cloning and sequencing is suitable to identify mixed infections with Sarcocystis spp. and to gather information on potential definitive hosts of these parasite species.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54072
Moré, Gastón Andrés; Maksimov, A.; Conraths, F. J.; Schares, G.; Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany; Elsevier Science; Veterinary Parasitology; 220; 4-2016; 9-14
0304-4017
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54072
identifier_str_mv Moré, Gastón Andrés; Maksimov, A.; Conraths, F. J.; Schares, G.; Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany; Elsevier Science; Veterinary Parasitology; 220; 4-2016; 9-14
0304-4017
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304401716300292
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2016.02.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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