Prevalence of Sarcocystis spp. in Argentinean cattle

Autores
Moré, Gastón Andrés; Abrahamovich, P.; Jurado, Susana Beatriz; Bacigalupe, Diana; Marin, J.C.; Rambeaud, Magdalena; Venturini, L.; Venturini, M.C.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sarcocystis cruzi, S. hirsuta and S. hominis are apicomplexan parasites that affect cattle worldwide with variable prevalence. The aim of the present study was to evaluate the prevalence of Sarcocystis spp. in Argentinean cattle comparing microscopic fresh examination and molecular methods. Blood, myocardium and loin samples were collected in five slaughterhouses from a total of 380 bovines. Origin of animals was representative of the major beef cattle production area of Argentina. Samples were analyzed by fresh microscopical examination, transmission electron microscopy (TEM), IFAT and PCR-RFLP. Thin walled sarcocysts corresponding with S. cruzi were found in 99.5% of heart samples. Sarcocysts were detected in 73.1% of loin samples; 71.5% had S. cruzi cysts and 23.1% had thick walled sarcocysts (S. hirsuta or S. hominis). TEM observation revealed the presence of characteristic S. hominis and S. hirsuta cyst walls in 7 and 1 loin samples respectively. Using IFAT, 379/380 animals had titers 25 or higher, showing a full agreement with fresh examination. Amplification products were detected in 35.5% (135/380) of loin samples; however Sarcocystis species could only be determined by RFLP in 29 samples. Agreement between fresh examination and PCR was low (Kappa value = 0.262). This is the first report of S. hominis and S. hirsuta in Argentina. Further studies are needed to improve the sensitivity of molecular methods for species identification, especially for differentiation of S. cruzi and S. hirsuta from the zoonotic species S. hominis. The results of the present study and others focusing on sensitivity and specificity of Sarcocystis spp. diagnostic methods should contribute to improve food safety.
Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Abrahamovich, P.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Jurado, Susana Beatriz. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Bacigalupe, Diana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Marin, J.C.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Rambeaud, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Venturini, L.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Venturini, M.C.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Materia
DIAGNOSIS
FOOD SAFETY
FRESH EXAMINATION
PCR-RFLP
SARCOCYSTIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95063

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network_name_str CONICET Digital (CONICET)
spelling Prevalence of Sarcocystis spp. in Argentinean cattleMoré, Gastón AndrésAbrahamovich, P.Jurado, Susana BeatrizBacigalupe, DianaMarin, J.C.Rambeaud, MagdalenaVenturini, L.Venturini, M.C.DIAGNOSISFOOD SAFETYFRESH EXAMINATIONPCR-RFLPSARCOCYSTIShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Sarcocystis cruzi, S. hirsuta and S. hominis are apicomplexan parasites that affect cattle worldwide with variable prevalence. The aim of the present study was to evaluate the prevalence of Sarcocystis spp. in Argentinean cattle comparing microscopic fresh examination and molecular methods. Blood, myocardium and loin samples were collected in five slaughterhouses from a total of 380 bovines. Origin of animals was representative of the major beef cattle production area of Argentina. Samples were analyzed by fresh microscopical examination, transmission electron microscopy (TEM), IFAT and PCR-RFLP. Thin walled sarcocysts corresponding with S. cruzi were found in 99.5% of heart samples. Sarcocysts were detected in 73.1% of loin samples; 71.5% had S. cruzi cysts and 23.1% had thick walled sarcocysts (S. hirsuta or S. hominis). TEM observation revealed the presence of characteristic S. hominis and S. hirsuta cyst walls in 7 and 1 loin samples respectively. Using IFAT, 379/380 animals had titers 25 or higher, showing a full agreement with fresh examination. Amplification products were detected in 35.5% (135/380) of loin samples; however Sarcocystis species could only be determined by RFLP in 29 samples. Agreement between fresh examination and PCR was low (Kappa value = 0.262). This is the first report of S. hominis and S. hirsuta in Argentina. Further studies are needed to improve the sensitivity of molecular methods for species identification, especially for differentiation of S. cruzi and S. hirsuta from the zoonotic species S. hominis. The results of the present study and others focusing on sensitivity and specificity of Sarcocystis spp. diagnostic methods should contribute to improve food safety.Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; ArgentinaFil: Abrahamovich, P.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; ArgentinaFil: Jurado, Susana Beatriz. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Bacigalupe, Diana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; ArgentinaFil: Marin, J.C.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; ArgentinaFil: Rambeaud, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; ArgentinaFil: Venturini, L.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; ArgentinaFil: Venturini, M.C.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; ArgentinaElsevier Science2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95063Moré, Gastón Andrés; Abrahamovich, P.; Jurado, Susana Beatriz; Bacigalupe, Diana; Marin, J.C.; et al.; Prevalence of Sarcocystis spp. in Argentinean cattle; Elsevier Science; Veterinary Parasitology; 177; 1-2; 4-2011; 162-1650304-4017CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304401710006862info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2010.11.036info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:09Zoai:ri.conicet.gov.ar:11336/95063instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:09.473CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prevalence of Sarcocystis spp. in Argentinean cattle
title Prevalence of Sarcocystis spp. in Argentinean cattle
spellingShingle Prevalence of Sarcocystis spp. in Argentinean cattle
Moré, Gastón Andrés
DIAGNOSIS
FOOD SAFETY
FRESH EXAMINATION
PCR-RFLP
SARCOCYSTIS
title_short Prevalence of Sarcocystis spp. in Argentinean cattle
title_full Prevalence of Sarcocystis spp. in Argentinean cattle
title_fullStr Prevalence of Sarcocystis spp. in Argentinean cattle
title_full_unstemmed Prevalence of Sarcocystis spp. in Argentinean cattle
title_sort Prevalence of Sarcocystis spp. in Argentinean cattle
dc.creator.none.fl_str_mv Moré, Gastón Andrés
Abrahamovich, P.
Jurado, Susana Beatriz
Bacigalupe, Diana
Marin, J.C.
Rambeaud, Magdalena
Venturini, L.
Venturini, M.C.
author Moré, Gastón Andrés
author_facet Moré, Gastón Andrés
Abrahamovich, P.
Jurado, Susana Beatriz
Bacigalupe, Diana
Marin, J.C.
Rambeaud, Magdalena
Venturini, L.
Venturini, M.C.
author_role author
author2 Abrahamovich, P.
Jurado, Susana Beatriz
Bacigalupe, Diana
Marin, J.C.
Rambeaud, Magdalena
Venturini, L.
Venturini, M.C.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DIAGNOSIS
FOOD SAFETY
FRESH EXAMINATION
PCR-RFLP
SARCOCYSTIS
topic DIAGNOSIS
FOOD SAFETY
FRESH EXAMINATION
PCR-RFLP
SARCOCYSTIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Sarcocystis cruzi, S. hirsuta and S. hominis are apicomplexan parasites that affect cattle worldwide with variable prevalence. The aim of the present study was to evaluate the prevalence of Sarcocystis spp. in Argentinean cattle comparing microscopic fresh examination and molecular methods. Blood, myocardium and loin samples were collected in five slaughterhouses from a total of 380 bovines. Origin of animals was representative of the major beef cattle production area of Argentina. Samples were analyzed by fresh microscopical examination, transmission electron microscopy (TEM), IFAT and PCR-RFLP. Thin walled sarcocysts corresponding with S. cruzi were found in 99.5% of heart samples. Sarcocysts were detected in 73.1% of loin samples; 71.5% had S. cruzi cysts and 23.1% had thick walled sarcocysts (S. hirsuta or S. hominis). TEM observation revealed the presence of characteristic S. hominis and S. hirsuta cyst walls in 7 and 1 loin samples respectively. Using IFAT, 379/380 animals had titers 25 or higher, showing a full agreement with fresh examination. Amplification products were detected in 35.5% (135/380) of loin samples; however Sarcocystis species could only be determined by RFLP in 29 samples. Agreement between fresh examination and PCR was low (Kappa value = 0.262). This is the first report of S. hominis and S. hirsuta in Argentina. Further studies are needed to improve the sensitivity of molecular methods for species identification, especially for differentiation of S. cruzi and S. hirsuta from the zoonotic species S. hominis. The results of the present study and others focusing on sensitivity and specificity of Sarcocystis spp. diagnostic methods should contribute to improve food safety.
Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Abrahamovich, P.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Jurado, Susana Beatriz. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Bacigalupe, Diana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Marin, J.C.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Rambeaud, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Venturini, L.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Fil: Venturini, M.C.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
description Sarcocystis cruzi, S. hirsuta and S. hominis are apicomplexan parasites that affect cattle worldwide with variable prevalence. The aim of the present study was to evaluate the prevalence of Sarcocystis spp. in Argentinean cattle comparing microscopic fresh examination and molecular methods. Blood, myocardium and loin samples were collected in five slaughterhouses from a total of 380 bovines. Origin of animals was representative of the major beef cattle production area of Argentina. Samples were analyzed by fresh microscopical examination, transmission electron microscopy (TEM), IFAT and PCR-RFLP. Thin walled sarcocysts corresponding with S. cruzi were found in 99.5% of heart samples. Sarcocysts were detected in 73.1% of loin samples; 71.5% had S. cruzi cysts and 23.1% had thick walled sarcocysts (S. hirsuta or S. hominis). TEM observation revealed the presence of characteristic S. hominis and S. hirsuta cyst walls in 7 and 1 loin samples respectively. Using IFAT, 379/380 animals had titers 25 or higher, showing a full agreement with fresh examination. Amplification products were detected in 35.5% (135/380) of loin samples; however Sarcocystis species could only be determined by RFLP in 29 samples. Agreement between fresh examination and PCR was low (Kappa value = 0.262). This is the first report of S. hominis and S. hirsuta in Argentina. Further studies are needed to improve the sensitivity of molecular methods for species identification, especially for differentiation of S. cruzi and S. hirsuta from the zoonotic species S. hominis. The results of the present study and others focusing on sensitivity and specificity of Sarcocystis spp. diagnostic methods should contribute to improve food safety.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95063
Moré, Gastón Andrés; Abrahamovich, P.; Jurado, Susana Beatriz; Bacigalupe, Diana; Marin, J.C.; et al.; Prevalence of Sarcocystis spp. in Argentinean cattle; Elsevier Science; Veterinary Parasitology; 177; 1-2; 4-2011; 162-165
0304-4017
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95063
identifier_str_mv Moré, Gastón Andrés; Abrahamovich, P.; Jurado, Susana Beatriz; Bacigalupe, Diana; Marin, J.C.; et al.; Prevalence of Sarcocystis spp. in Argentinean cattle; Elsevier Science; Veterinary Parasitology; 177; 1-2; 4-2011; 162-165
0304-4017
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2010.11.036
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier Science
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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