A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus

Autores
Garcia Guizzo, Melina; Parizi, Luís Fernando; Nunes, Rodrigo Dutra; Schama, Renata; Albano, Rodolpho M.; Tirloni, Lucas; Oldiges, Daiane Patrícia; Pilz Vieira, Ricardo; Cruz Oliveira, Wanderson Henrique; De Souza Leite, Milane; Gonzales, Sergio A.; Farber, Marisa Diana; Martins, Orlando; Da Silva Vaz, Itabajara; Oliveira, Pedro L.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts.
Fil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro; Brasil
Fil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Nunes, Rodrigo Dutra. Universidade Federal do Rio de Janeiro; Brasil
Fil: Schama, Renata. Laboratório de Biologia Computacional e Sistemas; Brasil
Fil: Albano, Rodolpho M.. Universidade do Estado do Rio de Janeiro; Brasil
Fil: Tirloni, Lucas. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Oldiges, Daiane Patrícia. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Pilz Vieira, Ricardo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Cruz Oliveira, Wanderson Henrique. Universidade Federal Rural do Rio de Janeiro; Brasil
Fil: De Souza Leite, Milane. Universidade Federal Rural do Rio de Janeiro; Brasil
Fil: Gonzales, Sergio A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Martins, Orlando. Universidade Federal do Rio de Janeiro; Brasil
Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
Materia
APPLIED MICROBIOLOGY
SYMBIOSIS
METAGENOMICS
TICKS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41664
Acceder
id CONICETDig_1e2ef46485efe99309516248b707e903
oai_identifier_str oai:ri.conicet.gov.ar:11336/41664
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplusGarcia Guizzo, MelinaParizi, Luís FernandoNunes, Rodrigo DutraSchama, RenataAlbano, Rodolpho M.Tirloni, LucasOldiges, Daiane PatríciaPilz Vieira, RicardoCruz Oliveira, Wanderson HenriqueDe Souza Leite, MilaneGonzales, Sergio A.Farber, Marisa DianaMartins, OrlandoDa Silva Vaz, ItabajaraOliveira, Pedro L.APPLIED MICROBIOLOGYSYMBIOSISMETAGENOMICSTICKShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts.Fil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro; BrasilFil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul; BrasilFil: Nunes, Rodrigo Dutra. Universidade Federal do Rio de Janeiro; BrasilFil: Schama, Renata. Laboratório de Biologia Computacional e Sistemas; BrasilFil: Albano, Rodolpho M.. Universidade do Estado do Rio de Janeiro; BrasilFil: Tirloni, Lucas. Universidade Federal do Rio Grande do Sul; BrasilFil: Oldiges, Daiane Patrícia. Universidade Federal do Rio Grande do Sul; BrasilFil: Pilz Vieira, Ricardo. Universidade Federal do Rio de Janeiro; BrasilFil: Cruz Oliveira, Wanderson Henrique. Universidade Federal Rural do Rio de Janeiro; BrasilFil: De Souza Leite, Milane. Universidade Federal Rural do Rio de Janeiro; BrasilFil: Gonzales, Sergio A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Martins, Orlando. Universidade Federal do Rio de Janeiro; BrasilFil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; BrasilFil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; BrasilNature Publishing Group2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41664Garcia Guizzo, Melina; Parizi, Luís Fernando; Nunes, Rodrigo Dutra; Schama, Renata; Albano, Rodolpho M.; et al.; A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus; Nature Publishing Group; Scientific Reports; 7; 1; 12-20172045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-17309-xinfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-17309-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:06Zoai:ri.conicet.gov.ar:11336/41664instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:06.869CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus
title A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus
spellingShingle A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus
Garcia Guizzo, Melina
APPLIED MICROBIOLOGY
SYMBIOSIS
METAGENOMICS
TICKS
title_short A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus
title_full A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus
title_fullStr A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus
title_full_unstemmed A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus
title_sort A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus
dc.creator.none.fl_str_mv Garcia Guizzo, Melina
Parizi, Luís Fernando
Nunes, Rodrigo Dutra
Schama, Renata
Albano, Rodolpho M.
Tirloni, Lucas
Oldiges, Daiane Patrícia
Pilz Vieira, Ricardo
Cruz Oliveira, Wanderson Henrique
De Souza Leite, Milane
Gonzales, Sergio A.
Farber, Marisa Diana
Martins, Orlando
Da Silva Vaz, Itabajara
Oliveira, Pedro L.
author Garcia Guizzo, Melina
author_facet Garcia Guizzo, Melina
Parizi, Luís Fernando
Nunes, Rodrigo Dutra
Schama, Renata
Albano, Rodolpho M.
Tirloni, Lucas
Oldiges, Daiane Patrícia
Pilz Vieira, Ricardo
Cruz Oliveira, Wanderson Henrique
De Souza Leite, Milane
Gonzales, Sergio A.
Farber, Marisa Diana
Martins, Orlando
Da Silva Vaz, Itabajara
Oliveira, Pedro L.
author_role author
author2 Parizi, Luís Fernando
Nunes, Rodrigo Dutra
Schama, Renata
Albano, Rodolpho M.
Tirloni, Lucas
Oldiges, Daiane Patrícia
Pilz Vieira, Ricardo
Cruz Oliveira, Wanderson Henrique
De Souza Leite, Milane
Gonzales, Sergio A.
Farber, Marisa Diana
Martins, Orlando
Da Silva Vaz, Itabajara
Oliveira, Pedro L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APPLIED MICROBIOLOGY
SYMBIOSIS
METAGENOMICS
TICKS
topic APPLIED MICROBIOLOGY
SYMBIOSIS
METAGENOMICS
TICKS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts.
Fil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro; Brasil
Fil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Nunes, Rodrigo Dutra. Universidade Federal do Rio de Janeiro; Brasil
Fil: Schama, Renata. Laboratório de Biologia Computacional e Sistemas; Brasil
Fil: Albano, Rodolpho M.. Universidade do Estado do Rio de Janeiro; Brasil
Fil: Tirloni, Lucas. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Oldiges, Daiane Patrícia. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Pilz Vieira, Ricardo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Cruz Oliveira, Wanderson Henrique. Universidade Federal Rural do Rio de Janeiro; Brasil
Fil: De Souza Leite, Milane. Universidade Federal Rural do Rio de Janeiro; Brasil
Fil: Gonzales, Sergio A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Martins, Orlando. Universidade Federal do Rio de Janeiro; Brasil
Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
description The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41664
Garcia Guizzo, Melina; Parizi, Luís Fernando; Nunes, Rodrigo Dutra; Schama, Renata; Albano, Rodolpho M.; et al.; A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41664
identifier_str_mv Garcia Guizzo, Melina; Parizi, Luís Fernando; Nunes, Rodrigo Dutra; Schama, Renata; Albano, Rodolpho M.; et al.; A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-17309-x
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-17309-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270106777812992
score 13.13397

Publicaciones similares