Hepatotoxicity produced by paracetamol

• Autores: Moyano, Mario Franco; Pelzer, L.; Wendel, G.
• Año de publicación: 2021
• Idioma: inglés
• Tipo de recurso: documento de conferencia
• Materia: Aristolochia argentina; Antioxidant; Paracetamol; Hepatotoxicity
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Aristolochia argentina (Aristolochiaceae) is a rhizomatous plant popularly known as charrúa, charruga, patito,buche de pavo. The roots of this plant are used in popular medicine as: diuretic, antidiarrheic, astringent andemmenagogue. In previous phytochemical tests, the presence of flavonoid compounds in its infusion was verified. Theliver damage produced by paracetamol is a widely used model for the study of hepatoprotective substances that, whenmetabolized, produce free radicals that cause liver damage. A. argentina showed a high antioxidant activity of 265.8μmol of Fe (II)/g (FRAP). Aristolochia bracteolata Lam. and Aristolochia giberti possess antioxidant activity, too. Theaim of this research was to evaluate the effect of the root infusion of A. argentina on aspartate aminotransferase in theinjury produced by paracetamol. The plant material was collected in the province of San Luis. Root infusion preparedaccording to the Argentine Pharmacopoeia VI edition in concentrations of 10% of A. argentina was used. Wistar rats ofboth sexes, 150?180 g, were used. Liver injury was caused by oral administration of 640 mg/kg of paracetamol. Theexperimental animals (N = 6?8/group) were administered via oral 10 mL/kg/day (for 3 days) of physiological solution(group 1), physiological solution plus paracetamol one hour after the last dose (group 2) and infusion of A. argentina (for3 days) plus paracetamol one hour after the last dose (group 3). One day later, blood samples were obtained for thebiochemical determination of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline-phosphatase(ALP), γ-GT (gamma glutamyl transferase), total protein, and bilirubin. Biochemical determinations were carried outwith manual analysis techniques using Wiener lab reagents. Parametric ANOVA method was used. The toxic dose ofparacetamol (640 mg/kg) produced liver damage, as indicated by the significant increase in AST values compared to thecontrol group (62.17 ± 3.31 vs. 42.24 ± 4.03, P < 0.01). Pretreatment with the infusion of A. argentina producedsignificantly decreased AST activity (44.51 ± 2.45 vs. 62.17 ± 3.31, P < 0.01). No significant differences were found inthe values of ALT, γ-GT, total protein, and bilirubin (P > 0.05), (group 2 vs. group 3). In an oral repeated doses study ofA. argentina, no significant differences were found in AST, ALT, and total protein values. The results showed that theinfusion of A. argentina has a protective effect against paracetamol induced hepatotoxicity, evidenced by a decrease inthe enzymatic activity of AST. Future studies will be oriented towards the specific study of the mechanism of actioninvolved.
Fil: Moyano, Mario Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pelzer, L.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Wendel, G.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
XXXIX Reunión Científica Anual Sociedad de Biología de Cuyo
Argentina
Sociedad de Biología de Cuyo