Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity

Autores
Lardizábal, María Noelia; Rodriguez Virasoro, Ramiro Esteban; Nocito, Ana Lía; Daniele, Stella Maris; Palatnik, Javier Fernando; Veggi, Luis María
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
MicroRNAs are small RNA molecules that post-transcriptionally regulate gene expression. MicroRNA-122 is the most abundant and specific liver microRNA. Hepatotoxicity involves a significant alteration of liver gene expression. The aim of this work was to evaluate the microRNA-122 regulatory network in models of hepatotoxicity induced by thioacetamide or carbon tetrachloride. We report that the toxins decreased the expression of microRNA-122, which corresponded with an increase in two target genes: Cyclin G1 and the cationic amino acid transporter CAT-1. We found a decreased expression of its precursor, pri-microRNA-122, and of the transcription factors that specifically bind its promoter: CCAAT/enhancer-binding protein alpha, and members of the hepatocyte nuclear factor family. Therefore, microRNA-122 expression levels are under transcriptional control during hepatotoxicity. We propose that the changes observed are associated with the liver response to cope with the injury caused by the hepatotoxins, likely through a cell proliferation process to repair the damaged tissue.
Fil: Lardizábal, María Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Rodriguez Virasoro, Ramiro Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Nocito, Ana Lía. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
Fil: Daniele, Stella Maris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Veggi, Luis María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Rosario. Instituto de Fisiología Experimental (i); Argentina
Materia
Hepatotoxicity
Mir122
Microrna
Liver
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6030
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/6030
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicityLardizábal, María NoeliaRodriguez Virasoro, Ramiro EstebanNocito, Ana LíaDaniele, Stella MarisPalatnik, Javier FernandoVeggi, Luis MaríaHepatotoxicityMir122MicrornaLiverhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3MicroRNAs are small RNA molecules that post-transcriptionally regulate gene expression. MicroRNA-122 is the most abundant and specific liver microRNA. Hepatotoxicity involves a significant alteration of liver gene expression. The aim of this work was to evaluate the microRNA-122 regulatory network in models of hepatotoxicity induced by thioacetamide or carbon tetrachloride. We report that the toxins decreased the expression of microRNA-122, which corresponded with an increase in two target genes: Cyclin G1 and the cationic amino acid transporter CAT-1. We found a decreased expression of its precursor, pri-microRNA-122, and of the transcription factors that specifically bind its promoter: CCAAT/enhancer-binding protein alpha, and members of the hepatocyte nuclear factor family. Therefore, microRNA-122 expression levels are under transcriptional control during hepatotoxicity. We propose that the changes observed are associated with the liver response to cope with the injury caused by the hepatotoxins, likely through a cell proliferation process to repair the damaged tissue.Fil: Lardizábal, María Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Rosario. Instituto de Fisiología Experimental (i); ArgentinaFil: Rodriguez Virasoro, Ramiro Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Nocito, Ana Lía. Universidad Nacional de Rosario. Facultad de Cs.medicas; ArgentinaFil: Daniele, Stella Maris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Veggi, Luis María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Rosario. Instituto de Fisiología Experimental (i); ArgentinaElsevier Science2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6030Lardizábal, María Noelia; Rodriguez Virasoro, Ramiro Esteban; Nocito, Ana Lía; Daniele, Stella Maris; Palatnik, Javier Fernando; et al.; Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity; Elsevier Science; Environmental Toxicology and Pharmacology; 37; 1; 1-2014; 354-3641382-6689enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1382668913002809info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.etap.2013.12.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:39Zoai:ri.conicet.gov.ar:11336/6030instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:39.555CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity
title Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity
spellingShingle Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity
Lardizábal, María Noelia
Hepatotoxicity
Mir122
Microrna
Liver
title_short Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity
title_full Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity
title_fullStr Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity
title_full_unstemmed Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity
title_sort Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity
dc.creator.none.fl_str_mv Lardizábal, María Noelia
Rodriguez Virasoro, Ramiro Esteban
Nocito, Ana Lía
Daniele, Stella Maris
Palatnik, Javier Fernando
Veggi, Luis María
author Lardizábal, María Noelia
author_facet Lardizábal, María Noelia
Rodriguez Virasoro, Ramiro Esteban
Nocito, Ana Lía
Daniele, Stella Maris
Palatnik, Javier Fernando
Veggi, Luis María
author_role author
author2 Rodriguez Virasoro, Ramiro Esteban
Nocito, Ana Lía
Daniele, Stella Maris
Palatnik, Javier Fernando
Veggi, Luis María
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Hepatotoxicity
Mir122
Microrna
Liver
topic Hepatotoxicity
Mir122
Microrna
Liver
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv MicroRNAs are small RNA molecules that post-transcriptionally regulate gene expression. MicroRNA-122 is the most abundant and specific liver microRNA. Hepatotoxicity involves a significant alteration of liver gene expression. The aim of this work was to evaluate the microRNA-122 regulatory network in models of hepatotoxicity induced by thioacetamide or carbon tetrachloride. We report that the toxins decreased the expression of microRNA-122, which corresponded with an increase in two target genes: Cyclin G1 and the cationic amino acid transporter CAT-1. We found a decreased expression of its precursor, pri-microRNA-122, and of the transcription factors that specifically bind its promoter: CCAAT/enhancer-binding protein alpha, and members of the hepatocyte nuclear factor family. Therefore, microRNA-122 expression levels are under transcriptional control during hepatotoxicity. We propose that the changes observed are associated with the liver response to cope with the injury caused by the hepatotoxins, likely through a cell proliferation process to repair the damaged tissue.
Fil: Lardizábal, María Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Rodriguez Virasoro, Ramiro Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Nocito, Ana Lía. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
Fil: Daniele, Stella Maris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Veggi, Luis María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Rosario. Instituto de Fisiología Experimental (i); Argentina
description MicroRNAs are small RNA molecules that post-transcriptionally regulate gene expression. MicroRNA-122 is the most abundant and specific liver microRNA. Hepatotoxicity involves a significant alteration of liver gene expression. The aim of this work was to evaluate the microRNA-122 regulatory network in models of hepatotoxicity induced by thioacetamide or carbon tetrachloride. We report that the toxins decreased the expression of microRNA-122, which corresponded with an increase in two target genes: Cyclin G1 and the cationic amino acid transporter CAT-1. We found a decreased expression of its precursor, pri-microRNA-122, and of the transcription factors that specifically bind its promoter: CCAAT/enhancer-binding protein alpha, and members of the hepatocyte nuclear factor family. Therefore, microRNA-122 expression levels are under transcriptional control during hepatotoxicity. We propose that the changes observed are associated with the liver response to cope with the injury caused by the hepatotoxins, likely through a cell proliferation process to repair the damaged tissue.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6030
Lardizábal, María Noelia; Rodriguez Virasoro, Ramiro Esteban; Nocito, Ana Lía; Daniele, Stella Maris; Palatnik, Javier Fernando; et al.; Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity; Elsevier Science; Environmental Toxicology and Pharmacology; 37; 1; 1-2014; 354-364
1382-6689
url http://hdl.handle.net/11336/6030
identifier_str_mv Lardizábal, María Noelia; Rodriguez Virasoro, Ramiro Esteban; Nocito, Ana Lía; Daniele, Stella Maris; Palatnik, Javier Fernando; et al.; Alteration of the microRNA-122 regulatory network in rat models of hepatotoxicity; Elsevier Science; Environmental Toxicology and Pharmacology; 37; 1; 1-2014; 354-364
1382-6689
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1382668913002809
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.etap.2013.12.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613746564005888
score 13.070432

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