Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions

Autores
Philipp, Natalia; Gratton, Enrico; Estrada, Laura Cecilia
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cell membrane has a fundamental role in the cell life cycle but there’s still much to be learned about its heterogeneous structure, regulation, and protein interaction. Additionally, the protein-membrane interaction is often overlooked when studying specific protein dynamics. In this work, we present a new tool for a better understanding of protein dynamics and membrane function using live cells and fast non-invasive techniques without the need for individual particle tracking. To this end, we used the 2D-pair correlation function (2D-pCF) to study protein interactions across cellular membranes. We performed numerical simulations and confocal experiments using a GAP-mEGFP fusion construct known to interact with the plasmatic membrane. Our results demonstrate that based on a quantitative correlation analysis as the 2D pair correlation of the signal intensities, is possible to characterize protein-membrane interactions in live systems and real-time. Combining experimental and numerical results this work presents a new powerful approach to the study of the dynamic protein-membrane interaction.
Fil: Philipp, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Gratton, Enrico. University of California; Estados Unidos
Fil: Estrada, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Materia
FLUORESCENCE IMAGING
PROTEIN DYNAMICS
SPECTROSCOPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228029
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensionsPhilipp, NataliaGratton, EnricoEstrada, Laura CeciliaFLUORESCENCE IMAGINGPROTEIN DYNAMICSSPECTROSCOPYhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1The cell membrane has a fundamental role in the cell life cycle but there’s still much to be learned about its heterogeneous structure, regulation, and protein interaction. Additionally, the protein-membrane interaction is often overlooked when studying specific protein dynamics. In this work, we present a new tool for a better understanding of protein dynamics and membrane function using live cells and fast non-invasive techniques without the need for individual particle tracking. To this end, we used the 2D-pair correlation function (2D-pCF) to study protein interactions across cellular membranes. We performed numerical simulations and confocal experiments using a GAP-mEGFP fusion construct known to interact with the plasmatic membrane. Our results demonstrate that based on a quantitative correlation analysis as the 2D pair correlation of the signal intensities, is possible to characterize protein-membrane interactions in live systems and real-time. Combining experimental and numerical results this work presents a new powerful approach to the study of the dynamic protein-membrane interaction.Fil: Philipp, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Gratton, Enrico. University of California; Estados UnidosFil: Estrada, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaIOP Publishing2023-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228029Philipp, Natalia; Gratton, Enrico; Estrada, Laura Cecilia; Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions; IOP Publishing; Methods and Applications in Fluorescence; 11; 4; 8-2023; 1-152050-6120CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/2050-6120/acf118info:eu-repo/semantics/altIdentifier/doi/10.1088/2050-6120/acf118info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:52Zoai:ri.conicet.gov.ar:11336/228029instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:52.407CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions
title Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions
spellingShingle Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions
Philipp, Natalia
FLUORESCENCE IMAGING
PROTEIN DYNAMICS
SPECTROSCOPY
title_short Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions
title_full Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions
title_fullStr Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions
title_full_unstemmed Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions
title_sort Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions
dc.creator.none.fl_str_mv Philipp, Natalia
Gratton, Enrico
Estrada, Laura Cecilia
author Philipp, Natalia
author_facet Philipp, Natalia
Gratton, Enrico
Estrada, Laura Cecilia
author_role author
author2 Gratton, Enrico
Estrada, Laura Cecilia
author2_role author
author
dc.subject.none.fl_str_mv FLUORESCENCE IMAGING
PROTEIN DYNAMICS
SPECTROSCOPY
topic FLUORESCENCE IMAGING
PROTEIN DYNAMICS
SPECTROSCOPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The cell membrane has a fundamental role in the cell life cycle but there’s still much to be learned about its heterogeneous structure, regulation, and protein interaction. Additionally, the protein-membrane interaction is often overlooked when studying specific protein dynamics. In this work, we present a new tool for a better understanding of protein dynamics and membrane function using live cells and fast non-invasive techniques without the need for individual particle tracking. To this end, we used the 2D-pair correlation function (2D-pCF) to study protein interactions across cellular membranes. We performed numerical simulations and confocal experiments using a GAP-mEGFP fusion construct known to interact with the plasmatic membrane. Our results demonstrate that based on a quantitative correlation analysis as the 2D pair correlation of the signal intensities, is possible to characterize protein-membrane interactions in live systems and real-time. Combining experimental and numerical results this work presents a new powerful approach to the study of the dynamic protein-membrane interaction.
Fil: Philipp, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Gratton, Enrico. University of California; Estados Unidos
Fil: Estrada, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
description The cell membrane has a fundamental role in the cell life cycle but there’s still much to be learned about its heterogeneous structure, regulation, and protein interaction. Additionally, the protein-membrane interaction is often overlooked when studying specific protein dynamics. In this work, we present a new tool for a better understanding of protein dynamics and membrane function using live cells and fast non-invasive techniques without the need for individual particle tracking. To this end, we used the 2D-pair correlation function (2D-pCF) to study protein interactions across cellular membranes. We performed numerical simulations and confocal experiments using a GAP-mEGFP fusion construct known to interact with the plasmatic membrane. Our results demonstrate that based on a quantitative correlation analysis as the 2D pair correlation of the signal intensities, is possible to characterize protein-membrane interactions in live systems and real-time. Combining experimental and numerical results this work presents a new powerful approach to the study of the dynamic protein-membrane interaction.
publishDate 2023
dc.date.none.fl_str_mv 2023-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228029
Philipp, Natalia; Gratton, Enrico; Estrada, Laura Cecilia; Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions; IOP Publishing; Methods and Applications in Fluorescence; 11; 4; 8-2023; 1-15
2050-6120
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228029
identifier_str_mv Philipp, Natalia; Gratton, Enrico; Estrada, Laura Cecilia; Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions; IOP Publishing; Methods and Applications in Fluorescence; 11; 4; 8-2023; 1-15
2050-6120
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/2050-6120/acf118
info:eu-repo/semantics/altIdentifier/doi/10.1088/2050-6120/acf118
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv IOP Publishing
publisher.none.fl_str_mv IOP Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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