From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments

Autores
Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with others as they get transported. When these interactions occur faster than diffusion the resulting transport can be characterized by “effective diffusion coefficients” that depend on both the reaction rates and the “free” diffusion coefficients. Fluorescence correlation spectroscopy (FCS) gives information on effective rather than free diffusion coefficients under this condition. In the present paper we investigate what coefficients can be drawn from FCS experiments for a wide range of values of the ratio of reaction to diffusion time scales, using different fitting functions. We find that the effective coefficients can be inferred with relatively small errors even when the condition of fast reactions does not exactly hold. Since the diffusion time scale depends on the size of the observation volume and the reaction time scale depends on concentrations, we also discuss how by changing either one or the other property one can switch between the two limits and extract more information on the system under study.
Fil: Perez Ipiña, Emiliano. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
Materia
Fluorescence
Correlation
Spectroscopy
Diffusion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2492
Acceder
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spelling From free to effective diffusion coefficients in fluorescence correlation spectroscopy experimentsPerez Ipiña, EmilianoPonce Dawson, Silvina MarthaFluorescenceCorrelationSpectroscopyDiffusionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with others as they get transported. When these interactions occur faster than diffusion the resulting transport can be characterized by “effective diffusion coefficients” that depend on both the reaction rates and the “free” diffusion coefficients. Fluorescence correlation spectroscopy (FCS) gives information on effective rather than free diffusion coefficients under this condition. In the present paper we investigate what coefficients can be drawn from FCS experiments for a wide range of values of the ratio of reaction to diffusion time scales, using different fitting functions. We find that the effective coefficients can be inferred with relatively small errors even when the condition of fast reactions does not exactly hold. Since the diffusion time scale depends on the size of the observation volume and the reaction time scale depends on concentrations, we also discuss how by changing either one or the other property one can switch between the two limits and extract more information on the system under study.Fil: Perez Ipiña, Emiliano. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; ArgentinaFil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; ArgentinaAmerican Physical Society2013-02-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2492Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha; From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 87; 2; 13-2-2013; 22706-227061539-37551550-2376enginfo:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.87.022706info:eu-repo/semantics/altIdentifier/url/http://journals.aps.org/pre/abstract/10.1103/PhysRevE.87.022706info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:37:43Zoai:ri.conicet.gov.ar:11336/2492instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:37:43.353CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments
title From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments
spellingShingle From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments
Perez Ipiña, Emiliano
Fluorescence
Correlation
Spectroscopy
Diffusion
title_short From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments
title_full From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments
title_fullStr From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments
title_full_unstemmed From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments
title_sort From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments
dc.creator.none.fl_str_mv Perez Ipiña, Emiliano
Ponce Dawson, Silvina Martha
author Perez Ipiña, Emiliano
author_facet Perez Ipiña, Emiliano
Ponce Dawson, Silvina Martha
author_role author
author2 Ponce Dawson, Silvina Martha
author2_role author
dc.subject.none.fl_str_mv Fluorescence
Correlation
Spectroscopy
Diffusion
topic Fluorescence
Correlation
Spectroscopy
Diffusion
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with others as they get transported. When these interactions occur faster than diffusion the resulting transport can be characterized by “effective diffusion coefficients” that depend on both the reaction rates and the “free” diffusion coefficients. Fluorescence correlation spectroscopy (FCS) gives information on effective rather than free diffusion coefficients under this condition. In the present paper we investigate what coefficients can be drawn from FCS experiments for a wide range of values of the ratio of reaction to diffusion time scales, using different fitting functions. We find that the effective coefficients can be inferred with relatively small errors even when the condition of fast reactions does not exactly hold. Since the diffusion time scale depends on the size of the observation volume and the reaction time scale depends on concentrations, we also discuss how by changing either one or the other property one can switch between the two limits and extract more information on the system under study.
Fil: Perez Ipiña, Emiliano. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
description Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with others as they get transported. When these interactions occur faster than diffusion the resulting transport can be characterized by “effective diffusion coefficients” that depend on both the reaction rates and the “free” diffusion coefficients. Fluorescence correlation spectroscopy (FCS) gives information on effective rather than free diffusion coefficients under this condition. In the present paper we investigate what coefficients can be drawn from FCS experiments for a wide range of values of the ratio of reaction to diffusion time scales, using different fitting functions. We find that the effective coefficients can be inferred with relatively small errors even when the condition of fast reactions does not exactly hold. Since the diffusion time scale depends on the size of the observation volume and the reaction time scale depends on concentrations, we also discuss how by changing either one or the other property one can switch between the two limits and extract more information on the system under study.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2492
Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha; From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 87; 2; 13-2-2013; 22706-22706
1539-3755
1550-2376
url http://hdl.handle.net/11336/2492
identifier_str_mv Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha; From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 87; 2; 13-2-2013; 22706-22706
1539-3755
1550-2376
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.87.022706
info:eu-repo/semantics/altIdentifier/url/http://journals.aps.org/pre/abstract/10.1103/PhysRevE.87.022706
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physical Society
publisher.none.fl_str_mv American Physical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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