From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments
- Autores
- Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with others as they get transported. When these interactions occur faster than diffusion the resulting transport can be characterized by “effective diffusion coefficients” that depend on both the reaction rates and the “free” diffusion coefficients. Fluorescence correlation spectroscopy (FCS) gives information on effective rather than free diffusion coefficients under this condition. In the present paper we investigate what coefficients can be drawn from FCS experiments for a wide range of values of the ratio of reaction to diffusion time scales, using different fitting functions. We find that the effective coefficients can be inferred with relatively small errors even when the condition of fast reactions does not exactly hold. Since the diffusion time scale depends on the size of the observation volume and the reaction time scale depends on concentrations, we also discuss how by changing either one or the other property one can switch between the two limits and extract more information on the system under study.
Fil: Perez Ipiña, Emiliano. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina - Materia
-
Fluorescence
Correlation
Spectroscopy
Diffusion - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/2492
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From free to effective diffusion coefficients in fluorescence correlation spectroscopy experimentsPerez Ipiña, EmilianoPonce Dawson, Silvina MarthaFluorescenceCorrelationSpectroscopyDiffusionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with others as they get transported. When these interactions occur faster than diffusion the resulting transport can be characterized by “effective diffusion coefficients” that depend on both the reaction rates and the “free” diffusion coefficients. Fluorescence correlation spectroscopy (FCS) gives information on effective rather than free diffusion coefficients under this condition. In the present paper we investigate what coefficients can be drawn from FCS experiments for a wide range of values of the ratio of reaction to diffusion time scales, using different fitting functions. We find that the effective coefficients can be inferred with relatively small errors even when the condition of fast reactions does not exactly hold. Since the diffusion time scale depends on the size of the observation volume and the reaction time scale depends on concentrations, we also discuss how by changing either one or the other property one can switch between the two limits and extract more information on the system under study.Fil: Perez Ipiña, Emiliano. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; ArgentinaFil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; ArgentinaAmerican Physical Society2013-02-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2492Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha; From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 87; 2; 13-2-2013; 22706-227061539-37551550-2376enginfo:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.87.022706info:eu-repo/semantics/altIdentifier/url/http://journals.aps.org/pre/abstract/10.1103/PhysRevE.87.022706info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:43Zoai:ri.conicet.gov.ar:11336/2492instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:43.709CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments |
title |
From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments |
spellingShingle |
From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments Perez Ipiña, Emiliano Fluorescence Correlation Spectroscopy Diffusion |
title_short |
From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments |
title_full |
From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments |
title_fullStr |
From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments |
title_full_unstemmed |
From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments |
title_sort |
From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments |
dc.creator.none.fl_str_mv |
Perez Ipiña, Emiliano Ponce Dawson, Silvina Martha |
author |
Perez Ipiña, Emiliano |
author_facet |
Perez Ipiña, Emiliano Ponce Dawson, Silvina Martha |
author_role |
author |
author2 |
Ponce Dawson, Silvina Martha |
author2_role |
author |
dc.subject.none.fl_str_mv |
Fluorescence Correlation Spectroscopy Diffusion |
topic |
Fluorescence Correlation Spectroscopy Diffusion |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with others as they get transported. When these interactions occur faster than diffusion the resulting transport can be characterized by “effective diffusion coefficients” that depend on both the reaction rates and the “free” diffusion coefficients. Fluorescence correlation spectroscopy (FCS) gives information on effective rather than free diffusion coefficients under this condition. In the present paper we investigate what coefficients can be drawn from FCS experiments for a wide range of values of the ratio of reaction to diffusion time scales, using different fitting functions. We find that the effective coefficients can be inferred with relatively small errors even when the condition of fast reactions does not exactly hold. Since the diffusion time scale depends on the size of the observation volume and the reaction time scale depends on concentrations, we also discuss how by changing either one or the other property one can switch between the two limits and extract more information on the system under study. Fil: Perez Ipiña, Emiliano. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina |
description |
Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with others as they get transported. When these interactions occur faster than diffusion the resulting transport can be characterized by “effective diffusion coefficients” that depend on both the reaction rates and the “free” diffusion coefficients. Fluorescence correlation spectroscopy (FCS) gives information on effective rather than free diffusion coefficients under this condition. In the present paper we investigate what coefficients can be drawn from FCS experiments for a wide range of values of the ratio of reaction to diffusion time scales, using different fitting functions. We find that the effective coefficients can be inferred with relatively small errors even when the condition of fast reactions does not exactly hold. Since the diffusion time scale depends on the size of the observation volume and the reaction time scale depends on concentrations, we also discuss how by changing either one or the other property one can switch between the two limits and extract more information on the system under study. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-13 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/2492 Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha; From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 87; 2; 13-2-2013; 22706-22706 1539-3755 1550-2376 |
url |
http://hdl.handle.net/11336/2492 |
identifier_str_mv |
Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha; From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 87; 2; 13-2-2013; 22706-22706 1539-3755 1550-2376 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.87.022706 info:eu-repo/semantics/altIdentifier/url/http://journals.aps.org/pre/abstract/10.1103/PhysRevE.87.022706 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Physical Society |
publisher.none.fl_str_mv |
American Physical Society |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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