Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization

Autores
Herrero, Ornella Marisa; Alvarez, Hector Manuel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We investigated biomass and lipid production from whey by different Rhodococcus strains. The studied microorganisms exhibited different capability for growing and producing lipids from whey permeate. Strains belonging to R. opacus exhibited high yields of biomass (6.1–6.3 g/L) and lipid content (45–48% of CDW), whereas R. jostii, R. erythropolis, R. fascians, and R. equi strains produced low biomass (1.8–2.9 g/L) and lipids (lesser than 5% of CDW) from whey. Lactose and galactose, which are main constituents of whey, supported growth of R. opacus strains, but not of the other investigated species. A genome‐wide bioinformatic analyses demonstrated that some genes coding for transport systems (LacEFGK) and the β‐galactosidase (LacB) enzyme for lactose cleavage are lacking in such species, which may explain their inability to utilize lactose, galactose, and whey for an efficient biomass and lipid production. R. opacus possesses a complete genetic endowment for degrading lactose, galactose, and whey as well as for lipid biosynthesis from such substrates. Thus, R. opacus is a robust candidate for single‐cell oil production from whey. The cultivation of R. opacus cells on crude whey resulted in an increase of lipid production from 3.0 to 6.4 g/L, in comparison to whey permeate. Practical application: This study demonstrates that the bioconversion of whey to oils by Rhodococcus strains is feasible. However, we found some genetic and physiological differences for whey, lactose and galactose catabolism and assimilation among rhodococcal species. Our results demonstrate that among five different rhodococcal species, Rhodococcus opacus has the more robust genetic endowment for supporting high yields of biomass and lipid production from whey. The use of whey for single cell oil production by rhodococci may serve as platform for developing environmentally friendly biotechnological processes.
Fil: Herrero, Ornella Marisa. Oil M And S; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, Hector Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina
Materia
GALACTOSE
LACTOSE
TRIACYLGLYCEROLS
WHEY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94620
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94620
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilizationHerrero, Ornella MarisaAlvarez, Hector ManuelGALACTOSELACTOSETRIACYLGLYCEROLSWHEYhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2We investigated biomass and lipid production from whey by different Rhodococcus strains. The studied microorganisms exhibited different capability for growing and producing lipids from whey permeate. Strains belonging to R. opacus exhibited high yields of biomass (6.1–6.3 g/L) and lipid content (45–48% of CDW), whereas R. jostii, R. erythropolis, R. fascians, and R. equi strains produced low biomass (1.8–2.9 g/L) and lipids (lesser than 5% of CDW) from whey. Lactose and galactose, which are main constituents of whey, supported growth of R. opacus strains, but not of the other investigated species. A genome‐wide bioinformatic analyses demonstrated that some genes coding for transport systems (LacEFGK) and the β‐galactosidase (LacB) enzyme for lactose cleavage are lacking in such species, which may explain their inability to utilize lactose, galactose, and whey for an efficient biomass and lipid production. R. opacus possesses a complete genetic endowment for degrading lactose, galactose, and whey as well as for lipid biosynthesis from such substrates. Thus, R. opacus is a robust candidate for single‐cell oil production from whey. The cultivation of R. opacus cells on crude whey resulted in an increase of lipid production from 3.0 to 6.4 g/L, in comparison to whey permeate. Practical application: This study demonstrates that the bioconversion of whey to oils by Rhodococcus strains is feasible. However, we found some genetic and physiological differences for whey, lactose and galactose catabolism and assimilation among rhodococcal species. Our results demonstrate that among five different rhodococcal species, Rhodococcus opacus has the more robust genetic endowment for supporting high yields of biomass and lipid production from whey. The use of whey for single cell oil production by rhodococci may serve as platform for developing environmentally friendly biotechnological processes.Fil: Herrero, Ornella Marisa. Oil M And S; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarez, Hector Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; ArgentinaWiley VCH Verlag2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94620Herrero, Ornella Marisa; Alvarez, Hector Manuel; Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization; Wiley VCH Verlag; European Journal of Lipid Science and Technology; 118; 2; 2-2016; 262-2721438-7697CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/ejlt.201500080info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ejlt.201500080info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:15Zoai:ri.conicet.gov.ar:11336/94620instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:15.704CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization
title Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization
spellingShingle Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization
Herrero, Ornella Marisa
GALACTOSE
LACTOSE
TRIACYLGLYCEROLS
WHEY
title_short Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization
title_full Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization
title_fullStr Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization
title_full_unstemmed Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization
title_sort Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization
dc.creator.none.fl_str_mv Herrero, Ornella Marisa
Alvarez, Hector Manuel
author Herrero, Ornella Marisa
author_facet Herrero, Ornella Marisa
Alvarez, Hector Manuel
author_role author
author2 Alvarez, Hector Manuel
author2_role author
dc.subject.none.fl_str_mv GALACTOSE
LACTOSE
TRIACYLGLYCEROLS
WHEY
topic GALACTOSE
LACTOSE
TRIACYLGLYCEROLS
WHEY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv We investigated biomass and lipid production from whey by different Rhodococcus strains. The studied microorganisms exhibited different capability for growing and producing lipids from whey permeate. Strains belonging to R. opacus exhibited high yields of biomass (6.1–6.3 g/L) and lipid content (45–48% of CDW), whereas R. jostii, R. erythropolis, R. fascians, and R. equi strains produced low biomass (1.8–2.9 g/L) and lipids (lesser than 5% of CDW) from whey. Lactose and galactose, which are main constituents of whey, supported growth of R. opacus strains, but not of the other investigated species. A genome‐wide bioinformatic analyses demonstrated that some genes coding for transport systems (LacEFGK) and the β‐galactosidase (LacB) enzyme for lactose cleavage are lacking in such species, which may explain their inability to utilize lactose, galactose, and whey for an efficient biomass and lipid production. R. opacus possesses a complete genetic endowment for degrading lactose, galactose, and whey as well as for lipid biosynthesis from such substrates. Thus, R. opacus is a robust candidate for single‐cell oil production from whey. The cultivation of R. opacus cells on crude whey resulted in an increase of lipid production from 3.0 to 6.4 g/L, in comparison to whey permeate. Practical application: This study demonstrates that the bioconversion of whey to oils by Rhodococcus strains is feasible. However, we found some genetic and physiological differences for whey, lactose and galactose catabolism and assimilation among rhodococcal species. Our results demonstrate that among five different rhodococcal species, Rhodococcus opacus has the more robust genetic endowment for supporting high yields of biomass and lipid production from whey. The use of whey for single cell oil production by rhodococci may serve as platform for developing environmentally friendly biotechnological processes.
Fil: Herrero, Ornella Marisa. Oil M And S; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, Hector Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina
description We investigated biomass and lipid production from whey by different Rhodococcus strains. The studied microorganisms exhibited different capability for growing and producing lipids from whey permeate. Strains belonging to R. opacus exhibited high yields of biomass (6.1–6.3 g/L) and lipid content (45–48% of CDW), whereas R. jostii, R. erythropolis, R. fascians, and R. equi strains produced low biomass (1.8–2.9 g/L) and lipids (lesser than 5% of CDW) from whey. Lactose and galactose, which are main constituents of whey, supported growth of R. opacus strains, but not of the other investigated species. A genome‐wide bioinformatic analyses demonstrated that some genes coding for transport systems (LacEFGK) and the β‐galactosidase (LacB) enzyme for lactose cleavage are lacking in such species, which may explain their inability to utilize lactose, galactose, and whey for an efficient biomass and lipid production. R. opacus possesses a complete genetic endowment for degrading lactose, galactose, and whey as well as for lipid biosynthesis from such substrates. Thus, R. opacus is a robust candidate for single‐cell oil production from whey. The cultivation of R. opacus cells on crude whey resulted in an increase of lipid production from 3.0 to 6.4 g/L, in comparison to whey permeate. Practical application: This study demonstrates that the bioconversion of whey to oils by Rhodococcus strains is feasible. However, we found some genetic and physiological differences for whey, lactose and galactose catabolism and assimilation among rhodococcal species. Our results demonstrate that among five different rhodococcal species, Rhodococcus opacus has the more robust genetic endowment for supporting high yields of biomass and lipid production from whey. The use of whey for single cell oil production by rhodococci may serve as platform for developing environmentally friendly biotechnological processes.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94620
Herrero, Ornella Marisa; Alvarez, Hector Manuel; Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization; Wiley VCH Verlag; European Journal of Lipid Science and Technology; 118; 2; 2-2016; 262-272
1438-7697
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94620
identifier_str_mv Herrero, Ornella Marisa; Alvarez, Hector Manuel; Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization; Wiley VCH Verlag; European Journal of Lipid Science and Technology; 118; 2; 2-2016; 262-272
1438-7697
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/ejlt.201500080
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ejlt.201500080
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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