Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells

Autores
de Gregorio, Priscilla Romina; Salva, Maria Susana; Juárez Tomás, María Silvina; Nader, Maria Elena Fatima
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sex hormones are oft en administered to mice in vaginal microbial studies in order to synchronize estrous. Our objective was to evaluate the effect of estradiol-hemi succinate (EH) or medroxyprogest erone-acetate (MPA) administration on estrous cycle, vaginal microbiota, and immune cell populations of various organs in a murine model. Two-month-old female BALB/cmice were intramuscularly injected with EH (day -2) or MPA (day -5) to induce estrous (E) or diestrous (DE), respectively. On sampling days (Sd) 0, 2, 6 and 8, vaginal washings (v.w.), vagina, blood, spleen and bone marrow (BM) samples were taken. Most of the animals remained in E or DE states until Sd 6 after EH or MPA administration, respectively. The numberof cultivable vaginal bacteria was not modified by hormonal treatments; higher quantities weredetected in mice in E. Only EH administration modifi ed serum sex hormone levels, increasing serum estradiol on Sd 0. In v.w., myeloid population was dominant while lymphoid populations were not detected. Only MPA administration induced a reduction in myeloid cells on Sd 0.Hormonal treatments did not aff ect myeloid populations in BM but caused a slight decrease in T and B cells. In spleen, hormonal administration did not aff ect B or T population size while an increase in mature B cells and a decrease in immature B cells were observed in MPA-treated mice compared with EH-treated mice. Th us, although both hormonal treatments induced slight changes in some of the parameters evaluated compared to control animals, adequately standardized and consistent experimental protocols were established for further studies.
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Vaginal Lactobacilli
Sex Hormones
Vaginal Tract
Immune Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82344
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/82344
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cellsde Gregorio, Priscilla RominaSalva, Maria SusanaJuárez Tomás, María SilvinaNader, Maria Elena FatimaVaginal LactobacilliSex HormonesVaginal TractImmune Cellshttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Sex hormones are oft en administered to mice in vaginal microbial studies in order to synchronize estrous. Our objective was to evaluate the effect of estradiol-hemi succinate (EH) or medroxyprogest erone-acetate (MPA) administration on estrous cycle, vaginal microbiota, and immune cell populations of various organs in a murine model. Two-month-old female BALB/cmice were intramuscularly injected with EH (day -2) or MPA (day -5) to induce estrous (E) or diestrous (DE), respectively. On sampling days (Sd) 0, 2, 6 and 8, vaginal washings (v.w.), vagina, blood, spleen and bone marrow (BM) samples were taken. Most of the animals remained in E or DE states until Sd 6 after EH or MPA administration, respectively. The numberof cultivable vaginal bacteria was not modified by hormonal treatments; higher quantities weredetected in mice in E. Only EH administration modifi ed serum sex hormone levels, increasing serum estradiol on Sd 0. In v.w., myeloid population was dominant while lymphoid populations were not detected. Only MPA administration induced a reduction in myeloid cells on Sd 0.Hormonal treatments did not aff ect myeloid populations in BM but caused a slight decrease in T and B cells. In spleen, hormonal administration did not aff ect B or T population size while an increase in mature B cells and a decrease in immature B cells were observed in MPA-treated mice compared with EH-treated mice. Th us, although both hormonal treatments induced slight changes in some of the parameters evaluated compared to control animals, adequately standardized and consistent experimental protocols were established for further studies.Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaScandinavian Society for Laboratory Animal Science2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82344de Gregorio, Priscilla Romina; Salva, Maria Susana; Juárez Tomás, María Silvina; Nader, Maria Elena Fatima; Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells; Scandinavian Society for Laboratory Animal Science; Scandinavian Journal of Laboratory Animal Science; 44; 3; 6-2018; 1-142002-0112CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sjlas.org/index.php/SJLAS/article/view/526info:eu-repo/semantics/altIdentifier/doi/10.23675/sjlas.v44i0.526info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:56Zoai:ri.conicet.gov.ar:11336/82344instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:56.48CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells
title Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells
spellingShingle Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells
de Gregorio, Priscilla Romina
Vaginal Lactobacilli
Sex Hormones
Vaginal Tract
Immune Cells
title_short Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells
title_full Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells
title_fullStr Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells
title_full_unstemmed Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells
title_sort Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells
dc.creator.none.fl_str_mv de Gregorio, Priscilla Romina
Salva, Maria Susana
Juárez Tomás, María Silvina
Nader, Maria Elena Fatima
author de Gregorio, Priscilla Romina
author_facet de Gregorio, Priscilla Romina
Salva, Maria Susana
Juárez Tomás, María Silvina
Nader, Maria Elena Fatima
author_role author
author2 Salva, Maria Susana
Juárez Tomás, María Silvina
Nader, Maria Elena Fatima
author2_role author
author
author
dc.subject.none.fl_str_mv Vaginal Lactobacilli
Sex Hormones
Vaginal Tract
Immune Cells
topic Vaginal Lactobacilli
Sex Hormones
Vaginal Tract
Immune Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Sex hormones are oft en administered to mice in vaginal microbial studies in order to synchronize estrous. Our objective was to evaluate the effect of estradiol-hemi succinate (EH) or medroxyprogest erone-acetate (MPA) administration on estrous cycle, vaginal microbiota, and immune cell populations of various organs in a murine model. Two-month-old female BALB/cmice were intramuscularly injected with EH (day -2) or MPA (day -5) to induce estrous (E) or diestrous (DE), respectively. On sampling days (Sd) 0, 2, 6 and 8, vaginal washings (v.w.), vagina, blood, spleen and bone marrow (BM) samples were taken. Most of the animals remained in E or DE states until Sd 6 after EH or MPA administration, respectively. The numberof cultivable vaginal bacteria was not modified by hormonal treatments; higher quantities weredetected in mice in E. Only EH administration modifi ed serum sex hormone levels, increasing serum estradiol on Sd 0. In v.w., myeloid population was dominant while lymphoid populations were not detected. Only MPA administration induced a reduction in myeloid cells on Sd 0.Hormonal treatments did not aff ect myeloid populations in BM but caused a slight decrease in T and B cells. In spleen, hormonal administration did not aff ect B or T population size while an increase in mature B cells and a decrease in immature B cells were observed in MPA-treated mice compared with EH-treated mice. Th us, although both hormonal treatments induced slight changes in some of the parameters evaluated compared to control animals, adequately standardized and consistent experimental protocols were established for further studies.
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Sex hormones are oft en administered to mice in vaginal microbial studies in order to synchronize estrous. Our objective was to evaluate the effect of estradiol-hemi succinate (EH) or medroxyprogest erone-acetate (MPA) administration on estrous cycle, vaginal microbiota, and immune cell populations of various organs in a murine model. Two-month-old female BALB/cmice were intramuscularly injected with EH (day -2) or MPA (day -5) to induce estrous (E) or diestrous (DE), respectively. On sampling days (Sd) 0, 2, 6 and 8, vaginal washings (v.w.), vagina, blood, spleen and bone marrow (BM) samples were taken. Most of the animals remained in E or DE states until Sd 6 after EH or MPA administration, respectively. The numberof cultivable vaginal bacteria was not modified by hormonal treatments; higher quantities weredetected in mice in E. Only EH administration modifi ed serum sex hormone levels, increasing serum estradiol on Sd 0. In v.w., myeloid population was dominant while lymphoid populations were not detected. Only MPA administration induced a reduction in myeloid cells on Sd 0.Hormonal treatments did not aff ect myeloid populations in BM but caused a slight decrease in T and B cells. In spleen, hormonal administration did not aff ect B or T population size while an increase in mature B cells and a decrease in immature B cells were observed in MPA-treated mice compared with EH-treated mice. Th us, although both hormonal treatments induced slight changes in some of the parameters evaluated compared to control animals, adequately standardized and consistent experimental protocols were established for further studies.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82344
de Gregorio, Priscilla Romina; Salva, Maria Susana; Juárez Tomás, María Silvina; Nader, Maria Elena Fatima; Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells; Scandinavian Society for Laboratory Animal Science; Scandinavian Journal of Laboratory Animal Science; 44; 3; 6-2018; 1-14
2002-0112
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82344
identifier_str_mv de Gregorio, Priscilla Romina; Salva, Maria Susana; Juárez Tomás, María Silvina; Nader, Maria Elena Fatima; Effects of exogenous sex hormones on mouse estrous cycle, vaginal microbiota and immune cells; Scandinavian Society for Laboratory Animal Science; Scandinavian Journal of Laboratory Animal Science; 44; 3; 6-2018; 1-14
2002-0112
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sjlas.org/index.php/SJLAS/article/view/526
info:eu-repo/semantics/altIdentifier/doi/10.23675/sjlas.v44i0.526
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scandinavian Society for Laboratory Animal Science
publisher.none.fl_str_mv Scandinavian Society for Laboratory Animal Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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