Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications

Autores
Juárez Tomás, María Silvina; de Gregorio, Priscilla Romina; Leccese Terraf, Maria Cecilia; Nader, Maria Elena Fatima
Año de publicación
2015
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
One of the main challenges during the design of pharmabiotic products is that the select microorganisms resist to the processes of biomass production, combination in the final product and storage during the shelf life period. In this work, the resistance of biofilm-forming vaginal Lactobacillus reuteri CRL 1324 to encapsulation, freeze-drying and storage were assayed. L. reuteri was encapsulated applying the extrusion-ionic gelation technique, using microbial polymers (1% xanthan gum-0.75% gellan gum). Capsule aliquots were freeze-dried with or without lyoprotectors (12% lactose-6% skim milk). Capsules were stored at room and refrigeration conditions for 150 days. The following evaluations were performed: viability of bioactive ingredient (i.e. viable L. reuteri) before and after encapsulation, freeze-drying and storage; microorganism release and resistance to the genital tract conditions in a medium simulating the vaginal fluid (pH = 4.2), and maintenance of the beneficial properties (biofilm formation and Streptococcus agalactiae NH17 inhibition). High yield of capsules, high encapsulation efficiency and viable entrapped lactobacilli were obtained. The resistance of encapsulated L. reuteri to lyophilization was higher with lactose/milk. At 150 days of storage, viable cells from freeze-dried capsules (with or without lyoprotectors) stored at refrigeration temperature were only recovered. From the optimal system (lyophilized capsules with lyoprotectors), L. reuteri was released in a culture medium simulating vaginal fluid, maintaining its viability during 24 h at 37°C and the capability to form biofilm and to inhibit S. agalactiae NH17 growth. In conclusion, the combination of encapsulation (by extrusion-ionic gelation) and freeze-drying processes in presence of lyoprotectors, and the subsequent storage at refrigeration conditions favored the maintenance of L. reuteri CRL 1324 viability and functionality. Encapsulated and freeze-dried beneficial lactobacilli can then be included in a suitable pharmaceutical form for vaginal application, to prevent or treat female urogenital infections.
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
3rd International Meeting on Pharmaceutical Sciences
Córdoba
Argentina
Universidad Nacional de Córdoba. Facultad de Ciencias Químicas
Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
Materia
VAGINAL LACTOBACILLI
ENCAPSULATION
PHARMABIOTICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263346
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/263346
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applicationsJuárez Tomás, María Silvinade Gregorio, Priscilla RominaLeccese Terraf, Maria CeciliaNader, Maria Elena FatimaVAGINAL LACTOBACILLIENCAPSULATIONPHARMABIOTICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1One of the main challenges during the design of pharmabiotic products is that the select microorganisms resist to the processes of biomass production, combination in the final product and storage during the shelf life period. In this work, the resistance of biofilm-forming vaginal Lactobacillus reuteri CRL 1324 to encapsulation, freeze-drying and storage were assayed. L. reuteri was encapsulated applying the extrusion-ionic gelation technique, using microbial polymers (1% xanthan gum-0.75% gellan gum). Capsule aliquots were freeze-dried with or without lyoprotectors (12% lactose-6% skim milk). Capsules were stored at room and refrigeration conditions for 150 days. The following evaluations were performed: viability of bioactive ingredient (i.e. viable L. reuteri) before and after encapsulation, freeze-drying and storage; microorganism release and resistance to the genital tract conditions in a medium simulating the vaginal fluid (pH = 4.2), and maintenance of the beneficial properties (biofilm formation and Streptococcus agalactiae NH17 inhibition). High yield of capsules, high encapsulation efficiency and viable entrapped lactobacilli were obtained. The resistance of encapsulated L. reuteri to lyophilization was higher with lactose/milk. At 150 days of storage, viable cells from freeze-dried capsules (with or without lyoprotectors) stored at refrigeration temperature were only recovered. From the optimal system (lyophilized capsules with lyoprotectors), L. reuteri was released in a culture medium simulating vaginal fluid, maintaining its viability during 24 h at 37°C and the capability to form biofilm and to inhibit S. agalactiae NH17 growth. In conclusion, the combination of encapsulation (by extrusion-ionic gelation) and freeze-drying processes in presence of lyoprotectors, and the subsequent storage at refrigeration conditions favored the maintenance of L. reuteri CRL 1324 viability and functionality. Encapsulated and freeze-dried beneficial lactobacilli can then be included in a suitable pharmaceutical form for vaginal application, to prevent or treat female urogenital infections.Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina3rd International Meeting on Pharmaceutical SciencesCórdobaArgentinaUniversidad Nacional de Córdoba. Facultad de Ciencias QuímicasUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y FarmacéuticasSociety of Pharmaceutical Sciences and Research2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263346Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications; 3rd International Meeting on Pharmaceutical Sciences; Córdoba; Argentina; 2014; 88-882320-51480975-8232CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ijpsr.com/conference-proceeding/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:11Zoai:ri.conicet.gov.ar:11336/263346instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:11.43CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications
title Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications
spellingShingle Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications
Juárez Tomás, María Silvina
VAGINAL LACTOBACILLI
ENCAPSULATION
PHARMABIOTICS
title_short Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications
title_full Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications
title_fullStr Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications
title_full_unstemmed Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications
title_sort Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications
dc.creator.none.fl_str_mv Juárez Tomás, María Silvina
de Gregorio, Priscilla Romina
Leccese Terraf, Maria Cecilia
Nader, Maria Elena Fatima
author Juárez Tomás, María Silvina
author_facet Juárez Tomás, María Silvina
de Gregorio, Priscilla Romina
Leccese Terraf, Maria Cecilia
Nader, Maria Elena Fatima
author_role author
author2 de Gregorio, Priscilla Romina
Leccese Terraf, Maria Cecilia
Nader, Maria Elena Fatima
author2_role author
author
author
dc.subject.none.fl_str_mv VAGINAL LACTOBACILLI
ENCAPSULATION
PHARMABIOTICS
topic VAGINAL LACTOBACILLI
ENCAPSULATION
PHARMABIOTICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv One of the main challenges during the design of pharmabiotic products is that the select microorganisms resist to the processes of biomass production, combination in the final product and storage during the shelf life period. In this work, the resistance of biofilm-forming vaginal Lactobacillus reuteri CRL 1324 to encapsulation, freeze-drying and storage were assayed. L. reuteri was encapsulated applying the extrusion-ionic gelation technique, using microbial polymers (1% xanthan gum-0.75% gellan gum). Capsule aliquots were freeze-dried with or without lyoprotectors (12% lactose-6% skim milk). Capsules were stored at room and refrigeration conditions for 150 days. The following evaluations were performed: viability of bioactive ingredient (i.e. viable L. reuteri) before and after encapsulation, freeze-drying and storage; microorganism release and resistance to the genital tract conditions in a medium simulating the vaginal fluid (pH = 4.2), and maintenance of the beneficial properties (biofilm formation and Streptococcus agalactiae NH17 inhibition). High yield of capsules, high encapsulation efficiency and viable entrapped lactobacilli were obtained. The resistance of encapsulated L. reuteri to lyophilization was higher with lactose/milk. At 150 days of storage, viable cells from freeze-dried capsules (with or without lyoprotectors) stored at refrigeration temperature were only recovered. From the optimal system (lyophilized capsules with lyoprotectors), L. reuteri was released in a culture medium simulating vaginal fluid, maintaining its viability during 24 h at 37°C and the capability to form biofilm and to inhibit S. agalactiae NH17 growth. In conclusion, the combination of encapsulation (by extrusion-ionic gelation) and freeze-drying processes in presence of lyoprotectors, and the subsequent storage at refrigeration conditions favored the maintenance of L. reuteri CRL 1324 viability and functionality. Encapsulated and freeze-dried beneficial lactobacilli can then be included in a suitable pharmaceutical form for vaginal application, to prevent or treat female urogenital infections.
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
3rd International Meeting on Pharmaceutical Sciences
Córdoba
Argentina
Universidad Nacional de Córdoba. Facultad de Ciencias Químicas
Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
description One of the main challenges during the design of pharmabiotic products is that the select microorganisms resist to the processes of biomass production, combination in the final product and storage during the shelf life period. In this work, the resistance of biofilm-forming vaginal Lactobacillus reuteri CRL 1324 to encapsulation, freeze-drying and storage were assayed. L. reuteri was encapsulated applying the extrusion-ionic gelation technique, using microbial polymers (1% xanthan gum-0.75% gellan gum). Capsule aliquots were freeze-dried with or without lyoprotectors (12% lactose-6% skim milk). Capsules were stored at room and refrigeration conditions for 150 days. The following evaluations were performed: viability of bioactive ingredient (i.e. viable L. reuteri) before and after encapsulation, freeze-drying and storage; microorganism release and resistance to the genital tract conditions in a medium simulating the vaginal fluid (pH = 4.2), and maintenance of the beneficial properties (biofilm formation and Streptococcus agalactiae NH17 inhibition). High yield of capsules, high encapsulation efficiency and viable entrapped lactobacilli were obtained. The resistance of encapsulated L. reuteri to lyophilization was higher with lactose/milk. At 150 days of storage, viable cells from freeze-dried capsules (with or without lyoprotectors) stored at refrigeration temperature were only recovered. From the optimal system (lyophilized capsules with lyoprotectors), L. reuteri was released in a culture medium simulating vaginal fluid, maintaining its viability during 24 h at 37°C and the capability to form biofilm and to inhibit S. agalactiae NH17 growth. In conclusion, the combination of encapsulation (by extrusion-ionic gelation) and freeze-drying processes in presence of lyoprotectors, and the subsequent storage at refrigeration conditions favored the maintenance of L. reuteri CRL 1324 viability and functionality. Encapsulated and freeze-dried beneficial lactobacilli can then be included in a suitable pharmaceutical form for vaginal application, to prevent or treat female urogenital infections.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263346
Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications; 3rd International Meeting on Pharmaceutical Sciences; Córdoba; Argentina; 2014; 88-88
2320-5148
0975-8232
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263346
identifier_str_mv Encapsulation and subsequent freeze-drying of beneficial lactobacilli for their potential inclusion in pharmabiotic formulations for vaginal applications; 3rd International Meeting on Pharmaceutical Sciences; Córdoba; Argentina; 2014; 88-88
2320-5148
0975-8232
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ijpsr.com/conference-proceeding/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Society of Pharmaceutical Sciences and Research
publisher.none.fl_str_mv Society of Pharmaceutical Sciences and Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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