Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model

Autores
de Gregorio, Priscilla Romina; Juárez Tomás, María Silvina; Leccese Terraf, Maria Cecilia; Nader, Maria Elena Fatima
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
AIMS: To assess the preventive effect of different intravaginal (i.va.) doses of Lactobacillus reuteri CRL1324 against vaginal colonization by Group B Streptococcus (GBS) in a murine experimental model. METHODS AND RESULTS: The major virulence factors of four vaginal GBS clinical isolates were determined to select the most virulent strain and set up a murine model of streptococcal vaginal colonization. Later, the effect of four and seven doses of 10(8) viable cells of Lact. reuteri CRL1324 i.va. administered, prior to the GBS challenge was studied. Seven doses of lactobacilli were able to significantly reduce the number of viable GBS cells, while four doses showed no preventive effect. Both doses reduced the leucocyte influx induced by GBS. Seven doses caused a slight increase in the Lact. reuteri CRL1324 vaginal colonization compared with four doses and reduced murine vaginal pH compared to control mice. CONCLUSIONS: Lactobacillus reuteri CRL1324 evidenced a preventive effect on GBS vaginal colonization in an experimental mouse model. SIGNIFICANCE AND IMPACTS OF THE STUDY: Maternal GBS colonization is one of the most important risk factors for developing disease in newborns. Lactobacillus reuteri CRL1324 could be considered as a new biological agent to reduce infections caused by this micro-organism.
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Materia
Group B Streptococcus
Lactobacilli
Mouse Experimental Model
Preventive Effect
Urogenital Tract
Vaginal Probiotic
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28598
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/28598
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse modelde Gregorio, Priscilla RominaJuárez Tomás, María SilvinaLeccese Terraf, Maria CeciliaNader, Maria Elena FatimaGroup B StreptococcusLactobacilliMouse Experimental ModelPreventive EffectUrogenital TractVaginal Probiotichttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1AIMS: To assess the preventive effect of different intravaginal (i.va.) doses of Lactobacillus reuteri CRL1324 against vaginal colonization by Group B Streptococcus (GBS) in a murine experimental model. METHODS AND RESULTS: The major virulence factors of four vaginal GBS clinical isolates were determined to select the most virulent strain and set up a murine model of streptococcal vaginal colonization. Later, the effect of four and seven doses of 10(8) viable cells of Lact. reuteri CRL1324 i.va. administered, prior to the GBS challenge was studied. Seven doses of lactobacilli were able to significantly reduce the number of viable GBS cells, while four doses showed no preventive effect. Both doses reduced the leucocyte influx induced by GBS. Seven doses caused a slight increase in the Lact. reuteri CRL1324 vaginal colonization compared with four doses and reduced murine vaginal pH compared to control mice. CONCLUSIONS: Lactobacillus reuteri CRL1324 evidenced a preventive effect on GBS vaginal colonization in an experimental mouse model. SIGNIFICANCE AND IMPACTS OF THE STUDY: Maternal GBS colonization is one of the most important risk factors for developing disease in newborns. Lactobacillus reuteri CRL1324 could be considered as a new biological agent to reduce infections caused by this micro-organism.Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaWiley2015-01-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28598de Gregorio, Priscilla Romina; Juárez Tomás, María Silvina; Leccese Terraf, Maria Cecilia; Nader, Maria Elena Fatima; Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model; Wiley; Journal of Applied Microbiology; 118; 4; 29-1-2015; 1034-10471364-50721365-2672CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12739info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12739/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:13:15Zoai:ri.conicet.gov.ar:11336/28598instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:13:15.931CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model
title Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model
spellingShingle Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model
de Gregorio, Priscilla Romina
Group B Streptococcus
Lactobacilli
Mouse Experimental Model
Preventive Effect
Urogenital Tract
Vaginal Probiotic
title_short Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model
title_full Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model
title_fullStr Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model
title_full_unstemmed Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model
title_sort Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model
dc.creator.none.fl_str_mv de Gregorio, Priscilla Romina
Juárez Tomás, María Silvina
Leccese Terraf, Maria Cecilia
Nader, Maria Elena Fatima
author de Gregorio, Priscilla Romina
author_facet de Gregorio, Priscilla Romina
Juárez Tomás, María Silvina
Leccese Terraf, Maria Cecilia
Nader, Maria Elena Fatima
author_role author
author2 Juárez Tomás, María Silvina
Leccese Terraf, Maria Cecilia
Nader, Maria Elena Fatima
author2_role author
author
author
dc.subject.none.fl_str_mv Group B Streptococcus
Lactobacilli
Mouse Experimental Model
Preventive Effect
Urogenital Tract
Vaginal Probiotic
topic Group B Streptococcus
Lactobacilli
Mouse Experimental Model
Preventive Effect
Urogenital Tract
Vaginal Probiotic
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv AIMS: To assess the preventive effect of different intravaginal (i.va.) doses of Lactobacillus reuteri CRL1324 against vaginal colonization by Group B Streptococcus (GBS) in a murine experimental model. METHODS AND RESULTS: The major virulence factors of four vaginal GBS clinical isolates were determined to select the most virulent strain and set up a murine model of streptococcal vaginal colonization. Later, the effect of four and seven doses of 10(8) viable cells of Lact. reuteri CRL1324 i.va. administered, prior to the GBS challenge was studied. Seven doses of lactobacilli were able to significantly reduce the number of viable GBS cells, while four doses showed no preventive effect. Both doses reduced the leucocyte influx induced by GBS. Seven doses caused a slight increase in the Lact. reuteri CRL1324 vaginal colonization compared with four doses and reduced murine vaginal pH compared to control mice. CONCLUSIONS: Lactobacillus reuteri CRL1324 evidenced a preventive effect on GBS vaginal colonization in an experimental mouse model. SIGNIFICANCE AND IMPACTS OF THE STUDY: Maternal GBS colonization is one of the most important risk factors for developing disease in newborns. Lactobacillus reuteri CRL1324 could be considered as a new biological agent to reduce infections caused by this micro-organism.
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
description AIMS: To assess the preventive effect of different intravaginal (i.va.) doses of Lactobacillus reuteri CRL1324 against vaginal colonization by Group B Streptococcus (GBS) in a murine experimental model. METHODS AND RESULTS: The major virulence factors of four vaginal GBS clinical isolates were determined to select the most virulent strain and set up a murine model of streptococcal vaginal colonization. Later, the effect of four and seven doses of 10(8) viable cells of Lact. reuteri CRL1324 i.va. administered, prior to the GBS challenge was studied. Seven doses of lactobacilli were able to significantly reduce the number of viable GBS cells, while four doses showed no preventive effect. Both doses reduced the leucocyte influx induced by GBS. Seven doses caused a slight increase in the Lact. reuteri CRL1324 vaginal colonization compared with four doses and reduced murine vaginal pH compared to control mice. CONCLUSIONS: Lactobacillus reuteri CRL1324 evidenced a preventive effect on GBS vaginal colonization in an experimental mouse model. SIGNIFICANCE AND IMPACTS OF THE STUDY: Maternal GBS colonization is one of the most important risk factors for developing disease in newborns. Lactobacillus reuteri CRL1324 could be considered as a new biological agent to reduce infections caused by this micro-organism.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28598
de Gregorio, Priscilla Romina; Juárez Tomás, María Silvina; Leccese Terraf, Maria Cecilia; Nader, Maria Elena Fatima; Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model; Wiley; Journal of Applied Microbiology; 118; 4; 29-1-2015; 1034-1047
1364-5072
1365-2672
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28598
identifier_str_mv de Gregorio, Priscilla Romina; Juárez Tomás, María Silvina; Leccese Terraf, Maria Cecilia; Nader, Maria Elena Fatima; Preventive effect of Lactobacillus reuteri CRL1324 on group B Streptococcus vaginal colonization in an experimental mouse model; Wiley; Journal of Applied Microbiology; 118; 4; 29-1-2015; 1034-1047
1364-5072
1365-2672
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12739
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12739/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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