Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice

Autores
de Gregorio, Priscila; Juárez Tomás, María Silvina; Salva, Maria Susana; Santos, Viviana; Álvarez, Susana; Nader, Maria Elena Fatima
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The effect of progesterone (P) and estradiol (E) on several organs of an experimental animal model was evaluated. 2-months-old female BALB/c mice were inoculated with (P) (day -5) or E (day -2) to induce and maintain the different stages of the estrous cycle. The samples taken on days 0, 2, 6 and 8 post-injection of hormones were: blood, vaginal fluid (VF), vagina, spleen and bone marrow (BM). Most of the animals remained in diestrous-metaestrous stages until day 6 post-P-injection or in proestrous-estrous stages until day 8 post-E-injection. The numbers of enterobacteria, staphylococci and lactic acid bacteria in VF were higher in estrogenized animals. In VF, myeloid population (Gr-1+) was dominant compared to T (CD3+) and B (B220+) lymphoid populations, determined by flow cytometry. On most of days, the Gr-1+ cells of VF were higher in estrogenized mice. The hormonal treatments did not produce significant differences in the myeloid and T cell populations of BM or spleen. Only in spleen, a significant increase of mature B cells (B220HighCD24Low) and a decrease of immature B cells (B220LowCD24High) were observed in P-inoculated mice compared with those E-inoculated. These results allow the selection of the most suitable experimental conditions to further evaluate the mechanisms of interaction between beneficial vaginal lactobacilli and the host in a murine experimental model.
Fil: de Gregorio, Priscila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Santos, Viviana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Álvarez, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
46th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Puerto Madryn
Argentina
Sociedad Argentina de Investigaciones Biológicas
Materia
Exogenous sexual hormones
Vaginal microbiota
Immune cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/256814
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/256814
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in micede Gregorio, PriscilaJuárez Tomás, María SilvinaSalva, Maria SusanaSantos, VivianaÁlvarez, SusanaNader, Maria Elena FatimaExogenous sexual hormonesVaginal microbiotaImmune cellshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The effect of progesterone (P) and estradiol (E) on several organs of an experimental animal model was evaluated. 2-months-old female BALB/c mice were inoculated with (P) (day -5) or E (day -2) to induce and maintain the different stages of the estrous cycle. The samples taken on days 0, 2, 6 and 8 post-injection of hormones were: blood, vaginal fluid (VF), vagina, spleen and bone marrow (BM). Most of the animals remained in diestrous-metaestrous stages until day 6 post-P-injection or in proestrous-estrous stages until day 8 post-E-injection. The numbers of enterobacteria, staphylococci and lactic acid bacteria in VF were higher in estrogenized animals. In VF, myeloid population (Gr-1+) was dominant compared to T (CD3+) and B (B220+) lymphoid populations, determined by flow cytometry. On most of days, the Gr-1+ cells of VF were higher in estrogenized mice. The hormonal treatments did not produce significant differences in the myeloid and T cell populations of BM or spleen. Only in spleen, a significant increase of mature B cells (B220HighCD24Low) and a decrease of immature B cells (B220LowCD24High) were observed in P-inoculated mice compared with those E-inoculated. These results allow the selection of the most suitable experimental conditions to further evaluate the mechanisms of interaction between beneficial vaginal lactobacilli and the host in a murine experimental model.Fil: de Gregorio, Priscila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Santos, Viviana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Álvarez, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina46th Annual Meeting Argentine Society for Biochemistry and Molecular BiologyPuerto MadrynArgentinaSociedad Argentina de Investigaciones BiológicasTech Science Press2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/256814Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice; 46th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Puerto Madryn; Argentina; 2010; 77-770327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/publicaciones/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:16:12Zoai:ri.conicet.gov.ar:11336/256814instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:16:12.919CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice
title Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice
spellingShingle Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice
de Gregorio, Priscila
Exogenous sexual hormones
Vaginal microbiota
Immune cells
title_short Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice
title_full Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice
title_fullStr Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice
title_full_unstemmed Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice
title_sort Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice
dc.creator.none.fl_str_mv de Gregorio, Priscila
Juárez Tomás, María Silvina
Salva, Maria Susana
Santos, Viviana
Álvarez, Susana
Nader, Maria Elena Fatima
author de Gregorio, Priscila
author_facet de Gregorio, Priscila
Juárez Tomás, María Silvina
Salva, Maria Susana
Santos, Viviana
Álvarez, Susana
Nader, Maria Elena Fatima
author_role author
author2 Juárez Tomás, María Silvina
Salva, Maria Susana
Santos, Viviana
Álvarez, Susana
Nader, Maria Elena Fatima
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Exogenous sexual hormones
Vaginal microbiota
Immune cells
topic Exogenous sexual hormones
Vaginal microbiota
Immune cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The effect of progesterone (P) and estradiol (E) on several organs of an experimental animal model was evaluated. 2-months-old female BALB/c mice were inoculated with (P) (day -5) or E (day -2) to induce and maintain the different stages of the estrous cycle. The samples taken on days 0, 2, 6 and 8 post-injection of hormones were: blood, vaginal fluid (VF), vagina, spleen and bone marrow (BM). Most of the animals remained in diestrous-metaestrous stages until day 6 post-P-injection or in proestrous-estrous stages until day 8 post-E-injection. The numbers of enterobacteria, staphylococci and lactic acid bacteria in VF were higher in estrogenized animals. In VF, myeloid population (Gr-1+) was dominant compared to T (CD3+) and B (B220+) lymphoid populations, determined by flow cytometry. On most of days, the Gr-1+ cells of VF were higher in estrogenized mice. The hormonal treatments did not produce significant differences in the myeloid and T cell populations of BM or spleen. Only in spleen, a significant increase of mature B cells (B220HighCD24Low) and a decrease of immature B cells (B220LowCD24High) were observed in P-inoculated mice compared with those E-inoculated. These results allow the selection of the most suitable experimental conditions to further evaluate the mechanisms of interaction between beneficial vaginal lactobacilli and the host in a murine experimental model.
Fil: de Gregorio, Priscila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Santos, Viviana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Álvarez, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
46th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Puerto Madryn
Argentina
Sociedad Argentina de Investigaciones Biológicas
description The effect of progesterone (P) and estradiol (E) on several organs of an experimental animal model was evaluated. 2-months-old female BALB/c mice were inoculated with (P) (day -5) or E (day -2) to induce and maintain the different stages of the estrous cycle. The samples taken on days 0, 2, 6 and 8 post-injection of hormones were: blood, vaginal fluid (VF), vagina, spleen and bone marrow (BM). Most of the animals remained in diestrous-metaestrous stages until day 6 post-P-injection or in proestrous-estrous stages until day 8 post-E-injection. The numbers of enterobacteria, staphylococci and lactic acid bacteria in VF were higher in estrogenized animals. In VF, myeloid population (Gr-1+) was dominant compared to T (CD3+) and B (B220+) lymphoid populations, determined by flow cytometry. On most of days, the Gr-1+ cells of VF were higher in estrogenized mice. The hormonal treatments did not produce significant differences in the myeloid and T cell populations of BM or spleen. Only in spleen, a significant increase of mature B cells (B220HighCD24Low) and a decrease of immature B cells (B220LowCD24High) were observed in P-inoculated mice compared with those E-inoculated. These results allow the selection of the most suitable experimental conditions to further evaluate the mechanisms of interaction between beneficial vaginal lactobacilli and the host in a murine experimental model.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/256814
Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice; 46th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Puerto Madryn; Argentina; 2010; 77-77
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/256814
identifier_str_mv Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice; 46th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Puerto Madryn; Argentina; 2010; 77-77
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/publicaciones/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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