Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk

Autores
Springer, Valeria Haydee; Lista, Adriana Guillermina
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A method to determine four antibiotics for veterinary use (ciprofloxacin, enrofloxacin, florfenicol, and chloramphenicol) of different families (fluoroquinolones and amphenicols) in bovine milk was developed. The determination of the analytes was carried out using micellar electrokinetic capillary chromatography (MEKC) with a common sodium borate-SDS buffer solution containing single-walled carbon nanotubes (SWCNTs). In this way, a great improvement in the electrophoretic resolution and the separation efficiency was achieved compared to MEKC. An online reverse electrode polarity-stacking mode (REPSM) was carried out to enhance sensitivity. This step was performed in only 2 min and it allowed a stacked percentage of 103. That means that all the amount of injected analytes is effectively stacked. When this stacking procedure was combined with an off-line preconcentration step, based on SPE, analytes could be detected in lower concentration than the established maximum residue limits (MRLs). The LODs for the four compounds were between 6.8 and 13.8 μg L -1 and the RSD values were between 1.1% and 6.6%. The whole method was applied to spiked real samples with acceptable precision and satisfactory recoveries. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Fil: Springer, Valeria Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lista, Adriana Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Materia
Antibiotics
Carbon Nanotubes
Micellar Electrokinetic Chromatography
Sample Stacking
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64035
Acceder
id CONICETDig_1a00593a08a8c28bfa52c7ad272718db
oai_identifier_str oai:ri.conicet.gov.ar:11336/64035
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milkSpringer, Valeria HaydeeLista, Adriana GuillerminaAntibioticsCarbon NanotubesMicellar Electrokinetic ChromatographySample Stackinghttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A method to determine four antibiotics for veterinary use (ciprofloxacin, enrofloxacin, florfenicol, and chloramphenicol) of different families (fluoroquinolones and amphenicols) in bovine milk was developed. The determination of the analytes was carried out using micellar electrokinetic capillary chromatography (MEKC) with a common sodium borate-SDS buffer solution containing single-walled carbon nanotubes (SWCNTs). In this way, a great improvement in the electrophoretic resolution and the separation efficiency was achieved compared to MEKC. An online reverse electrode polarity-stacking mode (REPSM) was carried out to enhance sensitivity. This step was performed in only 2 min and it allowed a stacked percentage of 103. That means that all the amount of injected analytes is effectively stacked. When this stacking procedure was combined with an off-line preconcentration step, based on SPE, analytes could be detected in lower concentration than the established maximum residue limits (MRLs). The LODs for the four compounds were between 6.8 and 13.8 μg L -1 and the RSD values were between 1.1% and 6.6%. The whole method was applied to spiked real samples with acceptable precision and satisfactory recoveries. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Fil: Springer, Valeria Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Lista, Adriana Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaWiley VCH Verlag2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64035Springer, Valeria Haydee; Lista, Adriana Guillermina; Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk; Wiley VCH Verlag; Electrophoresis; 33; 13; 7-2012; 2049-20550173-0835CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201100713info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201100713info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:20:05Zoai:ri.conicet.gov.ar:11336/64035instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:20:05.554CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk
title Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk
spellingShingle Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk
Springer, Valeria Haydee
Antibiotics
Carbon Nanotubes
Micellar Electrokinetic Chromatography
Sample Stacking
title_short Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk
title_full Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk
title_fullStr Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk
title_full_unstemmed Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk
title_sort Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk
dc.creator.none.fl_str_mv Springer, Valeria Haydee
Lista, Adriana Guillermina
author Springer, Valeria Haydee
author_facet Springer, Valeria Haydee
Lista, Adriana Guillermina
author_role author
author2 Lista, Adriana Guillermina
author2_role author
dc.subject.none.fl_str_mv Antibiotics
Carbon Nanotubes
Micellar Electrokinetic Chromatography
Sample Stacking
topic Antibiotics
Carbon Nanotubes
Micellar Electrokinetic Chromatography
Sample Stacking
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A method to determine four antibiotics for veterinary use (ciprofloxacin, enrofloxacin, florfenicol, and chloramphenicol) of different families (fluoroquinolones and amphenicols) in bovine milk was developed. The determination of the analytes was carried out using micellar electrokinetic capillary chromatography (MEKC) with a common sodium borate-SDS buffer solution containing single-walled carbon nanotubes (SWCNTs). In this way, a great improvement in the electrophoretic resolution and the separation efficiency was achieved compared to MEKC. An online reverse electrode polarity-stacking mode (REPSM) was carried out to enhance sensitivity. This step was performed in only 2 min and it allowed a stacked percentage of 103. That means that all the amount of injected analytes is effectively stacked. When this stacking procedure was combined with an off-line preconcentration step, based on SPE, analytes could be detected in lower concentration than the established maximum residue limits (MRLs). The LODs for the four compounds were between 6.8 and 13.8 μg L -1 and the RSD values were between 1.1% and 6.6%. The whole method was applied to spiked real samples with acceptable precision and satisfactory recoveries. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Fil: Springer, Valeria Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lista, Adriana Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
description A method to determine four antibiotics for veterinary use (ciprofloxacin, enrofloxacin, florfenicol, and chloramphenicol) of different families (fluoroquinolones and amphenicols) in bovine milk was developed. The determination of the analytes was carried out using micellar electrokinetic capillary chromatography (MEKC) with a common sodium borate-SDS buffer solution containing single-walled carbon nanotubes (SWCNTs). In this way, a great improvement in the electrophoretic resolution and the separation efficiency was achieved compared to MEKC. An online reverse electrode polarity-stacking mode (REPSM) was carried out to enhance sensitivity. This step was performed in only 2 min and it allowed a stacked percentage of 103. That means that all the amount of injected analytes is effectively stacked. When this stacking procedure was combined with an off-line preconcentration step, based on SPE, analytes could be detected in lower concentration than the established maximum residue limits (MRLs). The LODs for the four compounds were between 6.8 and 13.8 μg L -1 and the RSD values were between 1.1% and 6.6%. The whole method was applied to spiked real samples with acceptable precision and satisfactory recoveries. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64035
Springer, Valeria Haydee; Lista, Adriana Guillermina; Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk; Wiley VCH Verlag; Electrophoresis; 33; 13; 7-2012; 2049-2055
0173-0835
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64035
identifier_str_mv Springer, Valeria Haydee; Lista, Adriana Guillermina; Micellar nanotubes dispersed electrokinetic chromatography for the simultaneous determination of antibiotics in bovine milk; Wiley VCH Verlag; Electrophoresis; 33; 13; 7-2012; 2049-2055
0173-0835
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201100713
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201100713
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782645210447872
score 12.982451

Publicaciones similares