New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations

Autores
Contin, Mario Daniel; Buontempo, Fabián; García Becerra, Cristian Ezequiel; Dobrecky, Cecilia Beatriz; Lucangioli, Silvia Edith; Tripodi, Valeria Paula
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A novel, simple and reliable method based on micellar electrokinetic chromatography with ultraviolet detection was developed to analyze idebenone in a pediatric formulation. Idebenone is a synthetic short chain benzoquinone that acts as an electron carrier in the mitochondrial electron transport chain facilitating the production of adenosine triphosphate. It can be found in two different redox states that differ in their physiological properties. Idebenone has been investigated as a treatment in several neurological disorders like Friedreich's ataxia, Leber's hereditary optic neuropathy, mitochondrial encephalomyopathies and senile dementia. Accordingly, a micellar electrokinetic chromatography was employed to discriminate both redox forms. The final optimized system was validated in terms of selectivity, linearity (r2 0.992), limit of detection (0.5 μg/mL), limit of quantification (1.8 μg/mL), intra- and inter-day precision (RSD ≤ 2) and accuracy in terms of recovery studies (99.3-100.5%). Robustness was studied following a Plackett-Burman design. Finally, the validated system was applied to the analysis of idebenone in a pediatric formulation.
Fil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Buontempo, Fabián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: García Becerra, Cristian Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Dobrecky, Cecilia Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
IDEBENONE
MICELLAR ELECTROKINETIC CHROMATOGRAHY
FORMULATION
PEDIATRIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212523
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/212523
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric FormulationsContin, Mario DanielBuontempo, FabiánGarcía Becerra, Cristian EzequielDobrecky, Cecilia BeatrizLucangioli, Silvia EdithTripodi, Valeria PaulaIDEBENONEMICELLAR ELECTROKINETIC CHROMATOGRAHYFORMULATIONPEDIATRIChttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3A novel, simple and reliable method based on micellar electrokinetic chromatography with ultraviolet detection was developed to analyze idebenone in a pediatric formulation. Idebenone is a synthetic short chain benzoquinone that acts as an electron carrier in the mitochondrial electron transport chain facilitating the production of adenosine triphosphate. It can be found in two different redox states that differ in their physiological properties. Idebenone has been investigated as a treatment in several neurological disorders like Friedreich's ataxia, Leber's hereditary optic neuropathy, mitochondrial encephalomyopathies and senile dementia. Accordingly, a micellar electrokinetic chromatography was employed to discriminate both redox forms. The final optimized system was validated in terms of selectivity, linearity (r2 0.992), limit of detection (0.5 μg/mL), limit of quantification (1.8 μg/mL), intra- and inter-day precision (RSD ≤ 2) and accuracy in terms of recovery studies (99.3-100.5%). Robustness was studied following a Plackett-Burman design. Finally, the validated system was applied to the analysis of idebenone in a pediatric formulation.Fil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Buontempo, Fabián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: García Becerra, Cristian Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Dobrecky, Cecilia Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPreston Publ Inc2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212523Contin, Mario Daniel; Buontempo, Fabián; García Becerra, Cristian Ezequiel; Dobrecky, Cecilia Beatriz; Lucangioli, Silvia Edith; et al.; New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations; Preston Publ Inc; Journal Of Chromatographic Science; 55; 3; 11-2017; 351-3570021-9665CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/chromsci/bmw172info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:19:41Zoai:ri.conicet.gov.ar:11336/212523instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:19:41.591CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations
title New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations
spellingShingle New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations
Contin, Mario Daniel
IDEBENONE
MICELLAR ELECTROKINETIC CHROMATOGRAHY
FORMULATION
PEDIATRIC
title_short New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations
title_full New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations
title_fullStr New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations
title_full_unstemmed New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations
title_sort New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations
dc.creator.none.fl_str_mv Contin, Mario Daniel
Buontempo, Fabián
García Becerra, Cristian Ezequiel
Dobrecky, Cecilia Beatriz
Lucangioli, Silvia Edith
Tripodi, Valeria Paula
author Contin, Mario Daniel
author_facet Contin, Mario Daniel
Buontempo, Fabián
García Becerra, Cristian Ezequiel
Dobrecky, Cecilia Beatriz
Lucangioli, Silvia Edith
Tripodi, Valeria Paula
author_role author
author2 Buontempo, Fabián
García Becerra, Cristian Ezequiel
Dobrecky, Cecilia Beatriz
Lucangioli, Silvia Edith
Tripodi, Valeria Paula
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv IDEBENONE
MICELLAR ELECTROKINETIC CHROMATOGRAHY
FORMULATION
PEDIATRIC
topic IDEBENONE
MICELLAR ELECTROKINETIC CHROMATOGRAHY
FORMULATION
PEDIATRIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A novel, simple and reliable method based on micellar electrokinetic chromatography with ultraviolet detection was developed to analyze idebenone in a pediatric formulation. Idebenone is a synthetic short chain benzoquinone that acts as an electron carrier in the mitochondrial electron transport chain facilitating the production of adenosine triphosphate. It can be found in two different redox states that differ in their physiological properties. Idebenone has been investigated as a treatment in several neurological disorders like Friedreich's ataxia, Leber's hereditary optic neuropathy, mitochondrial encephalomyopathies and senile dementia. Accordingly, a micellar electrokinetic chromatography was employed to discriminate both redox forms. The final optimized system was validated in terms of selectivity, linearity (r2 0.992), limit of detection (0.5 μg/mL), limit of quantification (1.8 μg/mL), intra- and inter-day precision (RSD ≤ 2) and accuracy in terms of recovery studies (99.3-100.5%). Robustness was studied following a Plackett-Burman design. Finally, the validated system was applied to the analysis of idebenone in a pediatric formulation.
Fil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Buontempo, Fabián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: García Becerra, Cristian Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Dobrecky, Cecilia Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description A novel, simple and reliable method based on micellar electrokinetic chromatography with ultraviolet detection was developed to analyze idebenone in a pediatric formulation. Idebenone is a synthetic short chain benzoquinone that acts as an electron carrier in the mitochondrial electron transport chain facilitating the production of adenosine triphosphate. It can be found in two different redox states that differ in their physiological properties. Idebenone has been investigated as a treatment in several neurological disorders like Friedreich's ataxia, Leber's hereditary optic neuropathy, mitochondrial encephalomyopathies and senile dementia. Accordingly, a micellar electrokinetic chromatography was employed to discriminate both redox forms. The final optimized system was validated in terms of selectivity, linearity (r2 0.992), limit of detection (0.5 μg/mL), limit of quantification (1.8 μg/mL), intra- and inter-day precision (RSD ≤ 2) and accuracy in terms of recovery studies (99.3-100.5%). Robustness was studied following a Plackett-Burman design. Finally, the validated system was applied to the analysis of idebenone in a pediatric formulation.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212523
Contin, Mario Daniel; Buontempo, Fabián; García Becerra, Cristian Ezequiel; Dobrecky, Cecilia Beatriz; Lucangioli, Silvia Edith; et al.; New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations; Preston Publ Inc; Journal Of Chromatographic Science; 55; 3; 11-2017; 351-357
0021-9665
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212523
identifier_str_mv Contin, Mario Daniel; Buontempo, Fabián; García Becerra, Cristian Ezequiel; Dobrecky, Cecilia Beatriz; Lucangioli, Silvia Edith; et al.; New Micellar Electrokinetic Chromatographic Method for Analyzing Idebenone in Pediatric Formulations; Preston Publ Inc; Journal Of Chromatographic Science; 55; 3; 11-2017; 351-357
0021-9665
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/chromsci/bmw172
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Preston Publ Inc
publisher.none.fl_str_mv Preston Publ Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781677032964096
score 12.982451

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