Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil

Autores
Stege, Patricia Wanda; Messina, Germán Alejandro; Sombra, Lorena Luján; Bianchi, Guillermo; Olsina, Roberto Antonio
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Inorganic phosphorous (P) is an essential plant nutrient that must be added to most soils to maintain plant growth and sustain crop yields. Phosphatase enzymes play a major role in the processes of mineralization of organic P substrates. In the present study we propose a precise and accurate method to determine the alkaline and acid phosphatase activity in soil using adenosine monophosphate (AMP) as a substrate. The adenosine released by the enzymatic reaction was quantified by micellar electrokinetic chromatography (MEKC) method. The background electrolyte (BGE) consisted in 20 mM phosphate buffer, 10 mM sodium dodecyl sulphate (SDS) and 10% acetonitrile (ACN). The LOD and LOQ for adenosine were 2.27 × 10-4 and 6.4 × 10-4 mg mL-1, respectively. This sensitive method allowed the use of natural compound as enzymatic substrate, which is commonly found in soil. Moreover, we improve the detection method by diminishing the amount of waste and using a harmless substrate.
Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Bianchi, Guillermo. Universidad de Los Andes; Venezuela
Fil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
ACID PHOSPHATASE
ADENOSINE MONOPHOSPHATE
ALKALINE PHOSPHATASE
ENZYME ACTIVITY
MICELLAR ELECTROKINETIC CHROMATOGRAPHY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93228
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/93228
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soilStege, Patricia WandaMessina, Germán AlejandroSombra, Lorena LujánBianchi, GuillermoOlsina, Roberto AntonioACID PHOSPHATASEADENOSINE MONOPHOSPHATEALKALINE PHOSPHATASEENZYME ACTIVITYMICELLAR ELECTROKINETIC CHROMATOGRAPHYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Inorganic phosphorous (P) is an essential plant nutrient that must be added to most soils to maintain plant growth and sustain crop yields. Phosphatase enzymes play a major role in the processes of mineralization of organic P substrates. In the present study we propose a precise and accurate method to determine the alkaline and acid phosphatase activity in soil using adenosine monophosphate (AMP) as a substrate. The adenosine released by the enzymatic reaction was quantified by micellar electrokinetic chromatography (MEKC) method. The background electrolyte (BGE) consisted in 20 mM phosphate buffer, 10 mM sodium dodecyl sulphate (SDS) and 10% acetonitrile (ACN). The LOD and LOQ for adenosine were 2.27 × 10-4 and 6.4 × 10-4 mg mL-1, respectively. This sensitive method allowed the use of natural compound as enzymatic substrate, which is commonly found in soil. Moreover, we improve the detection method by diminishing the amount of waste and using a harmless substrate.Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Bianchi, Guillermo. Universidad de Los Andes; VenezuelaFil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science Sa2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93228Stege, Patricia Wanda; Messina, Germán Alejandro; Sombra, Lorena Luján; Bianchi, Guillermo; Olsina, Roberto Antonio; Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil; Elsevier Science Sa; Biochemical Engineering Journal; 46; 2; 10-2009; 121-1251369-703XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bej.2009.04.018info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1369703X09001387info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:31:36Zoai:ri.conicet.gov.ar:11336/93228instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:31:37.153CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
title Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
spellingShingle Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
Stege, Patricia Wanda
ACID PHOSPHATASE
ADENOSINE MONOPHOSPHATE
ALKALINE PHOSPHATASE
ENZYME ACTIVITY
MICELLAR ELECTROKINETIC CHROMATOGRAPHY
title_short Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
title_full Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
title_fullStr Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
title_full_unstemmed Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
title_sort Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
dc.creator.none.fl_str_mv Stege, Patricia Wanda
Messina, Germán Alejandro
Sombra, Lorena Luján
Bianchi, Guillermo
Olsina, Roberto Antonio
author Stege, Patricia Wanda
author_facet Stege, Patricia Wanda
Messina, Germán Alejandro
Sombra, Lorena Luján
Bianchi, Guillermo
Olsina, Roberto Antonio
author_role author
author2 Messina, Germán Alejandro
Sombra, Lorena Luján
Bianchi, Guillermo
Olsina, Roberto Antonio
author2_role author
author
author
author
dc.subject.none.fl_str_mv ACID PHOSPHATASE
ADENOSINE MONOPHOSPHATE
ALKALINE PHOSPHATASE
ENZYME ACTIVITY
MICELLAR ELECTROKINETIC CHROMATOGRAPHY
topic ACID PHOSPHATASE
ADENOSINE MONOPHOSPHATE
ALKALINE PHOSPHATASE
ENZYME ACTIVITY
MICELLAR ELECTROKINETIC CHROMATOGRAPHY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Inorganic phosphorous (P) is an essential plant nutrient that must be added to most soils to maintain plant growth and sustain crop yields. Phosphatase enzymes play a major role in the processes of mineralization of organic P substrates. In the present study we propose a precise and accurate method to determine the alkaline and acid phosphatase activity in soil using adenosine monophosphate (AMP) as a substrate. The adenosine released by the enzymatic reaction was quantified by micellar electrokinetic chromatography (MEKC) method. The background electrolyte (BGE) consisted in 20 mM phosphate buffer, 10 mM sodium dodecyl sulphate (SDS) and 10% acetonitrile (ACN). The LOD and LOQ for adenosine were 2.27 × 10-4 and 6.4 × 10-4 mg mL-1, respectively. This sensitive method allowed the use of natural compound as enzymatic substrate, which is commonly found in soil. Moreover, we improve the detection method by diminishing the amount of waste and using a harmless substrate.
Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Bianchi, Guillermo. Universidad de Los Andes; Venezuela
Fil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description Inorganic phosphorous (P) is an essential plant nutrient that must be added to most soils to maintain plant growth and sustain crop yields. Phosphatase enzymes play a major role in the processes of mineralization of organic P substrates. In the present study we propose a precise and accurate method to determine the alkaline and acid phosphatase activity in soil using adenosine monophosphate (AMP) as a substrate. The adenosine released by the enzymatic reaction was quantified by micellar electrokinetic chromatography (MEKC) method. The background electrolyte (BGE) consisted in 20 mM phosphate buffer, 10 mM sodium dodecyl sulphate (SDS) and 10% acetonitrile (ACN). The LOD and LOQ for adenosine were 2.27 × 10-4 and 6.4 × 10-4 mg mL-1, respectively. This sensitive method allowed the use of natural compound as enzymatic substrate, which is commonly found in soil. Moreover, we improve the detection method by diminishing the amount of waste and using a harmless substrate.
publishDate 2009
dc.date.none.fl_str_mv 2009-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93228
Stege, Patricia Wanda; Messina, Germán Alejandro; Sombra, Lorena Luján; Bianchi, Guillermo; Olsina, Roberto Antonio; Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil; Elsevier Science Sa; Biochemical Engineering Journal; 46; 2; 10-2009; 121-125
1369-703X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93228
identifier_str_mv Stege, Patricia Wanda; Messina, Germán Alejandro; Sombra, Lorena Luján; Bianchi, Guillermo; Olsina, Roberto Antonio; Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil; Elsevier Science Sa; Biochemical Engineering Journal; 46; 2; 10-2009; 121-125
1369-703X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bej.2009.04.018
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1369703X09001387
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781921397309440
score 12.982451

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