Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
- Autores
- Stege, Patricia Wanda; Messina, Germán Alejandro; Sombra, Lorena Luján; Bianchi, Guillermo; Olsina, Roberto Antonio
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Inorganic phosphorous (P) is an essential plant nutrient that must be added to most soils to maintain plant growth and sustain crop yields. Phosphatase enzymes play a major role in the processes of mineralization of organic P substrates. In the present study we propose a precise and accurate method to determine the alkaline and acid phosphatase activity in soil using adenosine monophosphate (AMP) as a substrate. The adenosine released by the enzymatic reaction was quantified by micellar electrokinetic chromatography (MEKC) method. The background electrolyte (BGE) consisted in 20 mM phosphate buffer, 10 mM sodium dodecyl sulphate (SDS) and 10% acetonitrile (ACN). The LOD and LOQ for adenosine were 2.27 × 10-4 and 6.4 × 10-4 mg mL-1, respectively. This sensitive method allowed the use of natural compound as enzymatic substrate, which is commonly found in soil. Moreover, we improve the detection method by diminishing the amount of waste and using a harmless substrate.
Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Bianchi, Guillermo. Universidad de Los Andes; Venezuela
Fil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina - Materia
-
ACID PHOSPHATASE
ADENOSINE MONOPHOSPHATE
ALKALINE PHOSPHATASE
ENZYME ACTIVITY
MICELLAR ELECTROKINETIC CHROMATOGRAPHY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/93228
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/93228 |
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3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soilStege, Patricia WandaMessina, Germán AlejandroSombra, Lorena LujánBianchi, GuillermoOlsina, Roberto AntonioACID PHOSPHATASEADENOSINE MONOPHOSPHATEALKALINE PHOSPHATASEENZYME ACTIVITYMICELLAR ELECTROKINETIC CHROMATOGRAPHYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Inorganic phosphorous (P) is an essential plant nutrient that must be added to most soils to maintain plant growth and sustain crop yields. Phosphatase enzymes play a major role in the processes of mineralization of organic P substrates. In the present study we propose a precise and accurate method to determine the alkaline and acid phosphatase activity in soil using adenosine monophosphate (AMP) as a substrate. The adenosine released by the enzymatic reaction was quantified by micellar electrokinetic chromatography (MEKC) method. The background electrolyte (BGE) consisted in 20 mM phosphate buffer, 10 mM sodium dodecyl sulphate (SDS) and 10% acetonitrile (ACN). The LOD and LOQ for adenosine were 2.27 × 10-4 and 6.4 × 10-4 mg mL-1, respectively. This sensitive method allowed the use of natural compound as enzymatic substrate, which is commonly found in soil. Moreover, we improve the detection method by diminishing the amount of waste and using a harmless substrate.Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Bianchi, Guillermo. Universidad de Los Andes; VenezuelaFil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science Sa2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93228Stege, Patricia Wanda; Messina, Germán Alejandro; Sombra, Lorena Luján; Bianchi, Guillermo; Olsina, Roberto Antonio; Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil; Elsevier Science Sa; Biochemical Engineering Journal; 46; 2; 10-2009; 121-1251369-703XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bej.2009.04.018info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1369703X09001387info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:09Zoai:ri.conicet.gov.ar:11336/93228instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:09.563CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil |
title |
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil |
spellingShingle |
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil Stege, Patricia Wanda ACID PHOSPHATASE ADENOSINE MONOPHOSPHATE ALKALINE PHOSPHATASE ENZYME ACTIVITY MICELLAR ELECTROKINETIC CHROMATOGRAPHY |
title_short |
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil |
title_full |
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil |
title_fullStr |
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil |
title_full_unstemmed |
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil |
title_sort |
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil |
dc.creator.none.fl_str_mv |
Stege, Patricia Wanda Messina, Germán Alejandro Sombra, Lorena Luján Bianchi, Guillermo Olsina, Roberto Antonio |
author |
Stege, Patricia Wanda |
author_facet |
Stege, Patricia Wanda Messina, Germán Alejandro Sombra, Lorena Luján Bianchi, Guillermo Olsina, Roberto Antonio |
author_role |
author |
author2 |
Messina, Germán Alejandro Sombra, Lorena Luján Bianchi, Guillermo Olsina, Roberto Antonio |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
ACID PHOSPHATASE ADENOSINE MONOPHOSPHATE ALKALINE PHOSPHATASE ENZYME ACTIVITY MICELLAR ELECTROKINETIC CHROMATOGRAPHY |
topic |
ACID PHOSPHATASE ADENOSINE MONOPHOSPHATE ALKALINE PHOSPHATASE ENZYME ACTIVITY MICELLAR ELECTROKINETIC CHROMATOGRAPHY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Inorganic phosphorous (P) is an essential plant nutrient that must be added to most soils to maintain plant growth and sustain crop yields. Phosphatase enzymes play a major role in the processes of mineralization of organic P substrates. In the present study we propose a precise and accurate method to determine the alkaline and acid phosphatase activity in soil using adenosine monophosphate (AMP) as a substrate. The adenosine released by the enzymatic reaction was quantified by micellar electrokinetic chromatography (MEKC) method. The background electrolyte (BGE) consisted in 20 mM phosphate buffer, 10 mM sodium dodecyl sulphate (SDS) and 10% acetonitrile (ACN). The LOD and LOQ for adenosine were 2.27 × 10-4 and 6.4 × 10-4 mg mL-1, respectively. This sensitive method allowed the use of natural compound as enzymatic substrate, which is commonly found in soil. Moreover, we improve the detection method by diminishing the amount of waste and using a harmless substrate. Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Bianchi, Guillermo. Universidad de Los Andes; Venezuela Fil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina |
description |
Inorganic phosphorous (P) is an essential plant nutrient that must be added to most soils to maintain plant growth and sustain crop yields. Phosphatase enzymes play a major role in the processes of mineralization of organic P substrates. In the present study we propose a precise and accurate method to determine the alkaline and acid phosphatase activity in soil using adenosine monophosphate (AMP) as a substrate. The adenosine released by the enzymatic reaction was quantified by micellar electrokinetic chromatography (MEKC) method. The background electrolyte (BGE) consisted in 20 mM phosphate buffer, 10 mM sodium dodecyl sulphate (SDS) and 10% acetonitrile (ACN). The LOD and LOQ for adenosine were 2.27 × 10-4 and 6.4 × 10-4 mg mL-1, respectively. This sensitive method allowed the use of natural compound as enzymatic substrate, which is commonly found in soil. Moreover, we improve the detection method by diminishing the amount of waste and using a harmless substrate. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/93228 Stege, Patricia Wanda; Messina, Germán Alejandro; Sombra, Lorena Luján; Bianchi, Guillermo; Olsina, Roberto Antonio; Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil; Elsevier Science Sa; Biochemical Engineering Journal; 46; 2; 10-2009; 121-125 1369-703X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/93228 |
identifier_str_mv |
Stege, Patricia Wanda; Messina, Germán Alejandro; Sombra, Lorena Luján; Bianchi, Guillermo; Olsina, Roberto Antonio; Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil; Elsevier Science Sa; Biochemical Engineering Journal; 46; 2; 10-2009; 121-125 1369-703X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bej.2009.04.018 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1369703X09001387 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science Sa |
publisher.none.fl_str_mv |
Elsevier Science Sa |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613822500831232 |
score |
13.070432 |