Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis

Autores
Flor, Sabrina Andrea; Lucangioli, Silvia Edith; Contin, Mario Daniel; Tripodi, Valeria Paula
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new CE system based on the use of polymeric-mixed micelles (cholic acid, SDS and the poloxamine Tetronic® 1107) was developed for the simultaneous determination of nine steroids in human urine. This method allows the baseline separation and quantitation of cortisol, androstenedione, estriol, dehydroepiandrosterone sulfate, testosterone, dehydroepiandrosterone, estrone, progesterone and estradiol in less than 25 min showing to be sensitive enough to detect low concentrations of these steroids in urine samples (5–45 ng/mL). The optimized electrophoretic conditions were performed using a 50 cm×75 μm capillary, 18 kV, 25°C, with 44 mM cholic acid, 10 mM SDS, 0.05% w/v tetronic® 1107, 2.5% v/v methanol, 2.5% v/v tetrahydrofuran in 5 mM borate – 5 mM phosphate buffer (pH=8.0) as a background electrolyte and a dual 210/254 UV-detection. The method can simultaneously determine 0.1–120 μg/mL, which corresponds to 5–6000 ng/mL of steroids in 2 mL urine. The recoveries ranged between 82.4 and 101.5%. Due to its simplicity, speed, accuracy and reliability, the proposed method could be a potential alternative to the traditional methodologies used with clinical purposes.
Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Contin, Mario Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Tripodi, Valeria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Materia
Capillary Electrophoretic
Micelar Electrokinetic Capillary Chromatography
Polymeric Micelle
Steroids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14142
Acceder
id CONICETDig_012ffe9576c1c6741c73a08933258b88
oai_identifier_str oai:ri.conicet.gov.ar:11336/14142
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresisFlor, Sabrina AndreaLucangioli, Silvia EdithContin, Mario DanielTripodi, Valeria PaulaCapillary ElectrophoreticMicelar Electrokinetic Capillary ChromatographyPolymeric MicelleSteroidshttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3A new CE system based on the use of polymeric-mixed micelles (cholic acid, SDS and the poloxamine Tetronic® 1107) was developed for the simultaneous determination of nine steroids in human urine. This method allows the baseline separation and quantitation of cortisol, androstenedione, estriol, dehydroepiandrosterone sulfate, testosterone, dehydroepiandrosterone, estrone, progesterone and estradiol in less than 25 min showing to be sensitive enough to detect low concentrations of these steroids in urine samples (5–45 ng/mL). The optimized electrophoretic conditions were performed using a 50 cm×75 μm capillary, 18 kV, 25°C, with 44 mM cholic acid, 10 mM SDS, 0.05% w/v tetronic® 1107, 2.5% v/v methanol, 2.5% v/v tetrahydrofuran in 5 mM borate – 5 mM phosphate buffer (pH=8.0) as a background electrolyte and a dual 210/254 UV-detection. The method can simultaneously determine 0.1–120 μg/mL, which corresponds to 5–6000 ng/mL of steroids in 2 mL urine. The recoveries ranged between 82.4 and 101.5%. Due to its simplicity, speed, accuracy and reliability, the proposed method could be a potential alternative to the traditional methodologies used with clinical purposes.Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; ArgentinaFil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Contin, Mario Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; ArgentinaFil: Tripodi, Valeria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; ArgentinaWiley VCH Verlag2010-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14142Flor, Sabrina Andrea; Lucangioli, Silvia Edith; Contin, Mario Daniel; Tripodi, Valeria Paula; Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis; Wiley VCH Verlag; Electrophoresis; 31; 19; 10-2010; 3305-33130173-0835enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201000096/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201000096info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:50:52Zoai:ri.conicet.gov.ar:11336/14142instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:50:52.453CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis
title Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis
spellingShingle Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis
Flor, Sabrina Andrea
Capillary Electrophoretic
Micelar Electrokinetic Capillary Chromatography
Polymeric Micelle
Steroids
title_short Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis
title_full Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis
title_fullStr Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis
title_full_unstemmed Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis
title_sort Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis
dc.creator.none.fl_str_mv Flor, Sabrina Andrea
Lucangioli, Silvia Edith
Contin, Mario Daniel
Tripodi, Valeria Paula
author Flor, Sabrina Andrea
author_facet Flor, Sabrina Andrea
Lucangioli, Silvia Edith
Contin, Mario Daniel
Tripodi, Valeria Paula
author_role author
author2 Lucangioli, Silvia Edith
Contin, Mario Daniel
Tripodi, Valeria Paula
author2_role author
author
author
dc.subject.none.fl_str_mv Capillary Electrophoretic
Micelar Electrokinetic Capillary Chromatography
Polymeric Micelle
Steroids
topic Capillary Electrophoretic
Micelar Electrokinetic Capillary Chromatography
Polymeric Micelle
Steroids
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A new CE system based on the use of polymeric-mixed micelles (cholic acid, SDS and the poloxamine Tetronic® 1107) was developed for the simultaneous determination of nine steroids in human urine. This method allows the baseline separation and quantitation of cortisol, androstenedione, estriol, dehydroepiandrosterone sulfate, testosterone, dehydroepiandrosterone, estrone, progesterone and estradiol in less than 25 min showing to be sensitive enough to detect low concentrations of these steroids in urine samples (5–45 ng/mL). The optimized electrophoretic conditions were performed using a 50 cm×75 μm capillary, 18 kV, 25°C, with 44 mM cholic acid, 10 mM SDS, 0.05% w/v tetronic® 1107, 2.5% v/v methanol, 2.5% v/v tetrahydrofuran in 5 mM borate – 5 mM phosphate buffer (pH=8.0) as a background electrolyte and a dual 210/254 UV-detection. The method can simultaneously determine 0.1–120 μg/mL, which corresponds to 5–6000 ng/mL of steroids in 2 mL urine. The recoveries ranged between 82.4 and 101.5%. Due to its simplicity, speed, accuracy and reliability, the proposed method could be a potential alternative to the traditional methodologies used with clinical purposes.
Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Contin, Mario Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Tripodi, Valeria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
description A new CE system based on the use of polymeric-mixed micelles (cholic acid, SDS and the poloxamine Tetronic® 1107) was developed for the simultaneous determination of nine steroids in human urine. This method allows the baseline separation and quantitation of cortisol, androstenedione, estriol, dehydroepiandrosterone sulfate, testosterone, dehydroepiandrosterone, estrone, progesterone and estradiol in less than 25 min showing to be sensitive enough to detect low concentrations of these steroids in urine samples (5–45 ng/mL). The optimized electrophoretic conditions were performed using a 50 cm×75 μm capillary, 18 kV, 25°C, with 44 mM cholic acid, 10 mM SDS, 0.05% w/v tetronic® 1107, 2.5% v/v methanol, 2.5% v/v tetrahydrofuran in 5 mM borate – 5 mM phosphate buffer (pH=8.0) as a background electrolyte and a dual 210/254 UV-detection. The method can simultaneously determine 0.1–120 μg/mL, which corresponds to 5–6000 ng/mL of steroids in 2 mL urine. The recoveries ranged between 82.4 and 101.5%. Due to its simplicity, speed, accuracy and reliability, the proposed method could be a potential alternative to the traditional methodologies used with clinical purposes.
publishDate 2010
dc.date.none.fl_str_mv 2010-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14142
Flor, Sabrina Andrea; Lucangioli, Silvia Edith; Contin, Mario Daniel; Tripodi, Valeria Paula; Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis; Wiley VCH Verlag; Electrophoresis; 31; 19; 10-2010; 3305-3313
0173-0835
url http://hdl.handle.net/11336/14142
identifier_str_mv Flor, Sabrina Andrea; Lucangioli, Silvia Edith; Contin, Mario Daniel; Tripodi, Valeria Paula; Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis; Wiley VCH Verlag; Electrophoresis; 31; 19; 10-2010; 3305-3313
0173-0835
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201000096/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201000096
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083034081656832
score 13.22299

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