Membrane composition of jetted lipid vesicles: A Raman spectroscopy study

Autores
Kirchner, Silke R.; Ohlinger, Alexander; Pfeiffer, Tom; Urban, Alexander S.; Stefani, Fernando Daniel; Deak, Andras; Lutich, Andrey A.; Feldmann, Jochen
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microfluidic jetting is a promising method to produce giant unilamellar phospholipid vesicles for mimicking living cells in biomedical studies. We have investigated the chemical composition of membranes of vesicles prepared using this approach by means of Raman scattering spectroscopy. The membranes of all jetted vesicles are found to contain residuals of the organic solvent decane used in the preparation of the initial planar membrane. The decane inclusions are randomly distributed over the vesicle surface area and vary in thickness from a few to several tens of nanometers. Our findings point out that the membrane properties of jetted vesicles may differ considerably from those of vesicles prepared by other methods and from those of living cells.
Fil: Kirchner, Silke R.. Ludwig Maximilians Universitat; Alemania
Fil: Ohlinger, Alexander. Ludwig Maximilians Universitat; Alemania
Fil: Pfeiffer, Tom. Ludwig Maximilians Universitat; Alemania
Fil: Urban, Alexander S.. Ludwig Maximilians Universitat; Alemania
Fil: Stefani, Fernando Daniel. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Deak, Andras. Ludwig Maximilians Universitat; Alemania
Fil: Lutich, Andrey A.. Ludwig Maximilians Universitat; Alemania
Fil: Feldmann, Jochen. Ludwig Maximilians Universitat; Alemania
Materia
Biological Membranes
Membrane Composition
Microfluidic Jetting
Phospholipid Vesicles
Raman Scattering
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/68944
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/68944
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network_name_str CONICET Digital (CONICET)
spelling Membrane composition of jetted lipid vesicles: A Raman spectroscopy studyKirchner, Silke R.Ohlinger, AlexanderPfeiffer, TomUrban, Alexander S.Stefani, Fernando DanielDeak, AndrasLutich, Andrey A.Feldmann, JochenBiological MembranesMembrane CompositionMicrofluidic JettingPhospholipid VesiclesRaman Scatteringhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Microfluidic jetting is a promising method to produce giant unilamellar phospholipid vesicles for mimicking living cells in biomedical studies. We have investigated the chemical composition of membranes of vesicles prepared using this approach by means of Raman scattering spectroscopy. The membranes of all jetted vesicles are found to contain residuals of the organic solvent decane used in the preparation of the initial planar membrane. The decane inclusions are randomly distributed over the vesicle surface area and vary in thickness from a few to several tens of nanometers. Our findings point out that the membrane properties of jetted vesicles may differ considerably from those of vesicles prepared by other methods and from those of living cells.Fil: Kirchner, Silke R.. Ludwig Maximilians Universitat; AlemaniaFil: Ohlinger, Alexander. Ludwig Maximilians Universitat; AlemaniaFil: Pfeiffer, Tom. Ludwig Maximilians Universitat; AlemaniaFil: Urban, Alexander S.. Ludwig Maximilians Universitat; AlemaniaFil: Stefani, Fernando Daniel. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Deak, Andras. Ludwig Maximilians Universitat; AlemaniaFil: Lutich, Andrey A.. Ludwig Maximilians Universitat; AlemaniaFil: Feldmann, Jochen. Ludwig Maximilians Universitat; AlemaniaWiley VCH Verlag2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68944Kirchner, Silke R.; Ohlinger, Alexander; Pfeiffer, Tom; Urban, Alexander S.; Stefani, Fernando Daniel; et al.; Membrane composition of jetted lipid vesicles: A Raman spectroscopy study; Wiley VCH Verlag; Journal Of Biophotonics; 5; 1; 1-2012; 40-461864-063XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jbio.201100058info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201100058info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:51Zoai:ri.conicet.gov.ar:11336/68944instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:51.476CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Membrane composition of jetted lipid vesicles: A Raman spectroscopy study
title Membrane composition of jetted lipid vesicles: A Raman spectroscopy study
spellingShingle Membrane composition of jetted lipid vesicles: A Raman spectroscopy study
Kirchner, Silke R.
Biological Membranes
Membrane Composition
Microfluidic Jetting
Phospholipid Vesicles
Raman Scattering
title_short Membrane composition of jetted lipid vesicles: A Raman spectroscopy study
title_full Membrane composition of jetted lipid vesicles: A Raman spectroscopy study
title_fullStr Membrane composition of jetted lipid vesicles: A Raman spectroscopy study
title_full_unstemmed Membrane composition of jetted lipid vesicles: A Raman spectroscopy study
title_sort Membrane composition of jetted lipid vesicles: A Raman spectroscopy study
dc.creator.none.fl_str_mv Kirchner, Silke R.
Ohlinger, Alexander
Pfeiffer, Tom
Urban, Alexander S.
Stefani, Fernando Daniel
Deak, Andras
Lutich, Andrey A.
Feldmann, Jochen
author Kirchner, Silke R.
author_facet Kirchner, Silke R.
Ohlinger, Alexander
Pfeiffer, Tom
Urban, Alexander S.
Stefani, Fernando Daniel
Deak, Andras
Lutich, Andrey A.
Feldmann, Jochen
author_role author
author2 Ohlinger, Alexander
Pfeiffer, Tom
Urban, Alexander S.
Stefani, Fernando Daniel
Deak, Andras
Lutich, Andrey A.
Feldmann, Jochen
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biological Membranes
Membrane Composition
Microfluidic Jetting
Phospholipid Vesicles
Raman Scattering
topic Biological Membranes
Membrane Composition
Microfluidic Jetting
Phospholipid Vesicles
Raman Scattering
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microfluidic jetting is a promising method to produce giant unilamellar phospholipid vesicles for mimicking living cells in biomedical studies. We have investigated the chemical composition of membranes of vesicles prepared using this approach by means of Raman scattering spectroscopy. The membranes of all jetted vesicles are found to contain residuals of the organic solvent decane used in the preparation of the initial planar membrane. The decane inclusions are randomly distributed over the vesicle surface area and vary in thickness from a few to several tens of nanometers. Our findings point out that the membrane properties of jetted vesicles may differ considerably from those of vesicles prepared by other methods and from those of living cells.
Fil: Kirchner, Silke R.. Ludwig Maximilians Universitat; Alemania
Fil: Ohlinger, Alexander. Ludwig Maximilians Universitat; Alemania
Fil: Pfeiffer, Tom. Ludwig Maximilians Universitat; Alemania
Fil: Urban, Alexander S.. Ludwig Maximilians Universitat; Alemania
Fil: Stefani, Fernando Daniel. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Deak, Andras. Ludwig Maximilians Universitat; Alemania
Fil: Lutich, Andrey A.. Ludwig Maximilians Universitat; Alemania
Fil: Feldmann, Jochen. Ludwig Maximilians Universitat; Alemania
description Microfluidic jetting is a promising method to produce giant unilamellar phospholipid vesicles for mimicking living cells in biomedical studies. We have investigated the chemical composition of membranes of vesicles prepared using this approach by means of Raman scattering spectroscopy. The membranes of all jetted vesicles are found to contain residuals of the organic solvent decane used in the preparation of the initial planar membrane. The decane inclusions are randomly distributed over the vesicle surface area and vary in thickness from a few to several tens of nanometers. Our findings point out that the membrane properties of jetted vesicles may differ considerably from those of vesicles prepared by other methods and from those of living cells.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68944
Kirchner, Silke R.; Ohlinger, Alexander; Pfeiffer, Tom; Urban, Alexander S.; Stefani, Fernando Daniel; et al.; Membrane composition of jetted lipid vesicles: A Raman spectroscopy study; Wiley VCH Verlag; Journal Of Biophotonics; 5; 1; 1-2012; 40-46
1864-063X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68944
identifier_str_mv Kirchner, Silke R.; Ohlinger, Alexander; Pfeiffer, Tom; Urban, Alexander S.; Stefani, Fernando Daniel; et al.; Membrane composition of jetted lipid vesicles: A Raman spectroscopy study; Wiley VCH Verlag; Journal Of Biophotonics; 5; 1; 1-2012; 40-46
1864-063X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jbio.201100058
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201100058
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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