Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease

Autores
Vázquez, María Elisa; Mesias, Andrea Cecilia; Acuña, Leonardo; Spangler, Joseph; Zabala, Brenda Adriana; Parodi Ramoneda, Cecilia María; Thakur, Meghna; Oh, Eunkeu; Walper, Scott Allan; Pérez Brandan, Cecilia María
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND:Vaccine development is a laborious craftwork in which at least two main components must be defined: a highly immunogenic antigen and a suitable delivery method. Hence, the interplay of these elements could elicit the required immune response to cope with the targeted pathogen with a long-lasting protective capacity.OBJECTIVES:Here we evaluate the properties of Escherichia coli spherical proteoliposomes - known as outer membrane vesicles (OMVs) - as particles with natural adjuvant capacities and as antigen-carrier structures to assemble an innovative prophylactic vaccine for Chagas disease.METHODSTo achieve this, genetic manipulation was carried out on E. coli using an engineered plasmid containing the Tc24 Trypanosoma cruzi antigen. The goal was to induce the release of OMVs displaying the parasite protein on their surface.FINDINGS: As a proof of principle, we observed that native OMVs - as well as those carrying the T. cruzi antigen - were able to trigger a slight, but functional humoral response at low immunization doses. Of note, compared to the non-immunized group, native OMVs-vaccinated animals survived the lethal challenge and showed minor parasitemia values, suggesting a possible involvement of innate trained immunity mechanism.MAIN CONCLUSION: These results open the range for further research on the design of new carrier strategies focused on innate immunity activation as an additional immunization target and venture to seek for alternative forms in which OMVs could be used for optimizing vaccine development.
Fil: Vázquez, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Mesias, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Spangler, Joseph. Spece Sciences División. Naval Research Laboratory; Estados Unidos
Fil: Zabala, Brenda Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Parodi Ramoneda, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Thakur, Meghna. George Mason University; Estados Unidos
Fil: Oh, Eunkeu. Spece Sciences División. Naval Research Laboratory; Estados Unidos
Fil: Walper, Scott Allan. Spece Sciences División. Naval Research Laboratory; Estados Unidos
Fil: Pérez Brandan, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Materia
Outer Membrane Vesicles
Trypansosoma cruzi
Vaccine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230187
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas diseaseVázquez, María ElisaMesias, Andrea CeciliaAcuña, LeonardoSpangler, JosephZabala, Brenda AdrianaParodi Ramoneda, Cecilia MaríaThakur, MeghnaOh, EunkeuWalper, Scott AllanPérez Brandan, Cecilia MaríaOuter Membrane VesiclesTrypansosoma cruziVaccinehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3BACKGROUND:Vaccine development is a laborious craftwork in which at least two main components must be defined: a highly immunogenic antigen and a suitable delivery method. Hence, the interplay of these elements could elicit the required immune response to cope with the targeted pathogen with a long-lasting protective capacity.OBJECTIVES:Here we evaluate the properties of Escherichia coli spherical proteoliposomes - known as outer membrane vesicles (OMVs) - as particles with natural adjuvant capacities and as antigen-carrier structures to assemble an innovative prophylactic vaccine for Chagas disease.METHODSTo achieve this, genetic manipulation was carried out on E. coli using an engineered plasmid containing the Tc24 Trypanosoma cruzi antigen. The goal was to induce the release of OMVs displaying the parasite protein on their surface.FINDINGS: As a proof of principle, we observed that native OMVs - as well as those carrying the T. cruzi antigen - were able to trigger a slight, but functional humoral response at low immunization doses. Of note, compared to the non-immunized group, native OMVs-vaccinated animals survived the lethal challenge and showed minor parasitemia values, suggesting a possible involvement of innate trained immunity mechanism.MAIN CONCLUSION: These results open the range for further research on the design of new carrier strategies focused on innate immunity activation as an additional immunization target and venture to seek for alternative forms in which OMVs could be used for optimizing vaccine development.Fil: Vázquez, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Mesias, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Spangler, Joseph. Spece Sciences División. Naval Research Laboratory; Estados UnidosFil: Zabala, Brenda Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Parodi Ramoneda, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Thakur, Meghna. George Mason University; Estados UnidosFil: Oh, Eunkeu. Spece Sciences División. Naval Research Laboratory; Estados UnidosFil: Walper, Scott Allan. Spece Sciences División. Naval Research Laboratory; Estados UnidosFil: Pérez Brandan, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFundação Oswaldo Cruz2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230187Vázquez, María Elisa; Mesias, Andrea Cecilia; Acuña, Leonardo; Spangler, Joseph; Zabala, Brenda Adriana; et al.; Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 118; 5-2023; 1-120074-02761678-8060CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760220263info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:12:32Zoai:ri.conicet.gov.ar:11336/230187instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:12:32.343CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease
title Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease
spellingShingle Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease
Vázquez, María Elisa
Outer Membrane Vesicles
Trypansosoma cruzi
Vaccine
title_short Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease
title_full Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease
title_fullStr Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease
title_full_unstemmed Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease
title_sort Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease
dc.creator.none.fl_str_mv Vázquez, María Elisa
Mesias, Andrea Cecilia
Acuña, Leonardo
Spangler, Joseph
Zabala, Brenda Adriana
Parodi Ramoneda, Cecilia María
Thakur, Meghna
Oh, Eunkeu
Walper, Scott Allan
Pérez Brandan, Cecilia María
author Vázquez, María Elisa
author_facet Vázquez, María Elisa
Mesias, Andrea Cecilia
Acuña, Leonardo
Spangler, Joseph
Zabala, Brenda Adriana
Parodi Ramoneda, Cecilia María
Thakur, Meghna
Oh, Eunkeu
Walper, Scott Allan
Pérez Brandan, Cecilia María
author_role author
author2 Mesias, Andrea Cecilia
Acuña, Leonardo
Spangler, Joseph
Zabala, Brenda Adriana
Parodi Ramoneda, Cecilia María
Thakur, Meghna
Oh, Eunkeu
Walper, Scott Allan
Pérez Brandan, Cecilia María
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Outer Membrane Vesicles
Trypansosoma cruzi
Vaccine
topic Outer Membrane Vesicles
Trypansosoma cruzi
Vaccine
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv BACKGROUND:Vaccine development is a laborious craftwork in which at least two main components must be defined: a highly immunogenic antigen and a suitable delivery method. Hence, the interplay of these elements could elicit the required immune response to cope with the targeted pathogen with a long-lasting protective capacity.OBJECTIVES:Here we evaluate the properties of Escherichia coli spherical proteoliposomes - known as outer membrane vesicles (OMVs) - as particles with natural adjuvant capacities and as antigen-carrier structures to assemble an innovative prophylactic vaccine for Chagas disease.METHODSTo achieve this, genetic manipulation was carried out on E. coli using an engineered plasmid containing the Tc24 Trypanosoma cruzi antigen. The goal was to induce the release of OMVs displaying the parasite protein on their surface.FINDINGS: As a proof of principle, we observed that native OMVs - as well as those carrying the T. cruzi antigen - were able to trigger a slight, but functional humoral response at low immunization doses. Of note, compared to the non-immunized group, native OMVs-vaccinated animals survived the lethal challenge and showed minor parasitemia values, suggesting a possible involvement of innate trained immunity mechanism.MAIN CONCLUSION: These results open the range for further research on the design of new carrier strategies focused on innate immunity activation as an additional immunization target and venture to seek for alternative forms in which OMVs could be used for optimizing vaccine development.
Fil: Vázquez, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Mesias, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Spangler, Joseph. Spece Sciences División. Naval Research Laboratory; Estados Unidos
Fil: Zabala, Brenda Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Parodi Ramoneda, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Thakur, Meghna. George Mason University; Estados Unidos
Fil: Oh, Eunkeu. Spece Sciences División. Naval Research Laboratory; Estados Unidos
Fil: Walper, Scott Allan. Spece Sciences División. Naval Research Laboratory; Estados Unidos
Fil: Pérez Brandan, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
description BACKGROUND:Vaccine development is a laborious craftwork in which at least two main components must be defined: a highly immunogenic antigen and a suitable delivery method. Hence, the interplay of these elements could elicit the required immune response to cope with the targeted pathogen with a long-lasting protective capacity.OBJECTIVES:Here we evaluate the properties of Escherichia coli spherical proteoliposomes - known as outer membrane vesicles (OMVs) - as particles with natural adjuvant capacities and as antigen-carrier structures to assemble an innovative prophylactic vaccine for Chagas disease.METHODSTo achieve this, genetic manipulation was carried out on E. coli using an engineered plasmid containing the Tc24 Trypanosoma cruzi antigen. The goal was to induce the release of OMVs displaying the parasite protein on their surface.FINDINGS: As a proof of principle, we observed that native OMVs - as well as those carrying the T. cruzi antigen - were able to trigger a slight, but functional humoral response at low immunization doses. Of note, compared to the non-immunized group, native OMVs-vaccinated animals survived the lethal challenge and showed minor parasitemia values, suggesting a possible involvement of innate trained immunity mechanism.MAIN CONCLUSION: These results open the range for further research on the design of new carrier strategies focused on innate immunity activation as an additional immunization target and venture to seek for alternative forms in which OMVs could be used for optimizing vaccine development.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230187
Vázquez, María Elisa; Mesias, Andrea Cecilia; Acuña, Leonardo; Spangler, Joseph; Zabala, Brenda Adriana; et al.; Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 118; 5-2023; 1-12
0074-0276
1678-8060
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230187
identifier_str_mv Vázquez, María Elisa; Mesias, Andrea Cecilia; Acuña, Leonardo; Spangler, Joseph; Zabala, Brenda Adriana; et al.; Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 118; 5-2023; 1-12
0074-0276
1678-8060
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760220263
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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