Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells

Autores
Rodríguez, Tania Melina; Saldias. Alejandro; Irigo, Marcelo; Velasco Zamora, Jorge; Perone, Marcelo Javier; Dewey, Ricardo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adipose tissue is an attractive source of mesenchymal stromal cells (MSCs) owing to the relative ease of obtaining large volumes with more MSC abundance compared with other sources. Increasing evidence supports the fact that trophic factors secreted by MSCs play a pivotal therapeutic role. Several strategies in regenerative medicine use MSCs, mainly exploiting their immunosuppressive effect and homing capacity to sites of damage. Transforming growth factor-b1 (TGF-b1) is a pleiotropic cytokine that, depending on the cell niche, can display either anti-inflammatory or proinflammatory effects. TGF-b1 expression increases in various tissues with damage, especially when accompanied by inflammation. Thus, we analyzed the effect of TGF-b1 on the secretion by adipose-derived mesenchymal stromal cells (ASCs) of a panel of 80 cytokines/chemokines using an antibody array. To avoid a possible effect of fetal bovine serum (FBS) on ASCs secretion, we performed our analysis by culturing cells in FBS-free conditions, only supplemented with 0.1% of bovine serum albumin. We report the cytokine profile secreted by ASCs. We also found that TGF-b1 exposure modulates 8 chemokines and 18 cytokines, including TGF-b1 and -b2, and other important cytokines involved in immunosuppression, allergic responses, and bone resorption.
Fil: Rodríguez, Tania Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina. Universidad Nacional de San Martín; Argentina
Fil: Saldias. Alejandro. Hospital Italiano de Buenos Aires; Argentina
Fil: Irigo, Marcelo. Hospital Italiano de Buenos Aires; Argentina
Fil: Velasco Zamora, Jorge. Fundación Articular; Argentina
Fil: Perone, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires; Argentina
Fil: Dewey, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina. Universidad Nacional de San Martín; Argentina
Materia
Mscs
Mesenchymal Stromal Cells
Secretion of Cytokines And Chemokines
Secretome
Transforming Growth Factor-B1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13742
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13742
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cellsRodríguez, Tania MelinaSaldias. AlejandroIrigo, MarceloVelasco Zamora, JorgePerone, Marcelo JavierDewey, RicardoMscsMesenchymal Stromal CellsSecretion of Cytokines And ChemokinesSecretomeTransforming Growth Factor-B1https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Adipose tissue is an attractive source of mesenchymal stromal cells (MSCs) owing to the relative ease of obtaining large volumes with more MSC abundance compared with other sources. Increasing evidence supports the fact that trophic factors secreted by MSCs play a pivotal therapeutic role. Several strategies in regenerative medicine use MSCs, mainly exploiting their immunosuppressive effect and homing capacity to sites of damage. Transforming growth factor-b1 (TGF-b1) is a pleiotropic cytokine that, depending on the cell niche, can display either anti-inflammatory or proinflammatory effects. TGF-b1 expression increases in various tissues with damage, especially when accompanied by inflammation. Thus, we analyzed the effect of TGF-b1 on the secretion by adipose-derived mesenchymal stromal cells (ASCs) of a panel of 80 cytokines/chemokines using an antibody array. To avoid a possible effect of fetal bovine serum (FBS) on ASCs secretion, we performed our analysis by culturing cells in FBS-free conditions, only supplemented with 0.1% of bovine serum albumin. We report the cytokine profile secreted by ASCs. We also found that TGF-b1 exposure modulates 8 chemokines and 18 cytokines, including TGF-b1 and -b2, and other important cytokines involved in immunosuppression, allergic responses, and bone resorption.Fil: Rodríguez, Tania Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina. Universidad Nacional de San Martín; ArgentinaFil: Saldias. Alejandro. Hospital Italiano de Buenos Aires; ArgentinaFil: Irigo, Marcelo. Hospital Italiano de Buenos Aires; ArgentinaFil: Velasco Zamora, Jorge. Fundación Articular; ArgentinaFil: Perone, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires; ArgentinaFil: Dewey, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina. Universidad Nacional de San Martín; ArgentinaWiley2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13742Rodríguez, Tania Melina; Saldias. Alejandro; Irigo, Marcelo; Velasco Zamora, Jorge; Perone, Marcelo Javier; et al.; Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells; Wiley; Stem Cells; 4; 8; 8-2015; 894-8982157-6564enginfo:eu-repo/semantics/altIdentifier/doi/10.5966/sctm.2015-0012info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.5966/sctm.2015-0012/abstractinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511148/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:20:58Zoai:ri.conicet.gov.ar:11336/13742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:20:59.16CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells
title Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells
spellingShingle Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells
Rodríguez, Tania Melina
Mscs
Mesenchymal Stromal Cells
Secretion of Cytokines And Chemokines
Secretome
Transforming Growth Factor-B1
title_short Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells
title_full Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells
title_fullStr Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells
title_full_unstemmed Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells
title_sort Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells
dc.creator.none.fl_str_mv Rodríguez, Tania Melina
Saldias. Alejandro
Irigo, Marcelo
Velasco Zamora, Jorge
Perone, Marcelo Javier
Dewey, Ricardo
author Rodríguez, Tania Melina
author_facet Rodríguez, Tania Melina
Saldias. Alejandro
Irigo, Marcelo
Velasco Zamora, Jorge
Perone, Marcelo Javier
Dewey, Ricardo
author_role author
author2 Saldias. Alejandro
Irigo, Marcelo
Velasco Zamora, Jorge
Perone, Marcelo Javier
Dewey, Ricardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Mscs
Mesenchymal Stromal Cells
Secretion of Cytokines And Chemokines
Secretome
Transforming Growth Factor-B1
topic Mscs
Mesenchymal Stromal Cells
Secretion of Cytokines And Chemokines
Secretome
Transforming Growth Factor-B1
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Adipose tissue is an attractive source of mesenchymal stromal cells (MSCs) owing to the relative ease of obtaining large volumes with more MSC abundance compared with other sources. Increasing evidence supports the fact that trophic factors secreted by MSCs play a pivotal therapeutic role. Several strategies in regenerative medicine use MSCs, mainly exploiting their immunosuppressive effect and homing capacity to sites of damage. Transforming growth factor-b1 (TGF-b1) is a pleiotropic cytokine that, depending on the cell niche, can display either anti-inflammatory or proinflammatory effects. TGF-b1 expression increases in various tissues with damage, especially when accompanied by inflammation. Thus, we analyzed the effect of TGF-b1 on the secretion by adipose-derived mesenchymal stromal cells (ASCs) of a panel of 80 cytokines/chemokines using an antibody array. To avoid a possible effect of fetal bovine serum (FBS) on ASCs secretion, we performed our analysis by culturing cells in FBS-free conditions, only supplemented with 0.1% of bovine serum albumin. We report the cytokine profile secreted by ASCs. We also found that TGF-b1 exposure modulates 8 chemokines and 18 cytokines, including TGF-b1 and -b2, and other important cytokines involved in immunosuppression, allergic responses, and bone resorption.
Fil: Rodríguez, Tania Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina. Universidad Nacional de San Martín; Argentina
Fil: Saldias. Alejandro. Hospital Italiano de Buenos Aires; Argentina
Fil: Irigo, Marcelo. Hospital Italiano de Buenos Aires; Argentina
Fil: Velasco Zamora, Jorge. Fundación Articular; Argentina
Fil: Perone, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires; Argentina
Fil: Dewey, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina. Universidad Nacional de San Martín; Argentina
description Adipose tissue is an attractive source of mesenchymal stromal cells (MSCs) owing to the relative ease of obtaining large volumes with more MSC abundance compared with other sources. Increasing evidence supports the fact that trophic factors secreted by MSCs play a pivotal therapeutic role. Several strategies in regenerative medicine use MSCs, mainly exploiting their immunosuppressive effect and homing capacity to sites of damage. Transforming growth factor-b1 (TGF-b1) is a pleiotropic cytokine that, depending on the cell niche, can display either anti-inflammatory or proinflammatory effects. TGF-b1 expression increases in various tissues with damage, especially when accompanied by inflammation. Thus, we analyzed the effect of TGF-b1 on the secretion by adipose-derived mesenchymal stromal cells (ASCs) of a panel of 80 cytokines/chemokines using an antibody array. To avoid a possible effect of fetal bovine serum (FBS) on ASCs secretion, we performed our analysis by culturing cells in FBS-free conditions, only supplemented with 0.1% of bovine serum albumin. We report the cytokine profile secreted by ASCs. We also found that TGF-b1 exposure modulates 8 chemokines and 18 cytokines, including TGF-b1 and -b2, and other important cytokines involved in immunosuppression, allergic responses, and bone resorption.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13742
Rodríguez, Tania Melina; Saldias. Alejandro; Irigo, Marcelo; Velasco Zamora, Jorge; Perone, Marcelo Javier; et al.; Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells; Wiley; Stem Cells; 4; 8; 8-2015; 894-898
2157-6564
url http://hdl.handle.net/11336/13742
identifier_str_mv Rodríguez, Tania Melina; Saldias. Alejandro; Irigo, Marcelo; Velasco Zamora, Jorge; Perone, Marcelo Javier; et al.; Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells; Wiley; Stem Cells; 4; 8; 8-2015; 894-898
2157-6564
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5966/sctm.2015-0012
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.5966/sctm.2015-0012/abstract
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511148/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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