Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment

Autores
Ernst, Glenda; Jancic, Carolina Cristina; Auteri, Santiago; Rodriguez Moncalvo, Juan; Galíndez, Fernando; Grynblat, Pedro; Hajos, Silvia Elvira
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose: Interstitial Lung Diseases (ILD) are characterized by inflammation and fibrosis. It described the role of hyaluronic acid (HA) as an immune-regulator. It is not known if HA contributes to the recruitment of inflammatory cells associated with ILD. If this hypothesis was correct, then concentrations of HA in bronchoalveolar lavage (BAL) should correlate with the severity of ILD. Methods: We collected BAL from 22 ILD patients and 15 control subjects. We determined HA and cytokine levels by ELISA. In vitro chemotaxis assays were performed by using a transwell system. Results: We found that ILD patients showed a significant increase in HA, IL-6 levels and the amount of cells in BAL compared to control subjects. We detected a significant positive correlation between HA and IL-6 levels (r = 0.53 and p < 0.001) and an inverse relationship between HA levels and diffusion capacity (r = -0.59, p < 0.01). In vitro, HA induced migration of macrophages and monocytes through a CD44-dependent process. BAL from patients with ILD stimulated macro-phage migration and this was abrogated by hyaluronidase. Conclusions: Our results support the hypothesis that HA contributes to the recruitment of monocytes towards the alveolar space, leading to exacerbation of lung inflammation in ILD patients.
Fil: Ernst, Glenda. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; Argentina
Fil: Jancic, Carolina Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Auteri, Santiago. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; Argentina
Fil: Rodriguez Moncalvo, Juan. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; Argentina
Fil: Galíndez, Fernando. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; Argentina
Fil: Grynblat, Pedro. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; Argentina
Fil: Hajos, Silvia Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Materia
HYALURONIC ACID
BRONCHOALVEOLAR LAVAGE
INTERSTITAL LUNG DESEASES
INFLAMMATORY CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29413
Acceder
id CONICETDig_18cc47d824000cda34e914885e052355
oai_identifier_str oai:ri.conicet.gov.ar:11336/29413
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitmentErnst, GlendaJancic, Carolina CristinaAuteri, SantiagoRodriguez Moncalvo, JuanGalíndez, FernandoGrynblat, PedroHajos, Silvia ElviraHYALURONIC ACIDBRONCHOALVEOLAR LAVAGEINTERSTITAL LUNG DESEASESINFLAMMATORY CELLShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Purpose: Interstitial Lung Diseases (ILD) are characterized by inflammation and fibrosis. It described the role of hyaluronic acid (HA) as an immune-regulator. It is not known if HA contributes to the recruitment of inflammatory cells associated with ILD. If this hypothesis was correct, then concentrations of HA in bronchoalveolar lavage (BAL) should correlate with the severity of ILD. Methods: We collected BAL from 22 ILD patients and 15 control subjects. We determined HA and cytokine levels by ELISA. In vitro chemotaxis assays were performed by using a transwell system. Results: We found that ILD patients showed a significant increase in HA, IL-6 levels and the amount of cells in BAL compared to control subjects. We detected a significant positive correlation between HA and IL-6 levels (r = 0.53 and p < 0.001) and an inverse relationship between HA levels and diffusion capacity (r = -0.59, p < 0.01). In vitro, HA induced migration of macrophages and monocytes through a CD44-dependent process. BAL from patients with ILD stimulated macro-phage migration and this was abrogated by hyaluronidase. Conclusions: Our results support the hypothesis that HA contributes to the recruitment of monocytes towards the alveolar space, leading to exacerbation of lung inflammation in ILD patients.Fil: Ernst, Glenda. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; ArgentinaFil: Jancic, Carolina Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Auteri, Santiago. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; ArgentinaFil: Rodriguez Moncalvo, Juan. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; ArgentinaFil: Galíndez, Fernando. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; ArgentinaFil: Grynblat, Pedro. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; ArgentinaFil: Hajos, Silvia Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaScientific Research Publishing2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29413Ernst, Glenda; Jancic, Carolina Cristina; Auteri, Santiago; Rodriguez Moncalvo, Juan; Galíndez, Fernando; et al.; Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment; Scientific Research Publishing; Modern Research in Inflammation; 3; 2; 5-2014; 27-362169-9682CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4236/mri.2014.32004info:eu-repo/semantics/altIdentifier/url/https://www.scirp.org/journal/PaperInformation.aspx?PaperID=45076info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:28:11Zoai:ri.conicet.gov.ar:11336/29413instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:28:11.966CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment
title Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment
spellingShingle Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment
Ernst, Glenda
HYALURONIC ACID
BRONCHOALVEOLAR LAVAGE
INTERSTITAL LUNG DESEASES
INFLAMMATORY CELLS
title_short Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment
title_full Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment
title_fullStr Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment
title_full_unstemmed Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment
title_sort Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment
dc.creator.none.fl_str_mv Ernst, Glenda
Jancic, Carolina Cristina
Auteri, Santiago
Rodriguez Moncalvo, Juan
Galíndez, Fernando
Grynblat, Pedro
Hajos, Silvia Elvira
author Ernst, Glenda
author_facet Ernst, Glenda
Jancic, Carolina Cristina
Auteri, Santiago
Rodriguez Moncalvo, Juan
Galíndez, Fernando
Grynblat, Pedro
Hajos, Silvia Elvira
author_role author
author2 Jancic, Carolina Cristina
Auteri, Santiago
Rodriguez Moncalvo, Juan
Galíndez, Fernando
Grynblat, Pedro
Hajos, Silvia Elvira
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv HYALURONIC ACID
BRONCHOALVEOLAR LAVAGE
INTERSTITAL LUNG DESEASES
INFLAMMATORY CELLS
topic HYALURONIC ACID
BRONCHOALVEOLAR LAVAGE
INTERSTITAL LUNG DESEASES
INFLAMMATORY CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Purpose: Interstitial Lung Diseases (ILD) are characterized by inflammation and fibrosis. It described the role of hyaluronic acid (HA) as an immune-regulator. It is not known if HA contributes to the recruitment of inflammatory cells associated with ILD. If this hypothesis was correct, then concentrations of HA in bronchoalveolar lavage (BAL) should correlate with the severity of ILD. Methods: We collected BAL from 22 ILD patients and 15 control subjects. We determined HA and cytokine levels by ELISA. In vitro chemotaxis assays were performed by using a transwell system. Results: We found that ILD patients showed a significant increase in HA, IL-6 levels and the amount of cells in BAL compared to control subjects. We detected a significant positive correlation between HA and IL-6 levels (r = 0.53 and p < 0.001) and an inverse relationship between HA levels and diffusion capacity (r = -0.59, p < 0.01). In vitro, HA induced migration of macrophages and monocytes through a CD44-dependent process. BAL from patients with ILD stimulated macro-phage migration and this was abrogated by hyaluronidase. Conclusions: Our results support the hypothesis that HA contributes to the recruitment of monocytes towards the alveolar space, leading to exacerbation of lung inflammation in ILD patients.
Fil: Ernst, Glenda. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; Argentina
Fil: Jancic, Carolina Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Auteri, Santiago. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; Argentina
Fil: Rodriguez Moncalvo, Juan. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; Argentina
Fil: Galíndez, Fernando. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; Argentina
Fil: Grynblat, Pedro. Ciudad Autónoma de Buenos Aires. Hospital María Ferrer; Argentina
Fil: Hajos, Silvia Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
description Purpose: Interstitial Lung Diseases (ILD) are characterized by inflammation and fibrosis. It described the role of hyaluronic acid (HA) as an immune-regulator. It is not known if HA contributes to the recruitment of inflammatory cells associated with ILD. If this hypothesis was correct, then concentrations of HA in bronchoalveolar lavage (BAL) should correlate with the severity of ILD. Methods: We collected BAL from 22 ILD patients and 15 control subjects. We determined HA and cytokine levels by ELISA. In vitro chemotaxis assays were performed by using a transwell system. Results: We found that ILD patients showed a significant increase in HA, IL-6 levels and the amount of cells in BAL compared to control subjects. We detected a significant positive correlation between HA and IL-6 levels (r = 0.53 and p < 0.001) and an inverse relationship between HA levels and diffusion capacity (r = -0.59, p < 0.01). In vitro, HA induced migration of macrophages and monocytes through a CD44-dependent process. BAL from patients with ILD stimulated macro-phage migration and this was abrogated by hyaluronidase. Conclusions: Our results support the hypothesis that HA contributes to the recruitment of monocytes towards the alveolar space, leading to exacerbation of lung inflammation in ILD patients.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29413
Ernst, Glenda; Jancic, Carolina Cristina; Auteri, Santiago; Rodriguez Moncalvo, Juan; Galíndez, Fernando; et al.; Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment; Scientific Research Publishing; Modern Research in Inflammation; 3; 2; 5-2014; 27-36
2169-9682
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29413
identifier_str_mv Ernst, Glenda; Jancic, Carolina Cristina; Auteri, Santiago; Rodriguez Moncalvo, Juan; Galíndez, Fernando; et al.; Increased levels of hyaluronic acid in bronchoalveolar lavage from patients with interstitial lung diseases, relationship with lung function and inflammatory cells recruitment; Scientific Research Publishing; Modern Research in Inflammation; 3; 2; 5-2014; 27-36
2169-9682
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4236/mri.2014.32004
info:eu-repo/semantics/altIdentifier/url/https://www.scirp.org/journal/PaperInformation.aspx?PaperID=45076
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research Publishing
publisher.none.fl_str_mv Scientific Research Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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