Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages

Autores
Sánchez López, Luna; Ropero de Torres, Noelia; Chico, Belén; Fagali, Natalia Soledad; De los Ríos, Vivian; Escudero, María Lorenza; García-Alonso, María Cristina; Lozano Puerto, Rosa María
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The presence of a worn surface in the implanted material, as in the case of a replacement of a damaged osteoarticular joint, is the normal condition after implantation. This manuscript focuses precisely on the comparative study of the cellular behavior on worn CoCr surfaces, analyzing the effect of different surface modifications on macrophages’ responses. CoCr surfaces were modified by the deposition of electrochemically reduced graphene oxide (CoCrErGO), followed by additional surface functionalization with hyaluronic acid (CoCrErGOHA). After the wear corrosion processes, the macrophage response was studied. In addition, macrophage supernatants exposed to the surfaces, before and after wear, were also evaluated for osteoblast response through the analysis of the metabolic activity, plasma membrane damage, and phosphatase alkaline activity (ALP). The proteomic analysis and the quantitative TNF-α/IL-10 ratios of the J774A.1 macrophages exposed to the surfaces under study showed a polarization shift from M0 (basal state) to M1, associated with the pro-inflammatory response of all surfaces. A lower M1 polarization was observed upon exposure to the surface modification with ErGO, whereas posterior HA functionalization attenuated, even more, the M1 polarization. The wear corrosion process contributed to inflammation and exacerbated the M1 polarization response on macrophages to CoCr, which was diminished for the ErGO and attenuated the most for the ErGOHA surfaces. Comparative proteomics showed that the pathways related to M1 polarization were downregulated on the surfaces of CoCrErGOHA, which suggests mechanisms for the observed attenuation of M1 polarization. The suitable immuno-modulatory potential induced by the ErGOHA surface, with and without wear, together with the stimulation of ALP activity in osteoblasts induced by macrophage supernatants, promotes the mineralization processes necessary for bone repair. This makes it feasible to consider the adsorption of ErGOHA on CoCr as a recommended surface treatment for the use of biomaterials in osseous joint applications.
Fil: Sánchez López, Luna. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; España. Universidad Autónoma de Madrid; España
Fil: Ropero de Torres, Noelia. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Chico, Belén. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; España
Fil: Fagali, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: De los Ríos, Vivian. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Escudero, María Lorenza. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; España
Fil: García-Alonso, María Cristina. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; España
Fil: Lozano Puerto, Rosa María. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Materia
COCR
GRAPHENE
HYALURONIC ACID
INFLAMMATORY RESPONSE
PROTEOMIC ANALYSIS
WEAR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227090
Acceder
id CONICETDig_83412af284460756db805da76fc232d9
oai_identifier_str oai:ri.conicet.gov.ar:11336/227090
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 MacrophagesSánchez López, LunaRopero de Torres, NoeliaChico, BelénFagali, Natalia SoledadDe los Ríos, VivianEscudero, María LorenzaGarcía-Alonso, María CristinaLozano Puerto, Rosa MaríaCOCRGRAPHENEHYALURONIC ACIDINFLAMMATORY RESPONSEPROTEOMIC ANALYSISWEARhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The presence of a worn surface in the implanted material, as in the case of a replacement of a damaged osteoarticular joint, is the normal condition after implantation. This manuscript focuses precisely on the comparative study of the cellular behavior on worn CoCr surfaces, analyzing the effect of different surface modifications on macrophages’ responses. CoCr surfaces were modified by the deposition of electrochemically reduced graphene oxide (CoCrErGO), followed by additional surface functionalization with hyaluronic acid (CoCrErGOHA). After the wear corrosion processes, the macrophage response was studied. In addition, macrophage supernatants exposed to the surfaces, before and after wear, were also evaluated for osteoblast response through the analysis of the metabolic activity, plasma membrane damage, and phosphatase alkaline activity (ALP). The proteomic analysis and the quantitative TNF-α/IL-10 ratios of the J774A.1 macrophages exposed to the surfaces under study showed a polarization shift from M0 (basal state) to M1, associated with the pro-inflammatory response of all surfaces. A lower M1 polarization was observed upon exposure to the surface modification with ErGO, whereas posterior HA functionalization attenuated, even more, the M1 polarization. The wear corrosion process contributed to inflammation and exacerbated the M1 polarization response on macrophages to CoCr, which was diminished for the ErGO and attenuated the most for the ErGOHA surfaces. Comparative proteomics showed that the pathways related to M1 polarization were downregulated on the surfaces of CoCrErGOHA, which suggests mechanisms for the observed attenuation of M1 polarization. The suitable immuno-modulatory potential induced by the ErGOHA surface, with and without wear, together with the stimulation of ALP activity in osteoblasts induced by macrophage supernatants, promotes the mineralization processes necessary for bone repair. This makes it feasible to consider the adsorption of ErGOHA on CoCr as a recommended surface treatment for the use of biomaterials in osseous joint applications.Fil: Sánchez López, Luna. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; España. Universidad Autónoma de Madrid; EspañaFil: Ropero de Torres, Noelia. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaFil: Chico, Belén. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; EspañaFil: Fagali, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: De los Ríos, Vivian. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaFil: Escudero, María Lorenza. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; EspañaFil: García-Alonso, María Cristina. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; EspañaFil: Lozano Puerto, Rosa María. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaMultidisciplinary Digital Publishing Institute2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227090Sánchez López, Luna; Ropero de Torres, Noelia; Chico, Belén; Fagali, Natalia Soledad; De los Ríos, Vivian; et al.; Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages; Multidisciplinary Digital Publishing Institute; Metals; 13; 3; 3-2023; 1-282075-4701CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-4701/13/3/598info:eu-repo/semantics/altIdentifier/doi/10.3390/met13030598info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:28:48Zoai:ri.conicet.gov.ar:11336/227090instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:28:48.311CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages
title Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages
spellingShingle Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages
Sánchez López, Luna
COCR
GRAPHENE
HYALURONIC ACID
INFLAMMATORY RESPONSE
PROTEOMIC ANALYSIS
WEAR
title_short Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages
title_full Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages
title_fullStr Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages
title_full_unstemmed Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages
title_sort Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages
dc.creator.none.fl_str_mv Sánchez López, Luna
Ropero de Torres, Noelia
Chico, Belén
Fagali, Natalia Soledad
De los Ríos, Vivian
Escudero, María Lorenza
García-Alonso, María Cristina
Lozano Puerto, Rosa María
author Sánchez López, Luna
author_facet Sánchez López, Luna
Ropero de Torres, Noelia
Chico, Belén
Fagali, Natalia Soledad
De los Ríos, Vivian
Escudero, María Lorenza
García-Alonso, María Cristina
Lozano Puerto, Rosa María
author_role author
author2 Ropero de Torres, Noelia
Chico, Belén
Fagali, Natalia Soledad
De los Ríos, Vivian
Escudero, María Lorenza
García-Alonso, María Cristina
Lozano Puerto, Rosa María
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COCR
GRAPHENE
HYALURONIC ACID
INFLAMMATORY RESPONSE
PROTEOMIC ANALYSIS
WEAR
topic COCR
GRAPHENE
HYALURONIC ACID
INFLAMMATORY RESPONSE
PROTEOMIC ANALYSIS
WEAR
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The presence of a worn surface in the implanted material, as in the case of a replacement of a damaged osteoarticular joint, is the normal condition after implantation. This manuscript focuses precisely on the comparative study of the cellular behavior on worn CoCr surfaces, analyzing the effect of different surface modifications on macrophages’ responses. CoCr surfaces were modified by the deposition of electrochemically reduced graphene oxide (CoCrErGO), followed by additional surface functionalization with hyaluronic acid (CoCrErGOHA). After the wear corrosion processes, the macrophage response was studied. In addition, macrophage supernatants exposed to the surfaces, before and after wear, were also evaluated for osteoblast response through the analysis of the metabolic activity, plasma membrane damage, and phosphatase alkaline activity (ALP). The proteomic analysis and the quantitative TNF-α/IL-10 ratios of the J774A.1 macrophages exposed to the surfaces under study showed a polarization shift from M0 (basal state) to M1, associated with the pro-inflammatory response of all surfaces. A lower M1 polarization was observed upon exposure to the surface modification with ErGO, whereas posterior HA functionalization attenuated, even more, the M1 polarization. The wear corrosion process contributed to inflammation and exacerbated the M1 polarization response on macrophages to CoCr, which was diminished for the ErGO and attenuated the most for the ErGOHA surfaces. Comparative proteomics showed that the pathways related to M1 polarization were downregulated on the surfaces of CoCrErGOHA, which suggests mechanisms for the observed attenuation of M1 polarization. The suitable immuno-modulatory potential induced by the ErGOHA surface, with and without wear, together with the stimulation of ALP activity in osteoblasts induced by macrophage supernatants, promotes the mineralization processes necessary for bone repair. This makes it feasible to consider the adsorption of ErGOHA on CoCr as a recommended surface treatment for the use of biomaterials in osseous joint applications.
Fil: Sánchez López, Luna. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; España. Universidad Autónoma de Madrid; España
Fil: Ropero de Torres, Noelia. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Chico, Belén. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; España
Fil: Fagali, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: De los Ríos, Vivian. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Escudero, María Lorenza. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; España
Fil: García-Alonso, María Cristina. Consejo Superior de Investigaciones Científicas. Centro Nacional de Investigaciones Metalúrgicas; España
Fil: Lozano Puerto, Rosa María. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
description The presence of a worn surface in the implanted material, as in the case of a replacement of a damaged osteoarticular joint, is the normal condition after implantation. This manuscript focuses precisely on the comparative study of the cellular behavior on worn CoCr surfaces, analyzing the effect of different surface modifications on macrophages’ responses. CoCr surfaces were modified by the deposition of electrochemically reduced graphene oxide (CoCrErGO), followed by additional surface functionalization with hyaluronic acid (CoCrErGOHA). After the wear corrosion processes, the macrophage response was studied. In addition, macrophage supernatants exposed to the surfaces, before and after wear, were also evaluated for osteoblast response through the analysis of the metabolic activity, plasma membrane damage, and phosphatase alkaline activity (ALP). The proteomic analysis and the quantitative TNF-α/IL-10 ratios of the J774A.1 macrophages exposed to the surfaces under study showed a polarization shift from M0 (basal state) to M1, associated with the pro-inflammatory response of all surfaces. A lower M1 polarization was observed upon exposure to the surface modification with ErGO, whereas posterior HA functionalization attenuated, even more, the M1 polarization. The wear corrosion process contributed to inflammation and exacerbated the M1 polarization response on macrophages to CoCr, which was diminished for the ErGO and attenuated the most for the ErGOHA surfaces. Comparative proteomics showed that the pathways related to M1 polarization were downregulated on the surfaces of CoCrErGOHA, which suggests mechanisms for the observed attenuation of M1 polarization. The suitable immuno-modulatory potential induced by the ErGOHA surface, with and without wear, together with the stimulation of ALP activity in osteoblasts induced by macrophage supernatants, promotes the mineralization processes necessary for bone repair. This makes it feasible to consider the adsorption of ErGOHA on CoCr as a recommended surface treatment for the use of biomaterials in osseous joint applications.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227090
Sánchez López, Luna; Ropero de Torres, Noelia; Chico, Belén; Fagali, Natalia Soledad; De los Ríos, Vivian; et al.; Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages; Multidisciplinary Digital Publishing Institute; Metals; 13; 3; 3-2023; 1-28
2075-4701
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227090
identifier_str_mv Sánchez López, Luna; Ropero de Torres, Noelia; Chico, Belén; Fagali, Natalia Soledad; De los Ríos, Vivian; et al.; Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages; Multidisciplinary Digital Publishing Institute; Metals; 13; 3; 3-2023; 1-28
2075-4701
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-4701/13/3/598
info:eu-repo/semantics/altIdentifier/doi/10.3390/met13030598
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.959664

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