Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses

Autores
Barrionuevo, Pablo Alejandro; Nicandro, Nathaniel; McAnany, J. Jason; Zele, Andrew J.; Gamlin, Paul; Cao, Dingcai
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose: To determine the relative contributions of rods, cones and melanopsin to pupil responses in humans using temporal sinusoidal stimulation for light levels spanning the low mesopic to photopic range. Methods: A four-primary Ganzfeld photostimulator controlled flicker stimulations at seven light levels (-2.7 to 2 log cd/m2) and five frequencies (0.5 to 8Hz). Pupil diameter was measured using a high-resolution eyetracker. Three kinds of sinusoidal photoreceptor modulations were generated using silent substitution: 1) rod modulation, 2) cone modulation, and 3) combined rod and cone modulation in phase (Experiment 1) or phase shifted (Experiment 2) from a fixed rod phase. The melanopsin excitation was computed for each condition. A vector sum model was used to estimate the relative contribution of rods, cones and melanopsin to the pupil response. Results: From Experiment 1, the pupil frequency response peaked at 1Hz at two mesopic light levels for the three modulation conditions. Analyzing the rod-cone phase difference for the combined modulations (Experiment 2) identified a V-shaped response amplitude with a minimum between 135° and 180°. The pupil response phases increased as cone modulation phase increased. The pupil amplitude increased with increasing light level for cone and combined in-phase rod and cone modulation, but not for the rod modulation. Conclusions: These results demonstrate that cone- and rod-pathway contributions are more predominant than melanopsin contribution to the phasic pupil response. The combined rod, cone and melanopsin inputs to the phasic state of the pupil light reflex follow linear summation.
Fil: Barrionuevo, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucuman. Instituto de Investigacion En Luz, Ambiente y Vision; Argentina. University Of Illinois; Estados Unidos
Fil: Nicandro, Nathaniel. University Of Illinois; Estados Unidos
Fil: McAnany, J. Jason. University Of Illinois; Estados Unidos
Fil: Zele, Andrew J.. University Of Queensland; Australia
Fil: Gamlin, Paul. University Of Alabama At Birmingahm; Estados Unidos
Fil: Cao, Dingcai. University Of Illinois; Estados Unidos
Materia
Melanopsin
Flicker
Silent-Substitution
Pupil Light Reflex
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12007
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/12007
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker ResponsesBarrionuevo, Pablo AlejandroNicandro, NathanielMcAnany, J. JasonZele, Andrew J.Gamlin, PaulCao, DingcaiMelanopsinFlickerSilent-SubstitutionPupil Light Reflexhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Purpose: To determine the relative contributions of rods, cones and melanopsin to pupil responses in humans using temporal sinusoidal stimulation for light levels spanning the low mesopic to photopic range. Methods: A four-primary Ganzfeld photostimulator controlled flicker stimulations at seven light levels (-2.7 to 2 log cd/m2) and five frequencies (0.5 to 8Hz). Pupil diameter was measured using a high-resolution eyetracker. Three kinds of sinusoidal photoreceptor modulations were generated using silent substitution: 1) rod modulation, 2) cone modulation, and 3) combined rod and cone modulation in phase (Experiment 1) or phase shifted (Experiment 2) from a fixed rod phase. The melanopsin excitation was computed for each condition. A vector sum model was used to estimate the relative contribution of rods, cones and melanopsin to the pupil response. Results: From Experiment 1, the pupil frequency response peaked at 1Hz at two mesopic light levels for the three modulation conditions. Analyzing the rod-cone phase difference for the combined modulations (Experiment 2) identified a V-shaped response amplitude with a minimum between 135° and 180°. The pupil response phases increased as cone modulation phase increased. The pupil amplitude increased with increasing light level for cone and combined in-phase rod and cone modulation, but not for the rod modulation. Conclusions: These results demonstrate that cone- and rod-pathway contributions are more predominant than melanopsin contribution to the phasic pupil response. The combined rod, cone and melanopsin inputs to the phasic state of the pupil light reflex follow linear summation.Fil: Barrionuevo, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucuman. Instituto de Investigacion En Luz, Ambiente y Vision; Argentina. University Of Illinois; Estados UnidosFil: Nicandro, Nathaniel. University Of Illinois; Estados UnidosFil: McAnany, J. Jason. University Of Illinois; Estados UnidosFil: Zele, Andrew J.. University Of Queensland; AustraliaFil: Gamlin, Paul. University Of Alabama At Birmingahm; Estados UnidosFil: Cao, Dingcai. University Of Illinois; Estados UnidosAssociation for Research in Vision and Ophthalmology2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12007Barrionuevo, Pablo Alejandro; Nicandro, Nathaniel; McAnany, J. Jason; Zele, Andrew J.; Gamlin, Paul; et al.; Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 55; 2; 2-2014; 719-7270146-04041552-5783enginfo:eu-repo/semantics/altIdentifier/url/http://iovs.arvojournals.org/article.aspx?articleid=2189826info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.13-13252info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3915766/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:00Zoai:ri.conicet.gov.ar:11336/12007instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:00.399CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses
title Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses
spellingShingle Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses
Barrionuevo, Pablo Alejandro
Melanopsin
Flicker
Silent-Substitution
Pupil Light Reflex
title_short Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses
title_full Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses
title_fullStr Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses
title_full_unstemmed Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses
title_sort Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses
dc.creator.none.fl_str_mv Barrionuevo, Pablo Alejandro
Nicandro, Nathaniel
McAnany, J. Jason
Zele, Andrew J.
Gamlin, Paul
Cao, Dingcai
author Barrionuevo, Pablo Alejandro
author_facet Barrionuevo, Pablo Alejandro
Nicandro, Nathaniel
McAnany, J. Jason
Zele, Andrew J.
Gamlin, Paul
Cao, Dingcai
author_role author
author2 Nicandro, Nathaniel
McAnany, J. Jason
Zele, Andrew J.
Gamlin, Paul
Cao, Dingcai
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Melanopsin
Flicker
Silent-Substitution
Pupil Light Reflex
topic Melanopsin
Flicker
Silent-Substitution
Pupil Light Reflex
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Purpose: To determine the relative contributions of rods, cones and melanopsin to pupil responses in humans using temporal sinusoidal stimulation for light levels spanning the low mesopic to photopic range. Methods: A four-primary Ganzfeld photostimulator controlled flicker stimulations at seven light levels (-2.7 to 2 log cd/m2) and five frequencies (0.5 to 8Hz). Pupil diameter was measured using a high-resolution eyetracker. Three kinds of sinusoidal photoreceptor modulations were generated using silent substitution: 1) rod modulation, 2) cone modulation, and 3) combined rod and cone modulation in phase (Experiment 1) or phase shifted (Experiment 2) from a fixed rod phase. The melanopsin excitation was computed for each condition. A vector sum model was used to estimate the relative contribution of rods, cones and melanopsin to the pupil response. Results: From Experiment 1, the pupil frequency response peaked at 1Hz at two mesopic light levels for the three modulation conditions. Analyzing the rod-cone phase difference for the combined modulations (Experiment 2) identified a V-shaped response amplitude with a minimum between 135° and 180°. The pupil response phases increased as cone modulation phase increased. The pupil amplitude increased with increasing light level for cone and combined in-phase rod and cone modulation, but not for the rod modulation. Conclusions: These results demonstrate that cone- and rod-pathway contributions are more predominant than melanopsin contribution to the phasic pupil response. The combined rod, cone and melanopsin inputs to the phasic state of the pupil light reflex follow linear summation.
Fil: Barrionuevo, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucuman. Instituto de Investigacion En Luz, Ambiente y Vision; Argentina. University Of Illinois; Estados Unidos
Fil: Nicandro, Nathaniel. University Of Illinois; Estados Unidos
Fil: McAnany, J. Jason. University Of Illinois; Estados Unidos
Fil: Zele, Andrew J.. University Of Queensland; Australia
Fil: Gamlin, Paul. University Of Alabama At Birmingahm; Estados Unidos
Fil: Cao, Dingcai. University Of Illinois; Estados Unidos
description Purpose: To determine the relative contributions of rods, cones and melanopsin to pupil responses in humans using temporal sinusoidal stimulation for light levels spanning the low mesopic to photopic range. Methods: A four-primary Ganzfeld photostimulator controlled flicker stimulations at seven light levels (-2.7 to 2 log cd/m2) and five frequencies (0.5 to 8Hz). Pupil diameter was measured using a high-resolution eyetracker. Three kinds of sinusoidal photoreceptor modulations were generated using silent substitution: 1) rod modulation, 2) cone modulation, and 3) combined rod and cone modulation in phase (Experiment 1) or phase shifted (Experiment 2) from a fixed rod phase. The melanopsin excitation was computed for each condition. A vector sum model was used to estimate the relative contribution of rods, cones and melanopsin to the pupil response. Results: From Experiment 1, the pupil frequency response peaked at 1Hz at two mesopic light levels for the three modulation conditions. Analyzing the rod-cone phase difference for the combined modulations (Experiment 2) identified a V-shaped response amplitude with a minimum between 135° and 180°. The pupil response phases increased as cone modulation phase increased. The pupil amplitude increased with increasing light level for cone and combined in-phase rod and cone modulation, but not for the rod modulation. Conclusions: These results demonstrate that cone- and rod-pathway contributions are more predominant than melanopsin contribution to the phasic pupil response. The combined rod, cone and melanopsin inputs to the phasic state of the pupil light reflex follow linear summation.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12007
Barrionuevo, Pablo Alejandro; Nicandro, Nathaniel; McAnany, J. Jason; Zele, Andrew J.; Gamlin, Paul; et al.; Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 55; 2; 2-2014; 719-727
0146-0404
1552-5783
url http://hdl.handle.net/11336/12007
identifier_str_mv Barrionuevo, Pablo Alejandro; Nicandro, Nathaniel; McAnany, J. Jason; Zele, Andrew J.; Gamlin, Paul; et al.; Assessing Rod, Cone, and Melanopsin Contributions to Human Pupil Flicker Responses; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 55; 2; 2-2014; 719-727
0146-0404
1552-5783
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://iovs.arvojournals.org/article.aspx?articleid=2189826
info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.13-13252
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3915766/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082856667840512
score 13.22299

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