Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris

Autores
Rípodas, Carolina; Dalla Via, Maria Virginia; Aguilar, Orlando Mario; Zanetti, María Eugenia; Blanco, Flavio Antonio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Flavonoids and isoflavonoids participate in the signaling exchange between roots of legumes and nitrogen-fixing rhizobia and can promote division of cortical cells during nodule formation by inhibiting auxin transport. Here, we report the characterization of a member of the common bean isoflavone reductase (EC 1.3.1.45, PvIFR1) gene family, an enzyme that participates in the last steps of the biosynthetic pathway of isoflavonoids. Transcript levels of PvIFR1 were detected preferentially in the susceptible zone of roots, augmented upon nitrogen starvation and in response to Rhizobium etli inoculation at very early stages of the interaction. Knockdown of PvIFR1 mediated by RNA interference (RNAi) in common bean composite plants resulted in a reduction of shoot and root length. Furthermore, reduction of PvIFR1 mRNAs also affected growth of lateral roots after emergence, a stage in which auxins are required to establish a persistent meristem. Upon inoculation, the number of nodules formed by different strains of R. etli was significantly lower in IFR RNAi than in control roots. Transcript levels of two auxinregulated genes are consistent with lower levels of auxin in PvIFR1 silenced roots. These results suggest a complex role of PvIFR1 during plant growth, root development and symbiosis, all processes in which auxin transport is involved.
Fil: Rípodas, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Dalla Via, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
Isoflavonoids
Lateral Root
Rhizobium
Nitrogen Fixation
Legume
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23941
Acceder
id CONICETDig_6de2de5b12346daad8595588ee42c4cb
oai_identifier_str oai:ri.conicet.gov.ar:11336/23941
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgarisRípodas, CarolinaDalla Via, Maria VirginiaAguilar, Orlando MarioZanetti, María EugeniaBlanco, Flavio AntonioIsoflavonoidsLateral RootRhizobiumNitrogen FixationLegumehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flavonoids and isoflavonoids participate in the signaling exchange between roots of legumes and nitrogen-fixing rhizobia and can promote division of cortical cells during nodule formation by inhibiting auxin transport. Here, we report the characterization of a member of the common bean isoflavone reductase (EC 1.3.1.45, PvIFR1) gene family, an enzyme that participates in the last steps of the biosynthetic pathway of isoflavonoids. Transcript levels of PvIFR1 were detected preferentially in the susceptible zone of roots, augmented upon nitrogen starvation and in response to Rhizobium etli inoculation at very early stages of the interaction. Knockdown of PvIFR1 mediated by RNA interference (RNAi) in common bean composite plants resulted in a reduction of shoot and root length. Furthermore, reduction of PvIFR1 mRNAs also affected growth of lateral roots after emergence, a stage in which auxins are required to establish a persistent meristem. Upon inoculation, the number of nodules formed by different strains of R. etli was significantly lower in IFR RNAi than in control roots. Transcript levels of two auxinregulated genes are consistent with lower levels of auxin in PvIFR1 silenced roots. These results suggest a complex role of PvIFR1 during plant growth, root development and symbiosis, all processes in which auxin transport is involved.Fil: Rípodas, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Dalla Via, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaElsevier France-editions Scientifiques Medicales Elsevier2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23941Rípodas, Carolina; Dalla Via, Maria Virginia; Aguilar, Orlando Mario; Zanetti, María Eugenia; Blanco, Flavio Antonio; Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 68; 7-2013; 1-90981-9428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942813001290info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2013.04.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:19:22Zoai:ri.conicet.gov.ar:11336/23941instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:19:23.208CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris
title Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris
spellingShingle Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris
Rípodas, Carolina
Isoflavonoids
Lateral Root
Rhizobium
Nitrogen Fixation
Legume
title_short Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris
title_full Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris
title_fullStr Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris
title_full_unstemmed Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris
title_sort Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris
dc.creator.none.fl_str_mv Rípodas, Carolina
Dalla Via, Maria Virginia
Aguilar, Orlando Mario
Zanetti, María Eugenia
Blanco, Flavio Antonio
author Rípodas, Carolina
author_facet Rípodas, Carolina
Dalla Via, Maria Virginia
Aguilar, Orlando Mario
Zanetti, María Eugenia
Blanco, Flavio Antonio
author_role author
author2 Dalla Via, Maria Virginia
Aguilar, Orlando Mario
Zanetti, María Eugenia
Blanco, Flavio Antonio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Isoflavonoids
Lateral Root
Rhizobium
Nitrogen Fixation
Legume
topic Isoflavonoids
Lateral Root
Rhizobium
Nitrogen Fixation
Legume
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Flavonoids and isoflavonoids participate in the signaling exchange between roots of legumes and nitrogen-fixing rhizobia and can promote division of cortical cells during nodule formation by inhibiting auxin transport. Here, we report the characterization of a member of the common bean isoflavone reductase (EC 1.3.1.45, PvIFR1) gene family, an enzyme that participates in the last steps of the biosynthetic pathway of isoflavonoids. Transcript levels of PvIFR1 were detected preferentially in the susceptible zone of roots, augmented upon nitrogen starvation and in response to Rhizobium etli inoculation at very early stages of the interaction. Knockdown of PvIFR1 mediated by RNA interference (RNAi) in common bean composite plants resulted in a reduction of shoot and root length. Furthermore, reduction of PvIFR1 mRNAs also affected growth of lateral roots after emergence, a stage in which auxins are required to establish a persistent meristem. Upon inoculation, the number of nodules formed by different strains of R. etli was significantly lower in IFR RNAi than in control roots. Transcript levels of two auxinregulated genes are consistent with lower levels of auxin in PvIFR1 silenced roots. These results suggest a complex role of PvIFR1 during plant growth, root development and symbiosis, all processes in which auxin transport is involved.
Fil: Rípodas, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Dalla Via, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Flavonoids and isoflavonoids participate in the signaling exchange between roots of legumes and nitrogen-fixing rhizobia and can promote division of cortical cells during nodule formation by inhibiting auxin transport. Here, we report the characterization of a member of the common bean isoflavone reductase (EC 1.3.1.45, PvIFR1) gene family, an enzyme that participates in the last steps of the biosynthetic pathway of isoflavonoids. Transcript levels of PvIFR1 were detected preferentially in the susceptible zone of roots, augmented upon nitrogen starvation and in response to Rhizobium etli inoculation at very early stages of the interaction. Knockdown of PvIFR1 mediated by RNA interference (RNAi) in common bean composite plants resulted in a reduction of shoot and root length. Furthermore, reduction of PvIFR1 mRNAs also affected growth of lateral roots after emergence, a stage in which auxins are required to establish a persistent meristem. Upon inoculation, the number of nodules formed by different strains of R. etli was significantly lower in IFR RNAi than in control roots. Transcript levels of two auxinregulated genes are consistent with lower levels of auxin in PvIFR1 silenced roots. These results suggest a complex role of PvIFR1 during plant growth, root development and symbiosis, all processes in which auxin transport is involved.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23941
Rípodas, Carolina; Dalla Via, Maria Virginia; Aguilar, Orlando Mario; Zanetti, María Eugenia; Blanco, Flavio Antonio; Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 68; 7-2013; 1-9
0981-9428
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23941
identifier_str_mv Rípodas, Carolina; Dalla Via, Maria Virginia; Aguilar, Orlando Mario; Zanetti, María Eugenia; Blanco, Flavio Antonio; Knock-down of a member of the isoflavone reductase gene family impairs plant growth and nodulation in Phaseolus vulgaris; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 68; 7-2013; 1-9
0981-9428
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942813001290
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2013.04.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier France-editions Scientifiques Medicales Elsevier
publisher.none.fl_str_mv Elsevier France-editions Scientifiques Medicales Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781669980241920
score 12.928904

Publicaciones similares