Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation

Autores
Ibarguren, Carolina; Grosso, Carlos; Apella, Maria Cristina; Audisio, Marcela Carina
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The encapsulation of enterocins synthesized by Enterococcus faecium CRL1385 through ionic gelation with calcium ions was analyzed. Different enterocins samples were lyophilised and encapsulated using low-methoxyl pectin as the coating material. Lipids present in milk butter were also added to control the release of antimicrobial peptides from the capsules. The morphology of fresh and freeze-dried capsules was examined using light microscopy and scanning electron microscopy, respectively. Antimicrobial activity of encapsulated bacteriocins was assessed against Listeria monocytogenes 01/155 using the agar diffusion technique and direct contact in microplates. The capsules with higher lipid content showed a more spherical and uniform shape. Pathogen inhibition was observed for capsules prepared with different bacteriocin solutions both on solid (halo diameter ¼ 8.5e13.5 mm) and in an aqueous medium (ca. 2 log orders decline in L. monocytogenes viability). The outcomes suggest that bacteriocin encapsulation through ionic gelation can be a potential alternative for the application of these antimicrobial peptides as biopreservatives in food.
Fil: Ibarguren, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina. Universidad Nacional de Salta; Argentina
Fil: Grosso, Carlos. Universidade Estadual de Campinas; Brasil
Fil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina. Universidad Nacional de Salta; Argentina
Materia
Enterocin
Ionic Gelation
Listeria Monocytogenes
Food Biopreservation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14215
Acceder
id CONICETDig_16a2b15f9b6448a052e663aea6005967
oai_identifier_str oai:ri.conicet.gov.ar:11336/14215
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelationIbarguren, CarolinaGrosso, CarlosApella, Maria CristinaAudisio, Marcela CarinaEnterocinIonic GelationListeria MonocytogenesFood Biopreservationhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The encapsulation of enterocins synthesized by Enterococcus faecium CRL1385 through ionic gelation with calcium ions was analyzed. Different enterocins samples were lyophilised and encapsulated using low-methoxyl pectin as the coating material. Lipids present in milk butter were also added to control the release of antimicrobial peptides from the capsules. The morphology of fresh and freeze-dried capsules was examined using light microscopy and scanning electron microscopy, respectively. Antimicrobial activity of encapsulated bacteriocins was assessed against Listeria monocytogenes 01/155 using the agar diffusion technique and direct contact in microplates. The capsules with higher lipid content showed a more spherical and uniform shape. Pathogen inhibition was observed for capsules prepared with different bacteriocin solutions both on solid (halo diameter ¼ 8.5e13.5 mm) and in an aqueous medium (ca. 2 log orders decline in L. monocytogenes viability). The outcomes suggest that bacteriocin encapsulation through ionic gelation can be a potential alternative for the application of these antimicrobial peptides as biopreservatives in food.Fil: Ibarguren, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina. Universidad Nacional de Salta; ArgentinaFil: Grosso, Carlos. Universidade Estadual de Campinas; BrasilFil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina. Universidad Nacional de Salta; ArgentinaElsevier2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14215Ibarguren, Carolina; Grosso, Carlos; Apella, Maria Cristina; Audisio, Marcela Carina; Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation; Elsevier; Food Hydrocolloids; 29; 1; 10-2012; 21-260268-005Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodhyd.2012.01.017info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0268005X1200029Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:08Zoai:ri.conicet.gov.ar:11336/14215instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:08.451CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation
title Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation
spellingShingle Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation
Ibarguren, Carolina
Enterocin
Ionic Gelation
Listeria Monocytogenes
Food Biopreservation
title_short Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation
title_full Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation
title_fullStr Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation
title_full_unstemmed Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation
title_sort Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation
dc.creator.none.fl_str_mv Ibarguren, Carolina
Grosso, Carlos
Apella, Maria Cristina
Audisio, Marcela Carina
author Ibarguren, Carolina
author_facet Ibarguren, Carolina
Grosso, Carlos
Apella, Maria Cristina
Audisio, Marcela Carina
author_role author
author2 Grosso, Carlos
Apella, Maria Cristina
Audisio, Marcela Carina
author2_role author
author
author
dc.subject.none.fl_str_mv Enterocin
Ionic Gelation
Listeria Monocytogenes
Food Biopreservation
topic Enterocin
Ionic Gelation
Listeria Monocytogenes
Food Biopreservation
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The encapsulation of enterocins synthesized by Enterococcus faecium CRL1385 through ionic gelation with calcium ions was analyzed. Different enterocins samples were lyophilised and encapsulated using low-methoxyl pectin as the coating material. Lipids present in milk butter were also added to control the release of antimicrobial peptides from the capsules. The morphology of fresh and freeze-dried capsules was examined using light microscopy and scanning electron microscopy, respectively. Antimicrobial activity of encapsulated bacteriocins was assessed against Listeria monocytogenes 01/155 using the agar diffusion technique and direct contact in microplates. The capsules with higher lipid content showed a more spherical and uniform shape. Pathogen inhibition was observed for capsules prepared with different bacteriocin solutions both on solid (halo diameter ¼ 8.5e13.5 mm) and in an aqueous medium (ca. 2 log orders decline in L. monocytogenes viability). The outcomes suggest that bacteriocin encapsulation through ionic gelation can be a potential alternative for the application of these antimicrobial peptides as biopreservatives in food.
Fil: Ibarguren, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina. Universidad Nacional de Salta; Argentina
Fil: Grosso, Carlos. Universidade Estadual de Campinas; Brasil
Fil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina. Universidad Nacional de Salta; Argentina
description The encapsulation of enterocins synthesized by Enterococcus faecium CRL1385 through ionic gelation with calcium ions was analyzed. Different enterocins samples were lyophilised and encapsulated using low-methoxyl pectin as the coating material. Lipids present in milk butter were also added to control the release of antimicrobial peptides from the capsules. The morphology of fresh and freeze-dried capsules was examined using light microscopy and scanning electron microscopy, respectively. Antimicrobial activity of encapsulated bacteriocins was assessed against Listeria monocytogenes 01/155 using the agar diffusion technique and direct contact in microplates. The capsules with higher lipid content showed a more spherical and uniform shape. Pathogen inhibition was observed for capsules prepared with different bacteriocin solutions both on solid (halo diameter ¼ 8.5e13.5 mm) and in an aqueous medium (ca. 2 log orders decline in L. monocytogenes viability). The outcomes suggest that bacteriocin encapsulation through ionic gelation can be a potential alternative for the application of these antimicrobial peptides as biopreservatives in food.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14215
Ibarguren, Carolina; Grosso, Carlos; Apella, Maria Cristina; Audisio, Marcela Carina; Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation; Elsevier; Food Hydrocolloids; 29; 1; 10-2012; 21-26
0268-005X
url http://hdl.handle.net/11336/14215
identifier_str_mv Ibarguren, Carolina; Grosso, Carlos; Apella, Maria Cristina; Audisio, Marcela Carina; Anti-Listeria monocytogenes activity of enterocins microencapsulated by ionic gelation; Elsevier; Food Hydrocolloids; 29; 1; 10-2012; 21-26
0268-005X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodhyd.2012.01.017
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0268005X1200029X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269326378270720
score 13.13397

Publicaciones similares