Role of nitric oxide in soybean cotyledon senescence

Autores
Jasid, Sebastian Alberto; Galatro, Andrea Verónica; Villordo, Juan Javier; Puntarulo, Susana Ángela; Simontacchi, Marcela Silvia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Senescence was studied in soybean (Glycine max, var ALM 4500) cotyledons from 10- and 25-day-old seedlings. Rejuvenated cotyledons, obtained by removing epicotyls from 5-day seedlings, showed a lower decrease in the content of photosynthetic pigments from day 10 to day 25, as compared to naturally senescent cotyledons. Unlike controls, rejuvenated cotyledons showed no changes in electrolyte leakage, chlorophyll a, and carotene content during the studied period. At day 10, nitric oxide (NO) content in rejuvenated cotyledons was higher than in naturally senescent cotyledons. The role of NO in senescence was assessed by exposing seedlings to sodium nitroprusside (SNP). SNP-treated cotyledons showed a constant NO content over the studied period of 4.0 0.4 nmol g1 FW, conserved photosynthetic pigment content and lower lipid radical content, as compared to naturally senescent cotyledons. These results suggest that NO can protect against senescence-dependent alterations in cotyledons, because (i) exogenous NO can partially protect naturally senescent cotyledons and (ii) rejuvenated cotyledons, which do not show the senescent phenotype, have higher endogenous levels of NO.
Fil: Jasid, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Villordo, Juan Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Simontacchi, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
SOYBEAN COTYLEDONS
LIPID PEROXIDATION
NITRIC OXIDE
PROTEIN OXIDATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102727
Acceder
id CONICETDig_15fbc81347ec9907cbdd09d5f9fafb94
oai_identifier_str oai:ri.conicet.gov.ar:11336/102727
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of nitric oxide in soybean cotyledon senescenceJasid, Sebastian AlbertoGalatro, Andrea VerónicaVillordo, Juan JavierPuntarulo, Susana ÁngelaSimontacchi, Marcela SilviaSOYBEAN COTYLEDONSLIPID PEROXIDATIONNITRIC OXIDEPROTEIN OXIDATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Senescence was studied in soybean (Glycine max, var ALM 4500) cotyledons from 10- and 25-day-old seedlings. Rejuvenated cotyledons, obtained by removing epicotyls from 5-day seedlings, showed a lower decrease in the content of photosynthetic pigments from day 10 to day 25, as compared to naturally senescent cotyledons. Unlike controls, rejuvenated cotyledons showed no changes in electrolyte leakage, chlorophyll a, and carotene content during the studied period. At day 10, nitric oxide (NO) content in rejuvenated cotyledons was higher than in naturally senescent cotyledons. The role of NO in senescence was assessed by exposing seedlings to sodium nitroprusside (SNP). SNP-treated cotyledons showed a constant NO content over the studied period of 4.0 0.4 nmol g1 FW, conserved photosynthetic pigment content and lower lipid radical content, as compared to naturally senescent cotyledons. These results suggest that NO can protect against senescence-dependent alterations in cotyledons, because (i) exogenous NO can partially protect naturally senescent cotyledons and (ii) rejuvenated cotyledons, which do not show the senescent phenotype, have higher endogenous levels of NO.Fil: Jasid, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Villordo, Juan Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Simontacchi, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaElsevier Ireland2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102727Jasid, Sebastian Alberto; Galatro, Andrea Verónica; Villordo, Juan Javier; Puntarulo, Susana Ángela; Simontacchi, Marcela Silvia; Role of nitric oxide in soybean cotyledon senescence; Elsevier Ireland; Plant Science; 176; 5; 5-2009; 662-6680168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2009.02.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945209000405info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:56Zoai:ri.conicet.gov.ar:11336/102727instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:56.263CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of nitric oxide in soybean cotyledon senescence
title Role of nitric oxide in soybean cotyledon senescence
spellingShingle Role of nitric oxide in soybean cotyledon senescence
Jasid, Sebastian Alberto
SOYBEAN COTYLEDONS
LIPID PEROXIDATION
NITRIC OXIDE
PROTEIN OXIDATION
title_short Role of nitric oxide in soybean cotyledon senescence
title_full Role of nitric oxide in soybean cotyledon senescence
title_fullStr Role of nitric oxide in soybean cotyledon senescence
title_full_unstemmed Role of nitric oxide in soybean cotyledon senescence
title_sort Role of nitric oxide in soybean cotyledon senescence
dc.creator.none.fl_str_mv Jasid, Sebastian Alberto
Galatro, Andrea Verónica
Villordo, Juan Javier
Puntarulo, Susana Ángela
Simontacchi, Marcela Silvia
author Jasid, Sebastian Alberto
author_facet Jasid, Sebastian Alberto
Galatro, Andrea Verónica
Villordo, Juan Javier
Puntarulo, Susana Ángela
Simontacchi, Marcela Silvia
author_role author
author2 Galatro, Andrea Verónica
Villordo, Juan Javier
Puntarulo, Susana Ángela
Simontacchi, Marcela Silvia
author2_role author
author
author
author
dc.subject.none.fl_str_mv SOYBEAN COTYLEDONS
LIPID PEROXIDATION
NITRIC OXIDE
PROTEIN OXIDATION
topic SOYBEAN COTYLEDONS
LIPID PEROXIDATION
NITRIC OXIDE
PROTEIN OXIDATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Senescence was studied in soybean (Glycine max, var ALM 4500) cotyledons from 10- and 25-day-old seedlings. Rejuvenated cotyledons, obtained by removing epicotyls from 5-day seedlings, showed a lower decrease in the content of photosynthetic pigments from day 10 to day 25, as compared to naturally senescent cotyledons. Unlike controls, rejuvenated cotyledons showed no changes in electrolyte leakage, chlorophyll a, and carotene content during the studied period. At day 10, nitric oxide (NO) content in rejuvenated cotyledons was higher than in naturally senescent cotyledons. The role of NO in senescence was assessed by exposing seedlings to sodium nitroprusside (SNP). SNP-treated cotyledons showed a constant NO content over the studied period of 4.0 0.4 nmol g1 FW, conserved photosynthetic pigment content and lower lipid radical content, as compared to naturally senescent cotyledons. These results suggest that NO can protect against senescence-dependent alterations in cotyledons, because (i) exogenous NO can partially protect naturally senescent cotyledons and (ii) rejuvenated cotyledons, which do not show the senescent phenotype, have higher endogenous levels of NO.
Fil: Jasid, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Villordo, Juan Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Simontacchi, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Senescence was studied in soybean (Glycine max, var ALM 4500) cotyledons from 10- and 25-day-old seedlings. Rejuvenated cotyledons, obtained by removing epicotyls from 5-day seedlings, showed a lower decrease in the content of photosynthetic pigments from day 10 to day 25, as compared to naturally senescent cotyledons. Unlike controls, rejuvenated cotyledons showed no changes in electrolyte leakage, chlorophyll a, and carotene content during the studied period. At day 10, nitric oxide (NO) content in rejuvenated cotyledons was higher than in naturally senescent cotyledons. The role of NO in senescence was assessed by exposing seedlings to sodium nitroprusside (SNP). SNP-treated cotyledons showed a constant NO content over the studied period of 4.0 0.4 nmol g1 FW, conserved photosynthetic pigment content and lower lipid radical content, as compared to naturally senescent cotyledons. These results suggest that NO can protect against senescence-dependent alterations in cotyledons, because (i) exogenous NO can partially protect naturally senescent cotyledons and (ii) rejuvenated cotyledons, which do not show the senescent phenotype, have higher endogenous levels of NO.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102727
Jasid, Sebastian Alberto; Galatro, Andrea Verónica; Villordo, Juan Javier; Puntarulo, Susana Ángela; Simontacchi, Marcela Silvia; Role of nitric oxide in soybean cotyledon senescence; Elsevier Ireland; Plant Science; 176; 5; 5-2009; 662-668
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102727
identifier_str_mv Jasid, Sebastian Alberto; Galatro, Andrea Verónica; Villordo, Juan Javier; Puntarulo, Susana Ángela; Simontacchi, Marcela Silvia; Role of nitric oxide in soybean cotyledon senescence; Elsevier Ireland; Plant Science; 176; 5; 5-2009; 662-668
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2009.02.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945209000405
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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