Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons

Autores
Galatro, Andrea Verónica; Puntarulo, Susana Ángela; Guiamet, Juan José; Simontacchi, Marcela Silvia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The subcellular localization of NO generation in soybean cotyledons, and the relationship between NO synthesis and in vivo chloroplast performance were studied. Employing the NO probe 4-aminomethyl-2′,7′-difluorofluorescein diacetate (DAF-FM DA) and fluorescence microscopy, a strongly punctuated fluorescence was detected in mesophyll cells. The co-localization of DAF-FM and chlorophyll fluorescence, in confocal laser microscopy images, indicated the presence of NO in the chloroplasts. NO visualization was dependent on light, seedling age, and chloroplast function throughout cotyledons lifespan. The addition of herbicides with action in chloroplasts (DCMU and paraquat) dramatically reduced the quantum yield of photosystem II (ϕPSII), and lead to images with absence of punctuated green fluorescence. Moreover, electron paramagnetic resonance signals corresponding to NO-spin trap adduct observed in cotyledon homogenates decreased significantly by the treatment with herbicides, as compared to controls. Neither chloroplast function nor NO content were significantly different in cotyledons from plants growing in the presence of ammonium or nitrate as the nitrogen source. These findings suggest that chloroplasts are organelles that contribute to NO synthesis in vivo, and that their proper functionality is essential for maintaining NO levels in soybean cotyledons.
Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Guiamet, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Simontacchi, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Materia
Nitric Oxide
Soybean Cotyledons
Chloroplasts
Epr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21284
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/21284
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledonsGalatro, Andrea VerónicaPuntarulo, Susana ÁngelaGuiamet, Juan JoséSimontacchi, Marcela SilviaNitric OxideSoybean CotyledonsChloroplastsEprhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The subcellular localization of NO generation in soybean cotyledons, and the relationship between NO synthesis and in vivo chloroplast performance were studied. Employing the NO probe 4-aminomethyl-2′,7′-difluorofluorescein diacetate (DAF-FM DA) and fluorescence microscopy, a strongly punctuated fluorescence was detected in mesophyll cells. The co-localization of DAF-FM and chlorophyll fluorescence, in confocal laser microscopy images, indicated the presence of NO in the chloroplasts. NO visualization was dependent on light, seedling age, and chloroplast function throughout cotyledons lifespan. The addition of herbicides with action in chloroplasts (DCMU and paraquat) dramatically reduced the quantum yield of photosystem II (ϕPSII), and lead to images with absence of punctuated green fluorescence. Moreover, electron paramagnetic resonance signals corresponding to NO-spin trap adduct observed in cotyledon homogenates decreased significantly by the treatment with herbicides, as compared to controls. Neither chloroplast function nor NO content were significantly different in cotyledons from plants growing in the presence of ammonium or nitrate as the nitrogen source. These findings suggest that chloroplasts are organelles that contribute to NO synthesis in vivo, and that their proper functionality is essential for maintaining NO levels in soybean cotyledons.Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Guiamet, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Simontacchi, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaElsevier Masson2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21284Galatro, Andrea Verónica; Puntarulo, Susana Ángela; Guiamet, Juan José; Simontacchi, Marcela Silvia; Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons; Elsevier Masson; Plant Physiology and Biochemistry; 66; 5-2013; 26-330981-9428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2013.01.019info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942813000405info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:43Zoai:ri.conicet.gov.ar:11336/21284instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:43.719CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons
title Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons
spellingShingle Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons
Galatro, Andrea Verónica
Nitric Oxide
Soybean Cotyledons
Chloroplasts
Epr
title_short Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons
title_full Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons
title_fullStr Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons
title_full_unstemmed Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons
title_sort Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons
dc.creator.none.fl_str_mv Galatro, Andrea Verónica
Puntarulo, Susana Ángela
Guiamet, Juan José
Simontacchi, Marcela Silvia
author Galatro, Andrea Verónica
author_facet Galatro, Andrea Verónica
Puntarulo, Susana Ángela
Guiamet, Juan José
Simontacchi, Marcela Silvia
author_role author
author2 Puntarulo, Susana Ángela
Guiamet, Juan José
Simontacchi, Marcela Silvia
author2_role author
author
author
dc.subject.none.fl_str_mv Nitric Oxide
Soybean Cotyledons
Chloroplasts
Epr
topic Nitric Oxide
Soybean Cotyledons
Chloroplasts
Epr
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The subcellular localization of NO generation in soybean cotyledons, and the relationship between NO synthesis and in vivo chloroplast performance were studied. Employing the NO probe 4-aminomethyl-2′,7′-difluorofluorescein diacetate (DAF-FM DA) and fluorescence microscopy, a strongly punctuated fluorescence was detected in mesophyll cells. The co-localization of DAF-FM and chlorophyll fluorescence, in confocal laser microscopy images, indicated the presence of NO in the chloroplasts. NO visualization was dependent on light, seedling age, and chloroplast function throughout cotyledons lifespan. The addition of herbicides with action in chloroplasts (DCMU and paraquat) dramatically reduced the quantum yield of photosystem II (ϕPSII), and lead to images with absence of punctuated green fluorescence. Moreover, electron paramagnetic resonance signals corresponding to NO-spin trap adduct observed in cotyledon homogenates decreased significantly by the treatment with herbicides, as compared to controls. Neither chloroplast function nor NO content were significantly different in cotyledons from plants growing in the presence of ammonium or nitrate as the nitrogen source. These findings suggest that chloroplasts are organelles that contribute to NO synthesis in vivo, and that their proper functionality is essential for maintaining NO levels in soybean cotyledons.
Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Guiamet, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Simontacchi, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
description The subcellular localization of NO generation in soybean cotyledons, and the relationship between NO synthesis and in vivo chloroplast performance were studied. Employing the NO probe 4-aminomethyl-2′,7′-difluorofluorescein diacetate (DAF-FM DA) and fluorescence microscopy, a strongly punctuated fluorescence was detected in mesophyll cells. The co-localization of DAF-FM and chlorophyll fluorescence, in confocal laser microscopy images, indicated the presence of NO in the chloroplasts. NO visualization was dependent on light, seedling age, and chloroplast function throughout cotyledons lifespan. The addition of herbicides with action in chloroplasts (DCMU and paraquat) dramatically reduced the quantum yield of photosystem II (ϕPSII), and lead to images with absence of punctuated green fluorescence. Moreover, electron paramagnetic resonance signals corresponding to NO-spin trap adduct observed in cotyledon homogenates decreased significantly by the treatment with herbicides, as compared to controls. Neither chloroplast function nor NO content were significantly different in cotyledons from plants growing in the presence of ammonium or nitrate as the nitrogen source. These findings suggest that chloroplasts are organelles that contribute to NO synthesis in vivo, and that their proper functionality is essential for maintaining NO levels in soybean cotyledons.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21284
Galatro, Andrea Verónica; Puntarulo, Susana Ángela; Guiamet, Juan José; Simontacchi, Marcela Silvia; Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons; Elsevier Masson; Plant Physiology and Biochemistry; 66; 5-2013; 26-33
0981-9428
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21284
identifier_str_mv Galatro, Andrea Verónica; Puntarulo, Susana Ángela; Guiamet, Juan José; Simontacchi, Marcela Silvia; Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons; Elsevier Masson; Plant Physiology and Biochemistry; 66; 5-2013; 26-33
0981-9428
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2013.01.019
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942813000405
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Masson
publisher.none.fl_str_mv Elsevier Masson
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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