Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy

Autores
Tirado González, Irene; Freitag, Nancy; Barrientos, Gabriela Laura; Shaikly, Valerie; Nagaeva, Olga; Strand, Magnus; Kjellberg, Lennart; Klapp, Burghard F.; Mincheva Nilsson, Lucia; Cohen, Marie; Blois, Sandra M.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Galectin-1 (gal-1) is expressed at the feto-maternal interface and plays a role in regulating the maternal immune response against placental alloantigens, contributing to pregnancy maintenance. Both decidua and placenta contribute to gal-1 expression and may be important for the maternal immune regulation. The expression of gal-1 within the placenta is considered relevant to cell-adhesion and invasion of trophoblasts, but the role of gal-1 in the immune evasion machinery exhibited by trophoblast cells remains to be elucidated. In this study, we analyzed gal-1 expression in preimplantation human embryos and first-trimester decidua-placenta specimens and serum gal-1 levels to investigate the physiological role played by this lectin during pregnancy. The effect on human leukocyte antigen G (HLAG) expression in response to stimulation or silencing of gal-1 was also determined in the human invasive, proliferative extravillous cytotrophoblast 65 (HIPEC65) cell line. Compared with normal pregnant women, circulating gal-1 levels were significantly decreased in patients who subsequently suffered a miscarriage. Human embryos undergoing preimplantation development expressed gal-1 on the trophectoderm and inner cell mass. Furthermore, our in vitro experiments showed that exogenous gal-1 positively regulated the membrane-bound HLA-G isoforms (HLA-G1 and G2) in HIPEC65 cells, whereas endogenous gal-1 also induced expression of the soluble isoforms (HLA-G5 and -G6). Our results suggest that gal-1 plays a key role in pregnancy maternal immune regulation by modulating HLA-G expression on trophoblast cells. Circulating gal-1 levels could serve as a predictive factor for pregnancy success in early human gestation.
Fil: Tirado González, Irene. Medicine University Berlin; Alemania
Fil: Freitag, Nancy. Medicine University Berlin; Alemania
Fil: Barrientos, Gabriela Laura. Medicine University Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Shaikly, Valerie. University Of Essex; Reino Unido. Assisted Reproduction and Gynaecology Centre; Reino Unido
Fil: Nagaeva, Olga. Universidad de Umea; Suecia
Fil: Strand, Magnus. Universidad de Umea; Suecia
Fil: Kjellberg, Lennart. Universidad de Umea; Suecia
Fil: Klapp, Burghard F.. Medicine University Berlin; Alemania
Fil: Mincheva Nilsson, Lucia. Universidad de Umea; Suecia
Fil: Cohen, Marie. Laboratoire d’Hormonologie; Suiza
Fil: Blois, Sandra M.. Medicine University Berlin; Alemania
Materia
Gal-1
Hla-G
Human Embryo
Spontaneous Abortion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15939
Acceder
id CONICETDig_15715cc1c59d57f871f0ba64ef1fce3e
oai_identifier_str oai:ri.conicet.gov.ar:11336/15939
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancyTirado González, IreneFreitag, NancyBarrientos, Gabriela LauraShaikly, ValerieNagaeva, OlgaStrand, MagnusKjellberg, LennartKlapp, Burghard F.Mincheva Nilsson, LuciaCohen, MarieBlois, Sandra M.Gal-1Hla-GHuman EmbryoSpontaneous Abortionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Galectin-1 (gal-1) is expressed at the feto-maternal interface and plays a role in regulating the maternal immune response against placental alloantigens, contributing to pregnancy maintenance. Both decidua and placenta contribute to gal-1 expression and may be important for the maternal immune regulation. The expression of gal-1 within the placenta is considered relevant to cell-adhesion and invasion of trophoblasts, but the role of gal-1 in the immune evasion machinery exhibited by trophoblast cells remains to be elucidated. In this study, we analyzed gal-1 expression in preimplantation human embryos and first-trimester decidua-placenta specimens and serum gal-1 levels to investigate the physiological role played by this lectin during pregnancy. The effect on human leukocyte antigen G (HLAG) expression in response to stimulation or silencing of gal-1 was also determined in the human invasive, proliferative extravillous cytotrophoblast 65 (HIPEC65) cell line. Compared with normal pregnant women, circulating gal-1 levels were significantly decreased in patients who subsequently suffered a miscarriage. Human embryos undergoing preimplantation development expressed gal-1 on the trophectoderm and inner cell mass. Furthermore, our in vitro experiments showed that exogenous gal-1 positively regulated the membrane-bound HLA-G isoforms (HLA-G1 and G2) in HIPEC65 cells, whereas endogenous gal-1 also induced expression of the soluble isoforms (HLA-G5 and -G6). Our results suggest that gal-1 plays a key role in pregnancy maternal immune regulation by modulating HLA-G expression on trophoblast cells. Circulating gal-1 levels could serve as a predictive factor for pregnancy success in early human gestation.Fil: Tirado González, Irene. Medicine University Berlin; AlemaniaFil: Freitag, Nancy. Medicine University Berlin; AlemaniaFil: Barrientos, Gabriela Laura. Medicine University Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Shaikly, Valerie. University Of Essex; Reino Unido. Assisted Reproduction and Gynaecology Centre; Reino UnidoFil: Nagaeva, Olga. Universidad de Umea; SueciaFil: Strand, Magnus. Universidad de Umea; SueciaFil: Kjellberg, Lennart. Universidad de Umea; SueciaFil: Klapp, Burghard F.. Medicine University Berlin; AlemaniaFil: Mincheva Nilsson, Lucia. Universidad de Umea; SueciaFil: Cohen, Marie. Laboratoire d’Hormonologie; SuizaFil: Blois, Sandra M.. Medicine University Berlin; AlemaniaOxford University Press2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15939Tirado González, Irene; Freitag, Nancy; Barrientos, Gabriela Laura; Shaikly, Valerie; Nagaeva, Olga; et al.; Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy; Oxford University Press; Molecular Human Reproduction; 19; 1; 1-2013; 43-531360-9947enginfo:eu-repo/semantics/altIdentifier/doi/10.1093/molehr/gas043info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/molehr/article-lookup/doi/10.1093/molehr/gas043info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:55:49Zoai:ri.conicet.gov.ar:11336/15939instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:55:50.124CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy
title Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy
spellingShingle Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy
Tirado González, Irene
Gal-1
Hla-G
Human Embryo
Spontaneous Abortion
title_short Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy
title_full Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy
title_fullStr Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy
title_full_unstemmed Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy
title_sort Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy
dc.creator.none.fl_str_mv Tirado González, Irene
Freitag, Nancy
Barrientos, Gabriela Laura
Shaikly, Valerie
Nagaeva, Olga
Strand, Magnus
Kjellberg, Lennart
Klapp, Burghard F.
Mincheva Nilsson, Lucia
Cohen, Marie
Blois, Sandra M.
author Tirado González, Irene
author_facet Tirado González, Irene
Freitag, Nancy
Barrientos, Gabriela Laura
Shaikly, Valerie
Nagaeva, Olga
Strand, Magnus
Kjellberg, Lennart
Klapp, Burghard F.
Mincheva Nilsson, Lucia
Cohen, Marie
Blois, Sandra M.
author_role author
author2 Freitag, Nancy
Barrientos, Gabriela Laura
Shaikly, Valerie
Nagaeva, Olga
Strand, Magnus
Kjellberg, Lennart
Klapp, Burghard F.
Mincheva Nilsson, Lucia
Cohen, Marie
Blois, Sandra M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gal-1
Hla-G
Human Embryo
Spontaneous Abortion
topic Gal-1
Hla-G
Human Embryo
Spontaneous Abortion
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Galectin-1 (gal-1) is expressed at the feto-maternal interface and plays a role in regulating the maternal immune response against placental alloantigens, contributing to pregnancy maintenance. Both decidua and placenta contribute to gal-1 expression and may be important for the maternal immune regulation. The expression of gal-1 within the placenta is considered relevant to cell-adhesion and invasion of trophoblasts, but the role of gal-1 in the immune evasion machinery exhibited by trophoblast cells remains to be elucidated. In this study, we analyzed gal-1 expression in preimplantation human embryos and first-trimester decidua-placenta specimens and serum gal-1 levels to investigate the physiological role played by this lectin during pregnancy. The effect on human leukocyte antigen G (HLAG) expression in response to stimulation or silencing of gal-1 was also determined in the human invasive, proliferative extravillous cytotrophoblast 65 (HIPEC65) cell line. Compared with normal pregnant women, circulating gal-1 levels were significantly decreased in patients who subsequently suffered a miscarriage. Human embryos undergoing preimplantation development expressed gal-1 on the trophectoderm and inner cell mass. Furthermore, our in vitro experiments showed that exogenous gal-1 positively regulated the membrane-bound HLA-G isoforms (HLA-G1 and G2) in HIPEC65 cells, whereas endogenous gal-1 also induced expression of the soluble isoforms (HLA-G5 and -G6). Our results suggest that gal-1 plays a key role in pregnancy maternal immune regulation by modulating HLA-G expression on trophoblast cells. Circulating gal-1 levels could serve as a predictive factor for pregnancy success in early human gestation.
Fil: Tirado González, Irene. Medicine University Berlin; Alemania
Fil: Freitag, Nancy. Medicine University Berlin; Alemania
Fil: Barrientos, Gabriela Laura. Medicine University Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Shaikly, Valerie. University Of Essex; Reino Unido. Assisted Reproduction and Gynaecology Centre; Reino Unido
Fil: Nagaeva, Olga. Universidad de Umea; Suecia
Fil: Strand, Magnus. Universidad de Umea; Suecia
Fil: Kjellberg, Lennart. Universidad de Umea; Suecia
Fil: Klapp, Burghard F.. Medicine University Berlin; Alemania
Fil: Mincheva Nilsson, Lucia. Universidad de Umea; Suecia
Fil: Cohen, Marie. Laboratoire d’Hormonologie; Suiza
Fil: Blois, Sandra M.. Medicine University Berlin; Alemania
description Galectin-1 (gal-1) is expressed at the feto-maternal interface and plays a role in regulating the maternal immune response against placental alloantigens, contributing to pregnancy maintenance. Both decidua and placenta contribute to gal-1 expression and may be important for the maternal immune regulation. The expression of gal-1 within the placenta is considered relevant to cell-adhesion and invasion of trophoblasts, but the role of gal-1 in the immune evasion machinery exhibited by trophoblast cells remains to be elucidated. In this study, we analyzed gal-1 expression in preimplantation human embryos and first-trimester decidua-placenta specimens and serum gal-1 levels to investigate the physiological role played by this lectin during pregnancy. The effect on human leukocyte antigen G (HLAG) expression in response to stimulation or silencing of gal-1 was also determined in the human invasive, proliferative extravillous cytotrophoblast 65 (HIPEC65) cell line. Compared with normal pregnant women, circulating gal-1 levels were significantly decreased in patients who subsequently suffered a miscarriage. Human embryos undergoing preimplantation development expressed gal-1 on the trophectoderm and inner cell mass. Furthermore, our in vitro experiments showed that exogenous gal-1 positively regulated the membrane-bound HLA-G isoforms (HLA-G1 and G2) in HIPEC65 cells, whereas endogenous gal-1 also induced expression of the soluble isoforms (HLA-G5 and -G6). Our results suggest that gal-1 plays a key role in pregnancy maternal immune regulation by modulating HLA-G expression on trophoblast cells. Circulating gal-1 levels could serve as a predictive factor for pregnancy success in early human gestation.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15939
Tirado González, Irene; Freitag, Nancy; Barrientos, Gabriela Laura; Shaikly, Valerie; Nagaeva, Olga; et al.; Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy; Oxford University Press; Molecular Human Reproduction; 19; 1; 1-2013; 43-53
1360-9947
url http://hdl.handle.net/11336/15939
identifier_str_mv Tirado González, Irene; Freitag, Nancy; Barrientos, Gabriela Laura; Shaikly, Valerie; Nagaeva, Olga; et al.; Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy; Oxford University Press; Molecular Human Reproduction; 19; 1; 1-2013; 43-53
1360-9947
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/molehr/gas043
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/molehr/article-lookup/doi/10.1093/molehr/gas043
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083093421621248
score 13.22299

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